Abstract: Chemical reaction and diffusion are important phenomena in quantitative neurobiology and biophysics. The knowledge of the dynamics of calcium Ca2+ is very important in cellular physiology because Ca2+ binds to many proteins and regulates their activity and interactions Calcium waves propagate inside cells due to a regenerative mechanism known as calcium-induced calcium release. Buffer-mediated calcium diffusion in the cytosol plays a crucial role in the process. A mathematical model has been developed for calcium waves by assuming the buffers are in equilibrium with calcium i.e., the rapid buffering approximation for a one dimensional unsteady state case. This model incorporates important physical and physiological parameters like dissociation rate, diffusion rate, total buffer concentration and influx. The finite difference method has been employed to predict [Ca2+] and buffer concentration time course regardless of the calcium influx. The comparative studies of the effect of the rapid buffered diffusion and kinetic parameters of the model on the concentration time course have been performed.
Abstract: Blood pulse is an important human physiological signal commonly used for the understanding of the individual physical health. Current methods of non-invasive blood pulse sensing require direct contact or access to the human skin. As such, the performances of these devices tend to vary with time and are subjective to human body fluids (e.g. blood, perspiration and skin-oil) and environmental contaminants (e.g. mud, water, etc). This paper proposes a simulation model for the novel method of non-invasive acquisition of blood pulse using the disturbance created by blood flowing through a localized magnetic field. The simulation model geometry represents a blood vessel, a permanent magnet, a magnetic sensor, surrounding tissues and air in 2-dimensional. In this model, the velocity and pressure fields in the blood stream are described based on Navier-Stroke equations and the walls of the blood vessel are assumed to have no-slip condition. The blood assumes a parabolic profile considering a laminar flow for blood in major artery near the skin. And the inlet velocity follows a sinusoidal equation. This will allow the computational software to compute the interactions between the magnetic vector potential generated by the permanent magnet and the magnetic nanoparticles in the blood. These interactions are simulated based on Maxwell equations at the location where the magnetic sensor is placed. The simulated magnetic field at the sensor location is found to assume similar sinusoidal waveform characteristics as the inlet velocity of the blood. The amplitude of the simulated waveforms at the sensor location are compared with physical measurements on human subjects and found to be highly correlated.
Abstract: Physiological control of a left ventricle assist device (LVAD) is generally a complicated task due to diverse operating environments and patient variability. In this work, a tracking control algorithm based on sliding mode and feed forward control for a class of discrete-time single input single output (SISO) nonlinear uncertain systems is presented. The controller was developed to track the reference trajectory to a set operating point without inducing suction in the ventricle. The controller regulates the estimated mean pulsatile flow Qp and mean pulsatility index of pump rotational speed PIω that was generated from a model of the assist device. We recall the principle of the sliding mode control theory then we combine the feed-forward control design with the sliding mode control technique to follow the reference trajectory. The uncertainty is replaced by its upper and lower boundary. The controller was tested in a computer simulation covering two scenarios (preload and ventricular contractility). The simulation results prove the effectiveness and the robustness of the proposed controller
Abstract: The present study aimed to investigate whether
chlorophyll meter readings (SPAD) can be used as criterion of singleplant
selection in maize breeding. Experimentation was performed at
the ultra-low density of 0.74 plants/m2 in order the potential yield per
plant to be fully expressed. R-31 honeycomb experiments were
conducted in three different areas in Greece (Thessaloniki, Giannitsa
and Florina) using 30 inbred lines at well-watered and water-stressed
conditions during the 2012 growing season. The chlorophyll meter
readings had higher rates at dry conditions, except location of
Giannitsa where differences were not significant. Genotypes of
highest chlorophyll meter readings were consistent across areas,
emphasizing on the character’s stability. A positive correlation
between the chlorophyll meter readings and grain yield was
strengthening over time and culminated at the physiological maturity
stage. There was a clear sign that the chlorophyll meter readings has
the potential to be used for the selection of stress-adaptive genotypes
and may permit modern maize to be grown at wider range of
environments addressing the climate change scenarios.
Abstract: Electrophysiological signals were recorded from primary cultures of dissociated rat cortical neurons coupled to Micro-Electrode Arrays (MEAs). The neuronal discharge patterns may change under varying physiological and pathological conditions. For this reason, we developed a new burst detection method able to identify bursts with peculiar features in different experimental conditions (i.e. spontaneous activity and under the effect of specific drugs). The main feature of our algorithm (i.e. Burst On Hurst), based on the auto-similarity or fractal property of the recorded signal, is the independence from the chosen spike detection method since it works directly on the raw data.
