Abstract: Bacterial cellulose, a biopolysaccharide, is produced by the bacterium, Gluconacetobacter xylinus. Static batch fermentation for bacterial cellulose production was studied in sucrose and date syrup solutions (Bx. 10%) at 28 °C using G. xylinus (PTCC, 1734). Results showed that the maximum yields of bacterial cellulose (BC) were 4.35 and 1.69 g/l00 ml for date syrup and sucrose medium after 336 hours fermentation period, respectively. Comparison of FTIR spectrum of cellulose with BC indicated appropriate coincidence which proved that the component produced by G. xylinus was cellulose. Determination of the area under X-ray diffractometry patterns demonstrated that the crystallinity amount of cellulose (83.61%) was more than that for the BC (60.73%). The scanning electron microscopy imaging of BC and cellulose were carried out in two magnifications of 1 and 6K. Results showed that the diameter ratio of BC to cellulose was approximately 1/30 which indicated more delicacy of BC fibers relative to cellulose.
Abstract: In this study, Li4SiO4 powder was successfully
synthesized via sol gel method followed by drying at 150oC. Lithium
oxide, Li2O and silicon oxide, SiO2 were used as the starting
materials with citric acid as the chelating agent. The obtained powder
was then sintered at various temperatures. Crystallographic phase
analysis, morphology and ionic conductivity were investigated
systematically employing X-ray diffraction, Fourier Transform
Infrared, Scanning Electron Microscopy and AC impedance
spectroscopy. XRD result showed the formation of pure monoclinic
Li4SiO4 crystal structure with lattice parameters a = 5.140 Å, b =
6.094 Å, c = 5.293 Å, β = 90o in the sample sintered at 750oC. This
observation was confirmed by FTIR analysis. The bulk conductivity
of this sample at room temperature was 3.35 × 10-6 S cm-1 and the
highest bulk conductivity of 1.16 × 10-4 S cm-1 was obtained at
100°C. The results indicated that, the Li4SiO4 compound has
potential to be used as host for LISICON structured solid electrolyte
for low temperature application.
Abstract: Power Spectral Density (PSD) of quasi-stationary processes can be efficiently estimated using the short time Fourier series (STFT). In this paper, an algorithm has been proposed that computes the PSD of quasi-stationary process efficiently using offline autoregressive model order estimation algorithm, recursive parameter estimation technique and modified sliding window discrete Fourier Transform algorithm. The main difference in this algorithm and STFT is that the sliding window (SW) and window for spectral estimation (WSA) are separately defined. WSA is updated and its PSD is computed only when change in statistics is detected in the SW. The computational complexity of the proposed algorithm is found to be lesser than that for standard STFT technique.
Abstract: Dispersions of casein micelles (CM) were studied at a
constant protein concentration of 5 wt % in high NaCl environment
ranging from 0% to 12% by Dynamic light scattering (DLS) and
Fourier Transform Infrared (FTIR). The rehydration profiles obtained
were interpreted in term of wetting, swelling and dispersion stages by
using a turbidity method. Two behaviours were observed depending
on the salt concentration. The first behaviour (low salt concentration)
presents a typical rehydration profile with a significant change
between 3 and 6% NaCl indicating quick wetting, swelling and long
dispersion stage. On the opposite, the dispersion stage of the second
behaviour (high salt concentration) was significantly shortened
indicating a strong modification of the protein backbone. A salt
increase result to a destabilization of the micelle and the formation of
mini-micelles more or less aggregated indicating an average micelles
size ranging from 100 to 200 nm. For the first time, the estimations
of secondary structural elements (irregular, ß-sheet, α-helix and turn)
by the Amide III assignments were correlated with results from
Amide I.
Abstract: Fractional Fourier Transform is a generalization of the classical Fourier Transform which is often symbolized as the rotation in time- frequency plane. Similar to the product of time and frequency span which provides the Uncertainty Principle for the classical Fourier domain, there has not been till date an Uncertainty Principle for the Fractional Fourier domain for a generalized class of finite energy signals. Though the lower bound for the product of time and Fractional Fourier span is derived for the real signals, a tighter lower bound for a general class of signals is of practical importance, especially for the analysis of signals containing chirps. We hence formulate a mathematical derivation that gives the lower bound of time and Fractional Fourier span product. The relation proves to be utmost importance in taking the Fractional Fourier Transform with adaptive time and Fractional span resolutions for a varied class of complex signals.
