Abstract: The primary objective of this work was to study the
effect of resin chemistry, pH and molarity of binding and elution
buffer on aggregate removal using Cation Exchange Chromatography
and find the optimum conditions which can give efficient aggregate
removal with minimum loss of yield. Four different resins were used
for carrying out the experiments: Fractogel EMD SO3
-(S), Fractogel EMD COO-(M), Capto SP ImpRes and S Ceramic HyperD. Runs
were carried out on the AKTA Avant system. Design of Experiments
(DOE) was used for analysis using the JMP software. The
dependence of the yield obtained using different resins on the
operating conditions was studied. Success has been achieved in
obtaining yield greater than 90% using Capto SP ImpRes and
Fractogel EMD COO-(M) resins. It has also been found that a change
in the operating conditions generally has different effects on the
yields obtained using different resins.
Abstract: Mustard leaves are rich in folates, vitamin A, K and
B-complex. Mustard greens are low in calories and fats and rich in
dietary fiber. They are rich in potassium, manganese, iron, copper,
calcium, magnesium and low in sodium. It is very rich in antioxidants
and Phytonutrients. For the optimization of process variables
(moisture content and mustard leave powder), the experiments were
conducted according to central composite Face Centered Composite
design of RSM. The mustard leaves powder was replaced with
composite flour (a combination of rice, chickpea and corn in the ratio
of 70:15:15). The extrudate was extruded in a twin screw extruder at
a barrel temperature of 120°C. The independent variables were
mustard leaves powder (2-10 %) and moisture content (12-20 %).
Responses analyzed were bulk density, water solubility index, water
absorption index, lateral expansion, antioxidant activity, total
phenolic content, and overall acceptability. The optimum conditions
obtained were 7.19 g mustard leaves powder in 100g premix having
16.8% moisture content (w.b).
Abstract: In this research paper were investigated the main
regularities of a radical bromination reaction of decalin. There had
been studied the temperature effect, durations of reaction, frequency
rate of process, a ratio of initial components, type and number of the
initiator on decalin bromination degree.
There were specified optimum conditions of synthesis of a
perbromodecalin by the method of a decalin bromination. There are
developed the technological flowchart of receiving a
perbromodecalin and the mass balance of process on the first and the
subsequent loadings of components.
The results of research of antibacterial and antifungal activity of
synthesized bromoderivatives have been represented.
Abstract: Bacterial strains capable of degradation of malathion
from the domestic sewage were isolated by an enrichment culture
technique. Three bacterial strains were screened and identified as
Acinetobacter baumannii (AFA), Pseudomonas aeruginosa (PS1),
and Pseudomonas mendocina (PS2) based on morphological,
biochemical identification and 16S rRNA sequence analysis.
Acinetobacter baumannii AFA was the most efficient malathion
degrading bacterium, so used for further biodegradation study. AFA
was able to grow in mineral salt medium (MSM) supplemented with
malathion (100 mg/l) as a sole carbon source, and within 14 days,
84% of the initial dose was degraded by the isolate measured by high
performance liquid chromatography. Strain AFA could also degrade
other organophosphorus compounds including diazinon, chlorpyrifos
and fenitrothion. The effect of different culture conditions on the
degradation of malathion like inoculum density, other carbon or
nitrogen sources, temperature and shaking were examined.
Degradation of malathion and bacterial cell growth were accelerated
when culture media were supplemented with yeast extract, glucose
and citrate. The optimum conditions for malathion degradation by
strain AFA were; an inoculum density of 1.5x 10^12CFU/ml at 30°C
with shaking. A specific polymerase chain reaction primers were
designed manually using multiple sequence alignment of the
corresponding carboxylesterase enzymes of Acinetobacter species.
Sequencing result of amplified PCR product and phylogenetic
analysis showed low degree of homology with the other
carboxylesterase enzymes of Acinetobacter strains, so we suggested
that this enzyme is a novel esterase enzyme. Isolated bacterial strains
may have potential role for use in bioremediation of malathion
contaminated.
Abstract: Multiwall carbon nanotubes, prepared by chemical
vapor deposition, have an average diameter of 60-100 nm as shown
by High Resolution Transmittance Electron Microscope, HR-TEM.
The Multiwall carbon nanotubes (MWCNTs) were further
characterized using X-ray Diffraction and Raman Spectroscopy.
Mercury uptake capacity of MWCNTs was studied using batch
adsorption method at different concentration ranges up to 150 ppm.
