Abstract: Many high-risk pathogens that cause disease in
humans are transmitted through various food items. Food-borne
disease constitutes a major public health problem. Assessment of the
quality and safety of foods is important in human health. Rapid and
easy detection of pathogenic organisms will facilitate precautionary
measures to maintain healthy food. The Polymerase Chain Reaction
(PCR) is a handy tool for rapid detection of low numbers of bacteria.
We have designed gene specific primers for most common food
borne pathogens such as Staphylococci, Salmonella and E.coli.
Bacteria were isolated from food samples of various food outlets and
identified using gene specific PCRs. We identified Staphylococci,
Salmonella and E.coli O157 using gene specific primers by rapid and
direct PCR technique in various food samples. This study helps us in
getting a complete picture of the various pathogens that threaten to
cause and spread food borne diseases and it would also enable
establishment of a routine procedure and methodology for rapid
identification of food borne bacteria using the rapid technique of
direct PCR. This study will also enable us to judge the efficiency of
present food safety steps taken by food manufacturers and exporters.
Abstract: The ability to distinguish missense nucleotide
substitutions that contribute to harmful effect from those that do not
is a difficult problem usually accomplished through functional in
vivo analyses. In this study, instead current biochemical methods, the
effects of missense mutations upon protein structure and function
were assayed by means of computational methods and information
from the databases. For this order, the effects of new missense
mutations in exon 5 of PTEN gene upon protein structure and
function were examined. The gene coding for PTEN was identified
and localized on chromosome region 10q23.3 as the tumor
suppressor gene. The utilization of these methods were shown that
c.319G>A and c.341T>G missense mutations that were recognized in
patients with breast cancer and Cowden disease, could be pathogenic.
This method could be use for analysis of missense mutation in others
genes.
Abstract: Consumer demand for products with low fat or sugar content and low levels of food additives, as well as cost factors, make exopolysaccharides (EPS) a viable alternative. EPS remain an interesting tool to modulate the sensory properties of yoghurt. This study was designed to evaluate EPS production potential of commercial yoghurt starter cultures (Yo-Flex starters: Harmony 1.0, TWIST 1.0 and YF-L902, Chr.Hansen, Denmark) and their influence on an apparent viscosity of yoghurt samples. The production of intracellularly synthesized EPS by different commercial yoghurt starters varies roughly from 144,08 to 440,81 mg/l. Analysing starters’ producing EPS, they showed large variations in concentration and supposedly composition. TWIST 1.0 had produced greater amounts of EPS in MRS medium and in yoghurt samples but there wasn’t determined significant contribution to development of texture as well as an apparent viscosity of the final product. YF-L902 and Harmony 1.0 starters differed considerably in EPS yields, but not in apparent viscosities (p>0.05) of the final yoghurts. Correlation between EPS concentration and viscosity of yoghurt samples was not established in the study.
Abstract: This paper describes the optimization of a complex
dairy farm simulation model using two quite different methods of
optimization, the Genetic algorithm (GA) and the Lipschitz
Branch-and-Bound (LBB) algorithm. These techniques have been
used to improve an agricultural system model developed by Dexcel
Limited, New Zealand, which describes a detailed representation of
pastoral dairying scenarios and contains an 8-dimensional parameter
space. The model incorporates the sub-models of pasture growth and
animal metabolism, which are themselves complex in many cases.
Each evaluation of the objective function, a composite 'Farm
Performance Index (FPI)', requires simulation of at least a one-year
period of farm operation with a daily time-step, and is therefore
computationally expensive. The problem of visualization of the
objective function (response surface) in high-dimensional spaces is
also considered in the context of the farm optimization problem.
Adaptations of the sammon mapping and parallel coordinates
visualization are described which help visualize some important
properties of the model-s output topography. From this study, it is
found that GA requires fewer function evaluations in optimization
than the LBB algorithm.
