Abstract: Carbohydrate can be used as a substrate that can be consumed by C. butyricum and converted to useful chemicals such as acetic and butyric acid. Influence of concentration and types of carbohydrate to cell growth, carbohydrate consumed, productivity and carbon balance have been explored. Batch reactor was selected in this study to avoid contamination due to simpler operation system. Glucose was preferred as first types of carbohydrate to be tested. Six concentrations were studied from 0 to 28g/L. Eventually, 15g/L has shown the best concentration for glucose in term of growth rate (2.63h-1) and carbon balance (99.76% recovery). Comparison for types of carbohydrate was also conducted. 15g/L of xylose (monosaccharide) and starch (complex carbohydrate) was tested. In term of growth rate and productivity, glucose showed the best carbohydrates. Results for this study showed that glucose and xylose produced more than 80% of acetic acid and less than 20% of butyric acid. Meanwhile, 63.1% of acetic acid and 36.9% of butyric acid were produced from starch.
Abstract: One of the major pollutants in the environment is arsenic (As). Due to the toxic effects of As to all organisms, its remediation is necessary. Conventional technologies used in the remediation of As contaminated soils are expensive and may even compromise the structure of the soil. An attractive alternative is phytoremediation, which is the use of plants which can take up the contaminant in their tissues. Plant growth promoting bacteria (PGPB) has been known to enhance growth of plants through several mechanisms such as phytohormone production, phosphate solubilization, siderophore production and 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, which is an essential trait that aids plants especially under stress conditions such as As stress. Twenty one bacteria were isolated from As-contaminated soils in the vicinity of the Janghang Smelter in Chungnam Province, South Korea. These exhibited high tolerance to either arsenite (As III) or arsenate (As V) or both. Most of these isolates possess several plant growth promoting traits which can be potentially exploited to increase phytoremediation efficiency. Among the identified isolates is Pseudomonas sp. JS1215, which produces ACC deaminase, indole acetic acid (IAA), and siderophore. It also has the ability to solubilize phosphate. Inoculation of JS1215 significantly enhanced root and shoot length and biomass accumulation of maize under normal conditions. In the presence of As, particularly in lower As level, inoculation of JS1215 slightly increased root length and biomass. Ethylene increased with increasing As concentration, but was reduced by JS1215 inoculation. JS1215 can be a potential bioinoculant for increasing phytoremediation efficiency.
Abstract: A new and cost effective RP-HPLC method was
developed and validated for simultaneous analysis of non steroidal
anti inflammatory dugs Diclofenac sodium (DFS), Flurbiprofen
(FLP) and an opioid analgesic Tramadol (TMD) in advanced drug
delivery systems (Liposome and Microcapsules), marketed brands
and human plasma. Isocratic system was employed for the flow of
mobile phase consisting of 10 mM sodium dihydrogen phosphate
buffer and acetonitrile in molar ratio of 67: 33 with adjusted pH of
3.2. The stationary phase was hypersil ODS column (C18, 250×4.6
mm i.d., 5 μm) with controlled temperature of 30 C°. DFS in
liposomes, microcapsules and marketed drug products was
determined in range of 99.76-99.84%. FLP and TMD in
microcapsules and brands formulation were 99.78 - 99.94 % and
99.80 - 99.82 %, respectively. Single step liquid-liquid extraction
procedure using combination of acetonitrile and trichloroacetic acid
(TCA) as protein precipitating agent was employed. The detection
limits (at S/N ratio 3) of quality control solutions and plasma samples
were 10, 20, and 20 ng/ml for DFS, FLP and TMD, respectively.
The Assay was acceptable in linear dynamic range. All other
validation parameters were found in limits of FDA and ICH method
validation guidelines. The proposed method is sensitive, accurate and
precise and could be applicable for routine analysis in
pharmaceutical industry as well as in human plasma samples for
bioequivalence and pharmacokinetics studies.
Abstract: The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
Abstract: Calcium magnesium acetate (CMA) is environmentally
benign deicing chemicals that can replace sodium chloride that is
widely used on roads and highways at present for snow and ice control
to provide safe driving conditions during winter. The price of CMA
from petroleum-derived acetic acid is quite expensive. The bamboo
vinegar is the by-product from bamboo charcoal production. The
bamboo vinegar was used to prepare calcium acetate as raw materials,
and its deicing and corrosive performances were studied in this paper.
The results show that the freezing temperature of calcium acetate is
lower than that of sodium chloride when they have same molar
concentration, the deicing performance of calcium acetate is better
than that of sodium chloride when they have same moles, while the
deicing performance of sodium chloride is better than that of calcium
acetate. The corrosion of sodium chloride on iron-nail and steel-nail is
larger than that of calcium acetate whether they have same mass
concentration or same molar concentration, and the corrosion of
sodium chloride and calcium acetate on iron-nail is larger than that on
steel-nail, and calcium acetate almost hasn't corrosion on steel-nail.
