Abstract: Gene expression profiling is rapidly evolving into a
powerful technique for investigating tumor malignancies. The
researchers are overwhelmed with the microarray-based platforms
and methods that confer them the freedom to conduct large-scale
gene expression profiling measurements. Simultaneously,
investigations into cross-platform integration methods have started
gaining momentum due to their underlying potential to help
comprehend a myriad of broad biological issues in tumor diagnosis,
prognosis, and therapy. However, comparing results from different
platforms remains to be a challenging task as various inherent
technical differences exist between the microarray platforms. In this
paper, we explain a simple ratio-transformation method, which can
provide some common ground for cDNA and Affymetrix platform
towards cross-platform integration. The method is based on the
characteristic data attributes of Affymetrix- and cDNA- platform. In
the work, we considered seven childhood leukemia patients and their
gene expression levels in either platform. With a dataset of 822
differentially expressed genes from both these platforms, we carried
out a specific ratio-treatment to Affymetrix data, which subsequently
showed an improvement in the relationship with the cDNA data.
Abstract: We evaluated the effect of sensory (direct current
(DC), 600μA) and motor (monophasic current, pulse duration 300μs,
100 Hz, 2.5-3mA) intensities of cathodal electrical stimulation (ES)
current to release VEGF and biomechanical properties of wound. 54
male Sprague-dawley rats were randomly assigned into one control
and two experimental groups. A full thickness skin incision was
made on animals- dorsal region. The experimental groups received
ES for 1h/day and every other day. VEGF expression was measured
in skin on the 7th day after surgical incision and tensile strength was
measured on 21st day. On the 7th day, the values of skin VEGF in the
sensory group were significantly greater than those of the other
groups (p < 0.05). Sensory and Motor intensity stimulation, can not
improve the biomechanical properties of the repaired wounds.
It seems the mechanical environment induced by sensory and
motor intensity of electrical stimulation, could not simulate the role
of normal daily stress and strain to maturation of collagen fibers and
their cross links. Further work is needed to determine the relationship
between VEGF expression after ES and its effect on tensile strength
of healed wound.
Abstract: Heart-s electric field can be measured anywhere on
the surface of the body (ECG). When individuals touch, one person-s
ECG signal can be registered in other person-s EEG and elsewhere
on his body. Now, the aim of this study was to test the hypothesis
that physical contact (hand-holding) of two persons changes their
heart rate variability. Subjects were sixteen healthy female (age: 20-
26) which divided into eight sets. In each sets, we had two friends
that they passed intimacy test of J.sternberg. ECG of two subjects
(each set) acquired for 5 minutes before hand-holding (as control
group) and 5 minutes during they held their hands (as experimental
group). Then heart rate variability signals were extracted from
subjects' ECG and analyzed in linear feature space (time and
frequency domain) and nonlinear feature space. Considering the
results, we conclude that physical contact (hand-holding of two
friends) increases parasympathetic activity, as indicate by increase
SD1, SD1/SD2, HF and MF power (p
Abstract: Acute kidney injury (AKI) is a new worldwide public
health problem. A diagnosis of this disease using creatinine is still a
problem in clinical practice. Therefore, a measurement of biomarkers
responsible for AKI has received much attention in the past couple
years. Cytokine interleukin-18 (IL-18) was reported as one of the
early biomarkers for AKI. The most commonly used method to
detect this biomarker is an immunoassay. This study used a planar
platform to perform an immunoassay using fluorescence for
detection. In this study, anti-IL-18 antibody was immobilized onto a
microscope slide using a covalent binding method. Make-up samples
were diluted at the concentration between 10 to 1000 pg/ml to create
a calibration curve. The precision of the system was determined
using a coefficient of variability (CV), which was found to be less
than 10%. The performance of this immunoassay system was
compared with the measurement from ELISA.
