Effect of Gibberellic Acid and 2,4- Dichlorophenoxyacetic Acid on Fruit Development and Fruit Quality of Wax Apple

This study was conducted to evaluate the effects of gibberellic acid and 2,4- dichlorophenoxyacetic acid on flower number, fruit growth and fruit quality of wax apple. GA3 and 2,4-D were applied at small bud and petal fall stage. Number of flower, fruit set, fruit drop, fruit crack, fruit growth and fruit quality were recorded. Results indicated that spraying with 10 ppm GA3 had the best results in number of flower. GA3 spray at 30 ppm gave the faster rate of fruit growth than the other treatments. Fruit set, fruit size as well as fruit weight markedly improved by spraying 30 ppm GA3, followed by 10 ppm GA3 compared to untreated control. Moreover, spray GA3 at 30 ppm was the most effective and increased total soluble solids, reduced titratable acidity and fruit drop. On the other hand, it was noticed that with 10 ppm 2,4-D application also enhanced the fruit growth rate, improved physiological and biochemical characters of fruit compared to untreated control. It was concluded that both GA3 and 2,4-D spray have positive effects on fruit development, reduced fruit drop, fruit crack and improved fruit quality of wax apple under field conditions.

Authors:

• Nguyen Minh Tuan
• Yen Chung–Ruey

Keywords:

• Wax apple
• GA3
• 2
• 4-D
• fruit growth
• fruit quality.

References:

