Abstract: This study presents an attempt to evaluate the
antioxidant potential and antimicrobial activity of methanolic extract,
and essential oils prepared from the leaves of sage (Salvia officinalis
L.). The content of polyphenol in the methanolic extracts from the
leaves of Salvia officinalis was determined spectrophotometrically,
calculated as gallic acid and catechin equivalent. The essential oils
and methanol extract were also subjected to screenings for the
evaluation of their antioxidant activities using 2, 2-diphenyl-1-
picrylhydrazyl (DPPH) test. While the plant essential oils showed
only weak antioxidant activities, its methanol extract was
considerably active in DPPH (IC50 = 37.29 μg/ml) test. Appreciable
total polyphenol content (31.25 mg/g) was also detected for the plant
methanol extract as gallic acid equivalent in the Folin–Ciocalteu test.
The plant was also screened for its antimicrobial activity and good to
moderate inhibitions were recorded for its essential oils, and
methanol extracts against most of the tested microorganisms.
The present investigation revealed that this plant had rich source
of antioxidant properties. It is for this reason that sage has found
increasing application in food formulations.
Abstract: Essential oils are expensive phytochemicals produced
and extracted from specific species belonging to particular families in
the plant kingdom. In the United Arab Emirates country (UAE), is
located in the arid region of the world, nine species, from the
Lamiaceae family, having the capability to produce therapeutic grade
essential oils. These species include; Mentha spicata, Ocimum
forskolei, Salvia macrosiphon, Salvia aegyptiaca, Salvia macilenta,
Salvia spinosa, Teucrium polium, Teucrium stocksianum and Zataria
multiflora. Although, such potential species are indigenous to the
UAE, however, there are almost no studies available to investigate
the chemical composition and the quality of the extracted essential
oils under the UAE climatological conditions. Therefore, great
attention has to be given to such valuable natural resources, through
conducting highly supported research projects, tailored to the UAE
conditions, and investigating different extraction techniques,
including the application of the latest available technologies, such as
superficial fluid CO2. This is crucially needed; in order to accomplish
the greatest possibilities in the medicinal field, specifically in the
discovery of new therapeutic chemotypes, as well as, to achieve the
sustainability of this natural resource in the country.
Abstract: Humans use plants for thousands of years to treat various ailments, in many developing countries; much of the population relies on traditional doctors and their collections of medicinal plants to cure them.
Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin.
The aim of our study is to determine the antimicrobial effect of essential oils of the plant Schinus molle on some pathogenic bacteria. It is a medicinal plant used in traditional therapy. Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin.
The test adopted, is based on the diffusion method on solid medium (Antibiogram), this method allows to determine the susceptibility or resistance of an organism according to the sample studied.
Our study reveals that the essential oil of the plant Schinus molle has a different effect on the resistance of germs: for Pseudomonas aeruginosa strain is a moderately sensitive with an inhibition zone of 10mm, further Enterobacter, Escherichia coli and Proteus are strains that represent a high sensitivity, a zone of inhibition equal to 14.66 mm.
Abstract: The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
Abstract: Antifungal activities of ether and methanolic extracts of volatiles oils of Nigella Sativa seeds were tested against pathogenic bacterias and fungies strains.The volatile oil were found to have significant antifungal and antibacterial activities compare to tetracycline, cefuroxime and ciprofloxacin positive controls.The ether and methanolic esxtracts were compared to each other for antifungal and antibacterial activities and ether extracts showed stonger activity than methanolic one.
