Selection of Pichia kudriavzevii Strain for the Production of Single-Cell Protein from Cassava Processing Waste
A total of 115 yeast strains isolated from local cassava
processing wastes were measured for crude protein content. Among
these strains, the strain MSY-2 possessed the highest protein
concentration (>3.5 mg protein/mL). By using molecular
identification tools, it was identified to be a strain of Pichia
kudriavzevii based on similarity of D1/D2 domain of 26S rDNA
region. In this study, to optimize the protein production by MSY-2
strain, Response Surface Methodology (RSM) was applied. The
tested parameters were the carbon content, nitrogen content, and
incubation time. Here, the value of regression coefficient (R2) =
0.7194 could be explained by the model which is high to support the
significance of the model. Under the optimal condition, the protein
content was produced up to 3.77 g per L of the culture and MSY-2
strain contains 66.8 g protein per 100 g of cell dry weight. These
results revealed the plausibility of applying the novel strain of yeast
in single-cell protein production.
[1] O. Chaisinboon, and J. Chontanawat, "Factors determining the
competing use of Thailand's cassava for food and fuel," Energy
Procedia, vol. 9, 2011, pp. 216-229.
[2] T. Silalertruksa, S.H. Gheewala, K. Hunecke, and U.R. Fritsche,
"Biofuels and employment effects: Implications for socio-economic
development in Thailand," Biomass and Bioenergy, vol. 46, 2012, pp.
409-418.
[3] M. Davis, C.V. Maxwell, and T.J. Wistuba, "Dietary supplementation
with phosphorylate mannans improve growth responses and modulates
immune function of weanling pig," Journal of Animal Science, vol. 84,
2004, pp. 1882-1891.
[4] Anupama, and P. Ravindra, "Value-added food single cell protein,"
Biotechnol Adv, vol. 18, 2000, pp. 459-479.
[5] A. Chatzifragkou, A. Makri, A. Belka, and S. Bellou, "Biotechnological
conversions of biodiesel derived waste glycerol by yeast and fungal
species," Energy, vol. 36, 2011, pp. 1097-1108.
[6] C. Evans, and C. Ratledge, "A comparison of the oleaginous yeast
Candida curvata, grown on different carbon sources in continuous and
batch culture," Lipids, vol.18, 1983, pp. 623-629.
[7] J. Yadav, J. Bezawada, S. Elharche, S. Yan, R. Tyagi, and R.
Surampalli, "Simultaneous single-cell protein production and COD
removal with characterization of residual protein," Bioprocess Biosyst
Eng, vol. 37(6), 2013, pp. 1017-1029.
[8] J. Yadav, J. Bezawada, C. Ajila, S. Yan, R. Tyagi, and R. Surampalli,
"Mixed culture of Kluyveromyces marxianus and Candida krusei for
single-cell protein production and organic load removal from whey,"
Biores Technol, vol.164, 2014, pp. 119-127.
[9] M. Bradford, "Rapid and sensitive method for the quantitation of
microgram quantities of protein utilizing the principle of protein-dye
binding," Anal Biochem, vol.72, 1976, pp. 248–254.
[10] C. Kurtzman, and C.J. Robnett, "Identification and phylogeny of
ascomycetous yeasts from analysis of nuclear large subunit (26S)
ribosomal DNA partial sequences," Antonie van Leeuwenhoek, vol.
73(4), 1998, pp. 331-371. [11] S. Bringans, S. Eriksen, T. Kendrick, P. Gopalakrishnakone, A. Livk,
and R. Lock, "Proteomic analyses of the venom of Heterometrus
longimanus (Asian black scorpion)," Proteomics, vol. 8, 2008, pp. 1081-
1096.
[12] G. Chan, H.M. Gan, H.L. Ling, and N.A. Rashid, "Genome sequence of
Pichia kudriavzevii M12, a potential producer of bioethanol and
phytase," Eukaryot Cell, vol. 11(10), 2012, pp. 1300-1301.
[13] S. Moon, M. Chang, H.Y. Kim, and H.C. Chang, "Pichia kudriavzevii is
the major yeast involved in film-formation, off-odor production, and
texture-softening in over-ripened Kimchi," Food Science and
Biotechnology, vol. 23(2), 2014, pp. 489-497.
[14] N. Yuangsaard, W. Yongmanitchai, M. Yamada, and S. Limtong,
"Selection and characterization of a newly isolated thermotolerant Pichia
kudriavzevii strain for ethanol production at high temperature from
cassava starch hydrolysate," Antonie Van Leeuwenhoek, vol. 103(3),
2013, pp. 577-588.
[1] O. Chaisinboon, and J. Chontanawat, "Factors determining the
competing use of Thailand's cassava for food and fuel," Energy
Procedia, vol. 9, 2011, pp. 216-229.
