In vitro Propagation of Purple Nutsedge (Cyperus rotundus L.) for Useful Chemical Extraction
The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
<p>[1] I. A. Ross, Medicinal plants of the world Vol. 1: Chemical constituents,
traditional and modern medicinal uses. Humana Press Inc., New Jersey.
pp. 469, 2003.
[2] K.R. Nagulendran, S. Velavan, R. Mahesh, V. Hazeena Begum, “In
vitro antioxidant activity and total polyphenolic content of Cyperus
rotundus rhizomes.” E-J. Chem. Vol. 4, pp. 440-449, 2007.
[3] R. Yazdanparast, and A. Ardestani, “In vitro antioxidant and free radical
scavenging of Cyperus rotundus. ” J. Med. Food., vol. 10, pp. 667-674,
2007.
[4] B. Lemaure, A. Touche, I. Zbinden, J. Moulin, D. Courtois, K. Mace,
and C. Darimont, “Administration of Cyperus rotundus tubers extract
prevents weight gain in obese Zucker rats. Phytotherapy Research, vol.
21, pp. 724-730, 2007.
[5] N. B. Singh, B. N. Pandey, A. Singh, “Allelopathic effects of Cyperus
rotundus extract in vitro and ex vitro on banana.” Acta Physiol. Plant.
Vol. 31, pp. 633-638, 2009.
[6] O. Kaewmun, N. WathaNaapa, W. Ngow, and T. Pewnim,
“Biochemical characteristics of Cyperus iria and Cyperus rotundus. 31st
Congress on Science and Technology of Thailand, Suranaree University
of Technology. 18-20 October, 2005.
[7] C. C. Weng, C. L. Keng, B. P. Lim, “Detection of insect juvenile
hormone III and it’s precursors from in vitro plantlets of Cyperus
rotundus.” J. Plant Biol., vol. 47, vol. 3, pp. 187-193, 2004.
[8] K. Samariya, and R. Sarin, “Isolation and identification of flavonoids
from Cyperus rotundus Linn. in vivo and in vitro.” J. Drug Deliv.
Therap., vol. 3, no. 2, pp. 109-113, 2013.
[9] J. B. Fisher, “Callus, cell suspensions, and organogenesis in tissue
cultures of purple nutsedge (Cyperus rotundus)”. Bot. Gaz., vol. 138,
no. 3, pp. 291-297, 1997
[10] Y. Mohamed-Yasseen, and W. E. Splittstoesser, “Multiplication of
Cyperus alternifolius from axillary buds in vitro: instructive laboratory
exercise.” Plant Growth Reg. Soc. Amer., vol. 20, pp. 83-89, 1992.
[11] J. F. Benazir, B. Mathithumilan, P. Ravichandran, V. Jochebed, R.
Suganthi, and V. Manimekalai, “In vitro regeneration of nut sedge
(Cyperus pangorei Rottb).” J. Plant Biochem. Biotechnol., vol. 18, pp.
209-215, 2009.
[12] K. C. Lai, S. L. Pey, L. C. Mang, and L. B. Pey, “Establishment of
Cyperus aromaticus cell suspension cultures for the production of
Juvenile hormone III.” In Vitro Cell. Dev. Biol. Plant, vol. 46, no. 1, pp.
8-12, 2010.
[13] J. Webber, M. E. Johnston, A. H. Wearing, "High irradiance increases
organogenesis in friable callus of Caustis blakei (Cyperaceae). In Vitro
Cell. Dev. Biol.-Plant., vol. 39, no. 2, 139-141, 2003.
[14] S. M. D. Rogers, “Tissue culture and wetland establishment of the
freshwater monocots Carex, Juncus, Scirpus, and Typha.” In Vitro Cell.
Dev. Biol.-Plant. vol. 39, no. 1, pp. 1-5, 2003.
[15] T. Murashige, and F. Skoog, 1962. A revised medium for rapid growth
and bio-assay with tobacco tissue culture. Physiol. Plantarum, vol. 15,
pp. 473-497, 1962.
[16] D. B. Duncan, “Multiple range and multiple F test.” Biometrics, vol. 11,
pp. 1–42, 1955.</p>
<p>[1] I. A. Ross, Medicinal plants of the world Vol. 1: Chemical constituents,
traditional and modern medicinal uses. Humana Press Inc., New Jersey.
pp. 469, 2003.
