Abstract: Pigeon pea (Cajanus cajan) blanched for 20min was dehulled and milled into flour. The flour was incorporated into dried whole fermented maize (Ogi) at five levels. The resultant products were analyzed for chemical and pasting properties. The fortified Ogi samples were also assessed for sensory attributes: appearance, color, flavor, mouth feel and overall acceptability. The protein content in the whole Ogi fortified samples was in the range of 11.2-16.6% and crude fibre 3.22-3.46%. Fortified whole Ogi with pigeon pea at 30%, 40% and 50% of inclusion with pigeon pea flour has higher protein, crude fibre and ash content. Varying range of pasting quality was recorded for the blends, pasting temperature for fortified Obi was in the range of 45.3-49.50C and peak time 5.05-5.210C. The sensory acceptability of the whole Ogi fortified blends prepared into gruel has higher acceptability for various qualities in comparison with the traditional Ogi gruel.
Abstract: The Egyptian Bacillus thuringiensis isolate (M5) produce crystal proteins that is toxic against insects was irradiated with UV light to induce mutants. Upon testing 10 of the resulting mutants for their toxicity against cotton leafworm larvae, the three mutants 62, 64 and 85 proved to be the most toxic ones. Upon testing these mutants along with their parental isolate by SDS-PAGE analysis of spores-crystals proteins as well as vegetative cells proteins, new induced bands appeared in the three mutants by UV radiation and also they showed disappearance of some other bands as compared with the wild type isolate. Multiplex PCR technique, with five sets of specific primers, was used to detect the three types of cryI genes cryIAa, cryIAb and cryIAc. Results showed that these three genes exist, as distinctive bands, in the wild type isolate (M5) as well as in mutants 62 and 85, while the mutant 64 had two distinctive bands of cryIAb and cryIAc genes, and a faint band of cryI Aa gene. Finally, these results revealed that mutant 62 is considered as the promising mutant since it is UV resistant, highly toxic against Spodoptera littoralis and active against a wide range of Lepidopteran insects.
Abstract: In order to characterize the soy protein hydrolysate obtained in this study, gel chromatography on Sephadex G-25 was used to perform the separation of the peptide mixture and electrophoresis in SDS-polyacrylamide gel has been employed. Protein hydrolysate gave high antioxidant activities, but didn't give any antimicrobial activities. The antioxidant activities of protein hydrolysate was in the same trend of peptide content which gave high antioxidant activities and high peptide content between fractions 15 to 50. With increasing peptide concentrations, the scavenging effect on DPPH radical increased until about 70%, thereafter reaching a plateau. In compare to different concentrations of BHA, which exhibited higher activity (90%), soybean protein hydrolysate exhibited high antioxidant activities (70%) at a concentration of 1.45 mg/ml at fraction 25. Electrophoresis analysis indicated that, low- MW hydrolysate fractions (F1) appeared, on average, to have higher DPPH scavenging activities than high-MW fractions. These results revealed that soybean peptides probably contain substances that were proton donors and could react with free radicals to convert them to stable diamagnetic molecules.
Abstract: The seeds of cotton (Gossypium hirsutum) fall among the lesser known oil seeds. Cottonseeds are not normally consumed in their natural state due to their gossypol content, an antinutrient. The effect of processing on the sensory characteristics and chemical composition of cottonseed and its extract was studied by subjecting the cottonseed extract to heat treatment (boiling) and the cottonseed to fermentation. The cottonseed extract was boiled using the open pot and the pressure pot for 30 minutes respectively. The fermentation of the cottonseed was carried out for 6 days with samples withdrawn at intervals of 2 days. The extract and fermented samples were subjected to chemical analysis and sensory evaluated for colour, aroma, taste, mouth feel, appearance and overallacceptability. The open pot sample was more preferred. Fermentation for 6 days resulted into a significant reduction in gossypol level of the cottonseed; however, sample fermented for 2 days was most preferred.
