Abstract: The field research was carried out at the Látókép AGTC KIT research area of the University of Debrecen in Eastern-Hungary, on the area of the aeolain loess of the Hajdúság. We examined the effects of the sowing time on the phytopathogenic characteristics and yield production by applying various fertilizer treatments on two different sunflower genotypes (NK Ferti, PR64H42) in 2012 and 2013. We applied three different sowing times (early, optimal, late) and two different treatment levels of fungicides (control = no fungicides applied, double fungicide protection).
During our investigations, the studied cropyears were of different sowing time optimum in terms of yield amount (2012: early, 2013: average). By Pearson’s correlation analysis, we have found that delaying the sowing time pronouncedly decreased the extent of infection in both crop years (Diaporthe: r=0.663**, r=0.681**, Sclerotinia: r=0.465**, r=0.622**). The fungicide treatment not only decreased the extent of infection, but had yield increasing effect too (2012: r=0.498**, 2013: r=0.603**). In 2012, delaying of the sowing time increased (r=0.600**), but in 2013, it decreased (r= 0.356*) the yield amount.
Abstract: Matrix metalloproteinase-3 (MMP3) is key member
of the MMP family, and is known to be present in coronary
atherosclerotic. Several studies have demonstrated that MMP-3
5A/6A polymorphism modify each transcriptional activity in allele
specific manner. We hypothesized that this polymorphism may play
a role as risk factor for development of coronary stenosis. The aim of
our study was to estimate MMP-3 (5A/6A) gene polymorphism on
interindividual variability in risk for coronary stenosis in an Iranian
population.DNA was extracted from white blood cells and genotypes
were obtained from coronary stenosis cases (n=95) and controls
(n=100) by PCR (polymerase chain reaction) and restriction
fragment length polymorphism techniques. Significant differences
between cases and controls were observed for MMP3 genotype
frequencies (X2=199.305, p< 0.001); the 6A allele was less
frequently seen in the control group, compared to the disease group
(85.79 vs. 78%, 6A/6A+5A/6A vs. 5A/5A, P≤0.001). These data
imply the involvement of -1612 5A/6A polymorphism in coronary
stenosis, and suggest that probably the 6A/6A MMP-3 genotype is a
genetic susceptibility factor for coronary stenosis.
Abstract: Selection of maize (Zea mays) hybrids with wide adaptability across diverse farming environments is important, prior to recommending them to achieve a high rate of hybrid adoption. Grain yield of 14 maize hybrids, tested in a randomized completeblock design with four replicates across 22 environments in Iran, was analyzed using site regression (SREG) stability model. The biplot technique facilitates a visual evaluation of superior genotypes, which is useful for cultivar recommendation and mega-environment identification. The objectives of this study were (i) identification of suitable hybrids with both high mean performance and high stability (ii) to determine mega-environments for maize production in Iran. Biplot analysis identifies two mega-environments in this study. The first mega-environments included KRM, KSH, MGN, DZF A, KRJ, DRB, DZF B, SHZ B, and KHM, where G10 hybrid was the best performing hybrid. The second mega-environment included ESF B, ESF A, and SHZ A, where G4 hybrid was the best hybrid. According to the ideal-hybrid biplot, G10 hybrid was better than all other hybrids, followed by the G1 and G3 hybrids. These hybrids were identified as best hybrids that have high grain yield and high yield stability. GGE biplot analysis provided a framework for identifying the target testing locations that discriminates genotypes that are high yielding and stable.
Abstract: Perennial ryegrass (Lolium perenne L.) plants are cultivated for lawn constitution and as forage plants. Considerable number of perennial ryegrass genotypes are present in the flora of our country and they present substantial was performed based on a Project supported bu TUBITAK (Project numver : 106O159) and perannial ryegrass genotypes from 8 provinces were collected during 2006. Seeds of perennial ryegrass were collected from 48 different locations. Populations of turfgrass seeds in flowerpots to be 20 and 1 cm deep greenhouse were sown in three replications at 07.07.2007.Then the growth of turfgrass seedlings in the greenhouse in pots showed sufficiently separated from the plants were planted in each population. Plants planted in the garden of the observation scale of 1-9 was evaluated by the quality, 1 = the weakest / worst, 6 = acceptable and 9 = superior or considered as an ideal. Essentially only recognized in assessing the quality of the color of grass, but the color, density, uniformity, texture (texture), illness or environmental stresses are evaluated as a combination reaction. Turfgrass quality 15.11.2007, 19.03.2008, 27.05.2008, 27.11.2008, 07.03.2009 and 02.06.2009 have been 6 times to be in order. Observations made regarding the quality of grass; 3 years according to seasonal environments turf quality genotypes belonging to 14 different populations were found to be 7.5 and above are reserved for future use in breeding works.The number of genotypes belonging to 41 populations in terms of turfgrass quality was determined as 7.9 of 3 year average seasonal. Argıthan between Doğanhisar (Konya) is located 38.09 latitude and 31.40 longitude, altitude 1158 m in the set that population numbered 41.