Abstract: The studying of enzymatic esterification of carboxylic
acids and higher alcohols was performed by esterase Saccharomyces
cerevisiae in water-organic medium. Investigation of the enzyme
specificity to acetic substrates showed the best result with acetic acid
in esterification reactions with ethanol whereas within other
carboxylic acids the esterification decreased with acids: hexanoic >
pentanoic > butyric > decanoic. In relation to higher alcohols C3-C5,
esterification increased with alcohols propanol < butanol < amylol.
Also it was determined that esterase was more specific to alcohols
with branched chain such as isobutyl alcohol and isoamyl alcohol.
Data obtained may have important practical implications, for
example, for application of yeast esterase in producing various
volatile esters as well as in enzymatic transformation of volatile acids
and toxic fusel alcohols into volatile esters by providing the
production of the high quality alcoholic beverages with redused
content of higher alcohols as well as with improved degustational and
hygienic properties.
Abstract: Corporate identity, which has several advantages such
that the employees become integrated with their corporations,
corporation is distinguished from its competitors and it is recognized
by the masses, is the total of the distinctive corporate features that
and corporation has. That the information takes part in production as
a more important component than labor and capital has required that
the corporations are reorganized as information-based. Therefore,
information and its management have reached a basic and prevalent
position in having sustainable competitive advantage. Thanks to the
information management which regulates the information and makes
it reachable and available, information will be produced in line with a
specific purpose in the corporations and be used in all the corporate
processes. As an auxiliary power for increase in the economic
potential, efficiency and productivity of the corporation, corporate
identity consists of four components. These are corporate philosophy,
corporate design, corporate behavior and corporate communication.
In this study, the effects of the information management on corporate
identity are discussed from the point of these four elements.
Abstract: Twenty - nine Holstein cows were used to evaluate the effects of different dry period (DP) lengths on milk yield and composition, some blood metabolites, and complete blood count (CBC). Cows were assigned to one of 2 treatments: 1) 60-d dry period, 2) 35-d DP. Milk yield, from calving to 60 days, was not different for cows on the treatments (p =0.130). Cows in the 35-d DP produced more milk protein and SNF compare with cows in treatment 1 (p ≤ 0.05). Serum glucose, non-esterified fatty acids (NEFA), beta hydroxyl butyrate acid (BHBA), blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body condition score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. The results of this study demonstrated we can reduce the dry period length to 35 days with no problems.
Abstract: Recognizing human action from videos is an active
field of research in computer vision and pattern recognition. Human
activity recognition has many potential applications such as video
surveillance, human machine interaction, sport videos retrieval and
robot navigation. Actually, local descriptors and bag of visuals words
models achieve state-of-the-art performance for human action
recognition. The main challenge in features description is how to
represent efficiently the local motion information. Most of the
previous works focus on the extension of 2D local descriptors on 3D
ones to describe local information around every interest point. In this
paper, we propose a new spatio-temporal descriptor based on a spacetime
description of moving points. Our description is focused on an
Accordion representation of video which is well-suited to recognize
human action from 2D local descriptors without the need to 3D
extensions. We use the bag of words approach to represent videos.
We quantify 2D local descriptor describing both temporal and spatial
features with a good compromise between computational complexity
and action recognition rates. We have reached impressive results on
publicly available action data set
Abstract: Vinegar or sour wine is a product of alcoholic and
subsequent acetous fermentation of sugary precursors derived from
several fruits or starchy substrates. This delicious food additive and
supplement contains not less than 4 grams of acetic acid in 100 cubic
centimeters at 20°C. Among the large number of bacteria that are
able to produce acetic acid, only few genera are used in vinegar
industry most significant of which are Acetobacter and
Gluconobacter. In this research we isolated and identified an
Acetobacter strain from Iranian apricot, a very delicious and sensitive
summer fruit to decay, we gathered from fruit's stores in Isfahan,
Iran. The main culture media we used were Carr, GYC, Frateur and
an industrial medium for vinegar production. We isolated this strain
using a novel miniature fermentor we made at Pars Yeema
Biotechnologists Co., Isfahan Science and Technology Town (ISTT),
Isfahan, Iran. The microscopic examinations of isolated strain from
Iranian apricot showed gram negative rods to cocobacilli. Their
catalase reaction was positive and oxidase reaction was negative and
could ferment ethanol to acetic acid. Also it showed an acceptable
growth in 5%, 7% and 9% ethanol concentrations at 30°C using
modified Carr media after 24, 48 and 96 hours incubation
respectively. According to its tolerance against high concentrations of
ethanol after four days incubation and its high acetic acid production,
8.53%, after 144 hours, this strain could be considered as a suitable
industrial strain for a production of a new type of vinegar, apricot
vinegar, with a new and delicious taste. In conclusion this is the first
report of isolation and identification of an Acetobacter strain from
Iranian apricot with a very good tolerance against high ethanol
concentrations as well as high acetic acid productivity in an
acceptable incubation period of time industrially. This strain could be
used in vinegar industry to convert apricot spoilage to a beneficiary
product and mentioned characteristics have made it as an amenable
strain in food and agricultural biotechnology.
