The Effect of Buckwheat (Fagopyrum esculentum Moench) Groats Addition to the Lard Diet on Antioxidant Parameters of Plasma and Selected Tissues in Wistar Rats
Recent studies demonstrated that high-fat diet increases oxidative stress in plasma and in a variety of tissues. Many researchers have been looking for natural products, which can reverse the effect of high fat diet. Recently, buckwheat is becoming common ingredient in functional food because of it properties. In study on buckwheat, it is known that, this plant plays roles as anti-oxidative, anti-inflammatory and anti-hypertensive. Nevertheless still little is known about buckwheat groats. The aim of this study was to investigate the effects of addition of buckwheat groats to the fat diet (30% lard), on some antioxidant and oxidant stress parameters in plasma and selected tissues in Wistar rats. The experiment was carried out with three months old male Wistar rats ca. 250g of body weight fed for 5 weeks with either a high-fat (30% of lard) diet or control diet, with or without addition of buckwheat groats. In plasma biochemistry and the activities of the antioxidant enzymes were measured selected tissues: glutathione peroxidase (GPX), catalase (CAT) and the levels of total and reduced glutathione (GSH), free thiol groups (pSH), antioxidant potential of plasma (FRAP) and oxidant stress indices - proteins carbonyl groups (CO) and malonyldialdehyde concentration (MDA). Activity of catalase (CAT) in plasma of rats was significantly increased in buckwheat groats groups and activity of GPx3 in plasma of rats was decreased in buckwheat groups as compared to control group. The reduced glutathione (GSH) in plasma of rats was significantly increased and protein CO was significantly decreased in buckwheat groups as compared to controls. The lowered concentration of GSH was found in serum of rats fed buckwheat groats addition but it accompanied in 7-fold increase in reduced-to-oxidized glutatione ratio, significant increase in HDL and decrease in nonHDL concentration. Conclusions: Buckwheat groats indicate a beneficial effect in inhibiting protein and lipid peroxidation in rats and improved lipid profile. These results suggest that buckwheat groats exert a significant antioxidant potential and may be used as normal food constituent to ameliorate the oxidant-induced damage in organism.
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T. Sibley, D. R. Jacobs, and J. A. Nettleton, “Dietary intake of saturated
fat by food source and incident cardiovascular disease: the Multi-Ethnic
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2010.
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and I. A., Castro, “Effect of red wines with different in vitro antioxidant
activity on oxidative stress of high-fat diet rats, ”, Food Chem., vol. 15,
no. 137(1-4), pp.122-129, October 2013.
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technological process during buckwheat groats production on dietary
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279–283, July 2012.
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Technol. Aliment.,vol. 8, no. 2, pp.75-83, February 2009.
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Buckwheat Seeds and Groats,” J. Am. Oil. Chem. Soc., vol. 87, pp.
559–566, December 2010.
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Xue, and Y.M. Wu, “Bioactive compounds in functional buckwheat
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Fornal, “ Antioxidant properties and rutin content in high pressure treated
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pp. 92-100, August 2013.
[9] L. Sedej, M. Sakac, A. Mandic, A. Misan, V.Tumbas, and J.
Canadanovic-Brunet,“Buckwheat (Fagopyrum esculentum Moench)
Grain and Fractions: Antioxidant Compounds and Activities, ” Journal of
Food Science, vol. 77, pp. 954-959, September 2012.
[10] C. Wei, J. C. Wei, S. Zhi-Rong, and Y.Y Ping, “Protective effects of
ethanolic extracts of buckwheat groats on DNA damage caused by
hydroxyl radicals,” Food Res., Int., vol. 41 pp. 924–92, November 2008.
[11] I. F. Benzie, and J.J. Strain, “The ferric reducing ability of plasma (FRAP)
as a measure of "antioxidant power": the FRAP assay,” Analytical
Biochemistry, vol. 15, pp. 70-76, January 1996.
[12] R. Francik, M. Krośniak, M. Barlik, A. Kudła, R. Gryboś, and T.
Librowski, T., “Impact of Vanadium Complexes Treatment on the
Oxidative Stress Factors in Wistar Rats Plasma,” Bioinorganic Chemistry
and Applications, Article ID 206316, pp. 1-8., July 2011.
