Abstract: In the paper we presented the possibility of application
collagen gels with active principle-s from marine algae extract and chlorhexidine salt in dental medicine. The hydro-alcoholic extracts
from marine algae have been used as they have been obtained. The extracts from marine algae and chlorhexidine salt (digluconate) are
incorporated in type I non-denatured fibrillar collagen matrixes. In order to obtain therapeutic effects at nanostructure level, it is important to know the rheological characteristics of the relevant mixtures of collagen gels and extracts from marine algae selected for use. In this survey we have studied mixtures made of non-denatured
fibrillar collagen hydro-gels where different concentrations of marine
algae have been incorporated. Based on the data obtained for the
shearing tensions, we have traced the rheograms – the diagrams for shearing tensions depending on the shearing speed values – from
which we have calculated the apparent viscosities as ratios between shearing tension and speed values, which have been figured in
relation to the shearing speed values, with a view to levelling
dependency.
Abstract: Enzymatic hydrolysis of starch from natural sources
finds potential application in commercial production of alcoholic
beverage and bioethanol. In this study the effect of starch
concentration, temperature, time and enzyme concentration were
studied and optimized for hydrolysis of cassava (Manihot esculenta)
starch powder (of mesh 80/120) into glucose syrup by immobilized
(using Polyacrylamide gel) a-amylase using central composite
design. The experimental result on enzymatic hydrolysis of cassava
starch was subjected to multiple linear regression analysis using
MINITAB 14 software. Positive linear effect of starch concentration,
enzyme concentration and time was observed on hydrolysis of
cassava starch by a-amylase. The statistical significance of the model
was validated by F-test for analysis of variance (p < 0.01). The
optimum value of starch concentration temperature, time and enzyme
concentration were found to be 4.5% (w/v), 45oC, 150 min, and 1%
(w/v) enzyme. The maximum glucose yield at optimum condition
was 5.17 mg/mL.
Abstract: According to FDA (Food and Drug Administration of the United States), vinegar is definedas a sour liquid containing at least 4 grams acetic acid in 100 cubic centimeter (4% solution of acetic acid) of solution that is produced from sugary materials by alcoholic fermentation. In the base of microbial starters, vinegars could be contained of more than 50 types of volatile and aromatic substances that responsible for their sweet taste and smelling. Recently the vinegar industry has a great proportion in agriculture, food and microbial biotechnology. The acetic acid bacteria are from the family Acetobacteraceae. Regarding to the latest version of Bergy-s Mannual of Systematic Bacteriology that has categorized bacteria in the base of their 16s RNA differences, the most important acetic acid genera are included Acetobacter (genus I), Gluconacetobacter (genus VIII) and Gluconobacter (genus IX). The genus Acetobacter that is primarily used in vinegar manufacturing plants is a gram negative, obligate aerobe coccus or rod shaped bacterium with the size 0.6 - 0.8 X 1.0 - 4.0 μm, nonmotile or motile with peritrichous flagella and catalase positive – oxidase negative biochemically. Some strains are overoxidizer that could convert acetic acid to carbon dioxide and water.In this research one Acetobacter native strain with high acetic acid productivity was isolated from Iranian white – red cherry. We used two specific culture media include Carr medium [yeast extract, 3%; ethanol, 2% (v/v); bromocresol green, 0.002%; agar, 2% and distilled water, 1000 ml], Frateur medium [yeast extract, 10 g/l; CaCO3, 20 g/l; ethanol, 20 g/l; agar, 20 g/l and distilled water, 1000 ml] and an industrial culture medium. In addition to high acetic acid production and high growth rate, this strain had a good tolerance against ethanol concentration that was examined using modified Carr media with 5%, 7% and 9% ethanol concentrations. While the industrial strains of acetic acid bacteria grow in the thermal range of 28 – 30 °C, this strain was adapted for growth in 34 – 36 °C after 96 hours incubation period. These dramatic characteristics suggest a potential biotechnological strain in production of cherry vinegar with a sweet smell and different nutritional properties in comparison to recent vinegar types. The lack of growth after 24, 48 and 72 hours incubation at 34 – 36 °C and the growth after 96 hours indicates a good and fast thermal flexibility of this strain as a significant characteristic of biotechnological and industrial strains.
