Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: Fungal mutant strains have produced cellulase and
xylanase enzymes, and have induced high hydrolysis with enhanced
of rice straw. The mutants were obtained by exposing Penicillium
strain to UV-light treatments. Screening and selection after treatment
with UV-light were carried out using cellulolytic and xylanolytic
clear zones method to select the hypercellulolytic and
hyperxylanolytic mutants. These mutants were evaluated for their
cellulase and xylanase enzyme production as well as their abilities for
biodegradation of rice straw. The mutant 12 UV/1 produced 306.21%
and 209.91% cellulase and xylanase, respectively, as compared with
the original wild type strain. This mutant showed high capacity of
rice straw degradation. The effectiveness of tested mutant strain and
that of wild strain was compared in relation to enhancing the
composting process of rice straw and animal manures mixture. The
results obtained showed that the compost product of inoculated
mixture with mutant strain (12 UV/1) was the best compared to the
wild strain and un-inoculated mixture. Analysis of the composted
materials showed that the characteristics of the produced compost
were close to those of the high quality standard compost. The results
obtained in the present work suggest that the combination between
rice straw and animal manure could be used for enhancing the
composting process of rice straw and particularly when applied with
fungal decomposer accelerating the composting process.
Abstract: Total hip replacement had been one of the most
successful operations in hip arthritis surgery. The purpose of this
research had been to develop a dynamic hip contact of Thai femoral
bone to analyze the stress distribution on the implant and the strain
distribution on the bone model under daily activities and compared
with the static load simulation. The results showed the different of
maximum von Mises stress 0.14 percent under walking and 0.03
percent under climbing stair condition and the different of equivalent
total strain 0.52 percent under walking and 0.05 percent under
climbing stair condition. The muscular forces should be evaluated
with dynamic condition to reduce the maximum von Mises stress and
equivalent total strain.
Abstract: Fiber Bragg optic sensor is embedded in composite
material to detect and monitor the damage that occurs in composite
structures. In this paper, we deal with the mode-Ι delamination to
determine the material strength to crack propagation, using the
coupling mode theory and T-matrix method to simulate the FBGs
spectrum for both uniform and non-uniform strain distribution. The
double cantilever beam test is modeled in FEM to determine the
longitudinal strain. Two models are implemented, the first is the
global half model, and the second is the sub-model to represent the
FBGs with higher refined mesh. This method can simulate damage in
composite structures and converting strain to a wavelength shifting in
the FBG spectrum.
Abstract: In this paper, a nonlinear Finite Element Analysis
(FEA) was carried out using ANSYS software to build a model able
of predicting the behavior of Reinforced Concrete (RC) beams with
unbonded reinforcement. The FEA model was compared to existing
experimental data by other researchers. The existing experimental
data consisted of 16 beams that varied from structurally sound beams
to beams with unbonded reinforcement with different unbonded
lengths and reinforcement ratios. The model was able to predict the
ultimate flexural strength, load-deflection curve, and crack pattern of
concrete beams with unbonded reinforcement. It was concluded that
when the when the unbonded length is less than 45% of the span,
there will be no decrease in the ultimate flexural strength due to the
loss of bond between the steel reinforcement and the surrounding
concrete regardless of the reinforcement ratio. Moreover, when the
reinforcement ratio is relatively low, there will be no decrease in
ultimate flexural strength regardless of the length of unbond.
Abstract: The lactic acid bacteria (LAB) were isolated from
10 samples of fermented foods (Sa-tor-dong and Bodo) in South
locality of Thailand. The 23 isolates of lactic acid bacteria were
selected, which were exhibited a clear zone and growth on MRS
agar supplemented with CaCO3. All of lactic acid bacteria were
tested on morphological and biochemical. The result showed that
all isolates were Gram’s positive, non-spore forming but only
10 isolates displayed catalase negative. The 10 isolates including
BD1 .1, BD 1.2, BD 2.1, BD2.2, BD 2.3, BD 3.1, BD 4.1, BD 5.2,
ST 4.1 and ST 5.2 were selected for inhibition activity
determination. Only 2 strains (ST 4.1 and BD 2.3) showed
inhibition zone on agar, when using Escherichia coli sp. as target
strain. The ST 4.1 showed highest inhibition zone on agar, which
was selected for probiotic property testing. The ST4.1 isolate
could grow in MRS broth containing a high concentration of
sodium chloride 6%, bile salts 7%, pH 4-10 and vary temperature
at 15-45°C.
