Abstract: An HPLC-UV analytical method was developed to
determine ethylenediaminetetraacetic acid (EDTA) in dairy
wastewater and surface water. The optimizing separation was achieved
by reversed–phase ion-pair liquid chromatography on a C18 column
using methanol as mobile phase solvent, tetrabutylammonium bromide
as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate
of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca,
Mg or NO3
- was detected. Method performance was evaluated in terms
of linearity, repeatability and reproducibility. The method detection
limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72 ~
261 μg L-1 at a large dairy site. A change of EDTA concentration was
observed downstream of the dairy effluent discharge, but this was well
under the predicted no effect concentration for aquatic ecosystem.
Abstract: Powerline Communications –PLC– as an alternative
method for broadband networking, has the advantage of transmitting
over channels already used for electrical distribution or even
transmission. But these channels have been not designed with usual
wired channels requirements for broadband applications such as
stable impedance or known attenuation, and the network have to
reject noises caused by electrical appliances that share the same
channel. Noise control standards are difficult to complain or simply
do not exist on Latin-American environments. This paper analyzes
PLC throughput for home connectivity by probing noisy channel
scenarios in a PLC network and the statistical results are shown.
Abstract: The present investigation was aimed to develop methodology for the standardization of Marichyadi Vati and its raw materials. Standardization was carried using systematic Pharmacognostical and physicochemical parameters as per WHO guidelines. The detailed standardization of Marichyadi Vati, it is concluded that there are no major differences prevailed in the quality of marketed products and laboratory samples of Marichyadi Vati. However, market samples showed slightly better amount of Piperine than the laboratory sample by both methods. This is the first attempt to generate complete set of standards required for the Marichyadi Vati.
Abstract: Pummelo is known to be the largest of all citrus fruits, with expected ratio of 2:1 (w/v) of producing juice, is an attractive opportunity for Malaysia to expand pummelo-s influence and marketability over the international market of juices. The purpose of this study is to identify and quantify the phenolic compounds in two Malaysian varieties of pummelo fruit juice: Ledang (PO55) and Tambun (PO52). Identifications of polyphenols composition were done using High Performance Liquid Chromatography Diode Array Detection (HPLC-DAD). The phenolic compounds that were found in both varieties were hydroxycinnamic acids and flavonones. This study proved that Tambun variety has the highest antioxidant and phenolic compounds in comparison to Ledang variety. However, considerations have to be made to suit consumer-s taste bud and the amount of enzyme needed to clarify the juice for its marketability.
Abstract: Mycotoxin (aflatoxins) contamination of peanuts is a
great concern for human health. A total of 72 samples of unripe,
roasted, and salty peanuts were collected randomly from Pothohar
plateau of Pakistan for the assessment of aflatoxin. Samples were
dried, ground and extracted by acetonitrile (84%). The filtered
extracts were cleaned up by MycoSep-226 and analyzed by high
performance liquid chromatography with flourescence detector.
Quantification limit of Aflatoxin was 1 μg/kg and 70% Recovery was
observed in spiked samples in the range 1–10 μg/kg. The screening
of mycotoxins indicated that aflatoxins were present in most of the
samples being detected in 82%, in concentrations from 14.25 μg/kg
to 98.80 μg/kg. Optimal conditions for mycotoxin production and
fungal growth are frequently found in the crop fields as well as in
store houses. Human exposure of such toxin can be controlled by
pointed out such awareness and implemented the regulations.
Abstract: The agricultural organic farming is different from
conventional farming in a way that is aimed at providing a balanced
and constructive action in agricultural systems. With the increase in
intensive agriculture, undesirable changes were being observed in
ecosystems with irreparable damage being caused to the natural
equilibrium. This is the reason for the increasing interest in organic
farming as an environment friendly agricultural production method.
In the present work three red fruits produced in organic farming were
analyzed, namely raspberry, gooseberry and blueberry. The samples
were harvested in a local farm when at plain maturation. The results
obtained allowed to conclude that the blueberry contained higher
amounts of phenolic compounds, total tannins and total anthocyanins
than raspberry and gooseberry. Furthermore, the HPLC analysis
allowed to identify monomeric anthocyanins and phenolic acids in
the three fruits studied.
Abstract: This paper uses p-tolerance with the lowest posterior
loss, quadratic loss function, average length criteria, average
coverage criteria, and worst outcome criterion for computing of
sample size to estimate proportion in Binomial probability function
with Beta prior distribution. The proposed methodology is examined,
and its effectiveness is shown.
