Abstract: Non-viral gene carriers composed of biodegradable
polymers or lipids have been considered as a safer alternative for gene
carriers over viral vectors. We have developed multi-functional
nano-micelles for both drug and gene delivery application.
Polyethyleneimine (PEI) was modified by grafting stearic acid (SA)
and formulated to polymeric micelles (PEI-SA) with positive surface
charge for gene and drug delivery. Our results showed that PEI-SA
micelles provided high siRNA binding efficiency. In addition, siRNA
delivered by PEI-SA carriers also demonstrated significantly high
cellular uptake even in the presence of serum proteins. The
post-transcriptional gene silencing efficiency was greatly improved by
the polyplex formulated by 10k PEI-SA/siRNA. The amphiphilic
structure of PEI-SA micelles provided advantages for multifunctional
tasks; where the hydrophilic shell modified with cationic charges can
electrostatically interact with DNA or siRNA, and the hydrophobic
core can serve as payloads for hydrophobic drugs, making it a
promising multifunctional vehicle for both genetic and chemotherapy
application.
Abstract: We aimed to investigate how can target and optimize
pulmonary delivery distribution by changing physicochemical
characteristics of instilled liquid.Therefore, we created a new liquids
group:
a. eligible for desired distribution within lung because of
assorted physicochemical characteristics
b. capable of being augmented with a broad range of
chemicals inertly
c. no interference on respiratory function
d. compatible with airway surface liquid
We developed forty types of new liquid,were composed of
Carboxymethylcellulose sodium,Glycerin and different types of
Polysorbates.Viscosity was measured using a Programmable
Rheometer and surface tension by KRUSS Tensiometer.We
subsequently examined the liquids and delivery protocols by simple
and branched glass capillary tube models of airways.Eventually,we
explored pulmonary distribution of liquids being augmented with
technetium-99m in mechanically ventilated rabbits.We used a single
head large field of view gamma camera.Kinematic viscosity between
0.265Stokes and 0.289Stokes,density between 1g/cm3 and 1.5g/cm3
and surface tension between 25dyn/cm and 35dyn/cm were the most
acceptable.
Abstract: There are several approaches for handling multiclass classification. Aside from one-against-one (OAO) and one-against-all (OAA), hierarchical classification technique is also commonly used. A binary classification tree is a hierarchical classification structure that breaks down a k-class problem into binary sub-problems, each solved by a binary classifier. In each node, a set of classes is divided into two subsets. A good class partition should be able to group similar classes together. Many algorithms measure similarity in term of distance between class centroids. Classes are grouped together by a clustering algorithm when distances between their centroids are small. In this paper, we present a binary classification tree with tuned observation-based clustering (BCT-TOB) that finds a class partition by performing clustering on observations instead of class centroids. A merging step is introduced to merge any insignificant class split. The experiment shows that performance of BCT-TOB is comparable to other algorithms.
Abstract: In this study, the effect of nanofluids on the pool film
boiling was experimentally investigated at saturated condition under
atmospheric pressure. For this purpose, four different water-based
nanofluids (Al2O3, SiO2, TiO2 and CuO) with 0.1% particle volume
fraction were prepared. To investigate the boiling heat transfer, a
cylindrical rod with high temperature was used. The rod heated up to
high temperatures was immersed into nanofluids. The center
temperature of rod during the cooling process was recorded by using
a K-type thermocouple. The quenching curves showed that the pool
boiling heat transfer was strongly dependent on the nanoparticle
materials. During the repetitive quenching tests, the cooling time
decreased and thus, the film boiling vanished. Consequently, the
primary reason of this was the change of the surface characteristics
due to the nanoparticles deposition on the rod-s surface.
Abstract: It is important problems to increase the detection rates
and reduce false positive rates in Intrusion Detection System (IDS).
Although preventative techniques such as access control and
authentication attempt to prevent intruders, these can fail, and as a
second line of defence, intrusion detection has been introduced. Rare
events are events that occur very infrequently, detection of rare
events is a common problem in many domains. In this paper we
propose an intrusion detection method that combines Rough set and
Fuzzy Clustering. Rough set has to decrease the amount of data and
get rid of redundancy. Fuzzy c-means clustering allow objects to
belong to several clusters simultaneously, with different degrees of
membership. Our approach allows us to recognize not only known
attacks but also to detect suspicious activity that may be the result of
a new, unknown attack. The experimental results on Knowledge
Discovery and Data Mining-(KDDCup 1999) Dataset show that the
method is efficient and practical for intrusion detection systems.
