Abstract: Microbes have been used to solve environmental
problems for many years. The role of microorganism to sequester,
precipitate or alter the oxidation state of various heavy metals has
been extensively studied. Treatment using microorganism interacts
with toxic metal are very diverse. The purpose of this research is to
remove the mercury using Pseudomonas putida (P. putida), pure
culture ATTC 49128 at optimum growth parameters such as
techniques of culture, acclimatization time and speed of incubator
shaker. Thus, in this study, the optimum growth parameters of P.
putida were obtained to achieve the maximum of mercury removal.
Based on the optimum parameters of P. putida for specific growth
rate, the removal of two different mercury concentration, 1 ppm and
4 ppm were studied. From mercury nitrate solution, a mercuryresistant
bacterial strain which is able to reduce from ionic mercury
to metallic mercury was used to reduce ionic mercury. The overall
levels of mercury removal in this study were between 80% and 89%.
The information obtained in this study is of fundamental for
understanding of the survival of P. putida ATTC 49128 in mercury
solution. Thus, microbial mercury removal is a potential
bioremediation for wastewater especially in petrochemical industries
in Malaysia.
Abstract: Toddy sediment (TS) was cultured in a PDA medium
to determine initial yeast load, and also it was undergone sun, shade,
solar, dehumidified cold air (DCA) and hot air oven (at 400, 500 and
60oC) drying with a view to preserve viability of yeast. Thereafter,
this study was conducted according to two factor factorial design in
order to determine best preservation method. Therein the dried TS
from the best drying method was taken and divided into two portions.
One portion was mixed with 3: 7 ratio of TS: rice flour and the
mixture was divided in to two again. While one portion was kept
under in house condition the other was in a refrigerator. Same
procedure was followed to the rest portion of TS too but it was at the
same ratio of corn flour. All treatments were vacuum packed in triple
laminate pouches and the best preservation method was determined
in terms of leavening index (LI). The TS obtained from the best
preservation method was used to make foods (bread and hopper) and
organoleptic properties of it were evaluated against same of ordinary
foods using sensory panel with a five point hedonic scale.
Results revealed that yeast load or fresh TS was 58×106 CFU/g.
The best drying method in preserving viability of yeast was DCA
because LI of this treatment (96%) is higher than that of other three
treatments. Organoleptic properties of foods prepared from best
preservation method are as same as ordinary foods according to Duo
trio test.
Abstract: A Japanese manga character, Doraemon, was made by
Fujiko F. Fujio in 1969, was made into animation in 1973. The main
character, Doraemon, is a robot cat, and is a well-known Japanese
animated character. However, Doraemon is not only regarded as an
animation character but it is also used in educational and technological
programs in Japan.
This paper focuses on the background of Doraemon, educational
and technological perspectives on Doraemon, and comparison of the
original Japanese animation and the US remade version, and the
animator Fujiko’s dreams and hopes for Doraemon will be examined.
Since Doraemon has been exported as animation and manga to
overseas, perspectives toward Doraemon have changed. For example,
changes of stories and characters can been seen in the present
Doraemon animation. Not only the overseas TV productions which
broadcast Doraemon but also the Japanese production has to consider
violence, sexuality, etc. when editing episodes. Because of
representation of cultural differences, Japanese animation is thought to
contain more violence, discrimination, and sexuality in animation.
With responses from overseas, the Japanese production was cautious
about the US remade version. They cared about the US Broadcast
Standard, and tried to consider US customs and culture in the US
remade version. Seeing the difference, acculturation is necessary for
exports of animation overseas. Moreover, observing different aspects
of Doraemon domestically, Doraemon provides dreams and hopes to
children.
Abstract: The iron environment in Fe-doped Vycor Anode was
investigated with EXAFS using Brookhaven Synchrotron Light
Source. The iron-reducing Shewanella oneidensis culture was grown
in a microbial fuel cell under anaerobic respiration. The Fe bond
length was found to decrease and correlate with the amount of
biofilm growth on the Fe-doped Vycor Anode. The data suggests that
Fe-doped Vycor Anode would be a good substrate to study the
Shewanella oneidensis nanowire structure using EXAFS.