Abstract: Programmable logic controllers are the main controllers in the today's industries; they are used for several applications in industrial control systems and there are lots of examples exist from the PLC applications in industries especially in big companies and plants such as refineries, power plants, petrochemical companies, steel companies, and food and production companies. In the PLCs there are some functions in the function library in software that can be used in PLC programs as basic program elements. The aim of this project are introducing and implementing a new function block of a neural network to the function library of PLC. This block can be applied for some control applications or nonlinear functions calculations after it has been trained for these applications. The implemented neural network is a Perceptron neural network with three layers, three input nodes and one output node. The block can be used in manual or automatic mode. In this paper the structure of the implemented function block, the parameters and the training method of the network are presented by considering the especial method of PLC programming and its complexities. Finally the application of the new block is compared with a classic simulated block and the results are presented.
Abstract: The extract of milk thistle contains a mix of flavonolignans termed silymarine.. In order to analysis influence of growth regulators, genotype, explant and subculture on the accumulation of flavonolignans, a study was carried out by using two genotype (Budakalszi and Noor abad moghan cultivars), cotyledon and hypocotyle explants, solid media of MS supplemented by different combinations of two growth regulators; Kinetin (0.1, 1 mg/l) and 2,4-D (1, 2 mg/l). Seeds of the plant were germinated in MS media whitout growth regulators in growth chamber at 26°C and darkness condition. In order to callus induction, the culture media was supplemented whit different concentrations of 2,4-D and kinetin. Calli obtained from explants were sub-cultured four times into the fresh media of the first experiment. flavonoides was extracted from calli in four subcultures. The flavonoid components were determined by high- performance liquid choromatography (HPLC) and separated into Taxifolin, Silydianin+Silychristin, Silybin A+B and Isosilybin A+B. Results showed that with increasing callus age, increased accumulation of silybin A+B, but reduced Isosilybin A+B content. Highest accumulation of Taxifolin was observed at first calli. Calli produced from cotyledon explant of Budakalszi cultivar were superior for Silybin A+B, where calli from hypocotyl explant produced higher amount of Taxifolin and Silydianin+Silychristin. The best cultivar for Silymarin production in this study was Budakalszi cultivar. High amount of SBN A+B and TXF were obtained from hypocotil explant.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: A laboratory set-up was designed to survey the
effectiveness of UV/O3 advanced oxidation process (AOP) for the
removal of Carbaryl from polluted water in batch reactor. The study
was carried out by UV/O3 process for water samples containing 1 to
20 mg/L of Carbaryl in distilled water. Also the range of drinking
water resources adjusted in synthetic water and effects of contact
time, pH and Carbaryl concentration were studied. The residual
pesticide concentration was determined by applying high
performance liquid chromatography (HPLC). The results indicated
that increasing of retention time and pH, enhances pesticide removal
efficiency. The removal efficiency has been affected by pesticide
initial concentration. Samples with low pesticide concentration
showed a remarkable removal efficiency compared to the samples
with high pesticide concentration. AOP method showed the removal
efficiencies of 80% to 100%. Although process showed high
performance for removal of pesticide from water samples, this
process has different disadvantages including complication,
intolerability, difficulty of maintenance and equipmental and
structural requirements.
Abstract: This paper presents a reliability-based approach to select appropriate wind turbine types for a wind farm considering site-specific wind speed patterns. An actual wind farm in the northern region of Iran with the wind speed registration of one year is studied in this paper. An analytic approach based on total probability theorem is utilized in this paper to model the probabilistic behavior of both turbines- availability and wind speed. Well-known probabilistic reliability indices such as loss of load expectation (LOLE), expected energy not supplied (EENS) and incremental peak load carrying capability (IPLCC) for wind power integration in the Roy Billinton Test System (RBTS) are examined. The most appropriate turbine type achieving the highest reliability level is chosen for the studied wind farm.
Abstract: We present a white LED-based optical wireless
communication systems for indoor ubiquitous sensor networks. Each
sensor node could access to the server through the PLC (Power Line
Communication)-Ethernet interface. The proposed system offers a
full-duplex wireless link by using different wavelengths to reduce the
inter-symbol interference between uplink and downlink. Through the
1-to-n optical wireless sensor network and PLC modem, the mobile
terminals send a temperature data to server. The data transmission
speed and distance are 115.2kbps and about 60cm, respectively.
Abstract: The system is made with main distributed components:
First Level: Industrial Computers placed in Control Room (monitors thermal and electrical processes based on the data provided by the second level); Second Level: PLCs which collects data from process and transmits information on the first level; also takes commands from this level which are further, passed to execution elements from third
level; Third Level: field elements consisting in 3 categories: data collecting elements; data transfer elements from the third level to the second; execution elements which take commands from the second
level PLCs and executes them after which transmits the confirmation of execution to them. The purpose of the automatic functioning is the optimization of the co-generative electrical energy commissioning in the national
energy system and the commissioning of thermal energy to the consumers.