Abstract: Each year nearly nine hundred persons die in head injuries and over fifty thousand persons are severely injured due to non wearing of helmets. In motor cycle accidents, the human head is exposed to heavy impact loading against natural protection. In this work, an attempt has been made for analyzing the helmet with all the standard data. The simulation software ‘ANSYS’ is used to analyze the helmet with different conditions such as bottom fixed-load on top surface, bottom fixed -load on top line, side fixed –load on opposite surface, side fixed-load on opposite line and dynamic analysis. The maximum force of 19.5 kN is applied on the helmet to study the model in static and dynamic conditions. The simulation has been carried out for the static condition for the parameters like total deformation, strain energy, von-Mises stress for different cases. The dynamic analysis has been performed for the parameter like total deformation and equivalent elastic strain. The result shows that these values are concentrated in the retention portion of the helmet. These results have been compared with the standard experimental data proposed by the BIS and well within the acceptable limit.
Abstract: The waste agriculture materials cause environment pollution, recycle of these materials help sustainable development. This study focused on the impact of used oil palm shell ash on the compressive and flexural strengths of cement mortar. Two different cement mortar mixes have been designed to investigate the impact of oil palm shell ash on strengths of cement mortar. Quantity of 4% oil palm shell ash has been replaced in cement mortar. The main objective of this paper is, to modify mechanical properties of cement mortar by replacement of oil palm ash in it at early age of 7 days. The results have been revealed optimum quantity of oil palm ash for replacement in cement mortar. The deflection, load to failure, time to failure of compressive strength and flexural strength of all specimens have significantly been improved. The stress-strain behavior has been indicated ability of modified cement mortar in control stress path and strain. The micro property of cement paste has not been investigated.
Abstract: Studies were carried out on the comparative study of the production of Avicelase enzyme using sugarcane bagasse-SCB in two different statuses (i.e. treated and untreated SCB) by thermophilic Geobacillus stearothermophilus at 50ºC. Only four thermophilic bacterial isolates were isolated and assayed for Avicelase production using UntSCB and TSCB. Only one isolate selected as most potent and identified as G. stearothermophilus used in this study. A specific endo-β-1,4-D-glucanase (Avicelase EC 3.2.1.91) was partially purified from a thermophilic bacterial strain was isolated from different soil samples when grown on cellulose enrichment SCB substrate as the sole carbon source. Results shown that G. stearothermophilus was the better Avicelase producer strain. Avicelase had an optimum pH and temperature 7.0 and 50ºC for both UntSCB and TSCB and exhibited good pH stability between "5-8" and "4-9", however, good temperature stability between (30-80ºC) for UntSCB and TSCB, respectively. Other factors affecting the production of Avicelase were compared (i.e. SCB concentration, inoculum size and different incubation periods), all results observed and obtained were revealed that the TSCB was exhibited maximal enzyme activity in comparison with the results obtained from UntSCB, so, the TSCB was enhancing the Avicelase production.
Abstract: The objective of this research was to investigate biodegradation of water hyacinth (Eichhornia crassipes) to produce bioethanol using dilute-acid pretreatment (1% sulfuric acid) results in high hemicellulose decomposition and using yeast (Pachysolen tannophilus) as bioethanol producing strain. A maximum ethanol yield of 1.14g/L with coefficient, 0.24g g-1; productivity, 0.015g l-1h-1 was comparable to predicted value 32.05g/L obtained by Central Composite Design (CCD). Maximum ethanol yield coefficient was comparable to those obtained through enzymatic saccharification and fermentation of acid hydrolysate using fully equipped fermentor. Although maximum ethanol concentration was low in lab scale, the improvement of lignocellulosic ethanol yield is necessary for large scale production.