[1] M. Agusti, V. Almela, I. Andreu, M. Juan, and L. Zacarias, "Synthetic
auxin 3,5,6-TPA promotes fruit development and climacteric in Prunus
persica L. Batsch," J. Hort. Sci. Biotech, 74, pp 556-660. 1999.
[2] M. Agusti, M.E. Otmani, M. Juan, and V. Almela, "Effect of 3,5,6-
trichloro-2-pyridyloxyacetic acid on clementine early fruitlet
development and on fruit size at maturity," J. Hort. Sci, 70, pp 955-962.
1995.
[3] M. Agusti, M. Juan, V. Almela, and C. Speroni, "The effect of 2,4-DP
on fruit development in apricots (Prunus armeniaca L.)," Scientia Hort,
57, pp 51- 57. 1994.
[4] I. Almeida, I.M. Leite, J.D. Rodrigues, and E.O. Ono, "Application of
plant growth regulators at pre-harvest for fruit development of ÔÇÿPERA-
oranges," Braz. Arch. Biol. Technol, 47, pp 658-662. 2004.
[5] R.N. Arteca, "Plant Growth Substances: Principles and Applications,"
Chapman and Hall Press, New York, USA, 332 pp, 1996.
[6] A. Basak, E. Rozpara, and Z. Grzyb, "Use of Bioregulators to Reduce
Sweet Cherry Tree Growth and to improve Fruit Quality," Acta
Horticult, 468, pp 719-723. 1998.
[7] B.S. Baghel and D.A. Sarnaik, "Effect of 2,4-D on yield and physicchemical
composition of mrigbahar sweet orange variety, Poona
Mosambi," South Indian Hort, 33, pp 395-396. 1985.
[8] G.H.V.R. Babu and M.L. Lavaniya, "Effect of plant growth regulators
on fruit set and fruit drop of Pant lemon-1," Indian J. Hort, 42, pp 237-
240. 1985.
[9] W. Baogang, W. Jianhui, and L. Hao, "Reduced chilling injury in
mango fruit by 2,4-dichlorophenoxyacetic acid and the antioxidant
response," Postharvest Biology and Technology, 48(2), pp 172-181.
2008.
[10] D.R. Bhattarai and D. M. Gautam, "Effect of harvesting method and
calcium on post harvest physiology of tomato," Nepal Agriculture
Research Journal, 7, pp 37-41. 2006.
[11] J.C. Chang, and T.S. Lin, "GA3 increases fruit weight in ÔÇÿYu Her Pau-
litchi," Sci. Horticult, 108(4), pp 442-443. 2006.
[12] C.T. Chao and C.J. Lovatt, "Foliar applied 3,5,6-trichloro-2-
pyridoxylacetic acid (3,5,6-TPA) increases yield of commercially
valuable large-size fruit of "Fina Sodea" clementine mandarin,"
Acta Hort, 884, pp 433-440. 2010
[13] M. Clayton, W.V. Biasi, I.T. Agar, S.M. Southwick, and E.J. Mitcham,
"Postharvest Quality of ÔÇÿBing- Cherries Following Preharvest
Treatment with Hydrogen Cyanamide, Calcium Ammonium Nitrate, or
Gibberellic Acid," Hort. Sci, 38(3), pp 407-411. 2003.
[14] M. Clayton, W.V. Biasi, I.T. Agar, S.M. Southwick, E.J. Mitcham,
"Sensory quality of ÔÇÿBing- sweet cherries following preharvest treatment
with hydrogen cyanamide, calcium ammonium nitrate, or gibberellic
acid," HortScience, 41, pp 745-748. 2006.
[15] J.A. Cline and M. Trought, "Effect of Gibberellic Acid on Fruit
Cracking and Quality of Bing and Sam Sweet Cherries," Can. J. Plant
Sci, 87(3), pp 545-550. 2007.
[16] F.S. Davies and G. Zalman, "Gibberellic acid, fruit freezing, and postfreeze
quality of Hamlin oranges," Hort. Tech, 16(2), pp 301-305. 2006.
[17] F.S. Davies, "Growth regulator improvement of post-harvest quality,"
In: Fresh citrus fruit. W. Wardowski, S. Nagy, W. Grieson (eds.), AVI
Press, Westport, Conn 77-99 pp. 1986.
[18] Z.A. Dawood, "Studies into fruit splitting and quality of sweet cherry
(Prunus avium L.), Tomato (Lycopersicon esculentum L.), and grape
(Vitis vinifera L.)," Ph.D thesis. Department of Horticulture, Wye
College, University of London, Wye, UK ,256 pp. 1986.
[19] T. Deckers and H. Schoofs, "Improvement of fruit set on young pear
trees cultivar Conference with gibberellins," Acta Hort, 596, pp 735-
743. 2002.
[20] L.K. Demirsoy and S.Bilgener, "The Effects of Preharvest Calcium
Hydroxide Applications on Cracking and Fruit Quality in 0900 Ziraat,