The integrated system treats the functioning of all the equipments and devices as a whole: Gas Turbine Units (GTU); MT 20kV Medium Voltage Station (MVS); 0,4 kV Low Voltage Station (LVS); Main Hot Water Boilers (MHW); Auxiliary Hot Water Boilers (AHW); Gas Compressor Unit (GCU); Thermal Agent Circulation
Pumping Unit (TPU); Water Treating Station (WTS).
Abstract: Lutein is a dietary oxycarotenoid which is found
to reduce the risks of Age-related Macular Degeneration
(AMD). Supercritical fluid extraction of lutein esters from
marigold petals was carried out and was found to be much
effective than conventional solvent extraction. The
saponification of pre-concentrated lutein esters to produce free
lutein was studied which showed a composition of about 88%
total carotenoids (UV-VIS spectrophotometry) and 90.7%
lutein (HPLC). The lipase catalyzed hydrolysis of lutein esters
in conventional medium was investigated. The optimal
temperature, pH, enzyme concentration and water activity
were found to be 50°C, 7, 15% and 0.33 respectively and the
activity loss of lipase was about 25% after 8 times re-use in at
50°C for 12 days. However, the lipase catalyzed hydrolysis of
lutein esters in conventional media resulted in poor
conversions (16.4%).
Abstract: The changes in quality properties and nutritional
components in two fermented mugworts (Artemisia capillaries
Thumberg, Artemisiaeasiaticae Nakai) were characterized followed
by the rapid pattern analysis of volatile flavor compounds by Electric
Nose based on SAW(Surface Acoustic Wave) sensor in GC system.
There were remarkable decreases in the pH and small changes in the
total soluble solids after fermentation. The L (lightness) and b
(yellowness) values in Hunter's color system were shown to be
decreased, whilst the a (redness) value was increased by fermentation.
The HPLC analysis demonstrated that total amino acids were
increased in quantity and the essential amino acids were contained
higher in A. asiaticaeNakai than in A. capillaries Thumberg. While
the total polyphenol contents were not affected by fermentation, the
total sugar contents were dramatically decreased. Scopoletinwere
highly abundant in A. capillarisThumberg, however, it was not
detected in A. asiaticaeNakai. Volatile flavor compounds by Electric
Nose showed that the intensity of several peaks were increased much
and seven additional flavor peaks were newly produced after
fermentation. The flavor differences of two mugworts were clearly
distinguished from the image patterns of VaporPrintTM which indicate
that the fermentation enables the two mugworts to have subtle flavor
differences.
Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: The consumption of lactose in acid cheese whey
anaerobic fermentation process under fed-batch conditions was
studied. During fermentation for 100 hours the biogas production
(CO2 and CH4) was analyzed online. Among the standard analyses
FT-IR spectroscopy was used to follow the consumption of lactose by
bacteria. The absorption bands at 990, 894 and 787 cm-1 in the 2nd
derivative spectra were shown to be characteristic for lactose and
were used to follow the lactose conversion. It was shown that acid
cheese whey lactose was converted by bacteria in first 7 hours. In the
spectra of 17, 18 and 95 hour fermentation samples lactose was not
identified and these results correlated with the HPLC data.
Abstract: This paper presents an application of power line
carrier (PLC) for electrical power telemetering. This system has a
special capability of transmitting the measured values to a centralized
computer via power lines. The PLC modem as a passive high-pass
filter is designed for transmitting and receiving information. Its
function is to send the information carrier together with transmitted
data by superimposing it on the 50 Hz power frequency signal. A
microcontroller is employed to function as the main processing of the
modem. It is programmed for PLC control and interfacing with other
devices. Each power meter, connected via a PLC modem, is assigned
with a unique identification number (address) for distinguishing each
device from one another.
Abstract: The paper compares different channel models used for
modeling Broadband Power-Line Communication (BPLC) system.
The models compared are Zimmermann and Dostert, Philipps,
Anatory et al and Anatory et al generalized Transmission Line (TL)
model. The validity of each model was compared in time domain
with ATP-EMTP software which uses transmission line approach. It
is found that for a power-line network with minimum number of
branches all the models give similar signal/pulse time responses
compared with ATP-EMTP software; however, Zimmermann and
Dostert model indicates the same amplitude but different time delay.
It is observed that when the numbers of branches are increased only
generalized TL theory approach results are comparable with ATPEMTP
results. Also the Multi-Carrier Spread Spectrum (MC-SS)
system was applied to check the implication of such behavior on the
modulation schemes. It is observed that using Philipps on the
underground cable can predict the performance up to 25dB better
than other channel models which can misread the actual performance
of the system. Also modified Zimmermann and Dostert under
multipath can predict a better performance of about 5dB better than
the actual predicted by Generalized TL theory. It is therefore
suggested for a realistic BPLC system design and analyses the model
based on generalized TL theory be used.