[13] W. C. Dornas, W. G. de Lima, R. C. Dos Santos, M. O. de Souza, M.
Silva, M. F. Diniz, and M. E. Silva M.E.,”Salt overload in fructose-fed
insulin-resistant rats decreases paraoxonase-1 activity,” Nutition &
Metabolism, vol. 9, no. 63, pp. 1-5, June 2012.
[14] M. Dobiasova, and J. Frohlich J.,”The plasma parameter log
(TG/HDL-C) as an atherogenic index: correlation with lipoprotein
particle size and estrification rate in apo B-lipoprotein-depleted plasma
(FERHDL),” Clinical Biochemistry, vol. 34, pp. 583-88, October 2001.
[15] R. D. Malheiros, V.M.B. Moraes, A. Collin, G.P.J. Janssens, E.
Decuypere and J. Buyse,”Dietary macronutrients, endocrine functioning
and intermediary metabolism in broiler chickens:Pair wise substitutions
between protein, fat and carbohydrate”. Nutrition Research, vol. 23, pp.
567-578, April 2003.
[16] M. Ming, L. Guanhua., Y. Zhanhai, C. Guang, and Z. Xuan,”Effect of the
Lycium barbarum polysaccharides administration on blood lipid
metabolism and oxidative stress of mice fed high-fat diet in vivo,” Food
Chemistry, vol. 113, no. 4, pp. 872-877, April 2009.
[17] H. Chen, L.J. Liu, J.J. Zhu J. B. Xu, and R. Li,”Effect of soybean
oligosaccharides on blood lipid, glucose levels and antioxidant enzymes
activity in high fat rats,” Food Chemistry, vol.119, no. 4, pp. 1633-1636,
April 2010.
[18] C. Xu, Z. Haiyan, Z. Hua, Z Jianhong, and D. Pin, ”Effect of Curcuma
kwangsiensis polysaccharides on blood lipid profiles and oxidative stress
in high-fat rats,” International Journal of Biological Macromolecules,
vol. 44, no. 2, pp. 138-142, March 2009.
[19] Y. K. Hong, H. T.Wu, T. Ma, W. J. Liu, and X. J. He, “Effects of
Glycyrrhiza glabra polysaccharides on immune and antioxidant activities
in high-fat mice,” International Journal of Biological Macromolecules,
vol. 45, no. 1, pp. 61-64, July 2009.
[20] M. Wang, J. R. Liu, J. M. Gao, J.W. Parry, and Y.M. Wie, ”Antioxidant
activity of tartary buckwheat bran extract and its effect on the lipid profile
of hyperlipidemic rats” Journal of Agricultural and Food Chemistry, vol.
57, no. 11, pp. 5106-5112, May 2009.
[21] L. Y. Lin, C.C. Peng, Y. L. Yang, and R. Y. Peng, “Optimization of
bioactive compounds in buckwheat sprouts and their effect on blood
cholesterol in hamsters,” Journal of Agricultural and Food Chemistry,
vol. 56, no. 4, pp. 1216-1223, January 2008.
[22] T. Katsube, M. Yamasaki, K. Shiwaku, T. Ishijima, I. Matsumoto, K.
Abe, and Y. Yamasaki, “ Effect of flavonol glycoside in mulberry (Morus
alba L.) leaf on glucose metabolism and oxidative stress in liver in
diet-induced obese mice,” Journal of the Science of Food and
Agriculture, vol. 90, no. 14, pp. 2386-2392, July 2010.
[23] S. M. B. Asdaq, and M.N. Inamdar, “Potential of Crocus sativus (saffron)
and its Constituent, Crocin, as Hypolipidemic and Antioxidant in Rats,”
Applied Biochemistry and Biotechnology, vol. 162, no. 2, pp. 358-372,
August 2010.
[24] R.L. Corwin, “ Effects of dietary fats on bone health in advanced age, “
Prostaglandins Leukot. Essent. Fatty Acids, vol. 68, no. 6, pp. 379–86,
June 2003.
[25] M. Oliveira de Souza, M. Silva, M. E. Silva, R. de Paula, R. Oliveira, and
M.L Pedrosa, “Diet supplementation with acai (Euterpe oleracea Mart.)
pulp improves biomarkers of oxidative stress and the serum lipid profile
in rats,” Nutrition, vol. 26, no. 7-8,pp. 804-810, July-August 2010.