Lambert and Van Sweet Cherry Varieties," Acta Horticult, 468, pp 657-
662. 1998.
[21] E. Dorcey, C. Urbez, M.A. Blazquez, J.Carbonell, and M.A. Perez-
Amador, "Fertilization dependent auxin response in ovules triggers
fruit development through the modulation of gibberellin metabolism
in Arabidopsis," Plant Journal, 58, pp 318-332. 2009.
[22] C. Elisa, L. Lucia, S. Oriana, P. Tiziana, S. Angelo, B. Mezzetti, "Auxin
synthesis-encoding transgene enhances grape fecundity," Plant
Physiology, 143(4), pp 1689- 1694. 2007.
[23] A.M.A. El-Sese, "Effect of gibberellic acid 3 (GAs) on yield and fruit
characteristics of Balady mandarin," Assiut. J. Agri. Sci, 36, pp 23-35.
2005.
[24] A.A. Eman, M.M.M.A. El-moneim, O.A. El- Migeed, and M.M. Ismail,
"GA3 and Zinc Sprays for Improving Yield and Fruit Quality of
Washington Navel Orange Trees Grown under Sandy Soil
Conditions," Res. J. Agric. Biol. Sci, 3(5), pp 498-503. 2007.
[25] S.H.M.M. El-Sharkawy and S.M.A. Mehaisen, "Effect of gibberellin
and potassium foliage sprays on productivity and fruit quality of guava
trees," Egypt J. Appl. Sci, 20(3), pp 151-162. 2005.
[26] S. Horvitz, C. Godoy, A.F. Lopez Camelo, A. Yommi, "Application of
Gibberellic Acid to ÔÇÿSweetheart- Sweet Cherries:Effect on Fruit Quality
at Harvest and During Cold Storage," Acta Horticult, 628, pp 311-316.
2003.
[27] J.H. Huang and L. Huang, "The application of GA3 in citrus orchards,"
South China Fruits, 3, pp 32-36. 2005.
[28] F. Kappel and R. MacDonald, "Early gibberellic acid spray increase
fruiting and fruit size of Sweetheart sweet cherry," J. Am. Pomol. Soc,
61(1), pp 38-43. 2007.
[29] H.A. Kassem, A.M. El-Kobbia, H.A. Marzouk, M.M. El- Sebaiey,
"Effect of foliar sprays on fruit retention, quality and yield of Costata
persimmon trees," Emir. J. Food Agric, 22 (4), pp 259-274. 2010.
[30] M.M. Khandaker, A.N. Boyce, and N. Osman, "The influence of
hydrogen peroxide on the growth, development and quality of wax apple
(Syzygium samarangense, [Blume] Merrill & L.M. Perry var. jambu
madu) fruits," Plant Physiology and Biochemistry, 53, pp 101-110.
2012.
[31] M.M.A. Khan, A.C. Gautam, F. Mohammad, M.H. Siddiqui, M. Naeem,
and M.N. Khan, "Effect of gibberellic acid spray on performance of
tomato," Turk. J. Biol, 30, pp 11-16. 2006.
[32] N.M.A. Khassawneh, N.S. Karam, and R.A. Shibli, "Growth and
flowering of black iris (Iris nigricans Dinsm.) following treatment with
plant growth regulators," Scientia Horticulturae, 107, pp 187-193. 2006.
[33] S. Kondo and N. Mizuno, "Relation between early drop of apple fruit
and endogenous growth regulators and effects of MCPB, GA3 plus GA4
and BA sprays on fruit abscission," Journal of Japanese Society for
Horticultural Science, 58, pp 9-16. 1989.
[34] K. Mengel and E.A. Kirkby, "Principles of Plant Nutrition," 4th End.,
International Potash Institute, Bern, Switzerland, 687 pp. (1987).
[35] J. Morton, "Loquat," In: Morton JF (Ed.). Fruits of Warm Climates.
Miami FL. Inc. Winter vile, NC, 103-108 pp. 1987.
[36] M.A. Nawaz, W. Ahmad, S. Ahmad, and M.M. Khan, "Role of growth
regulators on preharvest fruit drop," Pak. J. Bot, 40(5), pp 1971-1981.
2008.
[37] M.E. Otmani, A. Ait-Oubahou, C.J. Lovatt, F. El-Hassainate, "Effect of
Gibberellic acid, Urea and KNO3 on yield, composition and nutritional
quality of Clementine mandarin fruit juice," ISHS Acta Horticulturae,
632. 2004. XXVI International Horticultural Congress: Citrus and other
subtropical and tropical fruit crops: issues, advances and opportunities.
[38] G. Paroussi, D.G. Voyiatzis, E. Paroussi, P.D. Drogour, "Growth,
flowering and yield responses to GA3 of strawberry grown under