Abstract: The aim of the study was to identify seat belt wearing
factor among road users in Malaysia. Evidence-based approach
through in-depth crash investigation was utilised to determine the
intended objectives. The objective was scoped into crashes
investigated by Malaysian Institute of Road Safety Research
(MIROS) involving passenger vehicles within 2007 and 2010. Crash
information of a total of 99 crash cases involving 240 vehicles and
864 occupants were obtained during the study period. Statistical test
and logistic regression analysis have been performed. Results of the
analysis revealed that gender, seat position and age were associated
with seat belt wearing compliance in Malaysia. Males are 97.6%
more likely to wear seat belt compared to females (95% CI 1.317 to
2.964). By seat position, the finding indicates that frontal occupants
were 82 times more likely to be wearing seat belt (95% CI 30.199 to
225.342) as compared to rear occupants. It is also important to note
that the odds of seat belt wearing increased by about 2.64% (95% CI
1.0176 to 1.0353) for every one year increase in age. This study is
essential in understanding the Malaysian tendency in belting up
while being occupied in a vehicle. The factors highlighted in this
study should be emphasized in road safety education in order to
increase seat belt wearing rate in this country and ultimately in
preventing deaths due to road crashes.
Abstract: This paper attempts to establish the fact that Multi
State Network Classification is essential for performance
enhancement of Transport protocols over Satellite based Networks. A
model to classify Multi State network condition taking into
consideration both congestion and channel error is evolved. In order
to arrive at such a model an analysis of the impact of congestion and
channel error on RTT values has been carried out using ns2. The
analysis results are also reported in the paper. The inference drawn
from this analysis is used to develop a novel statistical RTT based
model for multi state network classification.
An Adaptive Multi State Proactive Transport Protocol consisting
of Proactive Slow Start, State based Error Recovery, Timeout Action
and Proactive Reduction is proposed which uses the multi state
network state classification model. This paper also confirms through
detail simulation and analysis that a prior knowledge about the
overall characteristics of the network helps in enhancing the
performance of the protocol over satellite channel which is
significantly affected due to channel noise and congestion.
The necessary augmentation of ns2 simulator is done for
simulating the multi state network classification logic. This
simulation has been used in detail evaluation of the protocol under
varied levels of congestion and channel noise. The performance
enhancement of this protocol with reference to established protocols
namely TCP SACK and Vegas has been discussed. The results as
discussed in this paper clearly reveal that the proposed protocol
always outperforms its peers and show a significant improvement in
very high error conditions as envisaged in the design of the protocol.
Abstract: Wireless mobile communications have experienced
the phenomenal growth through last decades. The advances in
wireless mobile technologies have brought about a demand for high
quality multimedia applications and services. For such applications
and services to work, signaling protocol is required for establishing,
maintaining and tearing down multimedia sessions. The Session
Initiation Protocol (SIP) is an application layer signaling protocols,
based on request/response transaction model. This paper considers
SIP INVITE transaction over an unreliable medium, since it has been
recently modified in Request for Comments (RFC) 6026. In order to
help in assuring that the functional correctness of this modification is
achieved, the SIP INVITE transaction is modeled and analyzed using
Colored Petri Nets (CPNs). Based on the model analysis, it is
concluded that the SIP INVITE transaction is free of livelocks and
dead codes, and in the same time it has both desirable and
undesirable deadlocks. Therefore, SIP INVITE transaction should be
subjected for additional updates in order to eliminate undesirable
deadlocks. In order to reduce the cost of implementation and
maintenance of SIP, additional remodeling of the SIP INVITE
transaction is recommended.
Abstract: A new algorithm called Character-Comparison to Character-Access (CCCA) is developed to test the effect of both: 1) converting character-comparison and number-comparison into character-access and 2) the starting point of checking on the performance of the checking operation in string searching. An experiment is performed using both English text and DNA text with different sizes. The results are compared with five algorithms, namely, Naive, BM, Inf_Suf_Pref, Raita, and Cycle. With the CCCA algorithm, the results suggest that the evaluation criteria of the average number of total comparisons are improved up to 35%. Furthermore, the results suggest that the clock time required by the other algorithms is improved in range from 22.13% to 42.33% by the new CCCA algorithm.