Mercury concentration (before and after the treatment) was measured
using cold vapor atomic absorption spectroscopy. The effect of time,
concentration, pH and adsorbent dose were studied. MWCNT were
found to perform complete absorption in the sub-ppm concentrations
(parts per billion levels) while for high concentrations, the adsorption
efficiency was 92% at the optimum conditions; 0.1 g of the adsorbent
at 150 ppm mercury (II) solution. The adsorption of mercury on
MWCNTs was found to follow the Freundlich adsorption isotherm
and the pseudo-second order kinetic model.
Abstract: Ferulic acid has widespread industrial potential by virtue of its antioxidant properties. However, it is partially soluble in aqueous media, limiting their usefulness in oil-based processes in food, cosmetic, pharmaceutical, and material industry. Therefore, modification of ferulic acid should be made by producing of more lipophilic derivatives. In this study, a preliminary investigation of lipase-catalyzed trans-esterification reaction of ethyl ferulate and olive oil was investigated. The reaction was catalyzed by immobilized lipase from Candida antarctica (Novozym 435), to produce ferulate ester, a sunscreen agent. A statistical approach of Response surface methodology (RSM) was used to evaluate the interactive effects of reaction temperature (40-80°C), reaction time (4-12 hours), and amount of enzyme (0.1-0.5 g). The optimum conditions derived via RSM were reaction temperature 60°C, reaction time 2.34 hours, and amount of enzyme 0.3 g. The actual experimental yield was 59.6% ferulate ester under optimum condition, which compared well to the maximum predicted value of 58.0%.
Abstract: The aluminum impregnated catalysts of Al-alumina (Al-Al2O3), Al-montmorillonite (Al-Mmn) and Al-activated charcoal (Al-AC) of various percent loadings were prepared by wet impregnation method and characterized by SEM, XRD and N2 adsorption/desorption (BET). The catalytic properties were investigated in the degradation of waste polystyrene (WPS). The results of catalytic degradation of Al metal, 20% Al-Al2O3, 5% Al-Mmn and 20% Al-AC were compared with each other for optimum conditions. Among the catalyst used 20% Al-Al2O3 was found the most effective catalyst. The BET surface area of 20% Al-Al2O3 determined was 70.2 m2/g. The SEM data revealed the catalyst with porous structure throughout the frame work with small nanosized crystallites. The yield of liquid products with 20% Al-Al2O3 (91.53 ± 2.27 wt%) was the same as compared to Al metal (91.20 ± 0.35 wt%) but the selectivity of hydrocarbons and yield of styrene monomer (56.32 wt%) was higher with 20% Al-Al2O3 catalyst.
Abstract: Rapid industrialization has led to increased disposal of heavy metals into the environment. Activated carbon adsorption has proven to be an effective process for the removal of trace metal contaminants from aqueous media. This paper was investigated chromium adsorption efficiency by commercial activated carbon. The sorption studied as a function of activated carbon particle size, dose of activated carbon and initial pH of solution. Adsorption tests for the effects of these factors were designed with Taguchi approach. According to the Taguchi parameter design methodology, L9 orthogonal array was used. Analysis of experimental results showed that, the most influential factor was initial pH of solution. The optimum conditions for chromium adsorption by activated carbons were found to be as follows: initial feed pH 6, adsorbent particle size 0.412 mm and activated carbon dose 6 g/l. Under these conditions, nearly %100 of chromium ions was adsorbed by activated carbon after 2 hours.
Abstract: In this research, the optimum conditions for the synthesis of activated carbon from biodiesel wastes such as palm shells (PS) and Jatropha curcas fruit shells (JS) by chemical activation method using potassium hydroxide (KOH) as an activating agent under nitrogen atmosphere were investigated. The effects of soaking in hydrofluoric acid (HF), impregnation ratio, activation temperature and activation time on adsorption capacity of methylene blue (MB) and iodine (I2) solution were examined. The results showed that HF-treated activated carbons exhibited higher adsorption capacities by eliminating ash residues, which might fill up the pores. In addition, the adsorption capacities of methylene blue and iodine solution were also significantly influenced by the types of raw materials, the activation temperature and the activation time. The highest adsorption capacity of methylene blue 257.07mg/g and iodine 847.58mg/g were obtained from Jatropha curcas wastes.
Abstract: In this work, sorption of nickel from aqueous solution on hypnea valentiae, red macro algae, was investigated. Batch experiments have been carried out to find the effect of various parameters such as pH, temperature, sorbent dosage, metal concentration and contact time on the sorption of nickel using hypnea valentiae. Response surface methodology (RSM) is employed to optimize the process parameters. Based on the central composite design, quadratic model was developed to correlate the process variables to the response. The most influential factor on each experimental design response was identified from the analysis of variance (ANOVA). The optimum conditions for the sorption of nickel were found to be: pH – 5.1, temperature – 36.8oC, sorbent dosage – 5.1 g/L, metal concentration – 100 mg/L and contact time – 30 min. At these optimized conditions the maximum removal of nickel was found to be 91.97%. A coefficient of determination R2 value 0.9548 shows the fitness of response surface methodology in this work.