Abstract: For stricter drinking water regulations in the future, reducing the humic acid and disinfection byproducts in raw water, namely, trihalomethanes (THMs) and haloacetic acids (HAAs) is worthy for research. To investigate the removal of waterborne organic material using a lab-scale of bio-activated carbon filter under different EBCT, the concentrations of humic acid prepared were 0.01, 0.03, 0.06, 0.12, 0.17, 0.23, and 0.29 mg/L. Then we conducted experiments using a pilot plant with in-field of the serially connected bio-activated carbon filters and hollow fiber membrane processes employed in traditional water purification plants. Results showed under low TOC conditions of humic acid in influent (0.69 to 1.03 mg TOC/L) with an EBCT of 30 min, 40 min, and 50 min, TOC removal rates increases with greater EBCT, attaining about 39 % removal rate. The removal rate of THMs and HAAs by BACF was 54.8 % and 89.0 %, respectively.
Abstract: In this paper we introduce the notion of protein interaction network. This is a graph whose vertices are the protein-s amino acids and whose edges are the interactions between them. Using a graph theory approach, we observe that according to their structural roles, the nodes interact differently. By leading a community structure detection, we confirm this specific behavior and describe thecommunities composition to finally propose a new approach to fold a protein interaction network.
Abstract: Lectins have a good scope in current clinical
microbiology research. In the present study evaluated the
antimicrobial activities of a D-galactose binding lectin (PnL) was
purified from the annelid, Perinereis nuntia (polychaeta) by affinity
chromatography. The molecular mass of the lectin was determined to
be 32 kDa as a single polypeptide by SDS-PAGE under both reducing
and non-reducing conditions. The hemagglutinating activity of the
PnL showed against trypsinized and glutaraldehyde-fixed human
erythrocytes was specifically inhibited by D-Gal, GalNAc,
Galβ1-4Glc and Galα1-6Glc. PnL was evaluated for in vitro
antibacterial screening studies against 11 gram-positive and
gram-negative microorganisms. From the screening results, it was
revealed that PnL exhibited significant antibacterial activity against
gram-positive bacteria. Bacillus megaterium showed the highest
growth inhibition by the lectin (250 μg/disc). However, PnL did not
inhibit the growth of gram-negative bacteria such as Vibrio cholerae
and Pseudomonas sp. PnL was also examined for in vitro antifungal
activity against six fungal phytopathogens. PnL (100 μg/mL) inhibited
the mycelial growth of Alternaria alternata (24.4%). These results
indicate that future findings of lectin applications obtained from
annelids may be of importance to life sciences.
Abstract: In this study, the ability of Aspergillus niger and
Penicillium simplicissimum to extract heavy metals from a spent
refinery catalyst was investigated. For the first step, a spent
processing catalyst from one of the oil refineries in Iran was
physically and chemically characterized. Aspergillus niger and
Penicillium simplicissimum were used to mobilize Al/Co/Mo/Ni from
hazardous spent catalysts. The fungi were adapted to the mixture of
metals at 100-800 mg L-1 with increments in concentration of 100 mg
L-1. Bioleaching experiments were carried out in batch cultures. To
investigate the production of organic acids in sucrose medium,
analyses of the culture medium by HPLC were performed at specific
time intervals after inoculation. The results obtained from Inductive
coupled plasma-optical emission spectrometry (ICP-OES) showed
that after the one-step bioleaching process using Aspergillus niger,
maximum removal efficiencies of 27%, 66%, 62% and 38% were
achieved for Al, Co, Mo and Ni, respectively. However, the highest
removal efficiencies using Penicillium simplicissimum were of 32%,
67%, 65% and 38% for Al, Co, Mo and Ni, respectively
Abstract: The direct discharge of palm oil mill effluent (POME) wastewater causes serious environmental pollution due to its high chemical oxygen demand (COD) and biochemical oxygen demand (BOD). Traditional ways for POME treatment have both economical and environmental disadvantages. In this study, a membrane anaerobic system (MAS) was used as an alternative, cost effective method for treating POME. Six steady states were attained as a part of a kinetic study that considered concentration ranges of 8,220 to 15,400 mg/l for mixed liquor suspended solids (MLSS) and 6,329 to 13,244 mg/l for mixed liquor volatile suspended solids (MLVSS). Kinetic equations from Monod, Contois and Chen & Hashimoto were employed to describe the kinetics of POME treatment at organic loading rates ranging from 2 to 13 kg COD/m3/d. throughout the experiment, the removal efficiency of COD was from 94.8 to 96.5% with hydraulic retention time, HRT from 400.6 to 5.7 days. The growth yield coefficient, Y was found to be 0.62gVSS/g COD the specific microorganism decay rate was 0.21 d-1 and the methane gas yield production rate was between 0.25 l/g COD/d and 0.58 l/g COD/d. Steady state influent COD concentrations increased from 18,302 mg/l in the first steady state to 43,500 mg/l in the sixth steady state. The minimum solids retention time, which was obtained from the three kinetic models ranged from 5 to 12.3 days. The k values were in the range of 0.35 – 0.519 g COD/ g VSS • d and values were between 0.26 and 0.379 d-1. The solids retention time (SRT) decreased from 800 days to 11.6 days. The complete treatment reduced the COD content to 2279 mg/l equivalent to a reduction of 94.8% reduction from the original.
