Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: Recently, genetic algorithms (GA) and particle swarm optimization (PSO) technique have attracted considerable attention among various modern heuristic optimization techniques. The GA has been popular in academia and the industry mainly because of its intuitiveness, ease of implementation, and the ability to effectively solve highly non-linear, mixed integer optimization problems that are typical of complex engineering systems. PSO technique is a relatively recent heuristic search method whose mechanics are inspired by the swarming or collaborative behavior of biological populations. In this paper both PSO and GA optimization are employed for finding stable reduced order models of single-input- single-output large-scale linear systems. Both the techniques guarantee stability of reduced order model if the original high order model is stable. PSO method is based on the minimization of the Integral Squared Error (ISE) between the transient responses of original higher order model and the reduced order model pertaining to a unit step input. Both the methods are illustrated through numerical example from literature and the results are compared with recently published conventional model reduction technique.
Abstract: This paper presents a compact thermoelectric power generator system based on temperature difference across the element. The system can transfer the burning heat energy to electric energy directly. The proposed system has a thermoelectric generator and a power control box. In the generator, there are 4 thermoelectric modules (TEMs), each of which uses 2 thermoelectric chips (TEs) and 2 cold sinks, 1 thermal absorber, and 1 thermal conduction flat board. In the power control box, there are 1 storing energy device, 1 converter, and 1 inverter. The total net generating power is about 11W. This system uses commercial portable gas stoves or burns timber or the coal as the heat source, which is easily obtained. It adopts solid-state thermoelectric chips as heat inverter parts. The system has the advantages of being light-weight, quite, and mobile, requiring no maintenance, and havng easily-supplied heat source. The system can be used a as long as burning is allowed. This system works well for highly-mobilized outdoors situations by providing a power for illumination, entertainment equipment or the wireless equipment at refuge. Under heavy storms such as typhoon, when the solar panels become ineffective and the wind-powered machines malfunction, the thermoelectric power generator can continue providing the vital power.
Abstract: The issue of unintentional islanding in PV grid
interconnection still remains as a challenge in grid-connected
photovoltaic (PV) systems. This paper discusses the overview of
popularly used anti-islanding detection methods, practically applied
in PV grid-connected systems. Anti-islanding methods generally can
be classified into four major groups, which include passive methods,
active methods, hybrid methods and communication base methods.
Active methods have been the preferred detection technique over the
years due to very small non-detected zone (NDZ) in small scale
distribution generation. Passive method is comparatively simpler
than active method in terms of circuitry and operations. However, it
suffers from large NDZ that significantly reduces its performance.
Communication base methods inherit the advantages of active and
passive methods with reduced drawbacks. Hybrid method which
evolved from the combination of both active and passive methods
has been proven to achieve accurate anti-islanding detection by many
researchers. For each of the studied anti-islanding methods, the
operation analysis is described while the advantages and
disadvantages are compared and discussed. It is difficult to pinpoint a
generic method for a specific application, because most of the
methods discussed are governed by the nature of application and
system dependent elements. This study concludes that the setup and
operation cost is the vital factor for anti-islanding method selection in
order to achieve minimal compromising between cost and system
quality.
Abstract: Blood pulse is an important human physiological signal commonly used for the understanding of the individual physical health. Current methods of non-invasive blood pulse sensing require direct contact or access to the human skin. As such, the performances of these devices tend to vary with time and are subjective to human body fluids (e.g. blood, perspiration and skin-oil) and environmental contaminants (e.g. mud, water, etc). This paper proposes a simulation model for the novel method of non-invasive acquisition of blood pulse using the disturbance created by blood flowing through a localized magnetic field. The simulation model geometry represents a blood vessel, a permanent magnet, a magnetic sensor, surrounding tissues and air in 2-dimensional. In this model, the velocity and pressure fields in the blood stream are described based on Navier-Stroke equations and the walls of the blood vessel are assumed to have no-slip condition. The blood assumes a parabolic profile considering a laminar flow for blood in major artery near the skin. And the inlet velocity follows a sinusoidal equation. This will allow the computational software to compute the interactions between the magnetic vector potential generated by the permanent magnet and the magnetic nanoparticles in the blood. These interactions are simulated based on Maxwell equations at the location where the magnetic sensor is placed. The simulated magnetic field at the sensor location is found to assume similar sinusoidal waveform characteristics as the inlet velocity of the blood. The amplitude of the simulated waveforms at the sensor location are compared with physical measurements on human subjects and found to be highly correlated.