Abstract: Discovering new biological knowledge from the highthroughput biological data is a major challenge to bioinformatics today. To address this challenge, we developed a new approach for protein classification. Proteins that are evolutionarily- and thereby functionally- related are said to belong to the same classification. Identifying protein classification is of fundamental importance to document the diversity of the known protein universe. It also provides a means to determine the functional roles of newly discovered protein sequences. Our goal is to predict the functional classification of novel protein sequences based on a set of features extracted from each protein sequence. The proposed technique used datasets extracted from the Structural Classification of Proteins (SCOP) database. A set of spectral domain features based on Fast Fourier Transform (FFT) is used. The proposed classifier uses multilayer back propagation (MLBP) neural network for protein classification. The maximum classification accuracy is about 91% when applying the classifier to the full four levels of the SCOP database. However, it reaches a maximum of 96% when limiting the classification to the family level. The classification results reveal that spectral domain contains information that can be used for classification with high accuracy. In addition, the results emphasize that sequence similarity measures are of great importance especially at the family level.
Abstract: The use of magnetic and magnetic/gold core/shell
nanoparticles in biotechnology or medicine has shown good promise
due to their hybrid nature which possesses superior magnetic and
optical properties. Some of these potential applications include
hyperthermia treatment, bio-separations, diagnostics, drug delivery
and toxin removal. Synthesis refinement to control geometric and
magnetic/optical properties, and finding functional surfactants for
biomolecular attachment, are requirements to meet application
specifics.
Various high-temperature preparative methods were used for the
synthesis of iron oxide and gold-coated iron oxide nanoparticles.
Different surface functionalities, such as 11-aminoundecanoic and
11-mercaptoundecanoic acid, were introduced on the surface of the
particles to facilitate further attachment of biomolecular functionality
and drug-like molecules. Nanoparticle thermal stability, composition,
state of aggregation, size and morphology were investigated and the
results from techniques such as Fourier Transform-Infra Red
spectroscopy (FT-IR), Ultraviolet visible spectroscopy (UV-vis),
Transmission Electron Microscopy (TEM) and thermal analysis are
discussed.
Abstract: The zinc and iron environments in different growth
stages have been studied with EXAFS and XANES with Brookhaven
Synchrotron Light Source. Tissue samples included meat, organ,
vegetable, leaf, and yeast. The project studied the EXAFS and
XANES of tissue samples using Zn and Fe K-edges. Duck embryo
samples show that brain and intestine would contain shorter EXFAS
determined Zn-N/O bond; as with the cases of fresh yeast versus
reconstituted live yeast and green leaf versus yellow leaf. The
XANES Fourier transform characteristic-length would be useful as a
functionality index for selected types of tissue samples in various
physical states. The extension to the development of functional
synchrotron imaging for tissue engineering application based on
spectroscopic technique is discussed.
Abstract: Herein, we report the different types of surface morphology due to the interaction between the pure protein Insulin (INS) and catanionic surfactant mixture of Sodium Dodecyl Sulfate (SDS) and Cetyl Trimethyl Ammonium Bromide (CTAB) at air/water interface obtained by the Langmuir-Blodgett (LB) technique. We characterized the aggregations by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in LB films. We found that the INS adsorption increased in presence of catanionic surfactant at air/water interface. The presence of small amount of surfactant induces two-stage growth kinetics due to the pure protein absorption and protein-catanionic surface micelle interaction. The protein remains in native state in presence of small amount of surfactant mixture. Smaller amount of surfactant mixture with INS is producing surface micelle type structure. This may be considered for drug delivery system. On the other hand, INS becomes unfolded and fibrillated in presence of higher amount of surfactant mixture. In both the cases, the protein was successfully immobilized on a glass substrate by the LB technique. These results may find applications in the fundamental science of the physical chemistry of surfactant systems, as well as in the preparation of drug-delivery system.
Abstract: There are many kinds of metal borates found not only
in nature but also synthesized in the laboratory such as magnesium
borates. Due to its excellent properties, as remarkable ceramic
materials, they have also application areas in anti-wear and friction
reducing additives as well as electro-conductive treating agents. The
synthesis of magnesium borate powders can be fulfilled simply with
two different methods, hydrothermal and thermal synthesis.
Microwave assisted method, also another way of producing
magnesium borate, can be classified into thermal synthesis because of
using the principles of solid state synthesis. It also contributes
producing particles with small size and high purity in nano-size
material synthesize. In this study the production of magnesium
borates, are aimed using MgCl2.6H2O and H3BO3. The identification
of both starting materials and products were made by the equipments
of, X-Ray Diffraction (XRD) and Fourier Transform Infrared
Spectroscopy (FT-IR). After several synthesis steps magnesium
borates were synthesized and characterized by XRD and FT-IR, as
well.