Abstract: A specially designed flat plate was mounted vertically
over the axial line in the wind tunnel of the Aerospace Department of
the Pusan National University. The plate is 2 m long, 0.8 m high and 8
cm thick. The measurements were performed in velocity range from
15 to 60 m/s. A sand paper turbulizer was placed close to the plate nose
to provide fully developed turbulent boundary layer over the most part
of the plate. Strain balances were mounted in the trailing part of the
plate to measure the skin friction drag over removable insertions of
0.55×0.25m2 size. A set of the insertions was designed and
manufactured: 3mm thick polished metal surface and three compliant
surfaces. The compliant surfaces were manufactured of a silicone
rubber Silastic® S2 (Dow Corning company). To modify the
viscoelastic properties of the rubber, its composition was varied: 90%
of the rubber + 10% catalyst (standard), 92.5% + 7.5% (weak), 85% +
15% (strong). Modulus of elasticity and the loss tangent were
measured accurately for these materials in the frequency range from
40 Hz to 3 KHz using the unique proposed technique.
Abstract: The design requirements for successful human
accommodation in urban spaces are well known; and the range of
facilities available for meeting urban water quality and quantity
requirements is also well established. Their competing requirements
must be reconciled in order for urban spaces to be successful for
both. This paper outlines the separate human and water imperatives
and their interactions in urban spaces. Stormwater management
facilities- relative potential contributions to urban spaces are
contrasted, and design choices for achieving those potentials are
described. This study uses human success of urban space as the
evaluative criterion of stormwater amenity: human values call on
stormwater facilities to contribute to successful human spaces.
Placing water-s contribution under the overall idea of successful
urban space is an evolution from previous subjective evaluations.
The information is based on photographs and notes from
approximately 1,000 stormwater facilities and urban sites collected
during the last 35 years in North America and overseas, and the
author-s experience on multi-disciplinary design teams. This
conceptual study combines the disciplinary roles of engineering,
landscape architecture, and sociology in effecting successful urban
design.
Abstract: In This Article We establish moment inequality of
dependent random variables,furthermore some theorems of strong law
of large numbers and complete convergence for sequences of dependent
random variables. In particular, independent and identically
distributed Marcinkiewicz Law of large numbers are generalized to
the case of m0-dependent sequences.
Abstract: A virtual collaborative classroom was created at East Carolina University, using videoconference technology via regular internet to bring students from 18 different countries, 2 at a time, to the ECU classroom in real time to learn about each other-s culture. Students from two countries are partnered one on one, they meet for 4-5 weeks, and submit a joint paper. Then the same process is repeated for two other countries. Lectures and student discussions are managed with pre-determined topics and questions. Classes are conducted in English and reading assignments are placed on the website. Administratively all partners are independent, students pay fees and get credits at their home institution. Familiarity with technology, knowledge in cultural understanding and attitude change were assessed, only attitude changes are reported in this paper. After taking this course, all students stated their comfort level in working with, and their desire to interact with, culturally different others grew stronger and their xenophobia and isolationist attitudes decreased.
Abstract: The purpose of this paper is to consider the
introduction of online courses to replace the current classroom-based
staff training. The current training is practical, and must be
completed before access to the financial computer system is
authorized. The long term objective is to measure the efficacy,
effectiveness and efficiency of the training, and to establish whether
a transfer of knowledge back to the workplace has occurred. This
paper begins with an overview explaining the importance of staff
training in an evolving, competitive business environment and
defines the problem facing this particular organization. A summary
of the literature review is followed by a brief discussion of the
research methodology and objective. The implementation of the
alpha version of the online course is then described. This paper may
be of interest to those seeking insights into, or new theory regarding,
practical interventions of online learning in the real world.
Abstract: This study adopted previous fault patterns, results of
detection analysis, historical records and data, and experts-
experiences to establish fuzzy principles and estimate the failure
probability index of components of a power transformer. Considering
that actual parameters and limiting conditions of parameters may
differ, this study used the standard data of IEC, IEEE, and CIGRE as
condition parameters. According to the characteristics of each
condition parameter, relative degradation was introduced to reflect the
degree of influence of the factors on the transformer condition. The
method of fuzzy mathematics was adopted to determine the
subordinate function of the transformer condition. The calculation
used the Matlab Fuzzy Tool Box to select the condition parameters of
coil winding, iron core, bushing, OLTC, insulating oil and other
auxiliary components and factors (e.g., load records, performance
history, and maintenance records) of the transformer to establish the
fuzzy principles. Examples were presented to support the rationality
and effectiveness of the evaluation method of power transformer
performance conditions, as based on fuzzy comprehensive evaluation.