Abstract: Plant tissue culture is an important in vitro technology applied for agricultural and industrial production. A sterile condition of culture medium is one of the main aspects. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of plant tissue culture medium without autoclaving, ten commercial pure essential oils and 5 disinfectants were tested. Each essential oil or disinfectant was added to a 20-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils or disinfectants, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from betel oil or clove oil (18 mL/20 mL medium), cinnamon oil (36 mL/20 mL medium), lavender oil or holy basil oil (108 mL/20 mL medium), and lemon oil or tea tree oil or turmeric oil (252 mL/20 mL medium), compared to 100% sterile condition from autoclaved medium. For disinfectants, 2% iodine + 2.4% potassium iodide, 2% merbromine solution, 10% povidone-iodine, 6% sodium hypochlorite or 0.1% thimerosal at 36 mL/20 mL medium provided 100% sterile conditions. Furthermore, growth of new shoots from chrysanthemum node explants on treated media (fresh weight, shoot length, root length and number of node) were also reported and discussed in the comparison of those on autoclaved medium.
Abstract: The plant world is the source of many medicines.
Recently, researchers have estimated that there are approximately
400,000 plant species worldwide, of which about a quarter or a third
have been used by societies for medicinal purposes. The human uses
of plants for thousands of years to treat various ailments, in many
developing countries, much of the population trust in traditional
doctors and their collections of medicinal plants to treat them.
Essential oils have many therapeutic properties. In herbal medicine,
they are used for their antiseptic properties against infectious
diseases of fungal origin, against dermatophytes, those of bacterial
origin. The aim of our study is to determine the antimicrobial effect
of essential oils of the plant Trigonella focnum greacum on some
pathogenic bacteria, it is a medicinal plant used in traditional
therapy. The test adopted is based on the diffusion method on solid
medium (Antibiogram), this method determines the sensitivity or
resistance of a microorganism vis-à-vis the extract studied. Our study
reveals that the essential oil of the plant Trigonella focnum greacum
has a different effect on the resistance of germs. For staphiloccocus
Pseudomonnas aeroginosa and Krebsilla, are moderately sensitive
strains, also Escherichia coli and Candida albicans represents a high
sensitivity. By against Proteus is a strain that represents a weak
sensitivity.
Abstract: The purpose of this study was to elucidate the factors affecting antimicrobial effectiveness of essential oils against food spoilage and pathogenic bacteria. The minimum inhibition concentrations (MIC) of the essential oils, were determined by turbidimetric technique using Biocreen C, analyzer. The effects of pH ranging from 7.3 to 5.5 in absence and presence of essential oils and/or NaCl on the lag time and mean generation time of the bacteria at 370C, were carried out and results were determined showed that, combination of low pH and essential oil at 370C had additive effects against the test micro-organisms. The combination of 1.2 % (w/v) of NaCl and clove essential oil at 0.0325% (v/v) was effective against E. coli. The use of concentrations less than MIC in combination with low pH and or NaCl has the potential of being used as an alternative to “traditional food preservatives".
Abstract: This study was conducted to evaluate the antifungal
activities of Cinnamomum zeylanicum and Origanum vulgare L.
essential oil against Aspergillus flavus in culture media and tomato
paste. 200 ppm of cinnamon and 500 ppm of oregano completely
inhibited A. flavus growth in culture media, while in tomato paste 300
ppm of cinnamon and 200 ppm of oregano had the same effect. Test
panel evaluations revealed that samples with 100 and 200 ppm
cinnamon were acceptable. The results may suggest the potential use
of Cinnamomum zeylanicum essential oil as natural preservative in
tomato paste.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.
Abstract: Team distillation assisted by microwave extraction
(SDAM) considered as accelerated technique extraction is a
combination of microwave heating and steam distillation, performed
at atmospheric pressure. SDAM has been compared with the same
technique coupled with the cryogrinding of seeds (SDAM -CG).
Isolation and concentration of volatile compounds are performed by a
single stage for the extraction of essential oil from Cuminum
cyminum seeds. The essential oils extracted by these two methods for
5 min were quantitatively (yield) and qualitatively (aromatic profile)
no similar. These methods yield an essential oil with higher amounts
of more valuable oxygenated compounds, and allow substantial
savings of costs, in terms of time, energy and plant material. SDAM
and SDAM-CG is a green technology and appears as a good
alternative for the extraction of essential oils from aromatic plants.