Abstract: Liposomal magnetofection is a simple, highly efficient
technology for cell transfection, demonstrating better outcome than a
number of other common gene delivery methods. However,
aggregate complexes distribution over the cell surface is non-uniform
due to the gradient of the permanent magnetic field. The aim of this
study was to estimate the efficiency of liposomal magnetofection for
prostate carcinoma PC3 cell line using newly designed device,
“DynaFECTOR", ensuring magnetofection in a dynamic gradient
magnetic field. Liposomal magnetofection in a dynamic gradient
magnetic field demonstrated the highest transfection efficiency for
PC3 cells – it increased for 21% in comparison with liposomal
magnetofection and for 42% in comparison with lipofection alone.
The optimal incubation time under dynamic magnetic field for PC3
cell line was 5 minutes and the optimal rotation frequency of
magnets – 5 rpm. The new approach also revealed lower cytotoxic
effect to cells than liposomal magnetofection.
Abstract: Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.
Abstract: The present paper concerns with the influence of fiber
packing on the transverse plastic properties of metal matrix
composites. A micromechanical modeling procedure is used to
predict the effective mechanical properties of composite materials at
large tensile and compressive deformations. Microstructure is
represented by a repeating unit cell (RUC). Two fiber arrays are
considered including ideal square fiber packing and random fiber
packing defined by random sequential algorithm. The
micromechanical modeling procedure is implemented for
graphite/aluminum metal matrix composite in which the
reinforcement behaves as elastic, isotropic solids and the matrix is
modeled as an isotropic elastic-plastic solid following the von Mises
criterion with isotropic hardening and the Ramberg-Osgood
relationship between equivalent true stress and logarithmic strain.
The deformation is increased to a considerable value to evaluate both
elastic and plastic behaviors of metal matrix composites. The yields
strength and true elastic-plastic stress are determined for
graphite/aluminum composites.
Abstract: In this work, statistical experimental design was
applied for the optimization of medium constituents for Gentamicin
production by Micromsonospora echinospora subs pallida (MTCC
708) in a batch reactor and the results are compared with the ANN
predicted values. By central composite design, 50 experiments are
carried out for five test variables: Starch, Soya bean meal, K2HPO4,
CaCO3 and FeSO4. The optimum condition was found to be: Starch
(8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4
g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield
of gentamicin was found to be 1020 mg/L. The R2 values were found
to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for
RSM.
Abstract: In the present communication, stochastic comparison
of a series (parallel) system having heterogeneous components with
random lifetimes and series (parallel) system having homogeneous
exponential components with random lifetimes has been studied.
Further, conditions under which such a comparison is possible has
been established.
Abstract: This paper presents an information retrieval model on
XML documents based on tree matching. Queries and documents are
represented by extended trees. An extended tree is built starting from
the original tree, with additional weighted virtual links between each
node and its indirect descendants allowing to directly reach each
descendant. Therefore only one level separates between each node
and its indirect descendants. This allows to compare the user query
and the document with flexibility and with respect to the structural
constraints of the query. The content of each node is very important to
decide weither a document element is relevant or not, thus the content
should be taken into account in the retrieval process. We separate
between the structure-based and the content-based retrieval processes.
The content-based score of each node is commonly based on the
well-known Tf × Idf criteria. In this paper, we compare between
this criteria and another one we call Tf × Ief. The comparison
is based on some experiments into a dataset provided by INEX1 to
show the effectiveness of our approach on one hand and those of
both weighting functions on the other.
Abstract: In this paper, we propose a fixed formatting method of PPX(Pretty Printer for XML). PPX is a query language for XML database which has extensive formatting capability that produces HTML as the result of a query. The fixed formatting method is to completely specify the combination of variables and layout specification operators within the layout expression of the GENERATE clause of PPX. In the experiment, a quick comparison shows that PPX requires far less description compared to XSLT or XQuery programs doing the same tasks.
Abstract: IEEE 802.15.4a impulse radio-time hopping ultra wide
band (IR-TH UWB) physical layer, due to small duty cycle and very
short pulse widths is robust against multipath propagation. However,
scattering and reflections with the large number of obstacles in indoor
channel environments, give rise to dense multipath fading. It imposes
serious problem to optimum Rake receiver architectures, for which
very large number of fingers are needed. Presence of strong noise
also affects the reception of fine pulses having extremely low power
spectral density. A robust SRake receiver for IEEE 802.15.4a IRTH
UWB in dense multipath and additive white Gaussian noise
(AWGN) is proposed to efficiently recover the weak signals with
much reduced complexity. It adaptively increases the signal to noise
(SNR) by decreasing noise through a recursive least square (RLS)
algorithm. For simulation, dense multipath environment of IEEE
802.15.4a industrial non line of sight (NLOS) is employed. The power
delay profile (PDF) and the cumulative distribution function (CDF)
for the respective channel environment are found. Moreover, the error
performance of the proposed architecture is evaluated in comparison
with conventional SRake and AWGN correlation receivers. The
simulation results indicate a substantial performance improvement
with very less number of Rake fingers.