Abstract: Sharing the manufacturing facility through remote
operation and monitoring of a machining process is challenge for
effective use the production facility. Several automation tools in term
of hardware and software are necessary for successfully remote
operation of a machine. This paper presents a prototype of workpiece
holding attachment for remote operation of milling process by self
configuration the workpiece setup. The prototype is designed with
mechanism to reorient the work surface into machining spindle
direction with high positioning accuracy. Variety of parts geometry
is hold by attachment to perform single setup machining. Pin type
with array pattern additionally clamps the workpiece surface from
two opposite directions for increasing the machining rigidity.
Optimum pins configuration for conforming the workpiece geometry
with minimum deformation is determined through hybrid algorithms,
Genetic Algorithms (GA) and Particle Swarm Optimization (PSO).
Prototype with intelligent optimization technique enables to hold
several variety of workpiece geometry which is suitable for
machining low of repetitive production in remote operation.
Abstract: Statistical learning theory was developed by Vapnik. It
is a learning theory based on Vapnik-Chervonenkis dimension. It also
has been used in learning models as good analytical tools. In general, a
learning theory has had several problems. Some of them are local
optima and over-fitting problems. As well, statistical learning theory
has same problems because the kernel type, kernel parameters, and
regularization constant C are determined subjectively by the art of
researchers. So, we propose an evolutionary statistical learning theory
to settle the problems of original statistical learning theory.
Combining evolutionary computing into statistical learning theory,
our theory is constructed. We verify improved performances of an
evolutionary statistical learning theory using data sets from KDD cup.
Abstract: An optical fiber Fabry-Perot interferometer (FFPI) is
proposed and demonstrated for dynamic measurements in a
mechanical vibrating target. A polishing metal with a low reflectance
value adhered to a mechanical vibrator was excited via a function
generator at various excitation frequencies. Output interference
fringes were generated by modulating the reference and sensing
signal at the output arm. A fringe-counting technique was used for
interpreting the displacement information on the dedicated computer.
The fiber interferometer has been found the capability of the
displacement measurements of 1.28 μm – 96.01 μm. A commercial
displacement sensor was employed as a reference sensor for
investigating the measurement errors from the fiber sensor. A
maximum percentage measurement error of approximately 1.59 %
was obtained.
Abstract: This paper reports a case study on how a conceptual
and analytical thinking approach was used in Art and Design Department at Multimedia University (Malaysia) in addressing the
issues of one nation and its impact in the society through artworks. The art project was designed for students to increase the know-how
and develop creative thinking in design and communication. Goals of the design project were: (1) to develop creative thinking in design
and communication, (2) to increase student understanding on the
process of problem solving for design work, and (3) to use design
elements and principles to generate interest, attention and emotional responses. An exhibition entitled "One Nation" was showcased to
local and international viewers consisting of the general public, professionals, academics, artists and students. Findings indicate that the project supported several visual art standards, as well as
generated awareness in the society. This project may be of interest to
current and future art educators and others interested in the potential
of utilizing global issues as content for art, community and environment studies for the purpose of educational art.
Abstract: The authors present an algorithm for order reduction of linear dynamic systems using the combined advantages of stability equation method and the error minimization by Genetic algorithm. The denominator of the reduced order model is obtained by the stability equation method and the numerator terms of the lower order transfer function are determined by minimizing the integral square error between the transient responses of original and reduced order models using Genetic algorithm. The reduction procedure is simple and computer oriented. It is shown that the algorithm has several advantages, e.g. the reduced order models retain the steady-state value and stability of the original system. The proposed algorithm has also been extended for the order reduction of linear multivariable systems. Two numerical examples are solved to illustrate the superiority of the algorithm over some existing ones including one example of multivariable system.