Abstract: In this paper an alternative visualisation approach of
the wake behind different vehicle body shapes with simplified and
fully-detailed underbody has been proposed and analysed. This
allows for a more clear distinction among the different wake regions.
This visualisation is based on a transformation of the cartesian
coordinates of a chosen wake plane to polar coordinates, using as
filter velocities lower than the freestream. This transformation
produces a polar wake plot that enables the division and
quantification of the wake in a number of sections. In this paper,
local drag has been used to visualise the drag contribution of the flow
by the different sections. Visually, a balanced wake can be observed
by the concentric behaviour of the polar plots. Alternatively,
integration of the local drag of each degree section as a ratio of the
total local drag yields a quantifiable approach of the wake uniformity,
where different sections contribute equally to the local drag, with the
exception of the wheels.
Abstract: The problem of exponential stability and periodicity for a class of cellular neural networks (DCNNs) with time-varying delays is investigated. By dividing the network state variables into subgroups according to the characters of the neural networks, some sufficient conditions for exponential stability and periodicity are derived via the methods of variation parameters and inequality techniques. These conditions are represented by some blocks of the interconnection matrices. Compared with some previous methods, the method used in this paper does not resort to any Lyapunov function, and the results derived in this paper improve and generalize some earlier criteria established in the literature cited therein. Two examples are discussed to illustrate the main results.
Abstract: In this paper we present a general formalism for the
establishment of the family of selective regressor affine projection
algorithms (SR-APA). The SR-APA, the SR regularized APA (SR-RAPA),
the SR partial rank algorithm (SR-PRA), the SR binormalized
data reusing least mean squares (SR-BNDR-LMS), and the SR normalized
LMS with orthogonal correction factors (SR-NLMS-OCF)
algorithms are established by this general formalism. We demonstrate
the performance of the presented algorithms through simulations in
acoustic echo cancellation scenario.
Abstract: Most electrical distribution systems are incurring large
losses as the loads are wide spread, inadequate reactive power
compensation facilities and their improper control. A typical static
VAR compensator consists of capacitor bank in binary sequential
steps operated in conjunction with a thyristor controlled reactor of the
smallest step size. This SVC facilitates stepless control of reactive
power closely matching with load requirements so as to maintain
power factor nearer to unity. This type of SVC-s requiring a
appropriately controlled TCR. This paper deals with an air cored
reactor suitable for distribution transformer of 3phase, 50Hz, Dy11,
11KV/433V, 125 KVA capacity. Air cored reactors are designed,
built, tested and operated in conjunction with capacitor bank in five
binary sequential steps. It is established how the delta connected TCR
minimizes the harmonic components and the operating range for
various electrical quantities as a function of firing angle is
investigated. In particular firing angle v/s line & phase currents, D.C.
components, THD-s, active and reactive powers, odd and even triplen
harmonics, dominant characteristic harmonics are all investigated and
range of firing angle is fixed for satisfactory operation. The harmonic
spectra for phase and line quantities at specified firing angles are
given. In case the TCR is operated within the bound specified in this
paper established through simulation studies are yielding the best
possible operating condition particularly free from all dominant
harmonics.
Abstract: This present paper proposes the modified Elastic Strip
method for mobile robot to avoid obstacles with a real time system in
an uncertain environment. The method deals with the problem of
robot in driving from an initial position to a target position based on
elastic force and potential field force. To avoid the obstacles, the
robot has to modify the trajectory based on signal received from the
sensor system in the sampling times. It was evident that with the
combination of Modification Elastic strip and Pseudomedian filter to
process the nonlinear data from sensor uncertainties in the data
received from the sensor system can be reduced. The simulations and
experiments of these methods were carried out.
Abstract: Many well-known interconnection networks, such as kary n-cubes, recursive circulant graphs, generalized recursive circulant graphs, circulant graphs and so on, are shown to belong to the family of cycle composition networks. Recently, various studies about mutually independent hamiltonian cycles, abbreviated as MIHC-s, on interconnection networks are published. In this paper, using an improved construction method, we obtain MIHC-s on cycle composition networks with a much weaker condition than the known result. In fact, we established the existence of MIHC-s in the cycle composition networks and the result is optimal in the sense that the number of MIHC-s we constructed is maximal.