Physiological and Biochemical Responses to Drought Stress of Chickpea Genotypes
The experimental design was 4 x 5 factorial with three
replications in fully controlled research greenhouse in Department of
Soil Sciences and Plant Nutrition, Faculty of Agriculture, University
of Selcuk in the year of 2009. Determination of tolerant chickpea
genotypes to drought was made in the research. Additionally,
sophisticated effects of drought on plant growth and development,
biochemical and physical properties or physical defense mechanisms
were presented. According to the results, the primary genotypes were
Ilgın YP (0.0063 g/gh) for leaf water capacity, 22235 70.44(%) for
relative water content, 22159 (82.47%) for real water content,
22159 (5.03 mg/l) for chlorophyll a+b, Ilgın YP (125.89 nmol
H2O2.dak-1/ mg protein-1) for peroxidase, Yunak YP (769.67
unit/ mg protein-1) for superoxide dismutase, Seydişehir YP
(16.74 μg.TA-1) for proline, Gökçe (80.01 nmol H2O2.dak-1/ mg
protein-1) for catalase. Consequently, all the genotypes
increased their enzyme activity depending on the increasing of
drought stress consider with the effects of drought stress on leaf
enzyme activity. Chickpea genotypes are increasing enzyme
activity against to drought stress.
[1] O. Önder, M., Ceyhan, E. and Kahraman, A., 2011. Effects of
Agricultural Practices on Environment. Biology, Environment and
Chemistry (ICBEC 2011), Volume 24, Page 28-32, December 28-30,
Dubai, UAE.
[2] Kahraman, A., Önder, M. and Ceyhan, E., 2011. Biodiversity and
biosecurity in Turkey. Biology, Environment and Chemistry (ICBEC
2011), Volume 24, Page 33-37, December 28-30, Dubai, UAE.
[3] Ceyhan, E., Kahraman, A. and Önder, M., 2012. The effects of
environment on plant products. International Journal of Bioscience,
Biochemistry and Bioinformatics, Volume 2, no 1, Page 48-51.
[4] Kalefetoğlu, T., 2006, Nohut (Cicer arietinum L.) çeşit ve hatlarının
kuraklık stresine karşı dayanıklılığının karakterizasyonu. Haccetepe
Universiy, Institute of Life Sciences, Master Thesis. pp: 142.
[5] Clarke, J.M. and McGaig, T.N.1982, Excised-leaf water retention
capability as an indicator of drought. J. Plant Sci., 62: 571-578.
[6] Farrant, J.M., 2000, A comparison of mechanisms of desiccation
tolerance among three angiosperm resurrection plant species. Plant Eco.,
151, 29-39.
[7] Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of
photosynthetic biomembranes. Meth Enzymol, 148: 350-382.
[8] Kumar, K.B. and P.A. Khan, 1982. Peroxidase and polyphenol oxidase
in excised ragi (Eleusine coracana cv. PR 202) leaves during
senescence. Indian J. Experimental Botany, 20: 412-416.
[9] Beauchamp, C. ve Fridovich, I., 1971, Superoxide Dismutase: Improved
Assays and Applicable to Acrylamide Gels. Analytical Biochemistry.
44: 276-287 p.
[10] Bates, L.S., Waldren, R.P., Teare, I.D., 1973, Rapid determination of
free proline for water-stress studies. Plant Soil, 39, 205- 207.
[11] Bergmeyer, H.U., 1970. Methods of Enzymatic Analysis. Verlag
Chemie, Weinheim/Bergstr, German.
[12] Düzgüneş O., Kesici T., Kavuncu, O. ve Gürbüz, F. 1987, Araştırma ve
Deneme Metodlar─▒ (─░statiksel Metodlar-II). Ankara Univ.Agricultural
Fac. Press No:1021, Class Book Serial No:295. Ankara.
[13] Fu, J. and Huang, B., 2001, Involvement of antioxidants and lipid
peroxidation in the adaptation of two cool-season grasses to localized
drought stress. Environ. Exp. Bot., 45, 105-114.