Abstract: The paper proposes an approach using genetic algorithm for computing the region based image similarity. The image is denoted using a set of segmented regions reflecting color and texture properties of an image. An image is associated with a family of image features corresponding to the regions. The resemblance of two images is then defined as the overall similarity between two families of image features, and quantified by a similarity measure, which integrates properties of all the regions in the images. A genetic algorithm is applied to decide the most plausible matching. The performance of the proposed method is illustrated using examples from an image database of general-purpose images, and is shown to produce good results.
Abstract: In this paper back-propagation artificial neural network
(BPANN) is employed to predict the deformation of the upsetting
process. To prepare a training set for BPANN, some finite element
simulations were carried out. The input data for the artificial neural
network are a set of parameters generated randomly (aspect ratio d/h,
material properties, temperature and coefficient of friction). The
output data are the coefficient of polynomial that fitted on barreling
curves. Neural network was trained using barreling curves generated
by finite element simulations of the upsetting and the corresponding
material parameters. This technique was tested for three different
specimens and can be successfully employed to predict the
deformation of the upsetting process
Abstract: Heterogeneity of solid waste characteristics as well as the complex processes taking place within the landfill ecosystem motivated the implementation of soft computing methodologies such as artificial neural networks (ANN), fuzzy logic (FL), and their combination. The present work uses a hybrid ANN-FL model that employs knowledge-based FL to describe the process qualitatively and implements the learning algorithm of ANN to optimize model parameters. The model was developed to simulate and predict the landfill gas production at a given time based on operational parameters. The experimental data used were compiled from lab-scale experiment that involved various operating scenarios. The developed model was validated and statistically analyzed using F-test, linear regression between actual and predicted data, and mean squared error measures. Overall, the simulated landfill gas production rates demonstrated reasonable agreement with actual data. The discussion focused on the effect of the size of training datasets and number of training epochs.
Abstract: In this paper we designed and implemented a new
ensemble of classifiers based on a sequence of classifiers which were
specialized in regions of the training dataset where errors of its
trained homologous are concentrated. In order to separate this
regions, and to determine the aptitude of each classifier to properly
respond to a new case, it was used another set of classifiers built
hierarchically. We explored a selection based variant to combine the
base classifiers. We validated this model with different base
classifiers using 37 training datasets. It was carried out a statistical
comparison of these models with the well known Bagging and
Boosting, obtaining significantly superior results with the
hierarchical ensemble using Multilayer Perceptron as base classifier.
Therefore, we demonstrated the efficacy of the proposed ensemble,
as well as its applicability to general problems.
Abstract: Abstract–Indoor air (VOCs) samples were collected
simultaneously from variety of indoors (e.g. living rooms, baby-s
rooms) and outdoor environments which were voluntarily selected
from the houses in which pregnant residents live throughout Ankara.
This is the first comprehensive study done in Turkey starting from
prenatal period and continued till the babies had one year old. VOCs
levels were measured over 76 homes. Air samples were collected in
Tenax TA sorbent filled tubes with active sampling method and
analyzed with Thermal Desorber and Gas Chromatography/Mass
spectrometry (TD-GC/MS). At the first sampling period in the baby-s
rooms maximum concentration of toluene was measured about
240.77μg.m-3 and in the living rooms maximum concentration of
naphthalene was 180.24μg.m-3. At the second sampling period in the
baby-s rooms maximum concentration of toluene was measured
about 144.97μg.m-3 and in the living rooms maximum concentration
of naphthalene was 247.89μg.m-3. Concentration of TVOCs in the
first period was generally higher than the second period.
Abstract: Under-representation of women in leadership positions" is still a general phenomenon in Germany despite the high number of implemented measures. The under-representation of female executives in the aviation sector is even worse. In this context our research hypothesis is that the representation and acceptance of women in management positions is determined by corporate culture.