Abstract: This study aims to demonstrate the quantification of
peptides based on isotope dilution surface enhanced Raman
scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine
(Leu) and two peptide sequences TGQIFK (T13) and
YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa
human growth hormone (hGH) were obtained on Ag-nanoparticle
covered substrates. On the basis of the dominant Phe and Leu
vibrational modes, precise partial least squares (PLS) prediction
models were built enabling the determination of unknown T13 and
T6 concentrations. Detection of hGH in its physiological
concentration in order to investigate the possibility of protein
quantification has been achieved.
Abstract: Diagnosis can be achieved by building a model of a
certain organ under surveillance and comparing it with the real time
physiological measurements taken from the patient. This paper deals
with the presentation of the benefits of using Data Mining techniques
in the computer-aided diagnosis (CAD), focusing on the cancer
detection, in order to help doctors to make optimal decisions quickly
and accurately. In the field of the noninvasive diagnosis techniques,
the endoscopic ultrasound elastography (EUSE) is a recent elasticity
imaging technique, allowing characterizing the difference between
malignant and benign tumors. Digitalizing and summarizing the main
EUSE sample movies features in a vector form concern with the use
of the exploratory data analysis (EDA). Neural networks are then
trained on the corresponding EUSE sample movies vector input in
such a way that these intelligent systems are able to offer a very
precise and objective diagnosis, discriminating between benign and
malignant tumors. A concrete application of these Data Mining
techniques illustrates the suitability and the reliability of this
methodology in CAD.
Abstract: In ubiqutious healthcare environment, user's health data are transfered to the remote healthcare server by the user's wearable system or mobile phone. These collected user's health data should be managed and analyzed in the healthcare server, so that care giver or user can monitor user's physiological state. In this paper, we designed and developed the intelligent Healthcare Server to manage the user's health data using CDSS and ontology. Our system can analyze user's health data semantically using CDSS and ontology, and report the result of user's physiological raw data to the user and care giver.
Abstract: Biochemical Oxygen Demand (BOD) is a measure of
the oxygen used in bacteria mediated oxidation of organic substances
in water and wastewater. Theoretically an infinite time is required for
complete biochemical oxidation of organic matter, but the
measurement is made over 5-days at 20 0C or 3-days at 27 0C test
period with or without dilution. Researchers have worked to further
reduce the time of measurement.
The objective of this paper is to review advancement made in
BOD measurement primarily to minimize the time and negate the
measurement difficulties. Survey of literature review in four such
techniques namely BOD-BARTTM, Biosensors, Ferricyanidemediated
approach, luminous bacterial immobilized chip method.
Basic principle, method of determination, data validation and their
advantage and disadvantages have been incorporated of each of the
methods.
In the BOD-BARTTM method the time lag is calculated for the
system to change from oxidative to reductive state. BIOSENSORS
are the biological sensing element with a transducer which produces
a signal proportional to the analyte concentration. Microbial species
has its metabolic deficiencies. Co-immobilization of bacteria using
sol-gel biosensor increases the range of substrate. In ferricyanidemediated
approach, ferricyanide has been used as e-acceptor instead
of oxygen. In Luminous bacterial cells-immobilized chip method,
bacterial bioluminescence which is caused by lux genes was
observed. Physiological responses is measured and correlated to
BOD due to reduction or emission.
There is a scope to further probe into the rapid estimation of BOD.
Abstract: The experiment was performed to evaluate the effect
of GA3, 2,4-D on fruit growth and fruit quality of wax apple. The
experiment consisted of Red A, Monulla, Atu, Red B cultivars. GA3
and 2,4-D were applied at the small bud and petal fall stage.
Physiological, biochemical characters of fruit were recoded. The
result showed application of GA3, 2,4-D greatly response in
increasing fruit set for all treatment as compared to control. Fruit
weight, fruit size were increased at 10 ppm 2,4-D in ‘Red A’, ‘Red
B’, however it was also enhancing at 10 ppm GA3 in ‘Monulla’,
‘Atu’. For ‘Monulla’, ‘Atu’ fruit crack reduced by 10 ppm 2,4-D
application, but ‘Red B’, ‘Red A’ gave least fruit crack at 10 and 30
ppm GA3, respectively. ‘Monulla’, ‘Atu’ and ‘Red B’ resulted in
response well to 10 ppm GA3 on improving TSS, whereas
application of 30 ppm GA3 greatly enhancing TSS in ‘Red A’. For
‘Atu’ titratable acidity markedly reduced by 10 ppm GA3
application, but spraying with 30 ppm GA3 greatly response in
reducing titratable acidity in ‘Red A’, ‘Red B’ and ‘Monulla’. It was
concluded that GA3, 2,4-D can be an effective tool to enhancing fruit
set, fruit growth as well as improving fruit quality of wax apple.