Abstract: Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.
Abstract: Processes of plant breeding, testing and licensing of new varieties, patent protection in seed production, relations in trade and protection of copyright are dependent on identification, differentiation and characterization of plant genotypes. Therefore, we focused our research on utilization of wheat storage proteins as genetic markers suitable not only for differentiation of individual genotypes, but also for identification and characterization of their considerable properties. We analyzed a collection of 102 genotypes of bread wheat (Triticum aestivum L.), 41 genotypes of spelt wheat (Triticum spelta L.), and 35 genotypes of durum wheat (Triticum durum Desf.), in this study. Our results show, that genotypes of bread wheat and durum wheat were homogenous and single line, but spelt wheat genotypes were heterogenous. We observed variability of HMW-GS composition according to environmental factors and level of breeding and predict technological quality on the basis of Glu-score calculation.
Abstract: The present study aimed to investigate whether
chlorophyll meter readings (SPAD) can be used as criterion of singleplant
selection in maize breeding. Experimentation was performed at
the ultra-low density of 0.74 plants/m2 in order the potential yield per
plant to be fully expressed. R-31 honeycomb experiments were
conducted in three different areas in Greece (Thessaloniki, Giannitsa
and Florina) using 30 inbred lines at well-watered and water-stressed
conditions during the 2012 growing season. The chlorophyll meter
readings had higher rates at dry conditions, except location of
Giannitsa where differences were not significant. Genotypes of
highest chlorophyll meter readings were consistent across areas,
emphasizing on the character’s stability. A positive correlation
between the chlorophyll meter readings and grain yield was
strengthening over time and culminated at the physiological maturity
stage. There was a clear sign that the chlorophyll meter readings has
the potential to be used for the selection of stress-adaptive genotypes
and may permit modern maize to be grown at wider range of
environments addressing the climate change scenarios.
Abstract: This research was conducted to determine responses
of chickpeas to drought in different periods (early period, late period,
no-irrigation, two times irrigation as control). The trial was made in
“Randomized Complete Block Design" with three replications on
2010 and 2011 years in Konya-Turkey. Genotypes were consisted
from 7 lines of ICARDA, 2 certified lines and 1 local population. The
results showed that; as means of years and genotypes, early period
stress showed highest (207.47 kg da-1) seed yield and it was followed
by control (202.33 kg da-1), late period (144.64 kg da-1) and normal
(106.93 kg da-1) stress applications. The genotypes were affected too
much by drought and, the lowest seed was taken from non-irrigated
plots. As the means of years and stress applications, the highest
(196.01 kg da-1) yield was taken from genotype 22255. The reason of
yield variation could be derived from different responses of
genotypes to drought.
Abstract: The subcellular organelles called oil bodies (OBs) are lipid-filled quasi-spherical droplets produced from the endoplasmic reticulum (ER) and then released into the cytoplasm during seed development. It is believed that an OB grows by coalescence with other OBs and that its stability depends on the composition of oleosins, major proteins inserted in the hemi membrane that covers OBs. In this study, we measured the OB-volume distribution from different genotypes of A. thaliana after 7, 8, 9, 10 and 11 days of seed development. In order to test the hypothesis of OBs dynamics, we developed a simple mathematical model using non-linear differential equations inspired from the theory of coagulation. The model describes the evolution of OB-volume distribution during the first steps of seed development by taking into consideration the production of OBs, the increase of triacylglycerol volume to be stored, and the growth by coalescence of OBs. Fitted parameters values show an increase in the OB production and coalescence rates in A. thaliana oleosin mutants compared to wild type.
Abstract: Water is the main component of biological processes.
Water management is important to obtain higher productivity. In this
study, some of the yield components were investigated together with
different drought levels. Four chickpea genotypes (CDC Frontier,
CDC Luna, Sawyer and Sierra) were grown in pots with 3 different
irrigation levels (a dose of 17.5 ml, 35 ml and 70 ml for each pot per
day) after three weeks from sowing. In the research, flowering, pod
set, pod per plant, fertile pod, double seed/pod, stem diameter, plant
weight, seed per plant, 1000 seed weight, seed diameter, vegetation
length and weekly plant height were measured. Consequently,
significant differences were observed on all the investigated
characteristics owing to genotypes (except double seed/pod and stem
diameter), water levels (except first pod, seed weight and height on
3rd week) and genotype x water level interaction (except first pod,
double seed/pod, seed weight and height).
