Scholarly

Optimization of Breast Tumor Cells Isolation Efficiency and Purity by Membrane Filtration

Year: 2012 Volume: 6 Issue: 9 441 - 444 Pages

Abstract: Size based filtration is one of the common methods employed to isolate circulating tumor cells (CTCs) from whole blood. It is well known that this method suffers from isolation efficiency to purity tradeoff. However, this tradeoff is poorly understood. In this paper, we present the design and manufacturing of a special rectangular slit filter. The filter was designed to retain maximal amounts of nucleated cells, while minimizing the pressure on cells, thereby preserving their morphology. The key parameter, namely, input pressure, was optimized to retain the maximal number of tumor cells, whilst maximizing the depletion of normal blood cells (red and white blood cells and platelets). Our results indicate that for a slit geometry of 5 × 40 μm on a 13 mm circular membrane with a fill factor of 21%, a pressure of 6.9 mBar yields the optimum for maximizing isolation of MCF-7 and depletion of normal blood cells.

New SUZ-4 Zeolite Membrane from Sol-Gel Technique

Year: 2008 Volume: 2 Issue: 11 318 - 322 Pages

Abstract: A new SUZ-4 zeolite membrane with tetraethlyammonium hydroxide as the template was fabricated on mullite tube via hydrothermal sol-gel synthesis in a rotating autoclave reactor. The suitable synthesis condition was SiO2:Al2O3 ratio of 21.2 for 4 days at 155 °C crystallization under autogenous pressure. The obtained SUZ-4 possessed a high BET surface area of 396.4 m2/g, total pore volume at 2.611 cm3/g, and narrow pore size distribution with 97 nm mean diameter and 760 nm long of needle crystal shape. The SUZ-4 layer obtained from seeding crystallization was thicker than that of without seeds or in situ crystallization.

The Development of Flying Type Moving Robot Using Image Processing

Year: 2011 Volume: 5 Issue: 12 1808 - 1813 Pages

Abstract: Wheel-running type moving robot has the restriction on the moving range caused by obstacles or stairs. Solving this weakness, we studied the development of moving robot using airship. Our airship robot moves by recognizing arrow marks on the path. To have the airship robot recognize arrow marks, we used edge-based template matching. To control propeller units, we used PID and PD controller. The results of experiments demonstrated that the airship robot can move along the marks and can go up and down the stairs. It is shown the possibility that airship robot can become a robot which can move at wide range facilities.

An Algorithm for Secure Visible Logo Embedding and Removing in Compression Domain

Year: 2010 Volume: 4 Issue: 4 789 - 793 Pages

Abstract: Digital watermarking is the process of embedding information into a digital signal which can be used in DRM (digital rights managements) system. The visible watermark (often called logo) can indicate the owner of the copyright which can often be seen in the TV program and protects the copyright in an active way. However, most of the schemes do not consider the visible watermark removing process. To solve this problem, a visible watermarking scheme with embedding and removing process is proposed under the control of a secure template. The template generates different version of watermarks which can be seen visually the same for different users. Users with the right key can completely remove the watermark and recover the original image while the unauthorized user is prevented to remove the watermark. Experiment results show that our watermarking algorithm obtains a good visual quality and is hard to be removed by the illegally users. Additionally, the authorized users can completely remove the visible watermark and recover the original image with a good quality.

Multi-objective Optimisation of Composite Laminates under Heat and Moisture Effects using a Hybrid Neuro-GA Algorithm

Year: 2007 Volume: 1 Issue: 1 21 - 26 Pages

Abstract: In this paper, the optimum weight and cost of a laminated composite plate is seeked, while it undergoes the heaviest load prior to a complete failure. Various failure criteria are defined for such structures in the literature. In this work, the Tsai-Hill theory is used as the failure criterion. The theory of analysis was based on the Classical Lamination Theory (CLT). A newly type of Genetic Algorithm (GA) as an optimization technique with a direct use of real variables was employed. Yet, since the optimization via GAs is a long process, and the major time is consumed through the analysis, Radial Basis Function Neural Networks (RBFNN) was employed in predicting the output from the analysis. Thus, the process of optimization will be carried out through a hybrid neuro-GA environment, and the procedure will be carried out until a predicted optimum solution is achieved.