[2] K.R. Nagulendran, S. Velavan, R. Mahesh, V. Hazeena Begum, “In
vitro antioxidant activity and total polyphenolic content of Cyperus
rotundus rhizomes.” E-J. Chem. Vol. 4, pp. 440-449, 2007.
[3] R. Yazdanparast, and A. Ardestani, “In vitro antioxidant and free radical
scavenging of Cyperus rotundus. ” J. Med. Food., vol. 10, pp. 667-674,
2007.
[4] B. Lemaure, A. Touche, I. Zbinden, J. Moulin, D. Courtois, K. Mace,
and C. Darimont, “Administration of Cyperus rotundus tubers extract
prevents weight gain in obese Zucker rats. Phytotherapy Research, vol.
21, pp. 724-730, 2007.
[5] N. B. Singh, B. N. Pandey, A. Singh, “Allelopathic effects of Cyperus
rotundus extract in vitro and ex vitro on banana.” Acta Physiol. Plant.
Vol. 31, pp. 633-638, 2009.
[6] O. Kaewmun, N. WathaNaapa, W. Ngow, and T. Pewnim,
“Biochemical characteristics of Cyperus iria and Cyperus rotundus. 31st
Congress on Science and Technology of Thailand, Suranaree University
of Technology. 18-20 October, 2005.
[7] C. C. Weng, C. L. Keng, B. P. Lim, “Detection of insect juvenile
hormone III and it’s precursors from in vitro plantlets of Cyperus
rotundus.” J. Plant Biol., vol. 47, vol. 3, pp. 187-193, 2004.
[8] K. Samariya, and R. Sarin, “Isolation and identification of flavonoids
from Cyperus rotundus Linn. in vivo and in vitro.” J. Drug Deliv.
Therap., vol. 3, no. 2, pp. 109-113, 2013.
[9] J. B. Fisher, “Callus, cell suspensions, and organogenesis in tissue
cultures of purple nutsedge (Cyperus rotundus)”. Bot. Gaz., vol. 138,
no. 3, pp. 291-297, 1997
[10] Y. Mohamed-Yasseen, and W. E. Splittstoesser, “Multiplication of
Cyperus alternifolius from axillary buds in vitro: instructive laboratory
exercise.” Plant Growth Reg. Soc. Amer., vol. 20, pp. 83-89, 1992.
[11] J. F. Benazir, B. Mathithumilan, P. Ravichandran, V. Jochebed, R.
Suganthi, and V. Manimekalai, “In vitro regeneration of nut sedge
(Cyperus pangorei Rottb).” J. Plant Biochem. Biotechnol., vol. 18, pp.
209-215, 2009.
[12] K. C. Lai, S. L. Pey, L. C. Mang, and L. B. Pey, “Establishment of
Cyperus aromaticus cell suspension cultures for the production of
Juvenile hormone III.” In Vitro Cell. Dev. Biol. Plant, vol. 46, no. 1, pp.
8-12, 2010.
[13] J. Webber, M. E. Johnston, A. H. Wearing, "High irradiance increases
organogenesis in friable callus of Caustis blakei (Cyperaceae). In Vitro
Cell. Dev. Biol.-Plant., vol. 39, no. 2, 139-141, 2003.
[14] S. M. D. Rogers, “Tissue culture and wetland establishment of the
freshwater monocots Carex, Juncus, Scirpus, and Typha.” In Vitro Cell.
Dev. Biol.-Plant. vol. 39, no. 1, pp. 1-5, 2003.
[15] T. Murashige, and F. Skoog, 1962. A revised medium for rapid growth
and bio-assay with tobacco tissue culture. Physiol. Plantarum, vol. 15,
pp. 473-497, 1962.
[16] D. B. Duncan, “Multiple range and multiple F test.” Biometrics, vol. 11,
pp. 1–42, 1955.</p>
@article{"International Journal of Biological, Life and Agricultural Sciences:63831", author = "Chockpisit Thepsithar and Nongnuch Euawong and Nukul Jonghomkajorn", title = "In vitro Propagation of Purple Nutsedge (Cyperus rotundus L.) for Useful Chemical Extraction", abstract = "The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
", keywords = "Purple nutsedge, Cyperus rotundus, multiple shoot
induction, tuber formation", volume = "7", number = "8", pages = "813-5", }