Abstract: Abstraction of water from the dry river sand-beds is
well-known as an alternative source of water during dry seasons.
Internally, because of the form of sand particles, voids are created
which can store water in the riverbeds. Large rivers are rare in South
Africa. Many rivers are sand river types and without water during the
prolonged dry periods. South Africa has not taken full advantage of
water storage in sand as a solution to the growing water scarcity both
in urban and rural areas. The paper reviews the benefits of run-off
storage in sand reservoirs gained from other arid areas and need for
adoption in rural areas of South Africa as an alternative water supply
where it is probable.
Abstract: Electric vehicles are considered as technology which
can significantly reduce the problems related to road transport such
as increasing GHG emissions, air pollutions and energy import
dependency.
The core objective of this paper is to analyze the current energetic,
ecological and economic characteristics of different types of electric
vehicles.
The major conclusions of this analysis are: The high investments
cost are the major barrier for broad market breakthrough of battery
electric vehicles and fuel cell vehicles. For battery electric vehicles
also the limited driving range states a key obstacle. The analyzed
hybrids could in principle serve as a bridging technology. However,
due to their tank-to-wheel emissions they cannot state a proper
solution for urban areas.
Finally, the most important perception is that also battery electric
vehicles and fuel cell vehicles are environmentally benign solution if
the primary fuel source is renewable.
Abstract: Diatoms are an important group of aquatic ecosystems and diatom-based indices are increasingly becoming important tools for the assessment of ecological conditions in lotic systems. Although the studies are very limited about Turkish rivers, diatom indices were used for monitoring rivers in different basins. In the present study, we used OMNIDIA program for estimation of stream quality. Some indices have less sensitive (IDP, WAT, LOBO, GENRE, TID, CEE, PT), intermediate sensitivities (IDSE, DESCY, IPS, DI-CH, SLA, IDAP), the others higher sensitivities (SID, IBD, SHE, EPI-D). Among the investigated diatom communities, only a few taxa indicated alfa-mesosaprobity and polysaprobity. Most of the sites were characterized by a great relative contribution of eutraphent and tolerant ones as well as oligosaprobic and betamesosaprobic diatoms. In general, SID and IBD indices gave the best results. This study suggests that the structure of benthic diatom communities and diatom indices, especially SID, can be applied for monitoring rivers in Southern Turkey.
Abstract: The purpose of the study reported here was designing Information Dissemination System (IDS) based E-learning in agricultural of Iran. A questionnaire was developed to designing Information Dissemination System. The questionnaire was distributed to 96 extension agents who work for Management of Extension and Farming System of Khuzestan province of Iran. Data collected were analyzed using the Statistical Package for the Social Sciences (SPSS). Appropriate statistical procedures for description (frequencies, percent, means, and standard deviations) were used. In this study there was a significant relationship between the age , IT skill and knowledge, years of extension work, the extend of information seeking motivation, level of job satisfaction and level of education with use of information technology by extension agent. According to extension agents five factors were ranked respectively as five top essential items to designing Information Dissemination System (IDS) based E-learning in agricultural of Iran. These factors include: 1) Establish communication between farmers, coordinators (extension agents), agricultural experts, research centers, and community by information technology. 2) The communication between all should be mutual. 3) The information must be based farmers need. 4) Internet used as a facility to transfer the advanced agricultural information to the farming community. 5) Farmers can be illiterate and speak a local and they are not expected to use the system directly. Knowledge produced by the agricultural scientist must be transformed in to computer understandable presentation. To designing Information Dissemination System, electronic communication, in the agricultural society and rural areas must be developed. This communication must be mutual between all factors.