HPM Solution of Momentum Equation for Darcy-Brinkman Model in a Parallel Plates Channel Subjected to Lorentz Force

Year: 2011 Volume: 5 Issue: 1 164 - 168 Pages

Abstract: In this paper an analytical solution is presented for fully developed flow in a parallel plates channel under the action of Lorentz force, by use of Homotopy Perturbation Method (HPM). The analytical results are compared with exact solution and an excellent agreement has been observed between them for both Couette and Poiseuille flows. Moreover, the effects of key parameters have been studied on the dimensionless velocity profile.

Electrical Characteristics of Biomodified Electrodes using Nonfaradaic Electrochemical Impedance Spectroscopy

Year: 2011 Volume: 5 Issue: 1 70 - 74 Pages

Abstract: We demonstrate a nonfaradaic electrochemical impedance spectroscopy measurement of biochemically modified gold plated electrodes using a two-electrode system. The absence of any redox indicator in the impedance measurements provide more precise and accurate characterization of the measured bioanalyte at molecular resolution. An equivalent electrical circuit of the electrodeelectrolyte interface was deduced from the observed impedance data of saline solution at low and high concentrations. The detection of biomolecular interactions was fundamentally correlated to electrical double-layer variation at modified interface. The investigations were done using 20mer deoxyribonucleic acid (DNA) strands without any label. Surface modification was performed by creating mixed monolayer of the thiol-modified single-stranded DNA and a spacer thiol (mercaptohexanol) by a two-step self-assembly method. The results clearly distinguish between the noncomplementary and complementary hybridization of DNA, at low frequency region below several hundreds Hertz.

Influence of Turbulence Model, Grid Resolution and Free-Stream Turbulence Intensity on the Numerical Simulation of the Flow Field around an Inclined Flat Plate

Year: 2012 Volume: 6 Issue: 4 832 - 837 Pages

Abstract: The flow field around a flat plate of infinite span has been investigated for several values of the angle of attack. Numerical predictions have been compared to experimental measurements, in order to examine the effect of turbulence model and grid resolution on the resultant aerodynamic forces acting on the plate. Also the influence of the free-stream turbulence intensity, at the entrance of the computational domain, has been investigated. A full campaign of simulations has been conducted for three inclination angles (9°, 15° and 30°), in order to obtain some practical guidelines to be used for the simulation of the flow field around inclined plates and discs.

Keywords:
CFD
lift
drag
flat plate

Numbers and Biomass of Bacteria and Fungi Obtained by the Direct Microscopic Count Method

Year: 2012 Volume: 6 Issue: 12 1119 - 1123 Pages

Abstract: The soil ecology of the organic and mineral soil layers of laurel-leaved and Cryptomeria japonica forest in the Kasuga-yama Hill Primeval Forest (Nara, Japan) was assessed. The number of bacteria obtained by the dilution plate count method was less than 0.05% of those counted by the direct microscopic count. We therefore found that forest soil contains large numbers of non-culturable bacteria compared with agricultural soils. The numbers of bacteria and fungi obtained by both the dilution plate count and the direct microscopic count were larger in the deeper horizons (F and H) of the organic layer than in the mineral soil layer. This suggests that active microbial metabolism takes place in the organic layer. The numbers of bacteria and the length of fungal hyphae obtained by the direct count method were greater in the H horizon than in the F horizon. The direct microscopic count revealed numerous non-culturable bacteria and fungi in the soil. The ratio of fungal to bacterial biomass was lower in the laurel-leaved forest soil. The fungal biomass was therefore relatively low in the laurel-leaved forest soil due to differences in forest vegetation.

Design and Characteristics of New Test Facility for Flat Plate Boundary Layer Research

Year: 2009 Volume: 3 Issue: 10 1307 - 1313 Pages

Abstract: Preliminary results for a new flat plate test facility are presented here in the form of Computational Fluid Dynamics (CFD), flow visualisation, pressure measurements and thermal anemometry. The results from the CFD and flow visualisation show the effectiveness of the plate design, with the trailing edge flap anchoring the stagnation point on the working surface and reducing the extent of the leading edge separation. The flow visualization technique demonstrates the two-dimensionality of the flow in the location where the thermal anemometry measurements are obtained. Measurements of the boundary layer mean velocity profiles compare favourably with the Blasius solution, thereby allowing for comparison of future measurements with the wealth of data available on zero pressure gradient Blasius flows. Results for the skin friction, boundary layer thickness, frictional velocity and wall shear stress are shown to agree well with the Blasius theory, with a maximum experimental deviation from theory of 5%. Two turbulence generating grids have been designed and characterized and it is shown that the turbulence decay downstream of both grids agrees with established correlations. It is also demonstrated that there is little dependence of turbulence on the freestream velocity.