Abstract: The increasing industrialization and motorization of the world has led to a steep rise for the demand of petroleum-based fuels. Petroleum-based fuels are obtained from limited reserves. These finite reserves are highly concentrated in certain regions of the world. Therefore, those countries not having these resources are facing energy/foreign exchange crisis, mainly due to the import of crude petroleum. Hence, it is necessary to look for alternative fuels which can be produced from resources available locally within the country such as alcohol, biodiesel, vegetable oils etc. Biodiesel is a renewable, domestically produced fuel that has been shown to reduce particulate, hydrocarbon, and carbon monoxide emissions from combustion. In the present study an experimental investigation on emission characteristic of a liquid burner system operating on several percentage of biodiesel and gas oil is carried out. Samples of exhaust gas are analysed with Testo 350 Xl. The results show that biodiesel can lower some pollutant such as CO, CO2 and particulate matter emissions while NOx emission would increase in comparison with gas oil. The results indicate there may be benefits to using biodiesel in industrial processes.
Abstract: Highly ordered TiO2 nanotube (TNT) arrays were
fabricated onto a pre-treated titanium foil by anodic oxidation with a
voltage of 20V in phosphoric acid/sodium fluoride electrolyte. A pretreatment
of titanium foil involved washing with acetone,
isopropanol, ethanol and deionized water. Carbon doped TiO2
nanotubes (C-TNT) was fabricated 'in-situ' with the same method in
the presence of polyvinyl alcohol and urea as carbon sources. The
affects of polyvinyl alcohol concentration and oxidation time on the
composition, morphology and structure of the C-TN were studied by
FE-SEM, EDX and XRD techniques. FESEM images of the
nanotubes showed uniform arrays of C-TNTs. The density and
microstructures of the nanotubes were greatly affected by the content
of PVA. The introduction of the polyvinyl alcohol into the electrolyte
increases the amount of C content inside TiO2 nanotube arrays
uniformly. The influence of carbon content on the photo-current of
C-TNT was investigated and the I-V profiles of the nanotubes were
established. The preliminary results indicated that the 'in-situ'
doping technique produced a superior quality nanotubes compared to
post doping techniques.
Abstract: A phorbol-12-myristate-13-acetate (TPA) is a synthetic analogue of phorbol ester (PE), a natural toxic compound of Euphorbiaceae plant. The oil extracted from plants of this family is useful source for primarily biofuel. However this oil might also be used as a foodstuff due to its significant nutrition content. The limitations for utilizing the oil as a foodstuff are mainly due to a toxicity of PE. Currently, a majority of PE detoxification processes are expensive as include multi steps alcohol extraction sequence.
Ozone is considered as a strong oxidative agent. It reacts with PE by attacking the carbon-carbon double bond of PE. This modification of PE molecular structure yields a non toxic ester with high lipid content.
This report presents data on development of simple and cheap PE detoxification process with water application as a buffer and ozone as reactive component. The core of this new technique is an application for a new microscale plasma unit to ozone production and the technology permits ozone injection to the water-TPA mixture in form of microbubbles.
The efficacy of a heterogeneous process depends on the diffusion coefficient which can be controlled by contact time and interfacial area. The low velocity of rising microbubbles and high surface to volume ratio allow efficient mass transfer to be achieved during the process. Direct injection of ozone is the most efficient way to process with such highly reactive and short lived chemical.
Data on the plasma unit behavior are presented and the influence of gas oscillation technology on the microbubble production mechanism has been discussed. Data on overall process efficacy for TPA degradation is shown.
Abstract: The purpose of this study was to analyze relationship
between gender, BMI, and lifestyle with bone mineral density
(BMD) of adolescent in urban areas . The place of this study in
Jakarta State University, Indonesia. The number of samples involved
as many as 200 people, consisting of 100 men and 100 women. BMD
was measured using Quantitative Ultrasound Bone Densitometry.