Abstract: This paper describes an automatic algorithm to restore
the shape of three-dimensional (3D) left ventricle (LV) models created
from magnetic resonance imaging (MRI) data using a geometry-driven
optimization approach. Our basic premise is to restore the LV shape
such that the LV epicardial surface is smooth after the restoration. A
geometrical measure known as the Minimum Principle Curvature (κ2)
is used to assess the smoothness of the LV. This measure is used to
construct the objective function of a two-step optimization process.
The objective of the optimization is to achieve a smooth epicardial
shape by iterative in-plane translation of the MRI slices.
Quantitatively, this yields a minimum sum in terms of the magnitude
of κ
2, when κ2 is negative. A limited memory quasi-Newton algorithm,
L-BFGS-B, is used to solve the optimization problem. We tested our
algorithm on an in vitro theoretical LV model and 10 in vivo
patient-specific models which contain significant motion artifacts. The
results show that our method is able to automatically restore the shape
of LV models back to smoothness without altering the general shape of
the model. The magnitudes of in-plane translations are also consistent
with existing registration techniques and experimental findings.
Abstract: Hysitron TriboIndenterTM TI 950 system has been
used for studying the local viscoelastic properties of porcine
intervertebral disc end plate by means of nanoscale mechanical
dynamic analysis. The specimen of an endplate was cut from fresh
porcine vertebra dissected from 16 month animal. The lumbar spine
motion segments were dissected and 5 millimeter thick plates of
vertebral body, endplate and annulus fibrosus were prepared for
nanoindentation. The surface of the sample was kept in physiological
solution during nanoindentation experiment. We obtained mechanical
characteristics of different areas of native endplate (endplate middle
and vertebra and annulus fibrosus boundary).
Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
Abstract: Retinal vascularity assessment plays an important role in diagnosis of ophthalmic pathologies. The employment of digital images for this purpose makes possible a computerized approach and has motivated development of many methods for automated vascular tree segmentation. Metrics based on contingency tables for binary classification have been widely used for evaluating performance of these algorithms and, concretely, the accuracy has been mostly used as measure of global performance in this topic. However, this metric shows very poor matching with human perception as well as other notable deficiencies. Here, a new similarity function for measuring quality of retinal vessel segmentations is proposed. This similarity function is based on characterizing the vascular tree as a connected structure with a measurable area and length. Tests made indicate that this new approach shows better behaviour than the current one does. Generalizing, this concept of measuring descriptive properties may be used for designing functions for measuring more successfully segmentation quality of other complex structures.
Abstract: The standard investigational method for obstructive
sleep apnea syndrome (OSAS) diagnosis is polysomnography (PSG),
which consists of a simultaneous, usually overnight recording of
multiple electro-physiological signals related to sleep and
wakefulness. This is an expensive, encumbering and not a readily
repeated protocol, and therefore there is need for simpler and easily
implemented screening and detection techniques. Identification of
apnea/hypopnea events in the screening recordings is the key factor
for the diagnosis of OSAS. The analysis of a solely single-lead
electrocardiographic (ECG) signal for OSAS diagnosis, which may
be done with portable devices, at patient-s home, is the challenge of
the last years. A novel artificial neural network (ANN) based
approach for feature extraction and automatic identification of
respiratory events in ECG signals is presented in this paper. A
nonlinear principal component analysis (NLPCA) method was
considered for feature extraction and support vector machine for
classification/recognition. An alternative representation of the
respiratory events by means of Kohonen type neural network is
discussed. Our prospective study was based on OSAS patients of the
Clinical Hospital of Pneumology from Iaşi, Romania, males and
females, as well as on non-OSAS investigated human subjects. Our
computed analysis includes a learning phase based on cross signal
PSG annotation.