Abstract: Type 2 diabetes mellitus (T2DM) is a complex
metabolic disorder that characterized by the presence of high glucose
in blood that cause from insulin resistance and insufficiency due to
deterioration β-cell Langerhans functions. T2DM is commonly
caused by the combination of inherited genetic variations as well as
our own lifestyle. Metallothionein (MT) is a known cysteine-rich
protein responsible in helping zinc homeostasis which is important in
insulin signaling and secretion as well as protection our body from
reactive oxygen species (ROS). MT scavenged ROS and free
radicals in our body happen to be one of the reasons of T2DM and its
complications. The objective of this study was to investigate the
association of MT1A and MT2A polymorphisms between T2DM and
control subjects among Malay populations. This study involved 150
T2DM and 120 Healthy individuals of Malay ethnic with mixed
genders. The genomic DNA was extracted from buccal cells and
amplified for MT1A and MT2A loci; the 347bp and 238bp banding
patterns were respectively produced by mean of the Polymerase
Chain Reaction (PCR). The PCR products were digested with Mlucl
and Tsp451 restriction enzymes respectively and producing
fragments lengths of (158/189/347bp) and (103/135/238bp)
respectively. The ANOVA test was conducted and it shown that there
was a significant difference between diabetic and control subjects for
age, BMI, WHR, SBP, FPG, HBA1C, LDL, TG, TC and family
history with (P0.05). The genotype
frequency for AA, AG and GG of MT1A polymorphisms was 72.7%,
22.7% and 4.7% in cases and 15%, 55% and 30% in control
respectively. As for MT2A, genotype frequency of GG, GC and CC
was 42.7%, 27.3% and 30% in case and 5%, 40% and 55% for
control respectively. Both polymorphisms show significant difference
between two investigated groups with (P=0.000). The Post hoc test
was conducted and shows a significant difference between the
genotypes within each polymorphism (P=0. 000). The MT1A and
MT2A polymorphisms were believed to be the reliable molecular
markers to distinguish the T2DM subjects from healthy individuals in
Malay populations.
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.
Abstract: This study carried out in order to investigate the
effects of salinity on carbon isotope discrimination (Δ) of shoots and
roots of four sugar beet cultivars (cv) including Madison (British
origin) and three Iranian culivars (7233-P12, 7233-P21 and 7233-P29).
Plants were grown in sand culture medium in greenhouse conditions.
Plants irrigated with saline water (tap water as control, 50 mM, 150
mM, 250 mM and 350 mM of NaCl + CaCl2 in 5 to 1 molar ratio)
from 4 leaves stage for 16 weeks. Carbon isotope discrimination
significantly decreased with increasing salinity. Significant
differences of Δ between shoot and root were observed in all cvs and
all levels of salinity. Madison cv showed lower Δ in shoot and root
than other three cvs at all levels of salinity expect control, but cv
7233-P29 had significantly higher Δ values at saline conditions of 150
mM and above. Therefore, Δ might be applicable, as a useful tool, for
study of salinity tolerance of sugar beet genotypes.
Abstract: The influence of humidity and low temperature on the α- amylase activity and isoenzyme composition of grains of different wheat varieties have been studied. The identified samples of varieties have significant difference in the level of enzyme induction under the impact of high humidity and low temperature. It is proposed to use this methodological approach for testing genotypes and wheat breeding lines for resistance to pre-harvest sprouting (PHS).