Abstract: To calculate the temperature distribution of the slab in
a hot rolled reheating furnace a mathematical model has been
developed by considering the thermal radiation in the furnace and
transient conduction in the slab. The furnace is modeled as radiating
medium with spatially varying temperature. Radiative heat flux within
the furnace including the effect of furnace walls, combustion gases,
skid beams and buttons is calculated using the FVM and is applied as
the boundary condition of the transient conduction equation of the
slab. After determining the slab emissivity by comparison between
simulation and experimental work, variation of heating characteristics
in the slab is investigated in the case of changing furnace temperature
with various time and the slab residence time is optimized with this
evaluation.
Abstract: One of the most challenges for hard surface cleaning product is to get rid of soap scum, a filmy sticky layer in the bathroom. The deposits of soap scum can be removed by using a proper surfactant solution with chelating agent. Unfortunately, the conventional chelating agent, ethylenediamine tetraacetic acid (EDTA), has low biodegradability, which can be tolerance in water resources and harmful to aquatic animal and microorganism. In this study, two biodegradable chelating agents, ethylenediamine disuccinic acid (EDDS) and glutamic acid diacetic acid (GLDA) were introduced as a replacement of EDTA. The result shows that using GLDA with amphoteric surfactant gave the highest equilibrium solubility of soap scum.
Abstract: This study was conducted to evaluate the effects of
gibberellic acid and 2,4- dichlorophenoxyacetic acid on flower
number, fruit growth and fruit quality of wax apple. GA3 and 2,4-D
were applied at small bud and petal fall stage. Number of flower,
fruit set, fruit drop, fruit crack, fruit growth and fruit quality were
recorded. Results indicated that spraying with 10 ppm GA3 had the
best results in number of flower. GA3 spray at 30 ppm gave the
faster rate of fruit growth than the other treatments. Fruit set, fruit
size as well as fruit weight markedly improved by spraying 30 ppm
GA3, followed by 10 ppm GA3 compared to untreated control.
Moreover, spray GA3 at 30 ppm was the most effective and
increased total soluble solids, reduced titratable acidity and fruit
drop. On the other hand, it was noticed that with 10 ppm 2,4-D
application also enhanced the fruit growth rate, improved
physiological and biochemical characters of fruit compared to
untreated control. It was concluded that both GA3 and 2,4-D spray
have positive effects on fruit development, reduced fruit drop, fruit
crack and improved fruit quality of wax apple under field conditions.
Abstract: The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.
Abstract: Vinegar is a precious food additive and complement as well as effective preservative against food spoilage. Recently traditional vinegar production has been improved using various natural substrates and fruits such as grape, palm, cherry, coconut, date, sugarcane, rice and balsam. These neoclassical fermentations resulted in several vinegar types with different tastes, fragrances and nutritional values because of applying various acetic acid bacteria as starters. Acetic acid bacteria include genera Acetobacter, Gluconacetobacter and Gluconobacter according to latest edition of Bergy-s Manual of Systematic Bacteriology that classifies genera on the basis of their 16s RNA differences. Acetobacter spp as the main vinegar starters belong to family Acetobacteraceae that are gram negative obligate aerobes, chemoorganotrophic bacilli that are oxidase negative and oxidize ethanol to acetic acid. In this research we isolated and identified a native Acetobacter strain with high acetic acid productivity and tolerance against high ethanol concentrations from Iranian peach as a summer delicious fruit that is very susceptible to food spoilage and decay. We used selective and specific laboratorial culture media such as Standard GYC, Frateur and Carr medium. Also we used a new industrial culture medium and a miniature fermentor with a new aeration system innovated by Pars Yeema Biotechnologists Co., Isfahan Science and Technology Town (ISTT), Isfahan, Iran. The isolated strain was successfully cultivated in modified Carr media with 2.5% and 5% ethanol simultaneously in high temperatures, 34 - 40º C after 96 hours of incubation period. We showed that the increase of ethanol concentration resulted in rising of strain sensitivity to high temperature. In conclusion we isolated and characterized a new Acetobacter strain from Iranian peach that could be considered as a potential strain for production of a new vinegar type, peach vinegar, with a delicious taste and advantageous nutritional value in food biotechnology and industrial microbiology.