Abstract: The counter flow solar air heaters, with four
transverse fins and wire mesh layers are constructed and investigated
experimentally for thermal efficiency at a geographic location of
Cyprus in the city of Famagusta. The absorber plate is replaced by
sixteen steel wire mesh layers, 0.18 x 0.18cm in cross section
opening and a 0.02cm in diameter. The wire mesh layers arranged in
three groups, first and second include 6 layers, while the third include
4 layers. All layers fixed in the duct parallel to the glazing and each
group separated from the others by wood frame thickness of 0.5cm to
reduce the pressure drop. The transverse fins arranged in a way to
force the air to flow through the bed like eight letter path with flow
depth 3cm. The proposed design has increased the heat transfer rate,
but on other hand causes a high pressure drop. The obtained results
show that, for air mass flow rate range between 0.011-0.036kg/s, the
thermal efficiency increases with increasing the air mass flow. The
maximum efficiency obtained is 65.6% for the mass flow rate of
0.036kg/s. Moreover, the temperature difference between the outlet
flow and the ambient temperature, ΔT, reduces as the air mass flow
rate increase. The maximum difference between the outlet and
ambient temperature obtained was 43°C for double pass for minimum
mass flow rate of 0.011kg/s. Comparison with a conventional solar
air heater collector shows a significantly development in the thermal
efficiency.
Abstract: Tasks of the work were study the possible E.coli
contamination in red deer meat, identify pathogenic strains from
isolated E.coli, determine their incidence in red deer meat and
determine the presence of VT1, VT2 and eaeA genes for the
pathogenic E.coli. 8 (10%) samples were randomly selected from 80
analysed isolates of E.coli and PCR reaction was performed on them.
PCR was done both on initial materials – samples of red deer meat -
and for already isolated liqueurs. Two of analysed venison samples
contain verotoxin-producing strains of E. coli. It means that this meat
is not safe to consumer. It was proven by the sequestration reaction of
E. coli and by comparison of the obtained results with the database of
microorganism genome available on the internet that the isolated
culture corresponds to region 16S rDNS of E. coli thus presenting
correctness of the microbiological methods.
Abstract: The changing economic climate has made global
manufacturing a growing reality over the last decade, forcing
companies from east and west and all over the world to
collaborate beyond geographic boundaries in the design,
manufacture and assemble of products. The ISO10303 and
ISO14649 Standards (STEP and STEP-NC) have been
developed to introduce interoperability into manufacturing
enterprises so as to meet the challenge of responding to
production on demand. This paper describes and illustrates a
STEP compliant CAD/CAPP/CAM System for the manufacture
of rotational parts on CNC turning centers. The information
models to support the proposed system together with the data
models defined in the ISO14649 standard used to create the NC
programs are also described. A structured view of a STEP
compliant CAD/CAPP/CAM system framework supporting the
next generation of intelligent CNC controllers for turn/mill
component manufacture is provided. Finally a proposed
computational environment for a STEP-NC compliant system
for turning operations (SCSTO) is described. SCSTO is the
experimental part of the research supported by the specification
of information models and constructed using a structured
methodology and object-oriented methods. SCSTO was
developed to generate a Part 21 file based on machining
features to support the interactive generation of process plans
utilizing feature extraction. A case study component has been
developed to prove the concept for using the milling and turning
parts of ISO14649 to provide a turn-mill CAD/CAPP/CAM
environment.
Abstract: The paper structures research approaches to the crisis
and its management. It focuses on approaches – psychological,
sociological, economic, ethical and technological. Furthermore, it
describes the basic features of models chosen according to those
approaches. By their comparison it shows how the crisis influences
organizations and individuals, and their mutual interaction.
Abstract: To improve the dynamics response of the vehicle
passive suspension, a two-terminal mass is suggested to connect in
parallel with the suspension strut. Three performance criteria, tire grip,
ride comfort and suspension deflection, are taken into consideration to
optimize the suspension parameters. However, the three criteria are
conflicting and non-commensurable. For this reason, the Chebyshev
goal programming method is applied to find the best tradeoff among
the three objectives. A simulation case is presented to describe the
multi-objective optimization procedure. For comparison, the
Chebyshev method is also employed to optimize the design of a
conventional passive suspension. The effectiveness of the proposed
design method has been clearly demonstrated by the result. It is also
shown that the suspension with a two-terminal mass in parallel has
better performance in terms of the three objectives.
Abstract: Mobile WiMAX is a broadband wireless solution that
enables convergence of mobile and fixed broadband networks
through a common wide area broadband radio access technology and
flexible network architecture. It adopts Orthogonal Frequency
Division Multiple Access (OFDMA) for improved multi-path
performance in Non-Line-Of-Sight (NLOS) environments. Scalable
OFDMA (SOFDMA) is introduced in the IEEE 802e[1]. WIMAX
system uses one of different types of channel coding but The
mandatory channel coding scheme is based on binary nonrecursive
Convolutional Coding (CC). There are other several optional channel
coding schemes such as block turbo codes, convolutional turbo
codes, and low density parity check (LDPC).
