Osteogenesis by Dextran Coating on and among Fibers of a Polyvinyl Formal Sponge
A scaffold is necessary for tooth regeneration because of its three-dimensional geometry. For restoration of defect, it is necessary for the scaffold to be prepared in the shape of the defect. Sponges made from polyvinyl alcohol with formalin cross-linking (PVF sponge) have been used for scaffolds for bone formation in vivo. To induce osteogenesis within the sponge, methods of growing rat bone marrow cells (rBMCs) among the fiber structures in the sponge might be considered. Storage of rBMCs among the fibers in the sponge coated with dextran (10 kDa) was tried. After seeding of rBMCs to PVF sponge immersed in dextran solution at 2 g/dl concentration, osteogenesis was recognized in subcutaneously implanted PVF sponge as a scaffold in vivo. The level of osteocalcin was 25.28±5.71 ng/scaffold and that of Ca was 129.20±19.69 µg/scaffold. These values were significantly higher than those in sponges without dextran coating (p<0.01). Osteogenesis was induced in many spaces in the inner structure of the sponge with dextran coated fibers.
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Kuboki, S. Saito, and Y. Mikuni-Takagaki, "Extracellular processing of
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[3] W. K. Jeong, S. H. Oh, J. H. Lee, and G. I. Im, "Repair of osteochondral
defects with a construct of mesenchymal stem cells and a
polydioxanone/poly (vinyl alcohol) scaffold," Biotechnol. Appl. Biochem.,
vol. 49, no. 2, pp. 155-164, Feb. 2008.
[4] C. R. Nuttelman, D. J. Mortisen, S. M. Henry, and K. S. Anseth,
"Attachment of fibronectin to poly(vinyl alcohol) hydrogels promotes
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[6] J. M. Liu, F. Haroun-Bouhedja, and C. Boisson-Vidal, "Analysis of the in
vitro inhibition of mammary adenocarcinoma cell adhesion by sulfated
polysaccharides," Anticancer Res., vol. 20, no. 5A, pp. 3265-3271,
Sep-Oct. 2000.
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by stromal cells obtained from bone marrow of young adult rats," Cell
Tissue Res., vol. 254, no. 2, 317-330, Nov. 1988.
[8] H. Ohgushi, Y. Dohi, T. Katuda, S. Tamai, S. Tabata, and Y. Suwa, "In
vitro bone formation by rat marrow cell culture," J. Biomed. Mater. Res.,
vol. 32, no. 3, pp. 333-340, Nov. 1996.
[9] D. Lemus, "Contributions of heterospecific tissue recombinations to
odontogenesis," Int. J. Dev. Biol., vol. 39, no. 1, pp. 291-297, Feb. 1995.
[10] J. Lewin-Epstein, B. Azaz, and M. Ulmansky, "Fate of osteogenic tissue
transferred to the subcutaneous area by means of polyvinyl-formal
sponge," Isr. J. Med. Sci., vol. 5, no. 3, pp. 365-372, May-Jun. 1969.
[11] E. Tsuruga, H. Takita, H. Itoh, Y. Wakisaka, and Y. Kuboki, "Pore size of
porous hydroxyapatite as the cell-substratum controls BMP-induced
osteogenesis," J. Biochem., vol. 121, no. 2, pp. 317-324, Feb. 1997.
[12] M. B. Zajaczkowski, E. Cukierman, C. G. Galbraith, and K. M. Yamada,
"Cell-matrix adhesions on poly (vinyl alcohol) hydrogels," Tissue Eng.,
vol. 9, no. 3, pp. 525-533, Jun. 2003.
[13] Y. W. Wang, Q. Wu, and G. Q. Chen, "Reduced mouse fibroblast cell
growth by increased hydrophilicity of microbial polyhydroxyalkanoates
via hyaluronan coating," Biomaterials, vol. 24, no. 25, pp. 4621-4629,
Nov. 2003.
[14] D. Li, K. Dai, and T. Tang, "Effects of dextran on proliferation and
osteogenic differentiation of human bone marrow-derived mesenchymal
stromal cells," Cytotherapy, vol. 10, no.6, pp. 587-596, Jun. 2008.
[15] M. G. Cascone, S. Maltinti, N. Barbani, and M. Laus, "Effect of chitosan
and dextran on the properties of poly (vinyl alcohol) hydrogels," J. Mater.
Sci. Mater. Med.., vol. 10, no.7, pp. 431-435, Jul. 1999.
[1] M. Satoyoshi, T. Koizumi, T. Teranaka, T. Iwamoto, H. Takita, Y.
Kuboki, S. Saito, and Y. Mikuni-Takagaki, "Extracellular processing of
dentin matrix protein in the mineralizing odontoblast culture," Calcif.
Tissue Int., vol. 57, no. 3, 237-241, Sep. 1995.
[2] P. Papagerakis, A. Berdal, M. Mesbah, M. Peuchmaur, L. Malaval, J.
Nydegger, J. Simmer, and M. Macdougall, "Investigation of osteocalcin,
osteonectin, and dentin sialophosphoprotein in developing human teeth,"
Bone, vol. 30, no. 2, pp. 377-385, Feb. 2002.