Abstract: Ferroresonance is an electrical phenomenon in
nonlinear character, which frequently occurs in power system due to
transmission line faults and single or more-phase switching on the
lines as well as usage of the saturable transformers. In this study, the
ferroresonance phenomena are investigated under the modeling of the
West Anatolian Electric Power Network of 380 kV in Turkey. The
ferroresonance event is observed as a result of removing the loads at
the end of the lines. In this sense, two different cases are considered.
At first, the switching is applied at 2nd second and the ferroresonance
affects are observed between 2nd and 4th seconds in the voltage
variations of the phase-R. Hence the ferroresonance and nonferroresonance
parts of the overall data are compared with each
others using the Fourier transform techniques to show the
ferroresonance affects.
Abstract: This experiment was performed with the purpose of
investigating effect of additional blend of probiotics Saccharomyces
cerevisiae and Lactobacillus acidophilus on plasma fatty acid profiles
particularly conjugated linoleic acid (CLA) in growing goats fed corn
silage, and selected the optimal levels of the probiotics for further study.
Twenty-four growing crossbred (Thai native x Anglo-Nubian) goats that
weighed (14.2 ± 2.3) kg, aged about 6 months, were purchased and
allocated to 4 treatments according to Randomized Complete Block
Design (RCBD) with 6 goats in each treatment. The blocks were made by
weight into heavy, medium, and light goats and each of the treatments
contained two goats from each of the blocks. In the mean time, ruminal
average pH unaffected, but the NH3-N and also plasma urea nitrogen
(p0.05) were raised, but propionic
proportion (p0.05) were reduced in
concurrent with raise of acetic proportion and resultantly C2:C3 ratio
(p>0.05). On plasma fatty acid profiles, total saturated fatty acids
(p>0.05) was increased, and contrasted with decrease of C15:0
(p0.05), and C18-C22 polyunsaturated fatty acids
(p
Abstract: The degradation of selected pharmaceuticals in some
water matrices was studied by using several chemical treatments. The
pharmaceuticals selected were the beta-blocker metoprolol, the
nonsteroidal anti-inflammatory naproxen, the antibiotic amoxicillin,
and the analgesic phenacetin; and their degradations were conducted
by using UV radiation alone, ozone, Fenton-s reagent, Fenton-like
system, photo-Fenton system, and combinations of UV radiation and
ozone with H2O2, TiO2, Fe(II), and Fe(III). The water matrices, in
addition to ultra-pure water, were a reservoir water, a groundwater,
and two secondary effluents from two municipal WWTP. The results
reveal that the presence of any second oxidant enhanced the
oxidation rates, with the systems UV/TiO2 and O3/TiO2 providing the
highest degradation rates. It is also observed in most of the
investigated oxidation systems that the degradation rate followed the
sequence: amoxicillin > naproxen > metoprolol > phenacetin. Lower
rates were obtained with the pharmaceuticals dissolved in natural
waters and secondary effluents due to the organic matter present
which consume some amounts of the oxidant agents.
Abstract: Processing tabah bamboo shoot as fermented pickle is
one of the way to increase the shelf life of this bamboo shoot. The
advantage of this shoot is low concentration of hydro cyanic acid
(HCN) make it potential for functional food product. This study
aimed to determine the characteristic of tabah bamboo shoot pickle
such as total of lactic acid bacteria (LAB), pH, total acidity, and
hydro cyanic acid (HCN) content, and also find the LAB’s type
involved during fermentation, and organic acids’ profiles. The pickle
was made by natural fermentation with 6% salt concentration and
fermentation conducted for 13 days.
The result showed during the fermentation time, in the 4th day
LAB’s number was highest as much as 72 x 107 CFU/ml and the
lowest pH was 3.09. We also found decreasing in HCN from 37.8
ppm at the beginning to 20.52 ppm at the end of fermentation
process. The organic acids detected during the fermentation were
lactic acid with the highest concentration was 0.0546 g/100 g and
small amount of acetic acid. By using PCR method, the 18 of LABs
which had rod shape were detected as member of Lactobacillus spp.,
in which 17 strains detected as L. plantarum.
Abstract: An HPLC-UV analytical method was developed to
determine ethylenediaminetetraacetic acid (EDTA) in dairy
wastewater and surface water. The optimizing separation was achieved
by reversed–phase ion-pair liquid chromatography on a C18 column
using methanol as mobile phase solvent, tetrabutylammonium bromide
as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate
of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca,
Mg or NO3
- was detected. Method performance was evaluated in terms
of linearity, repeatability and reproducibility. The method detection
limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72 ~
261 μg L-1 at a large dairy site. A change of EDTA concentration was
observed downstream of the dairy effluent discharge, but this was well
under the predicted no effect concentration for aquatic ecosystem.