[14] Egert, M. and Tevini, M. 2002, Influence of drought on some
physiological parameters symptomatic for oxidative stress in leaves of
chives (Allium schoenoprasum). Environ. Exp. Bot., 48. 43-49.
[15] Liu, F. and St├╝tzel, H. 2002, Leaf water relations of vegetable amaranth
(Amaranthus spp.) in response to soil drying. Eur. J. Agron., 16, 137-
150.
[16] Tambussi, E. A., Casadesus, J., Munné-Bosch, S., Araus, J. L., 2002,
Photoprotection in water-stressed plants of durum wheat (Triticum
turgidum var. durum): changes in chlorophyll fluorescence spectral
signature and photosynthetic pigments. Funct. Plant Biol., 29, 35-44.
[17] Anyia, A.O. and Herzog, H., 2004. Genotypic Variability in Drought
Performance and Recovery in Cowpea under Controlled Environment. J.
Agronomy & Crop Science, 190, 151ÔÇö159.
[18] Lucero, M. E., Mueller, W., Hubstenberger, J., Phillips, G. C.,
O-Connell, M. A., 1999. Tolerance to nitrogenous explosives and
metabolism of TNT by cell suspensions of Datura innoxia. In Vitro Cell
Div. Biol. Plant, 35: 480-486.
[19] Costa França, M.G., Pham-Thi, C.A.T., Pimentel, R.O.P., Rossiello, Y.,
Fodil, Z., Laffray, D., 2000, Differences in growth and water relations
among Phaseolus vulgaris cultivars in response to induced drought
stress. Environ. Exp. Bot., 43, 227-237.
[20] Soltani, A., Khooie, F. R., Ghassemi-Golezani K. and Moghaddam, M.
2000. Thresholds for chickpea leaf expansion and transpiration response
to soil water deficit. Field Crops Res., 68 (3), 205-210.
[21] Munné-Bosch, S., Jubany-Mari, T., Alegre, L., 2001, Drought-induced
senescence is characterised by a loss of antioxidant defences in
chloroplasts. Plant. Cell Environ., 24, 1319-1327.
[22] Srivalli, B., Sharma, G. and Khanna-Chopra, R., 2003. Antioxidative
defense system in an upland rice cultivar subjected to increasing
intensity of water stress followed by recovery. Physiologia Plantarum,
119: 503-512.
[23] Jung, S., 2004, Variation in antioxidant metabolism of young and mature
leaves of Arabidopsis thaliana subjected to drought. Plant Sci., 166, 459-
466.
[24] Lazaridou, M. and Koutroubas, S.D., 2004. Drought effect on water use
efficiency of berseem clover at various growth stages . Proceeding for
the 4th International Crop Science Congress, Brisbane, Australia, 26
September-1 October 2004.
[25] Jiang, H.F. and Ren, X.P., 2004, The effect on SOD activity and protein
content in groundnut leaves by drought stress. AAS, 30, 169- 174.
[26] Asada, K. and Takahashi, M., 1987, Production and scavenging of active
oxygen radicals in photosynthesis. Photoinhibition. Kyle. D.J. (ed.).
Elsevier. pp. 227-297.
[27] Ramachandra Reddy, A., Viswanatha Chaitanya, K., Vivekanandan, M.,
2004, Drought-induced responses of photosynthesis and antioxidant
metabolism in higher plants. J. Plant Physiol.,161, 1189-1202.
[28] HongBo, S., ZongSuo, L., MingAn, S., 2005, Changes of anti-oxidative
enzymes and MDA content under soil water deficits among 10 wheat
(Triticum aestivum L.) genotypes at maturation stage. Colloids and
Surfaces B: Biointerfaces, 45, 7-13.