In this paper a comparison between the performance of WIMAX
using turbo code and using convolutional product code (CPC) [2] is
made. Also a combination between them had been done. The CPC
gives good results at different SNR values compared to both the
turbo system, and the combination between them. For example, at
BER equal to 10-2 for 128 subcarriers, the amount of improvement
in SNR equals approximately 3 dB higher than turbo code and equals
approximately 2dB higher than the combination respectively. Several
results are obtained at different modulating schemes (16QAM and
64QAM) and different numbers of sub-carriers (128 and 512).
Abstract: In this paper the effect of faults in the elements and
parts of discrete event systems is investigated. In the occurrence of
faults, some states of the system must be changed and some of them
must be forbidden. For this goal, different states of these elements are
examined and a model for fail-safe behavior of each state is
introduced. Replacing new models of the target elements in the
preliminary model by a systematic method, leads to a fail-safe
discrete event system.
Abstract: The characteristic bending strength (MOR) and mean
modulus of elasticity (MOE) of tropical hardwood red seraya (Shorea
spp.) plywood were determined using European Standard EN310 and
EN789. The thickness of the test specimen was 4.0mm, 7.0mm,
9.0mm, 12.0mm and 15.0mm. The experiment found that the MOR
of red seraya plywood in EN310 is about 12% to 20% and 7% to
24% higher than EN789 whereas MOE were about 28% to 41% and
30% to 36% lower than those obtained from EN 789 for test
specimens parallel and perpendicular to the grain direction. The
linear regression shows that MOR and MOE for EN789 is about 0.8
times less and 1.5 times more than EN310. The experiment also
found that the MOR and MOE of EN310 and EN789 also depend on
the wood species that used in the experiment.
Abstract: Von Willebrand-s disease is the most common
inherited bleeding disorder in humans, it
caused by qualitative abnormalities of the von Willebrand factor
(vWF). Our objective is to determine the prevalence of this disease at
part of the Algerian population in the East and the South by a
biological diagnosis based on specific biological tests (automated
platelet count, the bleeding time (TS), the time of cephalin + activator
(TCA), measure of the prothrombin rate (TP), vWF rate and factor
VIII rate, Molecular electrophoresis of vWF multimers in agarose gel
in the presence of SDS). Four patients of type III or severe
Willebrand-s disease were found on 200 suspect cases. All cases are
showed a deficit in vWF rate (< 5%), and factor VIII (P
Abstract: This paper presents a modified version of the
maximum urgency first scheduling algorithm. The maximum
urgency algorithm combines the advantages of fixed and dynamic
scheduling to provide the dynamically changing systems with
flexible scheduling. This algorithm, however, has a major
shortcoming due to its scheduling mechanism which may cause a
critical task to fail. The modified maximum urgency first scheduling
algorithm resolves the mentioned problem. In this paper, we propose
two possible implementations for this algorithm by using either
earliest deadline first or modified least laxity first algorithms for
calculating the dynamic priorities. These two approaches are
compared together by simulating the two algorithms. The earliest
deadline first algorithm as the preferred implementation is then
recommended. Afterwards, we make a comparison between our
proposed algorithm and maximum urgency first algorithm using
simulation and results are presented. It is shown that modified
maximum urgency first is superior to maximum urgency first, since it
usually has less task preemption and hence, less related overhead. It
also leads to less failed non-critical tasks in overloaded situations.
Abstract: The paper presents a set of guidelines for analysis of industrial embedded distributed systems and introduces a mathematical model derived from these guidelines. In this study, the author examines a set of modern communication technologies that are or possibly can be used to build communication links between the subsystems of a distributed embedded system. An investigation of these guidelines results in a algorithm for analysis of specific use cases of target technologies. A goal of the paper acts as an important base for ongoing research on comparison of communication technologies. The author describes the principles of the model and presents results of the test calculations. Practical implementation of target technologies and empirical experiment data are based on a practical experience during the design and test of specific distributed systems in Latvian market.
Abstract: Each new semiconductor technology node
brings smaller transistors and wires. Although this makes
transistors faster, wires get slower. In nano-scale regime, the
standard copper (Cu) interconnect will become a major hurdle
for FPGA interconnect due to their high resistivity and
electromigration. This paper presents the comprehensive
evaluation of mixed CNT bundle interconnects and
investigates their prospects as energy efficient and high speed
interconnect for future FPGA routing architecture. All
HSPICE simulations are carried out at operating frequency of
1GHz and it is found that mixed CNT bundle implemented in
FPGAs as interconnect can potentially provide a substantial
delay and energy reduction over traditional interconnects at
32nm process technology.