Abstract: The mechanical deformation and the electrical conductivity of lanthanum strontium cobalt ferrite oxide under uniaxial compression were investigated at various temperatures up to 1073 K. The material reveals a rather complex mechanical behaviour related to its ferroelasticity and completely different stress-strain curves are obtained during the 1st and 2nd loading cycles. A distinctive ferroelastic creep was observed at 293 K whilst typical ferroelastic stress-strain curve were obtained in the temperature range from 473 K to 873 K. At 1073 K, on the other hand, high-temperature creep deformation was observed instead of ferroelastic deformation. The conductivity increases with increasing compressive stress at all the temperatures. The increase in conductivity is related to both geometrical and piezoelectric effects. From 293 K to 873 K, where the material exhibits ferroelastic behaviour, the variation in the total conductivity decreases with increasing temperature. The contribution of the piezoelectric effect to the total conductivity variation also decreases with increasing temperature and the maximum in piezoconductivity has a value of about 0.75 % at 293 K for a compressive stress of 100 MPa. There is no effect of domain switching on conductivity except for the geometric effect. At 1073 K, the conductivity is simply proportional to the compressive strain.
Abstract: Metal cutting is a severe plastic deformation process
involving large strains, high strain rates, and high temperatures.
Conventional analysis of the chip formation process is based on bulk
material deformation disregarding the inhomogeneous nature of the
material microstructure. A series of orthogonal cutting tests of AISI
1045 and 1144 steel were conducted which yielded similar process
characteristics and chip formations. With similar shear angles and cut
chip thicknesses, shear strains for both chips were found to range
from 2.0 up to 2.8. The manganese-sulfide (MnS) precipitate in the
1144 steel has a very distinct and uniform shape which allows for
comparison before and after chip formation. From close observations
of MnS precipitates in the cut chips it is shown that the conventional
approach underestimates plastic strains in metal cutting.
Experimental findings revealed local shear strains around a value of
6. These findings and their implications are presented and discussed.
Abstract: This study include the effect of strain and storage
period and their interaction on some quantitative and qualitative traits
and percentages of the egg components in the eggs collected at the
start of production (at age 24 weeks). Eggs were divided into three
storage periods (1, 7 and 14) days under refrigerator temperature (5-
7)0C. Fifty seven eggs obtained randomly from each strain including
Isa Brown and Lohman White. General Linear Model within
SAS programme was used to analyze the collected data
and correlations between the studied traits were calculated for each
strain.Average egg weight (EW), Haugh Unit (HU), yolk index (YI),
yolk % (HP), albumin % (AP) and yolk to albumin ratio (YAR) was
56.629 gm, 87.968 %, 0.493, 22.13%, 67.74% and 32.76
respectively. Egg produced from ISA Brown surpassed those
produced by Lohman White significantly (P
Abstract: This is the first report from India on a beverage resulting from alcoholic fermentation of the juice of sea buckthorn (Hippophae rhamnoides L) using lab isolated yeast strain. The health promoting potential of the product was evaluated based on its total phenolic content. The most important finding was that under the present fermentation condition, the total phenolic content of the wine product was 689 mg GAE/L. Investigation of influence of bottle ageing on the sea buckthorn wine showed a slight decrease in the phenolic content (534 m mg GAE/L). This study also includes the comparative analysis of the phenolic content of wines from other selected fruit juices like grape, apple and black currant. KeywordsAlcoholic fermentation, Hippophae, Total phenolic content, Wine
Abstract: Although considerable amount of research has attested to the link between work-to-family conflict (WFC) and family-to-work conflict (FWC) and psychological strain and wellbeing, there is a paucity of research investigating the phenomenon in the context of social workers. Moreover, very little is known about the impact of WFC and FWC in developing countries. The present study investigated the mediating effect of psychological strain on the relationship between WFC and FWC with wellbeing of social workers in India. Our findings show that WFC and FWC are influential antecedents of wellbeing; their influence is both direct on psychological strain, and indirect on wellbeing transmitted through psychological strain. Implications of the findings are discussed.
Abstract: The present paper concerns with the influence of fiber
packing on the transverse plastic properties of metal matrix
composites. A micromechanical modeling procedure is used to
predict the effective mechanical properties of composite materials at
large tensile and compressive deformations. Microstructure is
represented by a repeating unit cell (RUC). Two fiber arrays are
considered including ideal square fiber packing and random fiber
packing defined by random sequential algorithm. The
micromechanical modeling procedure is implemented for
graphite/aluminum metal matrix composite in which the
reinforcement behaves as elastic, isotropic solids and the matrix is
modeled as an isotropic elastic-plastic solid following the von Mises
criterion with isotropic hardening and the Ramberg-Osgood
relationship between equivalent true stress and logarithmic strain.