Abstract: Gilaburu (Viburnum opulus L.) grown naturally in
Anatolia. In this study, some physico-chemical (sugar, acid, protein,
crude fat, crude fiber, ash etc.) characteristics and mineral
composition of Gilaburu fruit have been investigated. The length,
width, thickness, weight, total soluble solid, protein, crude ash, crude
fiber and crude oil of fruit were found to be 1.12 cm, 1.58 cm, 1.87
cm, 0.87 g, 14.73 %, 0.2 %, 0.11 %, 6.56 % and 0.4 %, respectively.
The seed of fruit mean weight, length, width and thickness were
determinated as 0.08 g, 7.76 cm, 7.67 cm and 1.66, respectively. In
addition 27 mineral elements (Al, Mg, Na, Ba, Ca, Ni, Cd, P, Cr, Pb,
S, Cu, Se, Fe, K, Sr, Li, Z, V, Ag, Bi, Co, Mn, B, Ga, In, Ti) were
analyzed. Gilaburu (Viburnum opulus L.) fruit was richest in
potassium (10764.764 ppm), Mg (1289.088 ppm) and P (1304.169
ppm).
Abstract: Wheat germ has a balanced amino acid composition of the protein, which is well digested by enzymes in the gastrointestinal tract of humans, a high content of vitamins, minerals and unsaturated acids. Introduction components grain food products will enrich their biologically important substances, giving these products a number of valuable properties and reducing their caloric.
A complex natural system of substances in foods will help replenish the body's need of essential nutrients, increasing its resistance to the harmful effects of the environment, prolong life. In this regard, there was a need for the development of production technology of protein complexes from wheat germ and then applying them in food, particularly in the dairy industry. Experimental studies were conducted to determine the number of herbal supplements on the sensory characteristics of the product. Studies have been conducted to determine the optimal process parameters of water activity and moisture content of the investigational product.
Abstract: The prediction of transmembrane helical segments
(TMHs) in membrane proteins is an important field in the
bioinformatics research. In this paper, a method based on discrete
wavelet transform (DWT) has been developed to predict the number
and location of TMHs in membrane proteins. PDB coded as 1F88 was
chosen as an example to describe the prediction of the number and
location of TMHs in membrane proteins by using this method. One
group of test data sets that contain total 19 protein sequences was
utilized to access the effect of this method. Compared with the
prediction results of DAS, PRED-TMR2, SOSUI, HMMTOP2.0 and
TMHMM2.0, the obtained results indicate that the presented method
has higher prediction accuracy.
Abstract: The shelf life of fish was extended using disinfection
properties of ozone. For this purpose, Trout specimens were exposed
to ozone in the aqueous media for two hours and their microbial
growth and biochemical properties were measured over time.
Microbial growth of ozone treated fish was significantly slower than
control sample, resulting in lower counts of bacteria. According to
the biochemical tests; ozone treatment had no negative effects on fat,
protein and humidity of fish. Peroxide and TVN (Total Volatile
Nitrogen) measurements showed that treatment by ozone increased
the trout shelf life from 4 days to 6 days. According to the sensory
analysis, no changes were observed in color or flavor of the ozone
treated trout.
Abstract: Identifying protein coding regions in DNA sequences is a basic step in the location of genes. Several approaches based on signal processing tools have been applied to solve this problem, trying to achieve more accurate predictions. This paper presents a new predictor that improves the efficacy of three techniques that use the Fourier Transform to predict coding regions, and that could be computed using an algorithm that reduces the computation load. Some ideas about the combination of the predictor with other methods are discussed. ROC curves are used to demonstrate the efficacy of the proposed predictor, based on the computation of 25 DNA sequences from three different organisms.
Abstract: The usefulness of weaning foods to meet the nutrient
needs of children is well recognized, and most of them are precooked
roller dried mixtures of cereal and/or legume flours which posses a
high viscosity and bulk when reconstituted. The objective of this study
was to formulate composite weaning foods using cereals, malted
legumes and vegetable powders and analyze them for nutrients,
functional properties and sensory attributes. Selected legumes (green
gram and lentil) were germinated, dried and dehulled. Roasted wheat,
rice, carrot powder and skim milk powder also were used. All the
ingredients were mixed in different proportions to get four
formulations, made into 30% slurry and dried in roller drier. The
products were analyzed for proximate principles, mineral content,
functional and sensory qualities. The results of analysis showed
following range of constituents per 100g of formulations on dry
weight basis, protein, 18.1-18.9 g ; fat, 0.78-1.36 g ; iron, 5.09-6.53
mg; calcium, 265-310 mg. The lowest water absorption capacity was
in case of wheat green gram based and the highest was in rice lentil
based sample. Overall sensory qualities of all foods were graded as
“good" and “very good" with no significant differences. The results
confirm that formulated weaning foods were nutritionally superior,
functionally appropriate and organoleptically acceptable.
