Using Submerge Fermentation Method to Production of Extracellular Lipase by Aspergillus niger
In this study, lipase production has been investigated
using submerge fermentation by Aspergillus niger in Kilka fish oil as
main substrate. The Taguchi method with an L9 orthogonal array
design was used to investigate the effect of parameters and their
levels on lipase productivity. The optimum conditions for Kilka fish
oil concentration, incubation temperature and pH were obtained 3
gr./ml 35°C and 7, respectively. The amount of lipase activity in
optimum condition was obtained 4.59IU/ml. By comparing this
amount with the amount of productivity in the olive oil medium
based on the cost of each medium, it was that using Kilka fish oil is
84% economical. Therefore Kilka fish oil can be used as an
economical and suitable substrate in the lipase production and
industrial usages.
[1] H. Mala and et al.. "Mixed substrate solid state fermentation for
production and extraction of lipase from Aspergillusniger,MTCC 2594”.
J. Gen. Appl. Microbiol., 53, 2007,pp. 247–253.
[2] M.Sharma, "Aqueous two phase extraction – of Lipase from Rice Bran”,
Advances in Applied Science Research, 2, 2, 2011, pp. 265-271.
[3] R.Gupta, N.Gupta and R.Rathi, "Bactrial lipase: an overview of
production, purification and biochemical properties”,Applied Microbial
Biotechnology. 64, 6, 2004, pp. 763–781.
[4] A.Kumar and S. Kanwarm, "Lipase production in Solid-State
Fermentation (SSF): Recent Developments and Biotechnological
Applications”, Dynamic Biotechnology, Process Biotechnology and
Molecular biology., 6,2012, pp. 3-27.
[5] S.Basheer and et al., "Lipase from marine Aspergillusawamori
BTMFW032: Production, partial purification and application in oil
effluent treatment”, New Biotechnology, 28, 2011, pp. 627-638.
[6] A.Salihua and et al., "Lipase production: An insight in the utilization of
renewable agricultural residues”,Resources, Conservation and Recycling
58, 2012, pp.36– 44.
[7] K.Adinarayana and et al., "Optimization of process parameters for
production of lipase in solid-state fermentation by newly isolated
Aspergillus species”Indian Journal of biotechnology,2004, pp. 65-69.
[8] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V.
Gokhale, "Production of acidic lipase by a mutant of Aspergillus niger
NCIM 1207 in submerged”,fermentation Process Biochemistry, 39,
2004, pp. 2031-2034.
[9] G.Falony, J. C.Armas, J. C. D.Mendoza, J. L. M.Hernández,
"Production of extracellular lipase from Aspergillusniger by solid-state
fermentation”, Food Technol. Biotechnol, 44, 2006, pp. 235-240.
[10] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V.
Gokhale, "Production of acidic lipase by Aspergillusnigerin solid state
fermentation”, Process Biochemistry, 38, 5, 2002, pp. 715-721.
[11] T. Treichel and et al. "A Review on Microbial Lipases Production”,
Food Bioprocess Technology, 3,2010, pp.182–196.
[12] V. Mohanasrinivasan and et al., "A comparative study of the lipase yield
by solid state and submerged fermentations using fungal speciesfrom
biopharmaceutical oil waste”,African Journal of Biotechnology, 8, 1,
2009, pp. 073-076.
[13] Zh.Khoshkhoo And et al.,"Study on Changes of Protein and Lipid of
Fish Protein Concentrate (FPC) Produced form Kilkas in VP and MAP
Packages at Light and Darkness condition During Six Months”,
International Journal of Environmental Science and Development,1, 1,
2010, pp. 101-106.
[14] R. K.Roy, "Design of Experiments Using the Taguchi Approach”, 2001.
[15] M.Hosseinpour and et al., "Submerge Culture Studied for lipase
Production by Aspergillusniger NCIM 584 on soya flour”, Middle-East
Journal of Scientific Research, 7, 3, 2011, pp. 362-366.
[16] T.L.F.Pinheiro, "Produção de lipases porfermentaçãoemestadosólido e
fermentaçãosubmersautilizandoPenicilliumverrucosumcomomicrorganis
mo”, Erechim, RS, Brasil,2006, pp.45-47.
[17] M.L.Pera and et al., "Catalytic Properties of Lipase Extracts from
Aspergillus niger”, Food Technol. Biotechnol, 44, 2, 2006, pp.247–252
[1] H. Mala and et al.. "Mixed substrate solid state fermentation for
production and extraction of lipase from Aspergillusniger,MTCC 2594”.