The deformation is increased to a considerable value to evaluate both
elastic and plastic behaviors of metal matrix composites. The yields
strength and true elastic-plastic stress are determined for
graphite/aluminum composites.
Abstract: To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.
Abstract: Silver nanoparticles were prepared by chemical reduction method. Silver nitrate was taken as the metal precursor and hydrazine hydrate as a reducing agent. The formation of the silver nanoparticles was monitored using UV-Vis absorption spectroscopy. The UV-Vis spectroscopy revealed the formation of silver nanopart├¡cles by exhibing the typical surface plasmon absorption maxima at 418-420 nm from the UV–Vis spectrum. Comparison of theoretical (Mie light scattering theory) and experimental results showed that diameter of silver nanoparticles in colloidal solution is about 60 nm. We have used energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD), transmission electron microscopy (TEM) and, UV–Vis spectroscopy to characterize the nanoparticles obtained. The energy-dispersive spectroscopy (EDX) of the nanoparticles dispersion confirmed the presence of elemental silver signal no peaks of other impurity were detected. The average size and morphology of silver nanoparticles were determined by transmission electron microscopy (TEM). TEM photographs indicate that the nanopowders consist of well dispersed agglomerates of grains with a narrow size distribution (40 and 60 nm), whereas the radius of the individual particles are between 10 and 20 nm. The synthesized nanoparticles have been structurally characterized by X-ray diffraction and transmission high-energy electron diffraction (HEED). The peaks in the XRD pattern are in good agreement with the standard values of the face-centered-cubic form of metallic silver (ICCD-JCPDS card no. 4-0787) and no peaks of other impurity crystalline phases were detected. Additionally, the antibacterial activity of the nanopart├¡culas dispersion was measured by Kirby-Bauer method. The nanoparticles of silver showed high antimicrobial and bactericidal activity against gram positive bacteria such as Escherichia Coli, Pseudimonas aureginosa and staphylococcus aureus which is a highly methicillin resistant strain.
Abstract: The main objectives of this study are to inspect and
identify any damage of jaimusi highway prestressed concrete bridge
after repair and strengthening of damaged structural members and to
evaluate the performance of the bridge structural members by
adopting static load test. Inspection program after repair and
strengthening includes identifying and evaluating the structural
members of bridge such as T-shape cantilever structure, hanging
beams, corbels, external tendons, anchor beams, sticking steel plate,
and piers. The results of inspection show that the overall state of the
bridge structural member after repair and strengthening is good. The
results of rebound test of concrete strength show that the average
strength of concrete is 46.31Mpa. Whereas, the average value of
concrete strength of anchor beam is 49.82Mpa. According to the
results of static load test, the experimental values are less than
theoretical values of internal forces, deflection, and strain, indicating
that the stiffness of the experimental structure, overall deformation
and integrity satisfy the designed standard and the working
performance is good, and the undertaking capacity has a certain
surplus. There is not visible change in the length and width of cracks
and there are not new cracks under experimental load.
Abstract: Aluminum alloy sheets have several advantages such
as the lightweight, high-specific strength and recycling efficiency.
Therefore, aluminum alloy sheets in sheet forming have been used in various areas as automotive components and so forth. During the
process of sheet forming, wrinkling which is caused by compression stress might occur and the formability of sheets was affected by
occurrence of wrinkling. A few studies of uniaxial compressive test by
using square tubes, pipes and sheets were carried out to clarify the each wrinkling behavior. However, on uniaxial compressive test,
deformation behavior of the sheets hasn-t be cleared. Then, it is necessary to clarify the relationship between the buckling behavior
and the forming conditions. In this study, the effect of dimension of the sheet in the buckling behavior on compression test of aluminum alloy sheet was cleared by experiment and FEA. As the results, the buckling
deformation was classified by three modes in terms of the distribution of equivalent plastic strain.