Abstract: This work describes refrigeration effects during storage on total protein and amino acids composition of raw and processed flour of two pearl millet cultivars (Ashana and Dembi). The protein content of the whole raw flour was found to be 14.46 and 13.38% for Ashana and Dembi cultivars, respectively. Dehulling of the grains reduced the protein content to 13.38 and 12.67% for the cultivars, respectively. For both cultivars, the protein content of the whole and dehulled raw flour before and after cooking was slightly decreased when the flour was stored for 60 days even after refrigeration. The effect of refrigeration process in combination with the storage period, cooking or dehulling was found to be vary between amino acids and even between cultivars. Regardless of the storage period and processing method, the amino acids content was remained unchanged after refrigeration for both cultivars.
Abstract: Twenty - nine Holstein cows were used to evaluate the effects of different dry period (DP) lengths on milk yield and composition, some blood metabolites, and complete blood count (CBC). Cows were assigned to one of 2 treatments: 1) 60-d dry period, 2) 35-d DP. Milk yield, from calving to 60 days, was not different for cows on the treatments (p =0.130). Cows in the 35-d DP produced more milk protein and SNF compare with cows in treatment 1 (p ≤ 0.05). Serum glucose, non-esterified fatty acids (NEFA), beta hydroxyl butyrate acid (BHBA), blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body condition score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. The results of this study demonstrated we can reduce the dry period length to 35 days with no problems.
Abstract: Proteins or genes that have similar sequences are likely to perform the same function. One of the most widely used techniques for sequence comparison is sequence alignment. Sequence alignment allows mismatches and insertion/deletion, which represents biological mutations. Sequence alignment is usually performed only on two sequences. Multiple sequence alignment, is a natural extension of two-sequence alignment. In multiple sequence alignment, the emphasis is to find optimal alignment for a group of sequences. Several applicable techniques were observed in this research, from traditional method such as dynamic programming to the extend of widely used stochastic optimization method such as Genetic Algorithms (GAs) and Simulated Annealing. A framework with combination of Genetic Algorithm and Simulated Annealing is presented to solve Multiple Sequence Alignment problem. The Genetic Algorithm phase will try to find new region of solution while Simulated Annealing can be considered as an alignment improver for any near optimal solution produced by GAs.
Abstract: To understand life as biological system, evolutionary
understanding is indispensable. Protein interactions data are rapidly
accumulating and are suitable for system-level evolutionary analysis.
We have analyzed yeast protein interaction network by both
mathematical and biological approaches. In this poster presentation,
we inferred the evolutionary birth periods of yeast proteins by
reconstructing phylogenetic profile. It has been thought that hub
proteins that have high connection degree are evolutionary old. But
our analysis showed that hub proteins are entirely evolutionary new.
We also examined evolutionary processes of protein complexes. It
showed that member proteins of complexes were tend to have
appeared in the same evolutionary period. Our results suggested that
protein interaction network evolved by modules that form the
functional unit. We also reconstructed standardized phylogenetic trees
and calculated evolutionary rates of yeast proteins. It showed that
there is no obvious correlation between evolutionary rates and
connection degrees of yeast proteins.
Abstract: Recent research has shown that milk proteins can
yield bioactive peptides with opioid, mineral binding,
cytomodulatory, antihypertensive, immunostimulating, antimicrobial
and antioxidative activity in the human body. Bioactive peptides are
encrypted in milk proteins and are only released by enzymatic
hydrolysis in vivo during gastrointestinal digestion, food processing
or by microbial enzymes in fermented products. At present
significant research is being undertaken on the health effects of
bioactive peptides. A variety of naturally formed bioactive peptides
have been found in fermented dairy products, such as yoghurt, sour
milk and cheese. In particular, antihypertensive peptides have been
identified in fermented milks, whey and ripened cheese. Some of
these peptides have been commercialized in the form of fermented
milks. Bioactive peptides have the potential to be used in the
formulation of health-enhancing nutraceuticals, and as potent drugs
with well defined pharmacological effects.
Abstract: Our objectives were to evaluate the effects of sire
breed, type of protein supplement, level of supplementation and sex
on wool spinning fineness (SF), its correlations with other wool
characteristics and prediction accuracy in F1 Merino crossbred lambs.