J. Gen. Appl. Microbiol., 53, 2007,pp. 247–253.
[2] M.Sharma, "Aqueous two phase extraction – of Lipase from Rice Bran”,
Advances in Applied Science Research, 2, 2, 2011, pp. 265-271.
[3] R.Gupta, N.Gupta and R.Rathi, "Bactrial lipase: an overview of
production, purification and biochemical properties”,Applied Microbial
Biotechnology. 64, 6, 2004, pp. 763–781.
[4] A.Kumar and S. Kanwarm, "Lipase production in Solid-State
Fermentation (SSF): Recent Developments and Biotechnological
Applications”, Dynamic Biotechnology, Process Biotechnology and
Molecular biology., 6,2012, pp. 3-27.
[5] S.Basheer and et al., "Lipase from marine Aspergillusawamori
BTMFW032: Production, partial purification and application in oil
effluent treatment”, New Biotechnology, 28, 2011, pp. 627-638.
[6] A.Salihua and et al., "Lipase production: An insight in the utilization of
renewable agricultural residues”,Resources, Conservation and Recycling
58, 2012, pp.36– 44.
[7] K.Adinarayana and et al., "Optimization of process parameters for
production of lipase in solid-state fermentation by newly isolated
Aspergillus species”Indian Journal of biotechnology,2004, pp. 65-69.
[8] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V.
Gokhale, "Production of acidic lipase by a mutant of Aspergillus niger
NCIM 1207 in submerged”,fermentation Process Biochemistry, 39,
2004, pp. 2031-2034.
[9] G.Falony, J. C.Armas, J. C. D.Mendoza, J. L. M.Hernández,
"Production of extracellular lipase from Aspergillusniger by solid-state
fermentation”, Food Technol. Biotechnol, 44, 2006, pp. 235-240.
[10] N.D.Mahadik, U.S. Puntambekar, K.B. Bastawde, J.M. Khire and D.V.
Gokhale, "Production of acidic lipase by Aspergillusnigerin solid state
fermentation”, Process Biochemistry, 38, 5, 2002, pp. 715-721.
[11] T. Treichel and et al. "A Review on Microbial Lipases Production”,
Food Bioprocess Technology, 3,2010, pp.182–196.
[12] V. Mohanasrinivasan and et al., "A comparative study of the lipase yield
by solid state and submerged fermentations using fungal speciesfrom
biopharmaceutical oil waste”,African Journal of Biotechnology, 8, 1,
2009, pp. 073-076.
[13] Zh.Khoshkhoo And et al.,"Study on Changes of Protein and Lipid of
Fish Protein Concentrate (FPC) Produced form Kilkas in VP and MAP
Packages at Light and Darkness condition During Six Months”,
International Journal of Environmental Science and Development,1, 1,
2010, pp. 101-106.
[14] R. K.Roy, "Design of Experiments Using the Taguchi Approach”, 2001.
[15] M.Hosseinpour and et al., "Submerge Culture Studied for lipase
Production by Aspergillusniger NCIM 584 on soya flour”, Middle-East
Journal of Scientific Research, 7, 3, 2011, pp. 362-366.
[16] T.L.F.Pinheiro, "Produção de lipases porfermentaçãoemestadosólido e
fermentaçãosubmersautilizandoPenicilliumverrucosumcomomicrorganis
mo”, Erechim, RS, Brasil,2006, pp.45-47.
[17] M.L.Pera and et al., "Catalytic Properties of Lipase Extracts from
Aspergillus niger”, Food Technol. Biotechnol, 44, 2, 2006, pp.247–252
@article{"International Journal of Biological, Life and Agricultural Sciences:62540", author = "Masoumeh Ghasemi and Afshin Farahbakhsh and Arman Farahbakhsh and Ali Asghar Safari", title = "Using Submerge Fermentation Method to Production of Extracellular Lipase by Aspergillus niger", abstract = "In this study, lipase production has been investigated
using submerge fermentation by Aspergillus niger in Kilka fish oil as
main substrate. The Taguchi method with an L9 orthogonal array
design was used to investigate the effect of parameters and their
levels on lipase productivity. The optimum conditions for Kilka fish
oil concentration, incubation temperature and pH were obtained 3
gr./ml 35°C and 7, respectively. The amount of lipase activity in
optimum condition was obtained 4.59IU/ml. By comparing this
amount with the amount of productivity in the olive oil medium
based on the cost of each medium, it was that using Kilka fish oil is
84% economical. Therefore Kilka fish oil can be used as an
economical and suitable substrate in the lipase production and
industrial usages.
", keywords = "Lipase, Aspergillus niger, Kilka Fish oil, Submerge
Fermentation method.", volume = "8", number = "9", pages = "1000-4", }