Abstract: The human body has a complex system of innate and adaptive mechanisms for combating infection. This article discusses the role and relative effectiveness of these mechanisms in relation to small pox and AIDS.
Abstract: The optimal bisection width of r-dimensional N×
· · ·× N grid is known to be Nr-1 when N is even, but when
N is odd, only approximate values are available. This paper
shows that the exact bisection width of grid is Nr
-1
N-1 when N is odd.
Abstract: Certain tRNA synthetases have developed highly accurate molecular machinery to discriminate their cognate amino acids. Those aaRSs achieve their goal via editing reaction in the Connective Polypeptide 1 (CP1). Recently mutagenesis studies have revealed the critical importance of residues in the CP1 domain for editing activity and X-ray structures have shown binding mode of noncognate amino acids in the editing domain. To pursue molecular mechanism for amino acid discrimination, molecular modeling studies were performed. Our results suggest that aaRS bind the noncognate amino acid more tightly than the cognate one. Finally, by comparing binding conformations of the amino acids in three systems, the amino acid binding mode was elucidated and a discrimination mechanism proposed. The results strongly reveal that the conserved threonines are responsible for amino acid discrimination. This is achieved through side chain interactions between T252 and T247/T248 as well as between those threonines and the incoming amino acids.
Abstract: Irradiated material is a typical example of a complex
system with nonlinear coupling between its elements. During
irradiation the radiation damage is developed and this development
has bifurcations and qualitatively different kinds of behavior.
The accumulation of primary defects in irradiated crystals is
considered in frame work of nonlinear evolution of complex system.
The thermo-concentration nonlinear feedback is carried out as a
mechanism of self-oscillation development.
It is shown that there are two ways of the defect density evolution
under stationary irradiation. The first is the accumulation of defects;
defect density monotonically grows and tends to its stationary state
for some system parameters. Another way that takes place for
opportune parameters is the development of self-oscillations of the
defect density.
The stationary state, its stability and type are found. The
bifurcation values of parameters (environment temperature, defect
generation rate, etc.) are obtained. The frequency of the selfoscillation
and the conditions of their development is found and
rated. It is shown that defect density, heat fluxes and temperature
during self-oscillations can reach much higher values than the
expected steady-state values. It can lead to a change of typical
operation and an accident, e.g. for nuclear equipment.
Abstract: Circle grid space filling plate is a flow conditioner with a fractal pattern and used to eliminate turbulence originating from pipe fittings in experimental fluid flow applications. In this paper, steady state, incompressible, swirling turbulent flow through circle grid space filling plate has been studied. The solution and the analysis were carried out using finite volume CFD solver FLUENT 6.2. Three turbulence models were used in the numerical investigation and their results were compared with the pressure drop correlation of BS EN ISO 5167-2:2003. The turbulence models investigated here are the standard k-ε, realizable k-ε, and the Reynolds Stress Model (RSM). The results showed that the RSM model gave the best agreement with the ISO pressure drop correlation. The effects of circle grids space filling plate thickness and Reynolds number on the flow characteristics have been investigated as well.
Abstract: There are multiple reasons to expect that detecting the
word order errors in a text will be a difficult problem, and detection
rates reported in the literature are in fact low. Although grammatical
rules constructed by computer linguists improve the performance of
grammar checker in word order diagnosis, the repairing task is still
very difficult. This paper presents an approach for repairing word
order errors in English text by reordering words in a sentence and
choosing the version that maximizes the number of trigram hits
according to a language model. The novelty of this method concerns
the use of an efficient confusion matrix technique for reordering the
words. The comparative advantage of this method is that works with
a large set of words, and avoids the laborious and costly process of
collecting word order errors for creating error patterns.