[29] Türkan, İ., Bor. M., Özdemir, F., Koca, H. 2005, Differential responses
of lipid peroxidation and antioxidants in the leaves of drought-tolerant P.
acutifolius Gray and drought-sensitive P. vulgaris L. subjected to
polyethylene glycol mediated water stress. Plant Sci., 168, 223-231.
[30] Ge, T., Sui, F., Bai, L., Lu, Y., Zhou, G., 2006, Effects of water stress on
the protective enzyme activities and lipid peroxidation in roots and
leaves of summer maize. ASC, 5(4), 291-298.
[31] Shao L, Young LT, Wang JF. Chronic treatment with mood stabilizers
lithium and valproate prevents excitotoxicity by inhibiting oxidative
stress in rat cerebral cortical cells. Biol Psychiatry 2005;58:879-84.
[32] Tıpırdamaz, R. ve Çakırlar. H. 1990, Buğday (Triticum aestivum L.)
bitkisinin Türkiye-de yetiştirilen iki çeşidinde tuz ve su stresinin oransal
su kapsam─▒ prolin ve betain de─ƒi┼ƒimine etkisi. DO─×A-Tr. J. of Biology.
14 ( 2), 125-148.
[33] Hsu, S.Y., Hsu, Y.T., Kao, C.H., 2003, The effect of polyethylene glycol
on proline accumulation in rice leaves. Biol. Plant., 46, 73-78.
[34] Kavi Kishore, P.B., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu,
K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu,
N., 2005, Regulation of proline biosynthesis, degradation, uptake and
transport in higher plants: its implications in plant growth and abiotic
stress tolerance. Curr. Sci., 88, 424-438.
[35] Tan, Y., Liang, Z., Shao, H., Du, F., 2006, Effect of water deficits on the
activity of anti-oxidative enzymes and osmoregulation among three
different genotypes of Radix astragali at seeding stage. Colloid. Surface.
B., 49, 59-64.
[1] O. Önder, M., Ceyhan, E. and Kahraman, A., 2011. Effects of
Agricultural Practices on Environment. Biology, Environment and
Chemistry (ICBEC 2011), Volume 24, Page 28-32, December 28-30,
Dubai, UAE.
[2] Kahraman, A., Önder, M. and Ceyhan, E., 2011. Biodiversity and
biosecurity in Turkey. Biology, Environment and Chemistry (ICBEC
2011), Volume 24, Page 33-37, December 28-30, Dubai, UAE.
[3] Ceyhan, E., Kahraman, A. and Önder, M., 2012. The effects of
environment on plant products. International Journal of Bioscience,
Biochemistry and Bioinformatics, Volume 2, no 1, Page 48-51.
[4] Kalefetoğlu, T., 2006, Nohut (Cicer arietinum L.) çeşit ve hatlarının
kuraklık stresine karşı dayanıklılığının karakterizasyonu. Haccetepe
Universiy, Institute of Life Sciences, Master Thesis. pp: 142.
[5] Clarke, J.M. and McGaig, T.N.1982, Excised-leaf water retention
capability as an indicator of drought. J. Plant Sci., 62: 571-578.
[6] Farrant, J.M., 2000, A comparison of mechanisms of desiccation
tolerance among three angiosperm resurrection plant species. Plant Eco.,
151, 29-39.
[7] Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of
photosynthetic biomembranes. Meth Enzymol, 148: 350-382.
[8] Kumar, K.B. and P.A. Khan, 1982. Peroxidase and polyphenol oxidase
in excised ragi (Eleusine coracana cv. PR 202) leaves during
senescence. Indian J. Experimental Botany, 20: 412-416.
[9] Beauchamp, C. ve Fridovich, I., 1971, Superoxide Dismutase: Improved
Assays and Applicable to Acrylamide Gels. Analytical Biochemistry.
44: 276-287 p.
[10] Bates, L.S., Waldren, R.P., Teare, I.D., 1973, Rapid determination of
free proline for water-stress studies. Plant Soil, 39, 205- 207.