Hazardous Waste Management of Transmission Line Tower Manufacturing

Year: 2010 Volume: 4 Issue: 12 662 - 665 Pages

Abstract: The manufacturing transmission line tower parts has being generated hazardous waste which is required proper disposal of waste for protection of land pollution. Manufacturing Process in the manufacturing of steel angle, plates, pipes, channels are passes through conventional, semi automatic and CNC machines for cutting, marking, punching, drilling, notching, bending operations. All fabricated material Coated with thin layer of Zinc in Galvanizing plant where molten zinc is used for coating. Prior to Galvanizing, chemical like 33% concentrated HCl Acid, ammonium chloride and d-oil being used for pretreatment of iron. The bath of water with sodium dichromate is used for cooling and protection of the galvanized steel. For the heating purpose the furnace oil burners are used. These above process the Zinc dross, Zinc ash, ETP sludge and waste pickled acid generated as hazardous waste. The RPG has made captive secured land fill site, since 1997 since then it was using for disposal of hazardous waste after completion of SLF (Secured land fill) site. The RPG has raised height from ground level then now it is being used for disposal of waste as he designed the SLF after in creasing height of from GL it is functional without leach ate or adverse impacts in the environment.

A New Approach for Counting Passersby Utilizing Space-Time Images

Year: 2012 Volume: 6 Issue: 3 368 - 375 Pages

Abstract: Understanding the number of people and the flow of the persons is useful for efficient promotion of the institution managements and company-s sales improvements. This paper introduces an automated method for counting passerby using virtualvertical measurement lines. The process of recognizing a passerby is carried out using an image sequence obtained from the USB camera. Space-time image is representing the human regions which are treated using the segmentation process. To handle the problem of mismatching, different color space are used to perform the template matching which chose automatically the best matching to determine passerby direction and speed. A relation between passerby speed and the human-pixel area is used to distinguish one or two passersby. In the experiment, the camera is fixed at the entrance door of the hall in a side viewing position. Finally, experimental results verify the effectiveness of the presented method by correctly detecting and successfully counting them in order to direction with accuracy of 97%.

Numerical Study of Vertical Wall Jets: Influence of the Prandtl Number

Year: 2009 Volume: 3 Issue: 3 311 - 318 Pages

Abstract: This paper is a numerical investigation of a laminar isothermal plane two dimensional wall jet. Special attention has been paid to the effect of the inlet conditions at the nozzle exit on the hydrodynamic and thermal characteristics of the flow. The behaviour of various fluids evolving in both forced and mixed convection regimes near a vertical plate plane is carried out. The system of governing equations is solved with an implicit finite difference scheme. For numerical stability we use a staggered non uniform grid. The obtained results show that the effect of the Prandtl number is significant in the plume region in which the jet flow is governed by buoyant forces. Further for ascending X values, the buoyancy forces become dominating, and a certain agreement between the temperature profiles are observed, which shows that the velocity profile has no longer influence on the wall temperature evolution in this region. Fluids with low Prandtl number warm up more importantly, because for such fluids the effect of heat diffusion is higher.

Effects of Temperature-Dependent Material Properties on Stress and Temperature in Cracked Metal Plate under Electric Current Load

Year: 2010 Volume: 4 Issue: 5 448 - 453 Pages

Authors:
Thomas Jin-Chee Liu

Abstract: Using the finite element analyses, this paper discusses the effects of temperature-dependent material properties on the stress and temperature fields in a cracked metal plate under the electric current load. The practical and complicated results are obtained when the temperature-dependent material properties are adopted in the analysis. If the simplified (temperature-independent) material properties are used, incorrect results will be obtained.

Effects of Mold Surface Roughness on Compressible Flow of Micro-Injection Molding

Year: 2012 Volume: 6 Issue: 5 938 - 942 Pages

Abstract: Polymer melt compressibility and mold surface roughness, which are generally ignored during the filling stage of the conventional injection molding, may become increasingly significant in micro injection molding where the parts become smaller. By employing the 2.5D generalized Hele-Shaw model, we presented here the effects of polymer compressibility and mold surface roughness on mold-filling in a micro-thickness cavity. To elucidate the effects of surface roughness, numerical investigations were conducted using a cavity flat plate which has two halves with different surface roughness. This allows the comparison of flow field on two different halves under identical processing conditions but with different roughness. Results show that polymer compressibility and mold surface roughness have effects on mold filling in micro injection molding. There is in shrinkage reduction as the density is increased due to polymer melt compressibility during the filling stage.