Texel, Coopworth, White Suffolk, East Friesian and Dorset rams
were mated with 500 purebred Merino dams at a ratio of 1:100 in
separate paddocks within a single management system. The F1
progeny were raised on ryegrass pasture until weaning, before forty
lambs were randomly allocated to treatments in a 5 x 2 x 2 x 2
factorial experimental design representing 5 sire breeds, 2
supplementary feeds (canola or lupins), 2 levels of supplementation
(1% or 2% of liveweight) and sex (wethers or ewes). Lambs were
supplemented for six weeks after an initial three weeks of adjustment,
wool sampled at the commencement and conclusion of the feeding
trial and analyzed for SF, mean fibre diameter (FD), coefficient of
variation (CV), standard deviation, comfort factor (CF), fibre
curvature (CURV), and clean fleece yield. Data were analyzed using
mixed linear model procedures with sire fitted as a random effect,
and sire breed, sex, supplementary feed type, level of
supplementation and their second-order interactions as fixed effects.
Sire breed (P
Abstract: Predicting protein-protein interactions represent a key step in understanding proteins functions. This is due to the fact that proteins usually work in context of other proteins and rarely function alone. Machine learning techniques have been applied to predict protein-protein interactions. However, most of these techniques address this problem as a binary classification problem. Although it is easy to get a dataset of interacting proteins as positive examples, there are no experimentally confirmed non-interacting proteins to be considered as negative examples. Therefore, in this paper we solve this problem as a one-class classification problem using one-class support vector machines (SVM). Using only positive examples (interacting protein pairs) in training phase, the one-class SVM achieves accuracy of about 80%. These results imply that protein-protein interaction can be predicted using one-class classifier with comparable accuracy to the binary classifiers that use artificially constructed negative examples.
Abstract: The worldwide prevalence of H3N2 influenza virus
and its increasing resistance to the existing drugs necessitates for the
development of an improved/better targeting anti-influenza drug.
H3N2 influenza neuraminidase is one of the two membrane-bound
proteins belonging to group-2 neuraminidases. It acts as key player
involved in viral pathogenicity and hence, is an important target of
anti-influenza drugs. Oseltamivir is one of the potent drugs targeting
this neuraminidase. In the present work, we have taken subtype N2
neuraminidase as the receptor and probable analogs of oseltamivir as
drug molecules to study the protein-drug interaction in anticipation of
finding efficient modified candidate compound. Oseltamivir analogs
were made by modifying the functional groups using Marvin Sketch
software and were docked using Schrodinger-s Glide. Oseltamivir
analog 10 was detected to have significant energy value (16% less
compared to Oseltamivir) and could be the probable lead molecule. It
infers that some of the modified compounds can interact in a novel
manner with increased hydrogen bonding at the active site of
neuraminidase and it might be better than the original drug. Further
work can be carried out such as enzymatic inhibition studies;
synthesis and crystallizing the drug-target complex to analyze the
interactions biologically.
Abstract: Four different colors of cowpeas (Vigna unguiculata)
(black, white, red and black/white speckled) and red kidney bean
(Phaseolus vulgaris) were used to evaluate proximate compositions,
starch content, and pasting properties. There were no significant
differences of moisture, protein, ash, fat, and carbohydrate contents
of all bean types. The kidney bean had significantly lower amounts of
total starch and solubilized starch compared to those of other
cowpeas (p ≤ 0.05), whereas the red cowpea and red kidney bean had
highest content of resistant starch (9-10%). Decortication indicated
no significant effect on the proximate compositions of all samples,
but it significantly decreased the resistant starch content in cowpeas
and increased the solubilized starch and total starch content in all
types of cowpeas. The highest values of pasting properties, generally
observed in flours obtained from black and black/white speckled
cowpea.
Abstract: The present study has been conducted to characterize
the prophenoloxidase (PPO) system of the desert locust, Schistocerca
gregaria following injection of Bacillus thuringiensis kurstaki (Bt).
The bulk of PPO system was associated with haemocytes and a little
amount was found in plasma. This system was activated by different
activators such as laminarin, lipopolysaccharide (LPS) and trypsin
suggesting that the stimulatory mechanism may involve an enzyme
cascade of one or more associated molecules. These activators did
not activate all the molecules of the cascade. Presence of
phenoloxidase activity (PO) coincides with the appearance of protein
band with molecular weight (MW) 70.154 KD (Kilo Dalton).