[26] M. Watanabe, and J. Ayugase, “Effects of buckwheat sprouts on plasma
and hepatic parameters in type 2 diabetic db/db mice,” Journal of Food
Science, vol. 75, no. 9, pp. 294-299, November/December 2010.
[27] J.M. Kawa, C.G. Taylor, and R. Przybylski, ”Buckwheat concentrate
reduces serum glucose in streptozotocin-diabetic rats, “ Journal of
Agricultural and Food Chemistry, vol. 51, no.25, pp. 7287-7291,
November 2003.
[28] S.H. Heo, and S.H. Lee, “High levels of serum uric acid are associated
with silent brain infarction” Journal of the Neurological Sciences, vol.
297, no. 1-2, pp. 6-10, October 2010.
[29] K. A. Amin, H. H. Kamel, and M.A. Abd Eltawab, “The relation of high
fat diet, metabolic disturbances and brain oxidative dysfunction:
modulation by hydroxy citric acid,” Lipids in health and disease, vol. 10,
pp.74-85, May 2011.
[30] G. Préstamo, A. Pedrazuela, E. Peñas, M.A. Lasunción, and G. Arroyo,
“Role of buckwheat diet on rats as prebiotic and healthy food” Nutrition
Research, vol. 23, no. 6, pp. 803-814, June 2003.
[31] M.P. Shyamala, M. R. Venukumar, and M. S. Latha, “Antioxidant
potential of the Syzygium aromaticum (Gaertn.) Linn. (cloves) in rats fed
with high fat diet,” Indian Journal of Pharmacology, vol. 35, no. 2, pp.
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@article{"International Journal of Biological, Life and Agricultural Sciences:62482", author = "Chlopicka Joanna and Barton Henryk and Kryczyk Jadwiga and Francik Renata", title = "The Effect of Buckwheat (Fagopyrum esculentum Moench) Groats Addition to the Lard Diet on Antioxidant Parameters of Plasma and Selected Tissues in Wistar Rats", abstract = "Recent studies demonstrated that high-fat diet increases oxidative stress in plasma and in a variety of tissues. Many researchers have been looking for natural products, which can reverse the effect of high fat diet. Recently, buckwheat is becoming common ingredient in functional food because of it properties. In study on buckwheat, it is known that, this plant plays roles as anti-oxidative, anti-inflammatory and anti-hypertensive. Nevertheless still little is known about buckwheat groats. The aim of this study was to investigate the effects of addition of buckwheat groats to the fat diet (30% lard), on some antioxidant and oxidant stress parameters in plasma and selected tissues in Wistar rats. The experiment was carried out with three months old male Wistar rats ca. 250g of body weight fed for 5 weeks with either a high-fat (30% of lard) diet or control diet, with or without addition of buckwheat groats. In plasma biochemistry and the activities of the antioxidant enzymes were measured selected tissues: glutathione peroxidase (GPX), catalase (CAT) and the levels of total and reduced glutathione (GSH), free thiol groups (pSH), antioxidant potential of plasma (FRAP) and oxidant stress indices - proteins carbonyl groups (CO) and malonyldialdehyde concentration (MDA). Activity of catalase (CAT) in plasma of rats was significantly increased in buckwheat groats groups and activity of GPx3 in plasma of rats was decreased in buckwheat groups as compared to control group. The reduced glutathione (GSH) in plasma of rats was significantly increased and protein CO was significantly decreased in buckwheat groups as compared to controls. The lowered concentration of GSH was found in serum of rats fed buckwheat groats addition but it accompanied in 7-fold increase in reduced-to-oxidized glutatione ratio, significant increase in HDL and decrease in nonHDL concentration. Conclusions: Buckwheat groats indicate a beneficial effect in inhibiting protein and lipid peroxidation in rats and improved lipid profile. These results suggest that buckwheat groats exert a significant antioxidant potential and may be used as normal food constituent to ameliorate the oxidant-induced damage in organism.
", keywords = "Antioxidant, buckwheat, high-fat diet, rats.", volume = "7", number = "7", pages = "713-9", }
