Sterilisation of in vitro Culture Medium of Chrysanthemum by Plant Essential Oils without Autoclaving
The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
<p>[1] A. D. Russell, “Similarities and differences in the responses of
microorganisms to biocides.” Journal of Antimicrobial Chemotherapy,
vol. 52, no. 5, pp. 750–763, 2003.
[2] United State Patent 5750402. “Compositions and methods to prevent
microbial contamination of plant tissue culture media.” (Online).
Available; http://www. freepatentonline.com/ 5750402.html.
[3] U. Habiba, S. Reza, M. L. Saha, M. R. Khan, and S. Hadiuzzaman,
“Endogenous bacterial contamination during in vitro culture of table
banana: Identification and prevention.” Plant Tissue Culture, vol. 12, no.
2, pp. 117–124, 2002.
[4] S. L. Teixeira, “The development of a new protocol that uses sodium
hypochlorite to replace the autoclaving procedure for establishing axenic
in vitro banan (Musa sp.) culture.” Agricell Report, vol. 47, no. 3, pp.
17–18, 2006.
[5] T. Yanagawa, M. Nagai, T. Ogino, and M. Maeguchi, “Application of
disinfection to orchid seeds, plantlet and media as a means to prevent in
vitro contamination.” Lindleyana, vol. 10, pp. 33–36, 2006.
[6] M. Chansean, and I. Syoichi, “Conservation of Wild Orchids in
Cambodia by Simple Aseptic Culture Method.” Proceedings of NIOC,
pp. 13–19, April, 2007.
[7] W. Deein, C. Thepsithar, and P. Karawak, “Effect of disinfectants on
sterile conditions of VW medium and growth of Dendrobium seeds. The
12th National Horticulture Congress, Horticulture Toward AEC Under
Climate Changes, Bangkok, Thailand, p. 77, 9-12 May 2013.
[8] C. Thepsithar, and A. Thongpukdee. “Sterilisation of Hyponex medium
by chemicals without autoclaving and growth of Phalaenopsis
protocorms.” World Academy of Science, Engineering and Technology,
vol. 78, pp. 786–789, 2013.
[9] R. A. Sawant, and P. N. Tawar, “Use of sodium hypochlorite as medium
sterilant in sugarcane micropropagation at commercial scale.” Sugar
Tech, vol. 13, no. 1, pp. 27-35, 2011.
[10] C. Thepsithar, A, Thongpukdee, S. Bunmee, and V. Chanasak, “Effects
of sterilizing agents to microbial contamination and plant growth in
vitro. Agricultural. Sci. J., vol. 40, no. 3 (suppl.), pp. 107-110, 2009.
[11] W. Deein, C. Thepsithar, and A. Thongpukdee, “In vitro culture medium
sterilization by chemicals and essential oils without autoclaving and
growth of chrysanthemum nodes.” World Academy of Science,
Engineering and Technology, vol. 78, pp. 1014–1017, 2013.
[12] M. M. Hoque, S. Rattila, M. A. Shishir, M. L. Bari, Y. Inatsu, and S.
Kawamoto, “Antibacterial activity of ethanol extract of betel leaf (Piper
betle L.) against some food borne pathogens.” Bangladesh Journal of
Microbiology, vol. 28, no. 1, 58–63, 2011.
[13] M. Seema, S. S. Sreenivas, N. D. Rekha, and N. S. Devaki, “In vitro
studies of some plant extracts against Rhizoctonia solani Kuhn infecting
FCV tobacco in Karnataka Light Soil, Karnataka, India.” Journal of
Agricultural technology, vol. 7, no. 5, pp. 1321–1329, 2011.
[14] D. Srichana, A. Phumruang, A. and Chongkid, B. “Inhibition effect of
betel leaf extract on the growth of Aspergillus flavus and Fusarium
verticillioides.” Thammasat International Journal of Science and
Technology, vol. 14, no. 3, pp. 74–77, 2009.
[15] T. S. A. T. Kamazeri, O. A. Samah, M. Taher, D. Susanti, and H.
Qaralleh, “Antimicrobial activity and essential oils of Curcuma
aerugonosa, Curcuma mangga and Zingiber cassumunar from
Malaysia.” Asian Pacific Journal of Tropical Medicine, vol. 5, no. 3,
202–209, March, 2012.
[16] B. Joshi, G. P. Sah, B. B. Basnet, M. R. Bhatt, D. Sharma, K. Subedi, J.