While the questionnaire used to collect data on age, gender, and
lifestyle (calcium intake, smoking habits, alcohol consumption, tea,
coffee, sports, and sun exposure). Mean age of men and women,
respectively as much as 20.7 ± 2.18 years and 21 ± 1.61 years. Mean
BMD values of men was 1.084 g/cm ² ± 0.11 while women was
0.976 g/cm ² ± 0.10. Men and women with normal BMD respectively
as much as 46.7% and 16.7%. Men and women affected by
osteopenia respectively as much as 50% and 80%. Men and women
affected by osteoporosis respectively as much as 3.3% and 3.3%.
Mean BMI of men and women, respectively as much as 21.4 ± 2.07
kg/m2 and 20.9 ± 2.06 kg/m2. Mean lifestyle score of men and
women , respectively as much as 71.9 ± 5.84 and 70.1 ± 5.67
(maximum score 100). Based on Spearman and Pearson Correlation
test, there were relationship significantly between gender and
lifestyle with BMD.
Abstract: A scaffold is necessary for tooth regeneration because of its three-dimensional geometry. For restoration of defect, it is necessary for the scaffold to be prepared in the shape of the defect. Sponges made from polyvinyl alcohol with formalin cross-linking (PVF sponge) have been used for scaffolds for bone formation in vivo. To induce osteogenesis within the sponge, methods of growing rat bone marrow cells (rBMCs) among the fiber structures in the sponge might be considered. Storage of rBMCs among the fibers in the sponge coated with dextran (10 kDa) was tried. After seeding of rBMCs to PVF sponge immersed in dextran solution at 2 g/dl concentration, osteogenesis was recognized in subcutaneously implanted PVF sponge as a scaffold in vivo. The level of osteocalcin was 25.28±5.71 ng/scaffold and that of Ca was 129.20±19.69 µg/scaffold. These values were significantly higher than those in sponges without dextran coating (p
Abstract: Arrack is one of the forms of alcoholic beverage or
liquor which is produced from palm or date juice and commonly
consumed by the lower social class of all religious/ethnic
communities in the north-western villages of Bangladesh. The
purpose of the study was to compare arrack drinking patterns
associated with socio-demographic status among the Muslim, Hindu,
Santal, and Oraon communities in the Rasulpur union of Bangladesh.
A total of 391 respondents (Muslim n-109, Hindu n-103, Santal n-89,
Oraon n-90) selected by cluster random sampling were interviewed
by ADP (Arrack Drinking Pattern) questionnaire. The results of
Pearson Chi-Squire test revealed that arrack drinking patterns were
significantly differed among the Muslim, Hindu, Santal, and Oraon
communities- drinkers. In addition, the results of Spearman-s
bivariate correlation coefficients also revealed that sociodemographic
characteristics of the communities- drinkers were the
significantly positive and negative associations with the arrack
drinking patterns in the Rasulpur union, Bangladesh. The study
suggests that further cross-cultural researches should be conducted
on the consequences of arrack drinking patterns on the communities-
drinkers.
Abstract: In this work Membrane Distillation is applied to
concentrate orange Juice. Clarified orange juice (11o Brix) obtained
from fresh fruits and a sugar solution was subjected to membrane
distillation. The experiments were performed on a flat sheet module
using orange juice and sucrose solution as feeds. The concentration
of a sucrose solution, used as a model fruit juice and also orange
juice, was carried out in a direct contact membrane distillation using
hydrophobic PTFE membrane of pore size 0.2 μm and porosity 70%.
Surface modification of PTFE membrane has been carried out by
treating membrane with alcohol and water solution to make it
hydrophilic and then hydrophobicity was regained by drying. The
influences of the feed temperature, feed concentration, flow rate,
operating time on the permeate flux were studied for treated and non
treated membrane. In this work treated and non treated membrane
were compared in terms of water flux, Within the tested range, MD
with surface modified membrane the water flux has been
significantly improved by treating the membrane surface.