Abstract: The effect of shearing date on behaviors and performances of 20 pregnant Rahmani ewes was evaluated in four groups (5each). Ewes were shorn at 70, 100 and 130 days of pregnancy in the first three groups respectively, while the fourth group was maintained unshorn as a control. Some behavioral and physiological data related to ewes in addition, blood cortisol level were recorded. Results revealed a significant increase in the frequencies of comfort and eating behaviors, respiratory rate, pulse rate, lamb birth weight and blood cortisol level in early and mid pregnancy shorn ewes. Also, a slight increase in pregnancy period was observed for those ewes. On the other hand, social behaviors, and core temperature were not affected by shearing. These results conclude that prenatal shearing (early and mid-pregnancy) of ewes increases the frequencies of comfort and eating behaviors, and improves the survival rates of lambs by increasing their birth weights.
Abstract: One field experiment was conducted on corn (Zea
mays L.Var. SC 704) to study the effect of three different basic levels
of nitrogen (90, 140and 190 Kg/ha as urea) with 0.01% and 0.02%
pyridoxine pre-sowing seed soaking for 8 hours. Water-soaked seeds
were treated as controled. biomass production was recorded on 45,
70 and 95 days after sowing. Total dry material (TDM), leaf area
index (LAI), crop growth rate (CGR), relative growth rate (RGR) and
net assimilation rate (NAR) was calculated form 45until 95 days after
sowing. Yield and its components such as kernel yield, grain weight,
biologic yield, harvest index and protein percentage was measured at
harvest. In general, 0.02% pyridoxine and 190 Kg pure nitrogen/ha
was shown gave maximum value for growth and yield parameters.
N190 + 0.02 % pyridoxine enhanced seed yield and biologic yield by
57.15% and 62.98% compared to 90kg N and water – soaked
treatment.
Abstract: Fake finger submission attack is a major problem in fingerprint recognition systems. In this paper, we introduce an aliveness detection method based on multiple static features, which derived from a single fingerprint image. The static features are comprised of individual pore spacing, residual noise and several first order statistics. Specifically, correlation filter is adopted to address individual pore spacing. The multiple static features are useful to reflect the physiological and statistical characteristics of live and fake fingerprint. The classification can be made by calculating the liveness scores from each feature and fusing the scores through a classifier. In our dataset, we compare nine classifiers and the best classification rate at 85% is attained by using a Reduced Multivariate Polynomial classifier. Our approach is faster and more convenient for aliveness check for field applications.
Abstract: The beneficial effects of Si are mainly associated with
its high deposition in plant tissue and enhancing their strength and
rigidity. We investigated the role of Si against cadmium stress in
(Echium C) in house green condition. When the seventh leaves was
be appeared, plants were pretreated with five levels of Si: 0, 0.2, 0.5,
0.7and 1.5 mM Si (as sodium trisilicate, Na2(SiO2)3) and after that
plants were treated with two levels of Cd (30 and 90 mM). The
effects of Silicon and Cd were investigated on some physiological
and biochemical parameters such as: lipid peroxidation
(malondialdehyde (MDA) and other aldehydes, antocyanin and
flavonoid content. Our results showed that Cd significantly increased
MDA, other aldehydes, antocyanin and flavonoids content in
Echium and silicon offset the negative effect and increased tolerance
of Echium against Cd stress. From this results we concluded that Si
increase membrane integrity and antioxidative ability in this plant
against cd stress.
Abstract: This paper proposes a novel architecture for At-
Home medical care which enables senior citizens, patients
with chronic ailments and patients requiring post- operative
care to be remotely monitored in the comfort of their homes.
This architecture is implemented using sensors and wireless
networking for transmitting patient data to the hospitals,
health- care centers for monitoring by medical professionals.
Patients are equipped with sensors to measure their
physiological parameters, like blood pressure, pulse rate etc.
and a Wearable Data Acquisition Unit is used to transmit the
patient sensor data. Medical professionals can be alerted to
any abnormal variations in these values for diagnosis and
suitable treatment. Security threats and challenges inherent to
wireless communication and sensor network have been
discussed and a security mechanism to ensure data
confidentiality and source authentication has been proposed.
Symmetric key algorithm AES has been used for encrypting
the data and a patent-free, two-pass block cipher mode CCFB
has been used for implementing semantic security.