[2] T. Silalertruksa, S.H. Gheewala, K. Hunecke, and U.R. Fritsche,
"Biofuels and employment effects: Implications for socio-economic
development in Thailand," Biomass and Bioenergy, vol. 46, 2012, pp.
409-418.
[3] M. Davis, C.V. Maxwell, and T.J. Wistuba, "Dietary supplementation
with phosphorylate mannans improve growth responses and modulates
immune function of weanling pig," Journal of Animal Science, vol. 84,
2004, pp. 1882-1891.
[4] Anupama, and P. Ravindra, "Value-added food single cell protein,"
Biotechnol Adv, vol. 18, 2000, pp. 459-479.
[5] A. Chatzifragkou, A. Makri, A. Belka, and S. Bellou, "Biotechnological
conversions of biodiesel derived waste glycerol by yeast and fungal
species," Energy, vol. 36, 2011, pp. 1097-1108.
[6] C. Evans, and C. Ratledge, "A comparison of the oleaginous yeast
Candida curvata, grown on different carbon sources in continuous and
batch culture," Lipids, vol.18, 1983, pp. 623-629.
[7] J. Yadav, J. Bezawada, S. Elharche, S. Yan, R. Tyagi, and R.
Surampalli, "Simultaneous single-cell protein production and COD
removal with characterization of residual protein," Bioprocess Biosyst
Eng, vol. 37(6), 2013, pp. 1017-1029.
[8] J. Yadav, J. Bezawada, C. Ajila, S. Yan, R. Tyagi, and R. Surampalli,
"Mixed culture of Kluyveromyces marxianus and Candida krusei for
single-cell protein production and organic load removal from whey,"
Biores Technol, vol.164, 2014, pp. 119-127.
[9] M. Bradford, "Rapid and sensitive method for the quantitation of
microgram quantities of protein utilizing the principle of protein-dye
binding," Anal Biochem, vol.72, 1976, pp. 248–254.
[10] C. Kurtzman, and C.J. Robnett, "Identification and phylogeny of
ascomycetous yeasts from analysis of nuclear large subunit (26S)
ribosomal DNA partial sequences," Antonie van Leeuwenhoek, vol.
73(4), 1998, pp. 331-371. [11] S. Bringans, S. Eriksen, T. Kendrick, P. Gopalakrishnakone, A. Livk,
and R. Lock, "Proteomic analyses of the venom of Heterometrus
longimanus (Asian black scorpion)," Proteomics, vol. 8, 2008, pp. 1081-
1096.
[12] G. Chan, H.M. Gan, H.L. Ling, and N.A. Rashid, "Genome sequence of
Pichia kudriavzevii M12, a potential producer of bioethanol and
phytase," Eukaryot Cell, vol. 11(10), 2012, pp. 1300-1301.
[13] S. Moon, M. Chang, H.Y. Kim, and H.C. Chang, "Pichia kudriavzevii is
the major yeast involved in film-formation, off-odor production, and
texture-softening in over-ripened Kimchi," Food Science and
Biotechnology, vol. 23(2), 2014, pp. 489-497.
[14] N. Yuangsaard, W. Yongmanitchai, M. Yamada, and S. Limtong,
"Selection and characterization of a newly isolated thermotolerant Pichia
kudriavzevii strain for ethanol production at high temperature from
cassava starch hydrolysate," Antonie Van Leeuwenhoek, vol. 103(3),
2013, pp. 577-588.
@article{"International Journal of Biological, Life and Agricultural Sciences:69868", author = "Phakamas Rachamontree and Theerawut Phusantisampan and Natthakorn Woravutthikul and Peerapong Pornwongthong and Malinee Sriariyanun", title = "Selection of Pichia kudriavzevii Strain for the Production of Single-Cell Protein from Cassava Processing Waste", abstract = "A total of 115 yeast strains isolated from local cassava
processing wastes were measured for crude protein content. Among
these strains, the strain MSY-2 possessed the highest protein
concentration (>3.5 mg protein/mL). By using molecular
identification tools, it was identified to be a strain of Pichia
kudriavzevii based on similarity of D1/D2 domain of 26S rDNA
region. In this study, to optimize the protein production by MSY-2
strain, Response Surface Methodology (RSM) was applied. The
tested parameters were the carbon content, nitrogen content, and
incubation time. Here, the value of regression coefficient (R2) =
0.7194 could be explained by the model which is high to support the
significance of the model. Under the optimal condition, the protein
content was produced up to 3.77 g per L of the culture and MSY-2
strain contains 66.8 g protein per 100 g of cell dry weight. These
results revealed the plausibility of applying the novel strain of yeast
in single-cell protein production.", keywords = "Single cell protein, response surface methodology,
yeast, cassava processing waste.", volume = "9", number = "5", pages = "517-5", }