[11] Bergmeyer, H.U., 1970. Methods of Enzymatic Analysis. Verlag
Chemie, Weinheim/Bergstr, German.
[12] Düzgüneş O., Kesici T., Kavuncu, O. ve Gürbüz, F. 1987, Araştırma ve
Deneme Metodlar─▒ (─░statiksel Metodlar-II). Ankara Univ.Agricultural
Fac. Press No:1021, Class Book Serial No:295. Ankara.
[13] Fu, J. and Huang, B., 2001, Involvement of antioxidants and lipid
peroxidation in the adaptation of two cool-season grasses to localized
drought stress. Environ. Exp. Bot., 45, 105-114.
[14] Egert, M. and Tevini, M. 2002, Influence of drought on some
physiological parameters symptomatic for oxidative stress in leaves of
chives (Allium schoenoprasum). Environ. Exp. Bot., 48. 43-49.
[15] Liu, F. and St├╝tzel, H. 2002, Leaf water relations of vegetable amaranth
(Amaranthus spp.) in response to soil drying. Eur. J. Agron., 16, 137-
150.
[16] Tambussi, E. A., Casadesus, J., Munné-Bosch, S., Araus, J. L., 2002,
Photoprotection in water-stressed plants of durum wheat (Triticum
turgidum var. durum): changes in chlorophyll fluorescence spectral
signature and photosynthetic pigments. Funct. Plant Biol., 29, 35-44.
[17] Anyia, A.O. and Herzog, H., 2004. Genotypic Variability in Drought
Performance and Recovery in Cowpea under Controlled Environment. J.
Agronomy & Crop Science, 190, 151ÔÇö159.
[18] Lucero, M. E., Mueller, W., Hubstenberger, J., Phillips, G. C.,
O-Connell, M. A., 1999. Tolerance to nitrogenous explosives and
metabolism of TNT by cell suspensions of Datura innoxia. In Vitro Cell
Div. Biol. Plant, 35: 480-486.
[19] Costa França, M.G., Pham-Thi, C.A.T., Pimentel, R.O.P., Rossiello, Y.,
Fodil, Z., Laffray, D., 2000, Differences in growth and water relations
among Phaseolus vulgaris cultivars in response to induced drought
stress. Environ. Exp. Bot., 43, 227-237.
[20] Soltani, A., Khooie, F. R., Ghassemi-Golezani K. and Moghaddam, M.
2000. Thresholds for chickpea leaf expansion and transpiration response
to soil water deficit. Field Crops Res., 68 (3), 205-210.
[21] Munné-Bosch, S., Jubany-Mari, T., Alegre, L., 2001, Drought-induced
senescence is characterised by a loss of antioxidant defences in
chloroplasts. Plant. Cell Environ., 24, 1319-1327.
[22] Srivalli, B., Sharma, G. and Khanna-Chopra, R., 2003. Antioxidative
defense system in an upland rice cultivar subjected to increasing
intensity of water stress followed by recovery. Physiologia Plantarum,
119: 503-512.
[23] Jung, S., 2004, Variation in antioxidant metabolism of young and mature
leaves of Arabidopsis thaliana subjected to drought. Plant Sci., 166, 459-
466.
[24] Lazaridou, M. and Koutroubas, S.D., 2004. Drought effect on water use
efficiency of berseem clover at various growth stages . Proceeding for
the 4th International Crop Science Congress, Brisbane, Australia, 26
September-1 October 2004.
[25] Jiang, H.F. and Ren, X.P., 2004, The effect on SOD activity and protein
content in groundnut leaves by drought stress. AAS, 30, 169- 174.
[26] Asada, K. and Takahashi, M., 1987, Production and scavenging of active
oxygen radicals in photosynthesis. Photoinhibition. Kyle. D.J. (ed.).
Elsevier. pp. 227-297.
[27] Ramachandra Reddy, A., Viswanatha Chaitanya, K., Vivekanandan, M.,
2004, Drought-induced responses of photosynthesis and antioxidant
metabolism in higher plants. J. Plant Physiol.,161, 1189-1202.