Research on Applying the Continuity Care Document to Generate a Medical Record with Entry Level

Year: 2010 Volume: 4 Issue: 5 208 - 212 Pages

Abstract: Transferring patient information between medical care sites is necessary to deliver better patient care and to reduce medical cost. So developing of electronic medical records is an important trend for the world.The Continuity of Care Document (CCD) is product of collaboration between CDA and CCR standards. In this study, we will develop a system to generate medical records with entry level based on CCD template module.

Perfect Plastic Deformation of a Circular Thin Bronze Plate due to the Growth and Collapse of a Vapour Bubble

Year: 2012 Volume: 6 Issue: 1 203 - 208 Pages

Abstract: Dynamics of a vapour bubble generated due to a high local energy input near a circular thin bronze plate in the absence of the buoyancy forces is numerically investigated in this paper. The bubble is generated near a thin bronze plate and during the growth and collapse of the bubble, it deforms the nearby plate. The Boundary Integral Equation Method is employed for numerical simulation of the problem. The fluid is assumed to be incompressible, irrotational and inviscid and the surface tension on the bubble boundary is neglected. Therefore the fluid flow around the vapour bubble can be assumed as a potential flow. Furthermore, the thin bronze plate is assumed to have perfectly plastic behaviour. Results show that the displacement of the circular thin bronze plate has considerable effect on the dynamics of its nearby vapour bubble. It is found that by decreasing the thickness of the thin bronze plate, the growth and collapse rate of the bubble becomes higher and consequently the lifetime of the bubble becomes shorter.

BEM Formulations Based on Kirchhoffs Hypoyhesis to Perform Linear Bending Analysis of Plates Reinforced by Beams

Year: 2007 Volume: 1 Issue: 2 84 - 89 Pages

Abstract: In this work, are discussed two formulations of the boundary element method - BEM to perform linear bending analysis of plates reinforced by beams. Both formulations are based on the Kirchhoff's hypothesis and they are obtained from the reciprocity theorem applied to zoned plates, where each sub-region defines a beam or a slab. In the first model the problem values are defined along the interfaces and the external boundary. Then, in order to reduce the number of degrees of freedom kinematics hypothesis are assumed along the beam cross section, leading to a second formulation where the collocation points are defined along the beam skeleton, instead of being placed on interfaces. On these formulations no approximation of the generalized forces along the interface is required. Moreover, compatibility and equilibrium conditions along the interface are automatically imposed by the integral equation. Thus, these formulations require less approximation and the total number of the degree s of freedom is reduced. In the numerical examples are discussed the differences between these two BEM formulations, comparing as well the results to a well-known finite element code.

Numerical Analysis of Wind Loads on a Hemicylindrical Roof Building

Year: 2011 Volume: 5 Issue: 8 1602 - 1609 Pages

Abstract: The flow field over a three dimensional pole barn characterized by a cylindrical roof has been numerically investigated. Wind pressure and viscous loads acting on the agricultural building have been analyzed for several incoming wind directions, so as to evaluate the most critical load condition on the structure. A constant wind velocity profile, based on the maximum reference wind speed in the building site (peak gust speed worked out for 50 years return period) and on the local roughness coefficient, has been simulated. In order to contemplate also the hazard due to potential air wedging between the stored hay and the lower part of the ceiling, the effect of a partial filling of the barn has been investigated. The distribution of wind-induced loads on the structure have been determined, allowing a numerical quantification of the effect of wind direction on the induced stresses acting on a hemicylindrical roof.

Development of an Infrared Thermography Method with CO2 Laser Excitation, Applied to Defect Detection in CFRP

Year: 2013 Volume: 7 Issue: 8 589 - 593 Pages

Abstract: This paper presents a NDT by infrared thermography with excitation CO2 Laser, wavelength of 10.6 μm. This excitation is the controllable heating beam, confirmed by a preliminary test on a wooden plate 1.2 m x 0.9 m x 1 cm. As the first practice, this method is applied to detecting the defect in CFRP heated by the Laser 300 W during 40 s. Two samples 40 cm x 40 cm x 4.5 cm are prepared, one with defect, another one without defect. The laser beam passes through the lens of a deviation device, and heats the samples placed at a determinate position and area. As a result, the absence of adhesive can be detected. This method displays prominently its application as NDT with the composite materials. This work gives a good perspective to characterize the laser beam, which is very useful for the next detection campaigns.

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