Abstract: The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 min before being fed to the ASBR unit. The ASBR system was operated at different COD loading rates at a thermophillic temperature (55 °C), and controlled pH of 5.5. When the system was operated under optimum conditions (providing maximum hydrogen production performance) at a feed COD of 60 000 mg/l, and a COD loading rate of 68 kg/m3 d, the produced gas contained 43 % H2 content in the produced gas. Moreover, the hydrogen yield and the specific hydrogen production rate (SHPR) were 130 ml H2/g COD removed and 2100 ml H2/l d, respectively.
Abstract: Coal tar is a liquid by-product of the process of coal
gasification and carbonation. This liquid oil mixture contains various
kinds of useful compounds such as phenol, o-cresol, and p-cresol.
These compounds are widely used as raw material for insecticides,
dyes, medicines, perfumes, coloring matters, and many others.
This research needed to be done that given the optimum conditions
for the separation of phenol, o-cresol, and p-cresol from the coal tar
by solvent extraction process. The aim of the present work was to
study the effect of two kinds of aqueous were used as solvents:
methanol and acetone solutions, the effect of temperature (298, 306,
and 313K) and mixing (30, 35, and 40rpm) for the separation of
phenol, o-cresol, and p-cresol from coal tar by solvent extraction.
Results indicated that phenol, o-cresol, and p-cresol in coal tar
were selectivity extracted into the solvent phase and these
components could be separated by solvent extraction. The aqueous
solution of methanol, mass ratio of solvent to feed, Eo/Ro=1,
extraction temperature 306K and mixing 35 rpm were the most
efficient for extraction of phenol, o-cresol, and p-cresol from coal tar.
Abstract: In this study, lipase production has been investigated
using submerge fermentation by Aspergillus niger in Kilka fish oil as
main substrate. The Taguchi method with an L9 orthogonal array
design was used to investigate the effect of parameters and their
levels on lipase productivity. The optimum conditions for Kilka fish
oil concentration, incubation temperature and pH were obtained 3
gr./ml 35°C and 7, respectively. The amount of lipase activity in
optimum condition was obtained 4.59IU/ml. By comparing this
amount with the amount of productivity in the olive oil medium
based on the cost of each medium, it was that using Kilka fish oil is
84% economical. Therefore Kilka fish oil can be used as an
economical and suitable substrate in the lipase production and
industrial usages.
Abstract: In this research a mathematical model for direct
oxidization of hydrogen sulfide into elemental sulfur in a fluidized
bed reactor with external circulation was developed. As the catalyst
is deactivated in the fluidized bed, it might be placed in a reduction
tank in order to remove sulfur through heating above its dew point.
The reactor model demonstrated via MATLAB software. It was
shown that variations of H2S conversion as well as; products formed
were reasonable in comparison with corresponding results of a fixed
bed reactor. Through analyzing results of this model, it became
possible to propose the main optimized operating conditions for the
process considered. These conditions included; the temperature range
of 100-130ºC and utilizing the catalyst as much as possible providing
the highest bed density respect to dimensions of bed, economical
aspects that the bed ever remained in fluidized mode. A high active
and stable catalyst under the optimum conditions exhibited 100%
conversion in a fluidized bed reactor.
Abstract: In this research, a systematic investigation was carried out to determine the optimum conditions of HDS reactor. Moreover, a suitable model was developed for a rigorous RTO (real time optimization) loop of HDS (Hydro desulfurization) process. A systematic experimental series was designed based on CCD (Central Composite design) and carried out in the related pilot plant to tune the develop model. The designed variables in the experiments were Temperature, LHSV and pressure. However, the hydrogen over fresh feed ratio was remained constant. The ranges of these variables were respectively equal to 320-380ºC, 1- 21/hr and 50-55 bar. a power law kinetic model was also developed for our further research in the future .The rate order and activation energy , power of reactant concentration and frequency factor of this model was respectively equal to 1.4, 92.66 kJ/mol and k0=2.7*109 .
Abstract: In the present research, the titanium-catalyzed
ethylene dimerization and more specifically, the concomitant byproducts
and polymer formation have been studied in the presence of 2,5-dimethoxytetrahydrofuran as an electron donor compound in the
combination with triethylaluminium (TEA) as activator. Then, we
added ethylene chlorobromide as a new efficient promoter to the
relevant catalyst system. Finally, the behavior of novel homogeneous
[Titanium tetrabutoxide (Ti(OC4H9)4)/2,5-dimethoxytetrahydrofuran/
TEA/ethylene chlorobromide] was investigated in the various
operating conditions for the optimum production of 1-butene. In the
optimum conditions, a very high ethylene conversion (almost 90.77
%), a relative high selectivity to 1-butene (79.00 %), yield of reaction equal to 71.70 % and a significant productivity (turnover frequency
equal to 1370 h-1) were achieved.