Abstract: A lot of recent research have spoken on the relation
between the increase of the homocysteinemia and some kinds of
cancer . For that, our study was based on the research of a possible
relation between the increase of the concentration of this amino-acid
in the plasma and the appearance of the disease of the Acute
Lymphoblastic Leukaemia in a part of Algerian children with Berber
origin in the East of Algeria . The study has done on 47 ill persons
with an average age of (09±06 ) years , with whom the disease has
diagnosed by blood and marrow examination in the hospital of blood
diseases in the CHU of Batna, and on 194 healthy witnesses of the
same age. The two groups were benefited by a dosage of the
concentration of the homocysteine vitamin B9 ,vitamin B12 , and
also of the study of special polymorphisms of indispensable enzymes
in the metabolism of this acid , and that by the use of the method (
Light cycler ) Real time PCR , on the following enzymes : MS (
C2756G ), MSR ( A66G ) ,MTHFR1 ( C677T ) and MTHFR2
(A1298C). The obtained results have revealed that the rate of the
homozygote muted genotype is the less frequent in the two groups ,
and that exist at list one genotype of each enzyme in the ill group and
in which the percentage exceed with remarkable way the same
genotype in the healthy group and we notice specially the muted
genotype GG of -the methionine synthetase-and the form TT of the
enzyme – methyline tetra hydrofolate reductase – We notice the
existence of considerable number of genotypes in the ill group lied
with characteristic increase of this Amino-acid ,and that for the
reduction of the biologic activity of these enzymes which become
inefficient in the transfer of the homocysteine into the methionine
and cause the diminution of the biologic activity of these enzymes
and with consequence the reduction of the percentage of methylic
radicals in the DNA of studied genes and that lead to the increase of
the activity and the capacity of transcription , and it-s so probably
that this last one is one of the factors of this disease especially if we
know that the specific check-up of vitamins is normal and similar in
the two groups , which ovoid the hypothesis of the reduction of
vitamins . We notice also that the heterozygote genotype is the less in
the sick category except the MTHFR2. Wild genotype is more
frequent in the witness group except MSR. Even these results are
partials; they open a new way in the genetic diagnosis of this
malicious disease which allow a precocious diagnosis and the use of
an effective and appropriated treatment in the same time.
Abstract: Post-anthesis drought stress is the most important
problem affecting wheat production in dryland fields, specially in
Mediterranean regions. The main objective of this research was to
evaluate drought tolerance indices in dryland wheat genotypes under
post-anthesis drought stress. The research was including two different
experiments. In each experiment, twenty dryland bread wheat
genotypes were sown in a randomized complete blocks design
(RCBD) with three replications. One of experiments belonged to
rain-fed conditions (post-anthesis drought stress) and other
experiment was under non-stress conditions (with supplemental
irrigation). Different drought tolerance indices include Stress
Tolerance (Tol), Mean Productivity (MP), Geometric Mean
Productivity (GMP), Stress Susceptibility Index (SSI), Stress
Tolerance Index (STI), Harmonic Mean (HAM), Yield Index (YI)
and Yield Stability Index (YSI) were evaluate based on grain yield
under rain-fed (Ys) and supplemental irrigation (Yp) environments.
G10 and G12 were the most tolerant genotypes based on TOL and
SSI. But, based on MP, GMP, STI, HAM and YI indices, G1 and G2
were selected. STI, GMP and MP indices had high correlation with
grain yield under rain-fed and supplementary irrigation conditions
and were recognized as appropriate indices to identify genotypes with
high grain yield and low sensitivity to drought stress environments.
Abstract: The selection of parents and breeding strategies for
the successful maize hybrid production will be facilitated by
heterotic groupings of parental lines and determination of combining
abilities of them. Fourteen maize inbred lines, used in maize breeding
programs in Iran, were crossed in a diallel mating design. The 91 F1
hybrids and the 14 parental lines were studied during two years at
four locations of Iran for investigation of combining ability of
gentypes for grain yield and to determine heterotic patterns among
germplasm sources, using both, the Griffing-s method and the biplot
approach for diallel analysis. The graphical representation offered by
biplot analysis allowed a rapid and effective overview of general
combining ability (GCA) and specific combining ability (SCA)
effects of the inbred lines, their performance in crosses, as well as
grouping patterns of similar genotypes. GCA and SCA effects were
significant for grain yield (GY). Based on significant positive GCA
effects, the lines derived from LSC could be used as parent in crosses
to increase GY. The maximum best- parent heterosis values and
highest SCA effects resulted from crosses B73 × MO17 and A679 ×
MO17 for GY. The best heterotic patterns were LSC × RYD, which
would be potentially useful in maize breeding programs to obtain
high-yielding hybrids in the same climate of Iran.
Abstract: The common bean is the most important grain legume for direct human consumption in the world and BCMV is one of the world's most serious bean diseases that can reduce yield and quality of harvested product. To determine the best tolerance index to BCMV and recognize tolerant genotypes, 2 experiments were conducted in field conditions. Twenty five common bean genotypes were sown in 2 separate RCB design with 3 replications under contamination and non-contamination conditions. On the basis of the results of indices correlations GMP, MP and HARM were determined as the most suitable tolerance indices. The results of principle components analysis indicated 2 first components totally explained 98.52% of variations among data. The first and second components were named potential yield and stress susceptible respectively. Based on the results of BCMV tolerance indices assessment and biplot analysis WA8563-4, WA8563-2 and Cardinal were the genotypes that exhibited potential seed yield under contamination and noncontamination conditions.