Abstract: We studied the evolution of elliptic heavy SF6
gas cylinder surrounded by air when accelerated by a planar
Mach 1.25 shock. A multiple dynamics imaging technology has
been used to obtain one image of the experimental initial
conditions and five images of the time evolution of elliptic
cylinder. We compared the width and height of the circular and
two kinds of elliptic gas cylinders, and analyzed the vortex
strength of the elliptic ones. Simulations are in very good
agreement with the experiments, but due to the different initial
gas cylinder shapes, a certain difference of the initial density
peak and distribution exists between the circular and elliptic
gas cylinders, and the latter initial state is more sensitive and
more inenarrable.
Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: Hydrogen that used as fuel in fuel cell vehicles can be
produced from renewable sources such as wind, solar, and hydro
technologies. PV-electrolyzer is one of the promising methods to
produce hydrogen with zero pollution emission. Hydrogen
production from a PV-electrolyzer system depends on the efficiency
of the electrolyzer and photovoltaic array, and sun irradiance at that
site. In this study, the amount of hydrogen is obtained using
mathematical equations for difference driving distance and sun peak
hours. The results show that the minimum of 99 PV modules are used
to generate 1.75 kgH2 per day for two vehicles.
Abstract: Internet today has a huge impact on all aspects of life,
and also in the area of the broader context of democracy, politics and
politicians. If democracy is freedom of choice, there are a number of
conditions that can ensure in practice the freedom to be achieved and
realized. These preconditions must be achieved regardless of the
manner of voting. The key contribution of ICT to achieve freedom of
choice is that technology enables the correlation of the citizens and
elected representatives on the better way than it was possible without
the Internet. In this sense, we can say that the Internet and ICT are
changing significantly, and potentially improving the environment in
which democratic processes are taking place. This paper aims to
describe trends in use of ICT in democratic processes, and analyzes
the challenges for implementation of e-Democracy in Montenegro