Abstract: Recent studies demonstrated that high-fat diet increases oxidative stress in plasma and in a variety of tissues. Many researchers have been looking for natural products, which can reverse the effect of high fat diet. Recently, buckwheat is becoming common ingredient in functional food because of it properties. In study on buckwheat, it is known that, this plant plays roles as anti-oxidative, anti-inflammatory and anti-hypertensive. Nevertheless still little is known about buckwheat groats. The aim of this study was to investigate the effects of addition of buckwheat groats to the fat diet (30% lard), on some antioxidant and oxidant stress parameters in plasma and selected tissues in Wistar rats. The experiment was carried out with three months old male Wistar rats ca. 250g of body weight fed for 5 weeks with either a high-fat (30% of lard) diet or control diet, with or without addition of buckwheat groats. In plasma biochemistry and the activities of the antioxidant enzymes were measured selected tissues: glutathione peroxidase (GPX), catalase (CAT) and the levels of total and reduced glutathione (GSH), free thiol groups (pSH), antioxidant potential of plasma (FRAP) and oxidant stress indices - proteins carbonyl groups (CO) and malonyldialdehyde concentration (MDA). Activity of catalase (CAT) in plasma of rats was significantly increased in buckwheat groats groups and activity of GPx3 in plasma of rats was decreased in buckwheat groups as compared to control group. The reduced glutathione (GSH) in plasma of rats was significantly increased and protein CO was significantly decreased in buckwheat groups as compared to controls. The lowered concentration of GSH was found in serum of rats fed buckwheat groats addition but it accompanied in 7-fold increase in reduced-to-oxidized glutatione ratio, significant increase in HDL and decrease in nonHDL concentration. Conclusions: Buckwheat groats indicate a beneficial effect in inhibiting protein and lipid peroxidation in rats and improved lipid profile. These results suggest that buckwheat groats exert a significant antioxidant potential and may be used as normal food constituent to ameliorate the oxidant-induced damage in organism.
Abstract: Neonatal lupus erythematous (NLE) is a rare disease marked by clinical characteristic and specific maternal autoantibody. Many cutaneous, cardiac, liver, and hematological manifestations could happen with affect of one organ or multiple. In this case, both babies were premature, low birth weight (LBW), small for gestational age (SGA) and born through caesarean section from a systemic lupus erythematous (SLE) mother. In the first case, we found a baby girl with dyspnea and grunting. Chest X ray showed respiratory distress syndrome (RDS) great I and echocardiography showed small atrial septal defect (ASD) and ventricular septal defect (VSD). She also developed anemia, thrombocytopenia, elevated C-reactive protein, hypoalbuminemia, increasing coagulation factors, hyperbilirubinemia, and positive blood culture of Klebsiella pneumonia. Anti-Ro/SSA and Anti-nRNP/sm were positive. Intravenous fluid, antibiotic, transfusion of blood, thrombocyte concentrate, and fresh frozen plasma were given. The second baby, male presented with necrotic tissue on the left ear and skin rashes, erythematous macula, athropic scarring, hyperpigmentation on all of his body with various size and facial haemorrhage. He also suffered from thrombocytopenia, mild elevated transaminase enzyme, hyperbilirubinemia, anti-Ro/SSA was positive. Intravenous fluid, methyprednisolone, intravenous immunoglobulin (IVIG), blood, and thrombocyte concentrate transfution were given. Two cases of neonatal lupus erythematous had been presented. Diagnosis based on clinical presentation and maternal auto antibody on neonate. Organ involvement in NLE can occur as single or multiple manifestations.
Abstract: Tumor cells have an invasive and metastatic phenotype
that is the main cause of death for cancer patients. Tumor
establishment and penetration consists of a series of complex
processes involving multiple changes in gene expression. In this study,
intraperitoneal administration of a high concentration of ascorbic acid
inhibited tumor establishment and decreased tumor mass in BALB/C
mice implanted with S-180 sarcoma cancer cells. To identify proteins
involved in the ascorbic acid-mediated inhibition of tumor
progression, changes in the tumor proteome associated with ascorbic
acid treatment of BALB/C mice implanted with S-180 were
investigated using two-dimensional gel electrophoresis and mass
spectrometry. Twenty protein spots were identified whose expression
was different between control and ascorbic acid treatment groups.
Abstract: Meat and meat products for human consumption are one of main sources of protein, amino acids, fatty acids, vitamins, and minerals. Popular variety of meat product is meatballs, which can be enriched with valuable product – Jerusalem artichoke powder, made from dried and grinded Jerusalem artichoke tubers, it is raw material with low-calorie, low fat, rich in dietary fibres, minerals, and vitamins. The results of this study indicate that that people could accept the new product - meatballs with Jerusalem artichoke powder and Jerusalem artichoke powder is suitable for meatballs preparation, in result them is possible to improve meatballs sensory and physical properties.