Abstract: Historical narration is an act that necessarily develops
and deforms history. This “translation” is examined within the
historical and political context of the 1930 Berlin film premiere of
“All Quiet on the Western Front,” a film based on Erich Maria
Remarque’s 1928 best-selling novel. Both the film and the novel
appeared during an era in which life was conceived of as innately
artistic. The emergence of this “aestheticization” of memory and
history enabled conservative propaganda of the period to denounce
all art that did not adhere conceptually to its political tenets, with “All
Quiet” becoming yet another of its “victims.”
Abstract: Quick adoption of e-business and emerging influence
of “Electronic Word of Mouth e-WOM” communication on guests
made leading hotel brands successful examples of electronic guest
relationship management. Main reasons behind such success are well
established procedures in collection, analysis and usage of highly
valuable data available on the Internet, generated through some form
of e-GRM programme. E-GRM is more than just a technology
solution. It’s a system which balance respective guest demands, hotel
technological capabilities and organizational culture of employees,
discharging the universal approach in guest relations “same for all”.
The purpose of this research derives from the necessity of
determining the importance of monitoring and applying e-WOM
communication as one of the methods used in managing guest
relations. This paper analyses and compares different hotelier’s
opinions on e-WOM communication.
Abstract: The use of engineered nanomaterials has increased as
a result of their positive impact on many sectors of the economy,
including agriculture. Silver nanoparticles (AgNPs) are now used to
enhance seed germination, plant growth, and photosynthetic quantum
efficiency and as antimicrobial agents to control plant diseases. In
this study, we examined the effect of AgNP dosage on the seed
germination of three plant species: corn (Zea mays L.), watermelon
(Citrullus lanatus [Thunb.] Matsum. & Nakai) and zucchini
(Cucurbita pepo L.). This experiment was designed to study the
effect of AgNPs on germination percentage, germination rate, mean
germination time, root length and fresh and dry weight of seedlings
for the three species. Seven concentrations (0.05, 0.1, 0.5, 1, 1.5, 2
and 2.5 mg/ml) of AgNPs were examined at the seed germination
stage. The three species had different dose responses to AgNPs in
terms of germination parameters and the measured growth
characteristics. The germination rates of the three plants were
enhanced in response to AgNPs. Significant enhancement of the
germination percentage values was observed after treatment of the
watermelon and zucchini plants with AgNPs in comparison with
untreated seeds. AgNPs showed a toxic effect on corn root
elongation, whereas watermelon and zucchini seedling growth were
positively affected by certain concentrations of AgNPs. This study
showed that exposure to AgNPs caused both positive and negative
effects on plant growth and germination.
Abstract: This study investigates the use of a time-series of
MODIS NDVI data to identify agricultural land cover change on an
annual time step (2007 - 2012) and characterize the trend. Following
an ISODATA classification of the MODIS imagery to selectively
mask areas not agriculture or semi-natural, NDVI signatures were
created to identify areas cereals and vineyards with the aid of
ancillary, pictometry and field sample data for 2010. The NDVI
signature curve and training samples were used to create a decision
tree model in WEKA 3.6.9 using decision tree classifier (J48)
algorithm; Model 1 including ISODATA classification and Model 2
not. These two models were then used to classify all data for the
study area for 2010, producing land cover maps with classification
accuracies of 77% and 80% for Model 1 and 2 respectively. Model 2
was subsequently used to create land cover classification and change
detection maps for all other years. Subtle changes and areas of
consistency (unchanged) were observed in the agricultural classes
and crop practices. Over the years as predicted by the land cover
classification. Forty one percent of the catchment comprised of
cereals with 35% possibly following a crop rotation system.
Vineyards largely remained constant with only one percent
conversion to vineyard from other land cover classes.
Abstract: The largest share of policy and money within the
European Union goes to agriculture. The Union’s Common
Agricultural Policy has undergone several transformations in the last
five decades, with the main change taking place in the 1990s. This
change influenced agriculture in the Czech Republic, inasmuch as the
fledgling republic was preparing to join the European Union and
adopt its policies. In the 1990s, Czech agriculture passed from
a centrally planned economy to a market economy and subsequently
adopted the terms of the Common Agricultural Policy. The Czech
Republic is also characterized by a significant diversification of
landscape sphere. Agricultural entrepreneurs in the Czech Republic
are still not accustomed to the possibility of grants from the European
Union. They focus rather on national or regional subsidies. Only half
of all agricultural entrepreneurs in the Czech Republic use European
subsidies. This article focuses on the introduction of the Common
Agricultural Policy to the Czech Republic and its subsequent
influence on Czech agriculture. It is demonstrated through the
implementation rate of the CAP in the EU Member States and
a closer focus on Czech integration.