Abstract: The aim of the present study is to analyze the generation of osteoporotic vertebral bone induced by lack of calcium during growth period and analyze its effects for disc degeneration, based on biomechanical and histomorphometrical study. Mechanical and histomorphological characteristics of lumbar vertebral bones and discs of rats with calcium free diet (CFD) were detected and tracked by using high resolution in-vivo micro-computed tomography (in-vivo micro-CT), finite element (FE) and histological analysis. Twenty female Sprague-Dawley rats (6 weeks old, approximate weight 170g) were randomly divided into two groups (CFD group: 10, NOR group: 10). The CFD group was maintained on a refmed calcium-controlled semisynthetic diet without added calcium, to induce osteoporosis. All lumbar (L 1-L6) were scanned by using in vivo micro-CT with 35i.un resolution at 0, 4, 8 weeks to track the effects of CFD on the generation of osteoporosis. The fmdings of the present study indicated that calcium insufficiency was the main factor in the generation of osteoporosis and it induced lumbar vertebral disc degeneration. This study is a valuable experiment to firstly evaluate osteoporotic vertebral bone and disc degeneration induced by lack of calcium during growth period from a biomechanical and histomorphometrical point of view.
Abstract: research goal was to determine the expression levels cDNA of brain embrio at gestation days 10 (GD-10). The Electroforesis DNA results showed that GAPDH, Fibronectin1, Ncam1, Tenascin, Vimentin, Neurofilament heavy, Neurofilament medium and Neurofilament low were 447 bp, 462 bp, 293 bp. 416 bp, 327 bp, 301 bp, 398 bp and 289 bp. Result of real-time RT-PCR on brain Embryo at gestation days 10 showed that the expression of copy gen Fibronectin 36 copies, Ncam 21,708 copies; Tenascin 24,505 copies; Vimentin 538,554 copies; Neurofilament heavy 2,419 copies; Neurofilament medium 92,928 copies; Neurofilament low 125,809 copies. Vimentin expressed gene copies is very high compared with other gene copies. This condition are caused by Vimentin, that contribute to proliferate of brain development. The vimentin role to cell proliferation of brain.
Abstract: In our current political climate of assessment and
accountability initiatives we are failing to prepare our children for a
participatory role in the creative economy. The field of education is
increasingly falling prey to didactic methodologies which train a
nation of competent test takers, foregoing the opportunity to educate
students to find problems and develop multiple solutions. No where is
this more evident than in the area of art education. Due to a myriad of
issues including budgetary shortfalls, time constraints and a general
misconception that anyone who enjoys the arts is capable of teaching
the arts, our students are not developing the skills they require to
become fully literate in critical thinking and creative processing.
Although art integrated curriculum is increasingly being viewed as a
reform strategy for motivating students by offering alternative
presentation of concepts and representation of knowledge acquisition,
misinformed administrators are often excluding the art teacher from
the integration equation. The paper to follow addresses the problem
of the need for divergent thinking and conceptualization in our
schools. Furthermore, this paper explores the role of education, and
specifically, art education in the development of a creatively literate
citizenry.
Abstract: Rapid economic development and population growth
in Malaysia had accelerated the generation of solid waste. This issue
gives pressure for effective management of municipal solid waste
(MSW) to take place in Malaysia due to the increased cost of landfill.
This paper discusses optimal planning of waste-to-energy (WTE)
using a combinatorial simulation and optimization model through
mixed integer linear programming (MILP) approach. The proposed
multi-period model is tested in Iskandar Malaysia (IM) as case study
for a period of 12 years (2011 -2025) to illustrate the economic
potential and tradeoffs involved in this study. In this paper, 3
scenarios have been used to demonstrate the applicability of the
model: (1) Incineration scenario (2) Landfill scenario (3) Optimal
scenario. The model revealed that the minimum cost of electricity
generation from 9,995,855 tonnes of MSW is estimated as USD
387million with a total electricity generation of 50MW /yr in the
optimal scenario.