Abstract: This research paper presents some methods to assess the performance of Wigner Ville Distribution for Time-Frequency representation of non-stationary signals, in comparison with the other representations like STFT, Spectrogram etc. The simultaneous timefrequency resolution of WVD is one of the important properties which makes it preferable for analysis and detection of linear FM and transient signals. There are two algorithms proposed here to assess the resolution and to compare the performance of signal detection. First method is based on the measurement of area under timefrequency plot; in case of a linear FM signal analysis. A second method is based on the instantaneous power calculation and is used in case of transient, non-stationary signals. The implementation is explained briefly for both methods with suitable diagrams. The accuracy of the measurements is validated to show the better performance of WVD representation in comparison with STFT and Spectrograms.
Abstract: Turkey has 72 % of total world boron reserves on the
basis of B2O3.Borates that is a refined form of boron minerals have a
wide range of applications. Zinc borates can be used as multifunctional
synergistic additives. The most important properties are
low solubility in water and high dehydration temperature. Zinc
borates dehydrate above 290°C and anhydrous zinc borate has
thermal resistance about 400°C. Zinc borates can be synthesized
using several methods such as hydrothermal and solid-state
processes. In this study, the solid-state method was applied between
500 and 800°C using the starting materials of ZnO and H3BO3 with
1:4 mole ratio. The reaction time was determined as 4 hours after
some preliminary experiments. After the synthesis, the crystal
structure and the morphology of the products were examined by XRay
Diffraction (XRD), Fourier Transform Infrared Spectroscopy
(FT-IR) and Raman Spectrometer. As a result the form of ZnB4O7
was synthesized with the highest crystal score at 800°C.
Abstract: The authors report a case of swine urolithiasis caused
by improper administration of sulfamonomethoxine and which was
diagnosed by examination of urinary sediments and analyzing the
composition of the uroliths. The chemical composition of urinary
calculi obtained from affected pigs with urolithiasis was further
confimed as sulfamonomethoxine by fourier transform infrared
(FTIR). It is suggested that appearance of typical fanlike or wheat
bunchy crystals in urinary sediments under observation of lightmicroscope
and determination by FTIR for the crystals are helpful in
diagnosing sulfa calculi causced swine urolithiasis.
Abstract: Enzymatic saccharification of biomass for reducing
sugar production is one of the crucial processes in biofuel production
through biochemical conversion. In this study, enzymatic
saccharification of dilute potassium hydroxide (KOH) pre-treated
Tetraselmis suecica biomass was carried out by using cellulase
enzyme obtained from Trichoderma longibrachiatum. Initially, the
pre-treatment conditions were optimised by changing alkali reagent
concentration, retention time for reaction, and temperature. The T.
suecica biomass after pre-treatment was also characterized using
Fourier Transform Infrared Spectra and Scanning Electron
Microscope. These analyses revealed that the functional group such
as acetyl and hydroxyl groups, structure and surface of T. suecica
biomass were changed through pre-treatment, which is favourable for
enzymatic saccharification process. Comparison of enzymatic
saccharification of untreated and pre-treated microalgal biomass
indicated that higher level of reducing sugar can be obtained from
pre-treated T. suecica. Enzymatic saccharification of pre-treated T.
suecica biomass was optimised by changing temperature, pH, and
enzyme concentration to solid ratio ([E]/[S]). Highest conversion of
carbohydrate into reducing sugar of 95% amounted to reducing sugar
yield of 20 (wt%) from pre-treated T. suecica was obtained from
saccharification, at temperature: 40°C, pH: 4.5 and [E]/[S] of 0.1
after 72 h of incubation. Hydrolysate obtained from enzymatic
saccharification of pretreated T. suecica biomass was further
fermented into biobutanol using Clostridium saccharoperbutyliticum
as biocatalyst. The results from this study demonstrate a positive
prospect of application of dilute alkaline pre-treatment to enhance
enzymatic saccharification and biobutanol production from
microalgal biomass.
Abstract: In 1990 [1] the subband-DFT (SB-DFT) technique was proposed. This technique used the Hadamard filters in the decomposition step to split the input sequence into low- and highpass sequences. In the next step, either two DFTs are needed on both bands to compute the full-band DFT or one DFT on one of the two bands to compute an approximate DFT. A combination network with correction factors was to be applied after the DFTs. Another approach was proposed in 1997 [2] for using a special discrete wavelet transform (DWT) to compute the discrete Fourier transform (DFT). In the first step of the algorithm, the input sequence is decomposed in a similar manner to the SB-DFT into two sequences using wavelet decomposition with Haar filters. The second step is to perform DFTs on both bands to obtain the full-band DFT or to obtain a fast approximate DFT by implementing pruning at both input and output sides. In this paper, the wavelet-based DFT (W-DFT) with Haar filters is interpreted as SB-DFT with Hadamard filters. The only difference is in a constant factor in the combination network. This result is very important to complete the analysis of the W-DFT, since all the results concerning the accuracy and approximation errors in the SB-DFT are applicable. An application example in spectral analysis is given for both SB-DFT and W-DFT (with different filters). The adaptive capability of the SB-DFT is included in the W-DFT algorithm to select the band of most energy as the band to be computed. Finally, the W-DFT is extended to the two-dimensional case. An application in image transformation is given using two different types of wavelet filters.