Abstract: This paper is part of a study to develop robots for
farming. As such power requirement to operate equipment attach to
such robots become an important factor. Soil-tool interaction plays
major role in power consumption, thus predicting accurately the
forces which act on the blade during the farming is very important for
optimal designing of farm equipment. In this paper, a finite element
investigation for tillage tools and soil interaction is described by
using an inelastic constitutive material law for agriculture
application. A 3-dimensional (3D) nonlinear finite element analysis
(FEA) is developed to examine behavior of a blade with different
rake angles moving in a block of soil, and to estimate the blade force.
The soil model considered is an elastic-plastic with non-associated
Drucker-Prager material model. Special use of contact elements are
employed to consider connection between soil-blade and soil-soil
surfaces. The FEA results are compared with experimental ones,
which show good agreement in accurately predicting draft forces
developed on the blade when it moves through the soil. Also a very
good correlation was obtained between FEA results and analytical
results from classical soil mechanics theories for straight blades.
These comparisons verified the FEA model developed. For analyzing
complicated soil-tool interactions and for optimum design of blades,
this method will be useful.
Abstract: A total of 115 yeast strains isolated from local cassava
processing wastes were measured for crude protein content. Among
these strains, the strain MSY-2 possessed the highest protein
concentration (>3.5 mg protein/mL). By using molecular
identification tools, it was identified to be a strain of Pichia
kudriavzevii based on similarity of D1/D2 domain of 26S rDNA
region. In this study, to optimize the protein production by MSY-2
strain, Response Surface Methodology (RSM) was applied. The
tested parameters were the carbon content, nitrogen content, and
incubation time. Here, the value of regression coefficient (R2) =
0.7194 could be explained by the model which is high to support the
significance of the model. Under the optimal condition, the protein
content was produced up to 3.77 g per L of the culture and MSY-2
strain contains 66.8 g protein per 100 g of cell dry weight. These
results revealed the plausibility of applying the novel strain of yeast
in single-cell protein production.
Abstract: Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
Abstract: The paper presents a new method for efficient
innovation process management. Even though the innovation
management methods, tools and knowledge are well established and
documented in literature, most of the companies still do not manage it
efficiently. Especially in SMEs the front end of innovation - problem
identification, idea creation and selection - is often not optimally
performed. Our eMIPS methodology represents a sort of "umbrella
methodology" - a well-defined set of procedures, which can be
dynamically adapted to the concrete case in a company. In daily
practice, various methods (e.g. for problem identification and idea
creation) can be applied, depending on the company's needs. It is
based on the proactive involvement of the company's employees
supported by the appropriate methodology and external experts. The
presented phases are performed via a mixture of face-to-face
activities (workshops) and online (eLearning) activities taking place
in eLearning Moodle environment and using other e-communication
channels. One part of the outcomes is an identified set of
opportunities and concrete solutions ready for implementation. The
other also very important result is connected to innovation
competences for the participating employees related with concrete
tools and methods for idea management. In addition, the employees
get a strong experience for dynamic, efficient and solution oriented
managing of the invention process. The eMIPS also represents a way
of establishing or improving the innovation culture in the
organization. The first results in a pilot company showed excellent
results regarding the motivation of participants and also as to the
results achieved.
Abstract: The intrinsic contradictions of entrepreneurship
development and self-development strategies complicate the task of
reaching compliance between the state economic policy and the
company entrepreneurship policy: on the one hand, there is a
contradiction between the social and the competitive order within
economic order policy and on the other hand, the contradiction exists
between entrepreneurship strategy and entrepreneurship culture
within entrepreneurship policy.
Abstract: Microalgae Meyerella planktonica is a potential
biofuel source because it can grow in bulk in either autotrophic or
heterotrophic condition. However, the quantitative growth of this
algal type is still low as it tends to precipitates on the bottom.