Abstract: The antioxidant compounds are needed for the food, beverages, and pharmaceuticals industry. For this purpose, an appropriate method is required to measure the antioxidant properties in various types of samples. Spectrophotometric method usually used has some weaknesses, including the high price, long sample preparation time, and less sensitivity. Among the alternative methods developed to overcome these weaknesses is antioxidant biosensor based on superoxide dismutase (SOD) enzyme. Therefore, this study was carried out to measure the SOD activity originating from Deinococcus radiodurans and to determine its kinetics properties. Carbon paste electrode modified with ferrocene and immobilized SOD exhibited anode and cathode current peak at potential of +400 and +300mv respectively, in both pure SOD and SOD of D. radiodurans. This indicated that the current generated was from superoxide catalytic dismutation reaction by SOD. Optimum conditions for SOD activity was at pH 9 and temperature of 27.50C for D. radiodurans SOD, and pH 11 and temperature of 200C for pure SOD. Dismutation reaction kinetics of superoxide catalyzed by SOD followed the Lineweaver-Burk kinetics with D. radiodurans SOD KMapp value was smaller than pure SOD. The result showed that D. radiodurans SOD had higher enzyme-substrate affinity and specificity than pure SOD. It concluded that D. radiodurans SOD had a great potential as biological recognition component for antioxidant biosensor.
Abstract: In this work, biohydrogen production via dark
fermentation from alcohol wastewater using upflow anaerobic sludge
blanket reactors (UASB) with a working volume of 4 L was
investigated to find the optimum conditions for a maximum hydrogen
yield. The system was operated at different COD loading rates (23,
31, 46 and 62 kg/m3d) at mesophilic temperature (37 ºC) and pH 5.5.
The seed sludge was pretreated before being fed to the UASB system
by boiling at 95 ºC for 15 min. When the system was operated under
the optimum COD loading rate of 46 kg/m3d, it provided the
hydrogen content of 27%, hydrogen yield of 125.1 ml H2/g COD
removed and 95.1 ml H2/g COD applied, hydrogen production rate of
18 l/d, specific hydrogen production rate of 1080 ml H2/g MLVSS d
and 1430 ml H2/ L d, and COD removal of 24%.
Abstract: Cashew nut shells were converted into activated carbon powders using KOH activation plus CO2 gasification at 1027 K. The increase both of impregnation ratio and activation time, there was swiftly the development of mesoporous structure with increasing of mesopore volume ratio from 20-28% and 27-45% for activated carbon with ratio of KOH per char equal to 1 and 4, respectively. Activated carbon derived from KOH/char ratio equal to 1 and CO2 gasification time from 20 to 150 minutes were exhibited the BET surface area increasing from 222 to 627 m2.g-1. And those were derived from KOH/char ratio of 4 with activation time from 20 to 150 minutes exhibited high BET surface area from 682 to 1026 m2.g-1. The adsorption of Lead(II) and Cadmium(II) ion was investigated. This adsorbent exhibited excellent adsorption for Lead(II) and Cadmium(II) ion. Maximum adsorption presented at 99.61% at pH 6.5 and 98.87% at optimum conditions. The experimental data was calculated from Freundlich isotherm and Langmuir isotherm model. The maximum capacity of Pb2+ and Cd2+ ions was found to be 28.90 m2.g-1 and 14.29 m2.g-1, respectively.
Abstract: Rambutan is a tropical fruit which peel possesses antioxidant properties. This work was conducted to optimize extraction conditions of phenolic compounds from rambutan peel. Response surface methodology (RSM) was adopted to optimize subcritical water extraction (SWE) on temperature, extraction time and percent solvent mixture. The results demonstrated that the optimum conditions for SWE were as follows: temperature 160°C, extraction time 20min. and concentration of 50% ethanol. Comparison of the phenolic compounds from the rambutan peels in maceration 6h, soxhlet 4h, and SWE 20min., it indicated that total phenolic content (using Folin-Ciocalteu-s phenol reagent) was 26.42, 70.29, and 172.47mg of tannic acid equivalent (TAE) per g dry rambutan peel, respectively. The comparative study concluded that SWE was a promising technique for phenolic compounds extraction from rambutan peel, due to much more two times of conventional techniques and shorter extraction times.