different environmental conditions," Scientia Hortic, 96, pp 103-113.
2002.
[39] E.J.P. Pires, M.M. Terra, C.V. Pommer, I.R.S. Passos, "Improvement of
cluster and berry quality of Centennial Seedless grapes through
gibberellic acid," Acta Horticulturae, 526, pp 293-299. 2000
[40] R. Ranjan, S.S. Purohit, V. Prasad, "Plant Hormones: Action and
Application," Agrobios, India, 2003. pp183-189
[41] G.H.V. Ratnababu, M.L. Lavania, K.K. Misra, "Effect of plant growth
regulator sprays on yield and physic-chemical composition of Pant
lemon-1 (Citrus limon Burm.) fruits in the off-season flush," Prog. Hort,
16 (3-4), pp 191-198. 1984.
[42] A. Sepahi, "GA3 concentration for controlling fruit cracking in
pomegranates," Iran Agric. Res, 5, pp 93-99. 1986.
[43] J.C. Serrani, R. Sanjuán , O. Ruiz-Rivero , M. Fos , J.L. Garcia-
Martinez, "Gibberellin regulation of fruit set and growth in tomato,"
Plant Physiology, 145, pp 922-934. 2007.
[44] Z.H. Sh├╝, C.C. Chu, L.C. Hwang, C.S. Shieh, "Light, temperature, and
sucrose affect color, diameter, and soluble solids of disks of wax apple
fruit skin," HortScience, 36, pp 279-281. 2001.
[45] A. Singh and J.N. Singh, "Studies on the influence of biofertilizers and
bioregulators on flowering, yield and fruit quality of strawberry Cv.
Sweet Charlie," Ann. Agri. Res, 27 (3), pp 261-264. 2006.
[46] R.A. Stern, J. Kigel, E. Tomer, S. Gazit, "ÔÇÿMauritius- Lychee Fruit
Development and Reduced Abscission after Treatment with the Auxin
2,4,5-TP,"J. Amer. Sos. Hort. Sci, 120(1), pp 65-70. 1995.
[47] R.A. Stern and S. Gazit, "Reducing fruit drop in lychee with PGR
sprays," In: Basra, A. (Ed.), Plant Growth Regulators in Agriculture and
Horticulture. The Haworth Press Inc., New York, USA, 211- 222 pp.
2000.
[48] R.A. Stern, M. Flaishman, S. Applebaumc, R. Ben-Arie, "Effect of
synthetic auxins on fruit development of ÔÇÿBing- cherry (Prunus avium
L.)," Scientia Horticulturae, 114, pp 275-280. 2007.
[49] G.W. Stutte and J. Gage, "Gibberellins inhibit fruit abscission following
seed abortion in peach," J. Amer. Soc. Hort. Sci, 115, pp 107-110. 1990.
[50] M. Talon, L. Zarcarias, E. Primo-Millo, "Gibberellins and
parthenocarpic ability in developing ovaries of seedless mandarins,"
Plant Physiol, 99, pp 1575-1581. 1992.
[51] D.R. Taylor and J.N. Knight, "Russeting and cracking of apple fruit and
their control with plant growth regulators," Acta Hortic, 2, pp 819-820.
1986.
[52] V. Usenik, D. Kastelec, F. Stampar, "Physicochemical Changes of
Sweet Cherry Fruits Related to Application of Gibberellic Acid," Food
Chem, 90, pp 663-671. 2005.
[53] K. Venkatesan and M.K. Mohideen, "Effect of growth regulators on fruit
characters and yield of pomegranate (Punica granatum L.) cv. Ganesh,"
South Indian Hort, 42 (4), pp 239-244. 1994.
[54] D.G. Voyiatzis and G.P. Paroussi, "The effect of photoperiod and
gibberellic acid on strawberry pollen germination and stamen growth,"
Acta Hortic, 567, pp 257-260. 2002.
[55] D.N. Wang, "The study of the advance flower forcing promotion of
wax-apple. The effect of forcing flower by chemical and culture
treatment of wax-apple," in Chinese J. Agric. Res. China, 32(2), pp129-
138. 1983.
[56] M.N. Westwood, "Temperate-Zone Pomology: Physiology and Culture,"
third ed. Timber Press, Portland, OR, USA 523 pp. 1993.
[57] J.X. Xiao, S. Peng, H.P. Hua, L.H. Jiang, "Effects of calcium nitrate and
IAA on calcium concentration and quality of Satsuma mandarin fruit," J.
Fruit Sci, 22(3), pp 211-215. 2005.
[58] C. Zhang, K. Tanabe, H. Tani, H. Nakajima, M. Mori, A. Itai, E. Sakuno,
"Biologically active gibberellins and abscisic acid in fruit of two latematuring
Japanese pear cultivars with contrasting fruit size," J.