Abstract: The technological concepts such as wireless hospital
and portable cardiac telemetry system require the development of
physiological signal acquisition devices to be easily integrated into
the hospital database. In this paper we present the low cost, portable
wireless ECG acquisition hardware that transmits ECG signals to a
dedicated computer.The front end of the system obtains and
processes incoming signals, which are then transmitted via a
microcontroller and wireless Bluetooth module. A monitoring
purpose Bluetooth based end user application integrated with patient
database management module is developed for the computers. The
system will act as a continuous event recorder, which can be used to
follow up patients who have been resuscitatedfrom cardiac arrest,
ventricular tachycardia but also for diagnostic purposes for patients
with arrhythmia symptoms. In addition, cardiac information can be
saved into the patient-s database of the hospital.
Abstract: Interventional cardiologists are at greater risk from
radiation exposure as a result of the procedures they undertake than
most other medical specialists. A study was performed to evaluate
operator dose during interventional cardiology procedures and to
establish methods of operator dose reduction with a radiation
protective device. Different procedure technique and use of
protective tools can explain big difference in the annual equivalent
dose received by the professionals. Strategies to prevent and
monitor radiation exposure, advanced protective shielding and
effective radiation monitoring methods should be applied.
Abstract: The Brazilian legislation has only established
diagnostic reference levels (DRLs) in terms of Multiple Scan
Average Dose (MSAD) as a quality control parameter for computed
tomography (CT) scanners. Compliance with DRLs can be verified
by measuring the Computed Tomography Kerma Index (Ca,100) with
a pencil ionization chamber or by obtaining the kerma distribution in
CT scans with radiochromic films or rod shape lithium fluoride
termoluminescent dosimeters (TLD-100). TL dosimeters were used
to record kerma profiles and to determine MSAD values of a Bright
Speed model GE CT scanner. Measurements were done with
radiochromic films and TL dosimeters distributed in cylinders
positioned in the center and in four peripheral bores of a standard
polymethylmethacrylate (PMMA) body CT dosimetry phantom.
Irradiations were done using a protocol for adult chest. The
maximum values were found at the midpoint of the longitudinal axis.
The MSAD values obtained with three dosimetric techniques were
compared.
Abstract: The processing of the electrocardiogram (ECG) signal consists essentially in the detection of the characteristic points of
signal which are an important tool in the diagnosis of heart diseases. The most suitable are the detection of R waves. In this paper, we
present various mathematical tools used for filtering ECG using digital filtering and Discreet Wavelet Transform (DWT) filtering. In
addition, this paper will include two main R peak detection methods
by applying a windowing process: The first method is based on calculations derived, the second is a time-frequency method based on
Dyadic Wavelet Transform DyWT.
Abstract: Noise level has critical effects on the diagnostic
performance of signal-averaged electrocardiogram (SAECG), because
the true starting and end points of QRS complex would be masked by
the residual noise and sensitive to the noise level. Several studies and
commercial machines have used a fixed number of heart beats
(typically between 200 to 600 beats) or set a predefined noise level
(typically between 0.3 to 1.0 μV) in each X, Y and Z lead to perform
SAECG analysis. However different criteria or methods used to
perform SAECG would cause the discrepancies of the noise levels
among study subjects. According to the recommendations of 1991
ESC, AHA and ACC Task Force Consensus Document for the use of
SAECG, the determinations of onset and offset are related closely to
the mean and standard deviation of noise sample. Hence this study
would try to perform SAECG using consistent root-mean-square
(RMS) noise levels among study subjects and analyze the noise level
effects on SAECG. This study would also evaluate the differences
between normal subjects and chronic renal failure (CRF) patients in
the time-domain SAECG parameters.
The study subjects were composed of 50 normal Taiwanese and 20
CRF patients. During the signal-averaged processing, different RMS
noise levels were adjusted to evaluate their effects on three time
domain parameters (1) filtered total QRS duration (fQRSD), (2) RMS
voltage of the last QRS 40 ms (RMS40), and (3) duration of the low
amplitude signals below 40 μV (LAS40). The study results
demonstrated that the reduction of RMS noise level can increase
fQRSD and LAS40 and decrease the RMS40, and can further increase
the differences of fQRSD and RMS40 between normal subjects and
CRF patients. The SAECG may also become abnormal due to the
reduction of RMS noise level. In conclusion, it is essential to establish
diagnostic criteria of SAECG using consistent RMS noise levels for
the reduction of the noise level effects.