Abstract: Polymeric microreactors have emerged as a new
generation of carriers that hold tremendous promise in the areas of
cancer therapy, controlled delivery of drugs, for removal of
pollutants etc. Present work reports a simple and convenient
methodology for synthesis of polystyrene and poly caprolactone
microreactors. An aqueous suspension of carboxylated (1μm)
polystyrene latex particles was mixed with toluene solution followed
by freezing with liquid nitrogen. Freezed particles were incubated at
-20°C and characterized for formation of voids on the surface of
polymer microspheres by Field Emission Scanning Electron
Microscope. The hollow particles were then overnight incubated at
40ºC with unfunctionalized quantum dots (QDs) in 5:1 ratio. QDs
Encapsulated polystyrene microcapsules were characterized by
fluorescence microscopy.
Likewise Poly ε-caprolactone microreactors were prepared by
micro-volcanic rupture of freeze dried microspheres synthesized
using emulsification of polymer with aqueous Poly vinyl alcohol and
freezed with liquid nitrogen. Microreactors were examined with Field
Emission Scanning Electron Microscope for size and morphology.
Current study is an attempt to create hollow polymer particles which
can be employed for microencapsulation of nanoparticles and drug
molecules.
Abstract: Lipases are enzymes particularly amenable for
immobilization by entrapment methods, as they can work equally
well in aqueous or non-conventional media and long-time stability of
enzyme activity and enantioselectivity is needed to elaborate more
efficient bioprocesses. The improvement of Pseudomonas
fluorescens (Amano AK) lipase characteristics was investigated by
optimizing the immobilization procedure in hybrid organic-inorganic
matrices using ionic liquids as additives. Ionic liquids containing a
more hydrophobic alkyl group in the cationic moiety are beneficial
for the activity of immobilized lipase. Silanes with alkyl- or aryl
nonhydrolizable groups used as precursors in combination with
tetramethoxysilane could generate composites with higher
enantioselectivity compared to the native enzyme in acylation
reactions of secondary alcohols. The optimal effect on both activity
and enantioselectivity was achieved for the composite made from
octyltrimethoxysilane and tetramethoxysilane at 1:1 molar ratio (60%
increase of total activity following immobilization and enantiomeric
ratio of 30). Ionic liquids also demonstrated valuable properties as
reaction media for the studied reactions, comparable with the usual
organic solvent, hexane.
Abstract: Aptamers are useful tools in microorganism
researches, diagnoses, and treatment. Aptamers are specific target
molecules formed by oligonucleic acid molecules, and are not
decomposed by alcohol. Aptamers used to detect Mycobacterium
tuberculosis (MTB) have been proved to have specific affinity to the
outer membrane proteins of MTB. This article presents a biosensor
chip set with aptamers for early detection of MTB with high specificity
and sensitivity, even in very low concentration. Meanwhile, we have
already made a modified hydrophobic facial mask module with
internal rendering hydrophobic for effectively collecting M.
tuberculosis.
Abstract: Experimental data are presented to show the influence of different types of chemical demulsifier on the stability and demulsification of emulsions. Three groups of demulsifier with different functional groups were used in this work namely amines, alcohol and polyhydric alcohol. The results obtained in this study have exposed the capability of chemical breaking agents in destabilization of water in crude oil emulsions. From the present study, found that molecular weight of the demulsifier were influent the capability of the emulsion to separate.
Abstract: Aligned and random nanofibrous scaffolds of PVA/PCL/nHA were fabricated by electrospinning method. The composite nanofibrous scaffolds were subjected to detailed analysis. Morphological investigations revealed that the prepared nanofibers have uniform morphology and the average fiber diameters of aligned and random scaffolds were 135.5 and 290 nm, respectively. The obtained scaffolds have a porous structure with porosity of 88 and 76% for random and aligned nanofibers, respectively. Furthermore, FTIR analysis demonstrated that there were strong intramolecular interactions between the molecules of PVA/PCL/nHA. On the other hand, mechanical characterizations show that aligning the nanofibers, could significantly improve the rigidity of the resultant biocomposite nanofibrous scaffolds.