Abstract: Compensating physiological motion in the context
of minimally invasive cardiac surgery has become an attractive
issue since it outperforms traditional cardiac procedures offering
remarkable benefits. Owing to space restrictions, computer vision
techniques have proven to be the most practical and suitable solution.
However, the lack of robustness and efficiency of existing methods
make physiological motion compensation an open and challenging
problem. This work focusses on increasing robustness and efficiency
via exploration of the classes of 1−and 2−regularized optimization,
emphasizing the use of explicit regularization. Both approaches are
based on natural features of the heart using intensity information.
Results pointed out the 1−regularized optimization class as the best
since it offered the shortest computational cost, the smallest average
error and it proved to work even under complex deformations.
Abstract: The study describes chitosan membrane platform
modified with nanostructure pattern which using nanotechnology to
fabricate. The cell-substrate interaction between neuro-2a neuroblasts
cell lines and chitosan membrane (flat, nanostructure and
nanostructure pattern types) was investigated. The adhered
morphology of neuro-2a cells depends on the topography of chitosan
surface. We have found that neuro-2a showed different morphogenesis
when cells adhered on flat and nanostructure chitosan membrane. The
cell projected area of neuro-2a on flat chitosan membrane is larger
than on nanostructure chitosan membrane. In addition, neuro-2a cells
preferred to adhere on flat chitosan surface region than on
nanostructure chitosan membrane to immobilize and differentiation.
The experiment suggests surface topography can be used as a critical
mechanism to isolate group of neuro-2a to a particular rectangle area
on chitosan membrane. Our finding will provide a platform to take
patch clamp to record electrophysiological behavior about neurons in
vitro in the future.
Abstract: The C3 plants are frequently suffering from exposure
to high temperature stress which limits the growth and yield of these
plants. This study seeks to clarify the physiological mechanisms of
heat tolerance in relation to oxidative stress in C3 species. Fifteen C3
species were exposed to prolonged moderately high temperature
stress 36/30°C for 40 days in a growth chamber. Chlorophyll
fluorescence (Fv/Fm) showed great difference among species at 40
days of the stress. The species showed decreases in Fv/Fm and
increases in malondialdehyde (MDA) content under stress condition
as well as negative correlation between Fv/Fm and MDA (r = -0.61*)
at 40 days of the stress. Hydrogen peroxide (H2O2) content before
and after stress in addition to its response under stress showed great
differences among species. The results suggest that the difference in
heat tolerance among C3 species is closely associated with the ability
to suppress oxidative damage but not with the content of reactive
oxygen species (ROS) which is regulated by complex network.
Abstract: If a possibility distribution and a probability distribution
are describing values x of one and the same system or process
x(t), can they relate to each other? Though in general the possibility
and probability distributions might be not connected at all, we
can assume that in some particular cases there is an association linked
them.
In the presented paper, we consider distributions of bloodstream
concentrations of physiologically active substances and propose that
the probability to observe a concentration x of a substance X can be
produced from the possibility of the event X = x .
The proposed assumptions and resulted theoretical distributions
are tested against the data obtained from various panel studies of the
bloodstream concentrations of the different physiologically active
substances in patients and healthy adults as well.
Abstract: Recent advancements in sensor technologies and
Wireless Body Area Networks (WBANs) have led to the
development of cost-effective healthcare devices which can be used
to monitor and analyse a person-s physiological parameters from
remote locations. These advancements provides a unique opportunity
to overcome current healthcare challenges of low quality service
provisioning, lack of easy accessibility to service varieties, high costs
of services and increasing population of the elderly experienced
globally. This paper reports on a prototype implementation of an
architecture that seamlessly integrates Wireless Body Area Network
(WBAN) with Web services (WS) to proactively collect
physiological data of remote patients to recommend diagnostic
services. Technologies based upon WBAN and WS can provide
ubiquitous accessibility to a variety of services by allowing
distributed healthcare resources to be massively reused to provide
cost-effective services without individuals physically moving to the
locations of those resources. In addition, these technologies can
reduce costs of healthcare services by allowing individuals to access
services to support their healthcare. The prototype uses WBAN body
sensors implemented on arduino fio platforms to be worn by the
patient and an android smart phone as a personal server. The
physiological data are collected and uploaded through GPRS/internet
to the Medical Health Server (MHS) to be analysed. The prototype
monitors the activities, location and physiological parameters such as
SpO2 and Heart Rate of the elderly and patients in rehabilitation.
Medical practitioners would have real time access to the uploaded
information through a web application.