Abstract: Borate minerals have attracted considerable attention in the past years due to their structural chemistry and mechanical properties in several industries. Recently, increasing attention has been paid to the use of; synthetically produced magnesium borates as catalysts reinforcing material for plastics, the conversion of hydrocarbons, electro-conductive treating agent, anti-wear and anti-corrosion materials. Magnesium borates can be synthesized by several methods such as; hydrothermal and solid-state (thermal) processes. In this study the hydrothermal production method was applied at the modest temperature of 80C along with convenient crystal growth. Using MgCl2.6H2O, H3BO3, and NaOH as starting materials, 30, 60, 120, 240 minutes of reaction times were studied. After all, the crystal structure and the morphology of the products were examined by X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR). As a result the forms of Admontite and Mcallisterite minerals were synthesized.
Abstract: Zinc borates can be used as multi-functional
synergistic additives with flame retardant additives in polymers. Zinc
borate is white, non-hygroscopic and powder type product. The most
important properties are low solubility in water and high dehydration
temperature. Zinc borates dehydrate above 290°C and anhydrous zinc
borate has thermal resistance about 400°C. Zinc borates can be
synthesized using several methods such as hydrothermal and solidstate
processes. In this study, the solid-state method was applied at
low temperatures of 600oC and 700oC using the starting materials of
ZnO and H3BO3 with several mole ratios. The reaction time was
determined as 4 hours after some preliminary experiments. After the
synthesis, the crystal structure and the morphology of the products
were examined by X-Ray Diffraction (XRD) and Fourier Transform
Infrared Spectroscopy (FT-IR). As a result the forms of ZnB4O7,
Zn3(BO3)2, ZnB2O4 were synthesized and obtained along with the
unreacted ZnO.
Abstract: The complex structure of lignocellulose leads to great
difficulties in converting it to fermentable sugars for the ethanol
production. The major hydrolysis impediments are the crystallinity of
cellulose and the lignin content. To improve the efficiency of
enzymatic hydrolysis, microbial pretreatment of corncob was
investigated using two bacterial strains of Bacillus subtilis A 002 and
Cellulomonas sp. TISTR 784 (expected to break open the crystalline
part of cellulose) and lignin-degrading fungus, Phanerochaete
sordida SK7 (expected to remove lignin from lignocellulose). The
microbial pretreatment was carried out with each strain under its
optimum conditions. The pretreated corncob samples were further
hydrolyzed to produce reducing glucose with low amounts of
commercial cellulase (25 U·g-1 corncob) from Aspergillus niger. The
corncob samples were determined for composition change by X-ray
diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
and scanning electron microscope (SEM). According to the results,
the microbial pretreatment with fungus, P. sordida SK7 was the most
effective for enhancing enzymatic hydrolysis, approximately, 40%
improvement.
Abstract: The hybrid membranes containing inorganic materials in polymer matrix are identified as a remarkable family of proton conducting hybrid electrolytes. In this work, the proton conducting inorganic/organic hybrid membranes for proton exchange membrane fuel cells (PEMFCs) were prepared using polyvinyl alcohol (PVA), tetraethoxyorthosilane (TEOS) and heteropolyacid (HPA). The synthesized hybrid membranes were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), Scanning electron microscopy (SEM) and Thermogravimetry analysis (TGA). The effects of heteropolyacid incorporation on membrane properties, including morphology and thermal stability were extensively investigated.
Abstract: Power Spectral Density (PSD) computed by taking the Fourier transform of auto-correlation functions (Wiener-Khintchine Theorem) gives better result, in case of noisy data, as compared to the Periodogram approach. However, the computational complexity of Wiener-Khintchine approach is more than that of the Periodogram approach. For the computation of short time Fourier transform (STFT), this problem becomes even more prominent where computation of PSD is required after every shift in the window under analysis. In this paper, recursive version of the Wiener-Khintchine theorem has been derived by using the sliding DFT approach meant for computation of STFT. The computational complexity of the proposed recursive Wiener-Khintchine algorithm, for a window size of N, is O(N).