Abstract: An experiment was conducted in October 2008 due the ability replacement plant associate biofertilizers by chemical fertilizers and the qualifying rate of chemical N fertilizers at the moment of using this biofertilizers and the interaction of this biofertilizer on each other. This field experiment has been done in Persepolis (Throne of Jamshid) and arrange by using factorial with the basis of randomized complete block design, in three replication Azespirilium SP bacteria has been admixed with consistence 108 cfu/g and inoculated with seeds of wheat, The streptomyces SP has been used in amount of 550 gr/ha and concatenated on clay and for the qualifying range of chemical fertilizer 4 level of N chemical fertilizer from the source of urea (N0=0, N1=60, N2=120, N3=180) has been used in this experiment. The results indicated there were Significant differences between levels of Nitrogen fertilizer in the entire characteristic which has been measured in this experiment. The admixed Azespirilium SP showed significant differences between their levels in the characteristics such as No. of fertile ear, No. of grain per ear, grain yield, grain protein percentage, leaf area index and the agronomic fertilizer use efficiency. Due the interaction streptomyses with Azespirilium SP bacteria this actinomycet didn-t show any statistically significant differences between it levels.
Abstract: Bacterial molecular chaperone DnaK plays an essential role in protein folding, stress response and transmembrane targeting of proteins. DnaKs from many bacterial species, including Escherichia coli, Salmonella typhimurium and Haemophilus infleunzae are the molecular targets for the insect-derived antimicrobial peptide pyrrhocoricin. Pyrrhocoricin-like peptides bind in the substrate recognition tunnel. Despite the high degree of crossspecies sequence conservation in the substrate-binding tunnel, some bacteria are not sensitive to pyrrhocoricin. This work addresses the molecular mechanism of resistance of Helicobacter pylori DnaK to pyrrhocoricin. Homology modelling, structural and sequence analysis identify a single aminoacid substitution at the interface between the lid and the β-sandwich subdomains of the DnaK substrate-binding domain as the major determinant for its resistance.
Abstract: This research studied the simulation of increased
ambient ozone to estimate nutrient content and genetic changes in
two Thai soybean cultivars (Chiang Mai 60 and Srisumrong 1).
Ozone stress conditions affected proteins and lipids. It was found
that proteins decreased, but lipids increased. Srisumrong 1 cultivars
were more sensitive to ozone stress than Chiang Mai 60 cultivars.
The effect of ozone stress conditions on plant phenotype and
genotype was analyzed using the AFLP technique for the 2 Thai
soybean cultivars (Chiang Mai 60 and Srisumrong 1).
Abstract: There is strong evidence that water channel proteins
'aquaporins (AQPs)' are central components in plant-water relations
as well as a number of other physiological parameters. We had
previously reported the isolation of 24 plasma membrane intrinsic
protein (PIP) type AQPs. However, the gene numbers in rice and the
polyploid nature of bread wheat indicated a high probability of
further genes in the latter. The present work focused on identification
of further AQP isoforms in bread wheat. With the use of altered
primer design, we identified five genes homologous, designated
PIP1;5b, PIP2;9b, TaPIP2;2, TaPIP2;2a, TaPIP2;2b. Sequence
alignments indicate PIP1;5b, PIP2;9b are likely to be homeologues of
two previously reported genes while the other three are new genes
and could be homeologs of each other. The results indicate further
AQP diversity in wheat and the sequence data will enable physical
mapping of these genes to identify their genomes as well as genetic to
determine their association with any quantitative trait loci (QTLs)
associated with plant-water relation such as salinity or drought
tolerance.
Abstract: Protein subchloroplast locations are correlated with its
functions. In contrast to the large amount of available protein
sequences, the information of their locations and functions is less
known. The experiment works for identification of protein locations
and functions are costly and time consuming. The accurate prediction
of protein subchloroplast locations can accelerate the study of
functions of proteins in chloroplast. This study proposes a Random
Forest based method, ChloroRF, to predict protein subchloroplast
locations using interpretable physicochemical properties. In addition
to high prediction accuracy, the ChloroRF is able to select important
physicochemical properties. The important physicochemical
properties are also analyzed to provide insights into the underlying
mechanism.
Abstract: Nonspecific protein adsorption generally occurs on
any solid surfaces and usually has adverse consequences. Adsorption
of proteins onto a solid surface is believed to be the initial and
controlling step in biofouling. Surfaces modified with end-tethered
poly(ethylene glycol) (PEG) have been shown to be protein-resistant
to some degree. In this study, the adsorption of β-casein and
lysozyme was performed on 6 different types of surfaces where PEG
was tethered onto stainless steel by polyethylene imine (PEI) through
either OH or NHS end groups. Protein adsorption was also performed
on the bare stainless steel surface as a control. The adsorption was
conducted at 23 °C and pH 7.2. In situ QCM-D was used to
determine PEG adsorption kinetics, plateau PEG chain densities,
protein adsorption kinetics and plateau protein adsorbed quantities.
PEG grafting density was the highest for a NHS coupled chain,
around 0.5 chains / nm2. Interestingly, lysozyme which has smaller
size than β-casein, appeared to adsorb much less mass than that of β-
casein. Overall, the surface with high PEG grafting density exhibited
a good protein rejection.