Abstract: The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.
Abstract: P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin-s lymphoma (HL) and nonHodgkin-s lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (30%). In subtypes of HL, P16 loss was found exclusively in all cases of lymphocyte depletion, lymphocyte predominance and unclassified cases, whereas P16 loss was seen in 39% of mixed cellularity and 29% of nodular sclerosis cases. In low grade NHL patients, P16 loss was seen in approximately one-third of cases, whereas no or very rare of P16 loss was found in intermediate and high grade cases. P16 loss did not show any correlation with age or gender of NHL patients. In conclusion, the high rate of P16 loss seen in our study suggests that loss of P16 expression plays a critical role in the pathogenesis of lymphoma, particularly with HL.
Abstract: Atherosclerosis was identified as a chronic inflammatory process resulting from interactions between plasma lipoproteins, cellular components (monocyte, macrophages, T lymphocytes, endothelial cells and smooth muscle cells) and the extracellular matrix of the arterial wall. Several types of genes were known to express during formation of atherosclerosis. This study is carried out to identify unknown differentially expressed gene (DEG) in atherogenesis. Rabbit’s aorta tissues were stained by H&E for histomorphology. GeneFishing™ PCR analysis was performed from total RNA extracted from the aorta tissues. The DNA fragment from DEG was cloned, sequenced and validated by Real-time PCR. Histomorphology showed intimal thickening in the aorta. DEG detected from ACP-41 was identified as cathepsin B gene and showed upregulation at week-8 and week-12 of atherogenesis. Therefore, ACP-based GeneFishing™ PCR facilitated identification of cathepsin B gene which was differentially expressed during development of atherosclerosis.
Abstract: The influence of flakes from biologically activated
hull-less barley grain and malt extract on chemical composition of
yoghurt was studied.
Pasteurized milk, freeze-dried yoghurt culture YF-L811 (Chr.
Hansen, Denmark), flakes from biologically activated hull-less
barley grain (Latvia) and malt extract (Ilgezeem, Latvia) were used
for experiments. Yoghurt samples with and without flakes from
biologically activated hull-less barley grain and malt extract were
analyzed for content of total solids, total proteins, fats, amino acids
and riboflavin.
The addition of flakes from biologically activated hull-less barley
grain and malt extract allowed increase of nutritional value of
yoghurt samples. There was obtained the increase of total proteins
(p>0.05) and the decrease of fat (p>0.05). The presence of flakes
from biologically activated hull-less barley grain and malt extract in
yoghurt samples provided significant increase of amino acids amount
(p
Abstract: This experiment was conducted to investigate the
effect of different levels of dietary chromium yeast (Cr-yeast) on
thigh meat quality of broiler chicks reared under heat stress
condition. Two hundred and forty Ross male chickens in heat stress
condition (33±3°C) were allocated to five treatments in a completely
randomized design. Treatments were supplemented with 0 (control),
200, 400, 800 and 1200 μg kg-1 Cr in the form of Cr yeast. Twelve
chicks from each treatment were slaughtered at 42 d, to evaluate
moisture, protein, lipid, pH and lipid oxidation of thigh meat.