[3] W. K. Jeong, S. H. Oh, J. H. Lee, and G. I. Im, "Repair of osteochondral
defects with a construct of mesenchymal stem cells and a
polydioxanone/poly (vinyl alcohol) scaffold," Biotechnol. Appl. Biochem.,
vol. 49, no. 2, pp. 155-164, Feb. 2008.
[4] C. R. Nuttelman, D. J. Mortisen, S. M. Henry, and K. S. Anseth,
"Attachment of fibronectin to poly(vinyl alcohol) hydrogels promotes
NIH3T3 cell adhesion, proliferation, and migration," J. Biomed. Mater.
Res., vol. 57, no. 2, pp. 217-223, Nov. 2001.
[5] J. A. Cadée, M. J. van Luyn, L. A. Brouwer, J. A. Plantinga, P. B. van
Wachem, C. J. de Groot, W. den Otter, and W. E. Hennink, "In vivo
biocompatibility of dextran-based hydrogels," J. Biomed. Mater. Res., vol.
50, no. 3, pp. 397-404, Jun. 2000.
[6] J. M. Liu, F. Haroun-Bouhedja, and C. Boisson-Vidal, "Analysis of the in
vitro inhibition of mammary adenocarcinoma cell adhesion by sulfated
polysaccharides," Anticancer Res., vol. 20, no. 5A, pp. 3265-3271,
Sep-Oct. 2000.
[7] C. Maniatopoulos, J. Sodek, and A. H. Melcher, "Bone formation in vitro
by stromal cells obtained from bone marrow of young adult rats," Cell
Tissue Res., vol. 254, no. 2, 317-330, Nov. 1988.
[8] H. Ohgushi, Y. Dohi, T. Katuda, S. Tamai, S. Tabata, and Y. Suwa, "In
vitro bone formation by rat marrow cell culture," J. Biomed. Mater. Res.,
vol. 32, no. 3, pp. 333-340, Nov. 1996.
[9] D. Lemus, "Contributions of heterospecific tissue recombinations to
odontogenesis," Int. J. Dev. Biol., vol. 39, no. 1, pp. 291-297, Feb. 1995.
[10] J. Lewin-Epstein, B. Azaz, and M. Ulmansky, "Fate of osteogenic tissue
transferred to the subcutaneous area by means of polyvinyl-formal
sponge," Isr. J. Med. Sci., vol. 5, no. 3, pp. 365-372, May-Jun. 1969.
[11] E. Tsuruga, H. Takita, H. Itoh, Y. Wakisaka, and Y. Kuboki, "Pore size of
porous hydroxyapatite as the cell-substratum controls BMP-induced
osteogenesis," J. Biochem., vol. 121, no. 2, pp. 317-324, Feb. 1997.
[12] M. B. Zajaczkowski, E. Cukierman, C. G. Galbraith, and K. M. Yamada,
"Cell-matrix adhesions on poly (vinyl alcohol) hydrogels," Tissue Eng.,
vol. 9, no. 3, pp. 525-533, Jun. 2003.
[13] Y. W. Wang, Q. Wu, and G. Q. Chen, "Reduced mouse fibroblast cell
growth by increased hydrophilicity of microbial polyhydroxyalkanoates
via hyaluronan coating," Biomaterials, vol. 24, no. 25, pp. 4621-4629,
Nov. 2003.
[14] D. Li, K. Dai, and T. Tang, "Effects of dextran on proliferation and
osteogenic differentiation of human bone marrow-derived mesenchymal
stromal cells," Cytotherapy, vol. 10, no.6, pp. 587-596, Jun. 2008.
[15] M. G. Cascone, S. Maltinti, N. Barbani, and M. Laus, "Effect of chitosan
and dextran on the properties of poly (vinyl alcohol) hydrogels," J. Mater.
Sci. Mater. Med.., vol. 10, no.7, pp. 431-435, Jul. 1999.
@article{"International Journal of Medical, Medicine and Health Sciences:51290", author = "M. Yoshikawa and N. Tsuji and T. Yabuuchi and Y Shimomura and H. Kakigi and H. Hayashi and H. Ohgushi", title = "Osteogenesis by Dextran Coating on and among Fibers of a Polyvinyl Formal Sponge", abstract = "A scaffold is necessary for tooth regeneration because of its three-dimensional geometry. For restoration of defect, it is necessary for the scaffold to be prepared in the shape of the defect. Sponges made from polyvinyl alcohol with formalin cross-linking (PVF sponge) have been used for scaffolds for bone formation in vivo. To induce osteogenesis within the sponge, methods of growing rat bone marrow cells (rBMCs) among the fiber structures in the sponge might be considered. Storage of rBMCs among the fibers in the sponge coated with dextran (10 kDa) was tried. After seeding of rBMCs to PVF sponge immersed in dextran solution at 2 g/dl concentration, osteogenesis was recognized in subcutaneously implanted PVF sponge as a scaffold in vivo. The level of osteocalcin was 25.28±5.71 ng/scaffold and that of Ca was 129.20±19.69 µg/scaffold. These values were significantly higher than those in sponges without dextran coating (p", keywords = "Dextran, Polyvinyl formal sponge, Osteogenesis, Scaffold.", volume = "3", number = "6", pages = "58-5", }