[28] HongBo, S., ZongSuo, L., MingAn, S., 2005, Changes of anti-oxidative
enzymes and MDA content under soil water deficits among 10 wheat
(Triticum aestivum L.) genotypes at maturation stage. Colloids and
Surfaces B: Biointerfaces, 45, 7-13.
[29] Türkan, İ., Bor. M., Özdemir, F., Koca, H. 2005, Differential responses
of lipid peroxidation and antioxidants in the leaves of drought-tolerant P.
acutifolius Gray and drought-sensitive P. vulgaris L. subjected to
polyethylene glycol mediated water stress. Plant Sci., 168, 223-231.
[30] Ge, T., Sui, F., Bai, L., Lu, Y., Zhou, G., 2006, Effects of water stress on
the protective enzyme activities and lipid peroxidation in roots and
leaves of summer maize. ASC, 5(4), 291-298.
[31] Shao L, Young LT, Wang JF. Chronic treatment with mood stabilizers
lithium and valproate prevents excitotoxicity by inhibiting oxidative
stress in rat cerebral cortical cells. Biol Psychiatry 2005;58:879-84.
[32] Tıpırdamaz, R. ve Çakırlar. H. 1990, Buğday (Triticum aestivum L.)
bitkisinin Türkiye-de yetiştirilen iki çeşidinde tuz ve su stresinin oransal
su kapsam─▒ prolin ve betain de─ƒi┼ƒimine etkisi. DO─×A-Tr. J. of Biology.
14 ( 2), 125-148.
[33] Hsu, S.Y., Hsu, Y.T., Kao, C.H., 2003, The effect of polyethylene glycol
on proline accumulation in rice leaves. Biol. Plant., 46, 73-78.
[34] Kavi Kishore, P.B., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu,
K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu,
N., 2005, Regulation of proline biosynthesis, degradation, uptake and
transport in higher plants: its implications in plant growth and abiotic
stress tolerance. Curr. Sci., 88, 424-438.
[35] Tan, Y., Liang, Z., Shao, H., Du, F., 2006, Effect of water deficits on the
activity of anti-oxidative enzymes and osmoregulation among three
different genotypes of Radix astragali at seeding stage. Colloid. Surface.
B., 49, 59-64.
@article{"International Journal of Biological, Life and Agricultural Sciences:63751", author = "E. Ceyhan and A. Kahraman and M. Önder and M.K. Ateş and S. Karadaş and R. Topak and M.A. Avcı", title = "Physiological and Biochemical Responses to Drought Stress of Chickpea Genotypes", abstract = "The experimental design was 4 x 5 factorial with three
replications in fully controlled research greenhouse in Department of
Soil Sciences and Plant Nutrition, Faculty of Agriculture, University
of Selcuk in the year of 2009. Determination of tolerant chickpea
genotypes to drought was made in the research. Additionally,
sophisticated effects of drought on plant growth and development,
biochemical and physical properties or physical defense mechanisms
were presented. According to the results, the primary genotypes were
Ilgın YP (0.0063 g/gh) for leaf water capacity, 22235 70.44(%) for
relative water content, 22159 (82.47%) for real water content,
22159 (5.03 mg/l) for chlorophyll a+b, Ilgın YP (125.89 nmol
H2O2.dak-1/ mg protein-1) for peroxidase, Yunak YP (769.67
unit/ mg protein-1) for superoxide dismutase, Seydişehir YP
(16.74 μg.TA-1) for proline, Gökçe (80.01 nmol H2O2.dak-1/ mg
protein-1) for catalase. Consequently, all the genotypes
increased their enzyme activity depending on the increasing of
drought stress consider with the effects of drought stress on leaf
enzyme activity. Chickpea genotypes are increasing enzyme
activity against to drought stress.", keywords = "Chickpea, drought, enzyme, tolerance to drought", volume = "6", number = "6", pages = "389-6", }