Abstract: Breast cancer is the most common malignancy in the
world among women. Many therapies have been designed to treat
this disease. Mamectomy, chemotherapy and radiotherapy are still
the main therapies of breast cancer. However, the results were
unsatisfactory and still far from the ideal treatment.
PM 701is a natural product, has anticancer activity. The bioactive
fraction PMF and subfraction PMFK had been isolated from PM701.
PM 701 and its fractions were proved to have a cytotoxic properties
against different cancer cell lines. This article is directed for the
further examination of lyophilized PM701 and its active fractions on
the growth of breast cancer cells (MCF-7). PM 701, PMF or PMFK
were adding to the cultural medium, where MCF-7 is incubated.
PM 701, PMF or PMFK were able to inhibit significantly the
proliferation of MCF-7 cells, Moreover these new agents were
proved to induce apoptosis of the breast cancer cells; through its
direct effect on the nuclei.
Abstract: Automatic determination of blood in less bright or
noisy capsule endoscopic images is difficult due to low S/N ratio.
Especially it may not be accurate to analyze these images due to the
influence of external disturbance. Therefore, we proposed detection
methods that are not dependent only on color bands. In locating
bleeding regions, the identification of object outlines in the frame and
features of their local colors were taken into consideration. The results
showed that the capability of detecting bleeding was much improved.
Abstract: The article reviews the current state of large-scale
studies about the impact of electromagnetic field on natural
environment. The scenario of investigations – simulation of natural
conditions at the workplace, taking into consideration the influence
both low and high frequency electromagnetic fields is shown.The
biological effects of low and high frequency electromagnetic fields
are below presented. Results of investigation with animals are shown.
The norms and regulations concerning the levels of electromagnetic
field intensity are reviewed.
Abstract: The focal spot of a high intensity focused ultrasound
transducer is small. To heat a large target volume, multiple treatment spots are required. If the power of each treatment spot is fixed, it could
results in insufficient heating of initial spots and over-heating of later ones, which is caused by the thermal diffusion. Hence, to produce a
uniform heated volume, the delivered energy of each treatment spot
should be properly adjusted. In this study, we proposed an iterative, extrapolation technique to adjust the required ultrasound energy of
each treatment spot. Three different scanning pathways were used to evaluate the performance of this technique. Results indicate that by using the proposed technique, uniform heating volume could be obtained.
Abstract: Image segmentation is an important step in image
processing. Major developments in medical imaging allow
physicians to use potent and non-invasive methods in order to
evaluate structures, performance and to diagnose human diseases. In
this study, an active contour was used to extract vessel networks
from color retina images. Automatic analysis of retina vessels
facilitates calculation of arterial index which is required to diagnose
some certain retinopathies.
Abstract: In this paper we introduce a novel method for
the characterization of synchronziation and coupling effects
in multivariate time series that can be used for the analysis
of EEG or ECoG signals recorded during epileptic seizures.
The method allows to visualize the spatio-temporal evolution
of synchronization and coupling effects that are characteristic
for epileptic seizures. Similar to other methods proposed for
this purpose our method is based on a regression analysis.
However, a more general definition of the regression together
with an effective channel selection procedure allows to use the
method even for time series that are highly correlated, which
is commonly the case in EEG/ECoG recordings with large
numbers of electrodes. The method was experimentally tested
on ECoG recordings of epileptic seizures from patients with
temporal lobe epilepsies. A comparision with the results from
a independent visual inspection by clinical experts showed
an excellent agreement with the patterns obtained with the
proposed method.