Pandey, and R. Malla, “Phytochemical extraction and antimicrobial
properties of different medicinal plants: Ocimum sanctum (tulsi),
Eugenia caryophyllata (clove), Achyranthes bidentata (datiwan) and
Azadirachta indica (neem).” Journal of Microbiology and
Antimicrobials, vol. 3, no. 1, pp. 1–7, January, 2011.
[17] I. N. Fit, G. Rapuntean, S. Rapuntean, F. Chirila, and G. C. Nadas,
“Antibacterial effect of essential vegetal extracts on Staphylococcus
aureus compared to antibiotics.” Notulae Botanicae Horti Agrobotanici
Cluj-Napoca, vol. 37, 117–223, 2009.
[18] L. Hui, L. He, L. Huan, L. Xiaolan, and Z. Aiguo, “Chemical
composition of lavender essential oil and its antioxidant activity and
inhibition against rhinitis related bacteria.” African Journal of
Microbiology Research, vol. 4, pp. 309–313, 2010.
[19] F. G. Kirbaslar, A. Tavman, B. Dulger, and G. Turker, “Antimicrobial
activity of Turkish Citrus peel oils.” Pakistan Journal of Botany, vol.
41, pp. 3207–3212, 2009.
[20] S. S. Allawi, J. M. Auda, H. Q. Hameed, and T. I. Ali, “The effect of
Curcuma longa (turmeric) rhizomes extracts on pathogenic bacteria in
comparison with standard antibiotics.” Journal of Biotechnology
Research Center 3, pp. 15–20, 2009.
[21] T. Murashige, and F, Skoog, “A revised medium for rapid growth and
bioassays with tobacco tissue culture.” Physiologia Plantarum, vol. 15,
no. 3, pp. 473–497, 1962.
[22] D. B. Duncan, “Multiple range and multiple F test. Biometrics, vol. 11,
pp. 1–42, 1995.
[23] S. Ranjan, N. Dasgupta, P. Saha, M. Rakshit, and C. Ramalingam,
“Comparative study of antibacterial activity of garlic and cinnamon at
different temperature and its application on preservation of fish.
Advances in Applied Science Research, vol. 3, no. 1, pp. 495-501, 2012.</p>
<p>[1] A. D. Russell, “Similarities and differences in the responses of
microorganisms to biocides.” Journal of Antimicrobial Chemotherapy,
vol. 52, no. 5, pp. 750–763, 2003.
[2] United State Patent 5750402. “Compositions and methods to prevent
microbial contamination of plant tissue culture media.” (Online).
Available; http://www. freepatentonline.com/ 5750402.html.
[3] U. Habiba, S. Reza, M. L. Saha, M. R. Khan, and S. Hadiuzzaman,
“Endogenous bacterial contamination during in vitro culture of table
banana: Identification and prevention.” Plant Tissue Culture, vol. 12, no.
2, pp. 117–124, 2002.
[4] S. L. Teixeira, “The development of a new protocol that uses sodium
hypochlorite to replace the autoclaving procedure for establishing axenic
in vitro banan (Musa sp.) culture.” Agricell Report, vol. 47, no. 3, pp.
17–18, 2006.
[5] T. Yanagawa, M. Nagai, T. Ogino, and M. Maeguchi, “Application of
disinfection to orchid seeds, plantlet and media as a means to prevent in
vitro contamination.” Lindleyana, vol. 10, pp. 33–36, 2006.
[6] M. Chansean, and I. Syoichi, “Conservation of Wild Orchids in
Cambodia by Simple Aseptic Culture Method.” Proceedings of NIOC,
pp. 13–19, April, 2007.
[7] W. Deein, C. Thepsithar, and P. Karawak, “Effect of disinfectants on
sterile conditions of VW medium and growth of Dendrobium seeds. The
12th National Horticulture Congress, Horticulture Toward AEC Under
Climate Changes, Bangkok, Thailand, p. 77, 9-12 May 2013.
[8] C. Thepsithar, and A. Thongpukdee. “Sterilisation of Hyponex medium
by chemicals without autoclaving and growth of Phalaenopsis
protocorms.” World Academy of Science, Engineering and Technology,
vol. 78, pp. 786–789, 2013.
[9] R. A. Sawant, and P. N. Tawar, “Use of sodium hypochlorite as medium
sterilant in sugarcane micropropagation at commercial scale.” Sugar
Tech, vol. 13, no. 1, pp. 27-35, 2011.
[10] C. Thepsithar, A, Thongpukdee, S. Bunmee, and V. Chanasak, “Effects
of sterilizing agents to microbial contamination and plant growth in
vitro. Agricultural. Sci. J., vol. 40, no. 3 (suppl.), pp. 107-110, 2009.