Besides, the lipid concentration is still low when grown in
autotrophic condition. In contrast, heterotrophic condition can
enhance the lipid concentration. The combination of autotrophic
condition and agitation treatment was conducted to increase the
density of the culture. On the other hand, a heterotrophic condition
was set up to raise the lipid production. A two-stage experiment
was applied to increase the density at the first step and to increase
the lipid concentration in the next step. The autotrophic condition
resulted higher density but lower lipid concentration compared to
heterotrophic one. The agitation treatment produced higher density
in both autotrophic and heterotrophic conditions. The two-stage
experiment managed to enhance the density during the autotrophic
stage and the lipid concentration during the heterotrophic stage.
The highest yield was performed by using 0.4% v/v glycerol as a
carbon source (2.9±0.016 x 10^6 cells w/w) attained 7 days after the
heterotrophic stage began. The lipid concentration was stable
starting from day 7.
Abstract: The introduction of degradable plastic materials into
agricultural sectors has represented a promising alternative to
promote green agriculture and environmental friendly of modern
farming practices. Major challenges of developing degradable
agricultural films are to identify the most feasible types of
degradation mechanisms, composition of degradable polymers and
related processing techniques. The incorrect choice of degradable
mechanisms to be applied during the degradation process will cause
premature losses of mechanical performance and strength. In order to
achieve controlled process of agricultural film degradation, the
compositions of degradable agricultural film also important in order
to stimulate degradation reaction at required interval of time and to
achieve sustainability of the modern agricultural practices. A set of
photodegradable polyethylene based agricultural film was developed
and produced, following the selective optimization of processing
parameters of the agricultural film manufacturing system. Example of
agricultural films application for oil palm seedlings cultivation is
presented.
Abstract: Phelipanche ramosa is the most damaging obligate
flowering parasitic weed on wide species of cultivated plants. The
semi-arid regions of the world are considered the main centers of this
parasitic plant that causes heavy infestation. This is due to its
production of high numbers of seeds (up to 200,000) that remain
viable for extended periods (up to 20 years). In this study, 13
treatments for the control of Phelipanche were carried out, which
included agronomic, chemical, and biological treatments and the use
of resistant plant methods. In 2014, a trial was performed at the
Department of Agriculture, Food and Environment, University of
Foggia (southern Italy), on processing tomato (cv ‘Docet’) grown in
pots filled with soil taken from a field that was heavily infested by P.
ramosa). The tomato seedlings were transplanted on May 8, 2014,
into a sandy-clay soil (USDA). A randomized block design with 3
replicates (pots) was adopted. During the growing cycle of the
tomato, at 70, 75, 81 and 88 days after transplantation, the number of
P. ramosa shoots emerged in each pot was determined. The tomato
fruit were harvested on August 8, 2014, and the quantitative and
qualitative parameters were determined. All of the data were
subjected to analysis of variance (ANOVA) using the JMP software
(SAS Institute Inc. Cary, NC, USA), and for comparisons of means
(Tukey's tests). The data show that each treatment studied did not
provide complete control against P. ramosa. However, the virulence
of the attacks was mitigated by some of the treatments tried: radicon
biostimulant, compost activated with Fusarium, mineral fertilizer
nitrogen, sulfur, enzone, and the resistant tomato genotype. It is
assumed that these effects can be improved by combining some of
these treatments with each other, especially for a gradual and
continuing reduction of the “seed bank” of the parasite in the soil.
Abstract: Soil quality monitoring is a science-based soil
management tool that assesses soil ecosystem health.
A soil monitoring program in Auckland, New Zealand’s largest
city extends from 1995 to the present. The objective of this study was
to firstly determine changes in soil parameters (basic soil properties
and heavy metals) that were assessed from rural land in 1995-2000
and repeated in 2008-2012. The second objective was to determine
differences in soil parameters across various land uses including
native bush, rural (horticulture, pasture and plantation forestry) and
urban land uses using soil data collected in more recent years (2009-
2013).