Protein, moisture, lipid and pH of thigh meat were not affected by
supplemental Cr. Thigh meat lipid tended to decrease in broilers
received 1200 μg kg-1. Storage time increased lipid oxidation of
meat (P
Abstract: This research studied the hypoglycemic effect of
water soluble polysaccharide (WSP) extracted from yam (Dioscorea
hispida) tuber by three different methods: aqueous extraction, papain
assisted extraction, and tempeh inoculums assisted extraction. The
two later extraction methods were aimed to remove WSP binding
protein to have more pure WSP. The hypoglycemic activities were
evaluated by means in vivo test on alloxan induced hyperglycemic
rats, glucose response test (GRT), in situ glucose absorption test
using everted sac, and short chain fatty acids (SCFAs) analysis. All
yam WSP extracts exhibited ability to decrease blood glucose level in
hyperglycemia condition as well as inhibited glucose absorption and
SCFA formation. The order of hypoglycemic activity was tempeh
inoculums assisted- >papain assisted- >aqueous WSP extracts. GRT
and in situ glucose absorption test showed that order of inhibition
was papain assisted- >tempeh inoculums assisted- >aqueous WSP
extracts. Digesta of caecum of yam WSP extracts oral fed rats had
more SCFA than control. Tempeh inoculums assisted WSP extract
exhibited the most significant hypoglycemic activity.
Abstract: The aim of the present study was to investigate the
chemical and biological properties of local cowpea seed protein
cultivated in Gizan region. The results showed that the cowpea and
its products contain high level of protein (22.9-77.6%), high
carbohydrates (9.4-64.3%) and low fats (0.1-0.3%). The trypsin and
chymotrypsin activities were found to be 32.2 and 15.2 units,
respectively. These activities were not affected in both defatted and
protein concentrate whereas they were significantly reduced in
isolated protein and cooked samples. The phytate content of cooked
and concentrated cowpea samples varied from 0.25% -0.32%,
respectively. Tannin content was found to be 0.4% and 0.23% for
cooked and raw samples, respectively. The in vitro protein
digestibility was very high in cowpea seeds (75.04-78.76%). The
biological evaluation using rats showed that the group fed with
animal feed containing casein gain more weight than those fed with
that containing cowpea. However, the group fed with cooked cowpea
gain more weight than those fed with uncooked cowpea. On the
other hand, in vivo digestion showed high value (98.33%) among the
group consumed casein compared to other groups those consumed
cowpea contains feed. This could be attributed to low antinutritional
factors in casein contains feed compared to those of cowpea contains
feed because cooking significantly increased the digestion rate
(80.8% to 83.5%) of cowpea contains feed. Furthermore, the
biological evaluation was high (91.67%) of casein containing feed
compared to that of cowpea containing feed (80.83%-87.5%). The
net protein utilization (NPU) was higher (89.67%) in the group fed
with casein containing feed than that of cowpea containing feed
(56.33%-69.67%).
Abstract: This paper describes a novel approach for deriving
modules from protein-protein interaction networks, which combines
functional information with topological properties of the network.
This approach is based on weighted clustering coefficient, which
uses weights representing the functional similarities between the
proteins. These weights are calculated according to the semantic
similarity between the proteins, which is based on their Gene
Ontology terms. We recently proposed an algorithm for identification
of functional modules, called SWEMODE (Semantic WEights for
MODule Elucidation), that identifies dense sub-graphs containing
functionally similar proteins. The rational underlying this approach is
that each module can be reduced to a set of triangles (protein triplets
connected to each other). Here, we propose considering semantic
similarity weights of all triangle-forming edges between proteins. We
also apply varying semantic similarity thresholds between
neighbours of each node that are not neighbours to each other (and
hereby do not form a triangle), to derive new potential triangles to
include in module-defining procedure. The results show an
improvement of pure topological approach, in terms of number of
predicted modules that match known complexes.
Abstract: We report a novel fusion tag for expressing
recombinant proteins in E. coli. The fusion tag is the C-terminus part
of the human GMCSF gene comprising 45 amino acids, which aid in
over expression of otherwise non expressible genes. Expression of
hIFN a2b with this fusion tag also escapes the requirement of rare
codons for expression. This is also a first report of a small fusion tag
of human origin having affinity to heparin sepharose column
facilitating the purification of fusion protein.
Abstract: To assess the effects of functional protein on osteoblast, Large quantity of high-purity osteoblasts had been cultivated successfully by adopting sequential enzyme digestion. The growth curve of osteoblasts was protracted by cell counting. Proliferation of osteoblasts was assessed by MTT colorimetry. The experimental results show the functional protein can enhance proliferation, the properties of adhesion and discuss the effect of osteopontin on osteoblast.