[11] W. Deein, C. Thepsithar, and A. Thongpukdee, “In vitro culture medium
sterilization by chemicals and essential oils without autoclaving and
growth of chrysanthemum nodes.” World Academy of Science,
Engineering and Technology, vol. 78, pp. 1014–1017, 2013.
[12] M. M. Hoque, S. Rattila, M. A. Shishir, M. L. Bari, Y. Inatsu, and S.
Kawamoto, “Antibacterial activity of ethanol extract of betel leaf (Piper
betle L.) against some food borne pathogens.” Bangladesh Journal of
Microbiology, vol. 28, no. 1, 58–63, 2011.
[13] M. Seema, S. S. Sreenivas, N. D. Rekha, and N. S. Devaki, “In vitro
studies of some plant extracts against Rhizoctonia solani Kuhn infecting
FCV tobacco in Karnataka Light Soil, Karnataka, India.” Journal of
Agricultural technology, vol. 7, no. 5, pp. 1321–1329, 2011.
[14] D. Srichana, A. Phumruang, A. and Chongkid, B. “Inhibition effect of
betel leaf extract on the growth of Aspergillus flavus and Fusarium
verticillioides.” Thammasat International Journal of Science and
Technology, vol. 14, no. 3, pp. 74–77, 2009.
[15] T. S. A. T. Kamazeri, O. A. Samah, M. Taher, D. Susanti, and H.
Qaralleh, “Antimicrobial activity and essential oils of Curcuma
aerugonosa, Curcuma mangga and Zingiber cassumunar from
Malaysia.” Asian Pacific Journal of Tropical Medicine, vol. 5, no. 3,
202–209, March, 2012.
[16] B. Joshi, G. P. Sah, B. B. Basnet, M. R. Bhatt, D. Sharma, K. Subedi, J.
Pandey, and R. Malla, “Phytochemical extraction and antimicrobial
properties of different medicinal plants: Ocimum sanctum (tulsi),
Eugenia caryophyllata (clove), Achyranthes bidentata (datiwan) and
Azadirachta indica (neem).” Journal of Microbiology and
Antimicrobials, vol. 3, no. 1, pp. 1–7, January, 2011.
[17] I. N. Fit, G. Rapuntean, S. Rapuntean, F. Chirila, and G. C. Nadas,
“Antibacterial effect of essential vegetal extracts on Staphylococcus
aureus compared to antibiotics.” Notulae Botanicae Horti Agrobotanici
Cluj-Napoca, vol. 37, 117–223, 2009.
[18] L. Hui, L. He, L. Huan, L. Xiaolan, and Z. Aiguo, “Chemical
composition of lavender essential oil and its antioxidant activity and
inhibition against rhinitis related bacteria.” African Journal of
Microbiology Research, vol. 4, pp. 309–313, 2010.
[19] F. G. Kirbaslar, A. Tavman, B. Dulger, and G. Turker, “Antimicrobial
activity of Turkish Citrus peel oils.” Pakistan Journal of Botany, vol.
41, pp. 3207–3212, 2009.
[20] S. S. Allawi, J. M. Auda, H. Q. Hameed, and T. I. Ali, “The effect of
Curcuma longa (turmeric) rhizomes extracts on pathogenic bacteria in
comparison with standard antibiotics.” Journal of Biotechnology
Research Center 3, pp. 15–20, 2009.
[21] T. Murashige, and F, Skoog, “A revised medium for rapid growth and
bioassays with tobacco tissue culture.” Physiologia Plantarum, vol. 15,
no. 3, pp. 473–497, 1962.
[22] D. B. Duncan, “Multiple range and multiple F test. Biometrics, vol. 11,
pp. 1–42, 1995.
[23] S. Ranjan, N. Dasgupta, P. Saha, M. Rakshit, and C. Ramalingam,
“Comparative study of antibacterial activity of garlic and cinnamon at
different temperature and its application on preservation of fish.
Advances in Applied Science Research, vol. 3, no. 1, pp. 495-501, 2012.</p>
@article{"International Journal of Biological, Life and Agricultural Sciences:63724", author = "Chockpisit Thepsithar and Aree Thongpukdee and Apichya Daorat", title = "Sterilisation of in vitro Culture Medium of Chrysanthemum by Plant Essential Oils without Autoclaving", abstract = "The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
", keywords = "Sterilizing agents, essential oils, disinfectants, MS
medium, in vitro culture, chrysanthemum, sterilization of medium
without autoclaving", volume = "7", number = "8", pages = "809-4", }