Across rural land, mean concentrations of Olsen P had
significantly increased in the second sampling period and was
identified as the indicator of most concern, followed by soil
macroporosity, particularly for horticultural and pastoral land. Mean
concentrations of Cd were also greatest for pastoral and horticultural
land and a positive correlation existed between these two parameters,
which highlights the importance of analysing basic soil parameters in
conjunction with heavy metals. In contrast, mean concentrations of
As, Cr, Pb, Ni and Zn were greatest for urban sites. Native bush sites
had the lowest concentrations of heavy metals and were used to
calculate a ‘pollution index’ (PI). The mean PI was classified as high
(PI > 3) for Cd and Ni and moderate for Pb, Zn, Cr, Cu, As and Hg,
indicating high levels of heavy metal pollution across both rural and
urban soils. From a land use perspective, the mean ‘integrated
pollution index’ was highest for urban sites at 2.9 followed by
pasture, horticulture and plantation forests at 2.7, 2.6 and 0.9,
respectively.
It is recommended that soil sampling continues over time because
a longer spanning record will allow further identification of where
soil problems exist and where resources need to be targeted in the
future. Findings from this study will also inform policy and science
direction in regional councils.
Abstract: Silica was extracted from agriculture waste rice husk
ash (RHA) and was used as the silica source for synthesis of
RMCM-48 and RSBA-16. An alkali fusion process was utilized to
separate silicate supernatant and the sediment effectively. The
CTAB/Si and F127/Si molar ratio was employed to control the
structure properties of the obtained RMCM-48 and RSBA-16
materials. The N2 adsorption-desorption results showed the
micro-mesoporous RSBA-16 possessed high specific surface areas
(662-1001 m2/g). All the obtained RSBA-16 materials were applied as
the adsorbents for acetone adsorption. And the breakthrough tests
clearly revealed that the RSBA-16(0.004) materials could achieve the
highest acetone adsorption capacity of 181 mg/g under 1000 ppmv
acetone vapor concentration at 25oC, which was also superior to
ZSM-5 (71mg/g) and MCM-41 (157mg/g) under same test conditions.
This can help to reduce the solid waste and the high adsorption
performance of the obtained materials could consider as potential
adsorbents for acetone adsorption.
Abstract: Morphological interaction of porcine cumulus-oocyte
complexes (pCOCs) was investigated on in vitro condition using
electron microscope (SEM and TEM). The totals of 1,923 oocytes
were round in shape, surrounded by Zona pellucida with layer of
cumulus cells ranging between 59.29-202.14 μm in size. They were
classified into intact-, multi-, partial cumulus cell layer oocyte, and
completely denuded oocyte, at the percentage composition of 22.80%
32.70%, 18.60%, and 25.90 % respectively. The pCOCs classified as
intact- and multi cumulus cell layer oocytes were further culturing at
37°C with 5% CO2, 95% air atmosphere and high humidity for 44 h
in M199 with Earle’s salts supplemented with 10% HTFCS, 2.2
mg/mL NaHCO3, 1 M Hepes, 0.25 mM pyruvate, 15 μg/mL porcine
follicle-stimulating hormone, 1 μg/mL LH, 1μg/mL estradiol with
ethanol, and 50 μg/mL gentamycin sulfate. On electron microscope
study, cumulus cells were found to stick their processes to secrete
substance from the sac-shape end into Zona pellucida of the oocyte
and also communicated with the neighboring cells through their
microvilli on the beginning of incubation period. It is believed that
the cumulus cells communicate with the oocyte by inserting the
microvilli through this gap and embedded in the oocyte cytoplasm
before secreting substance, through the sac-shape end of the
microvilli, to inhibit primary oocyte development at the prophase I.
Morphological changes of the complexes were observed after
culturing for 24-44 h. One hundred percentages of the cumulus layers
were expanded and cumulus cells were peeling off from the oocyte
surface. In addition, the round-shape cumulus cells transformed
themselves into either an elongate shape or a columnar shape, and no
communication between cumulus neighboring cells. After 44 h of
incubation time, diameter of oocytes surrounded by cumulus cells
was larger than 0 h incubation. The effect of hormones in culture
medium is exerted by their receptors present in porcine oocyte. It is
likely that all morphological changes of the complexes after hormone
treatment were to allow maturation of the oocyte. This study
demonstrated that the association of hormones in M199 could
promote porcine follicle activation in 44 h in vitro condition. This
culture system should be useful for studying the regulation of early
follicular growth and development, especially because these follicles represent a large source of oocytes that could be used in vitro for cell
technology.