Abstract: Characterization and evaluation of the activity of Vespa basalis DPP-IV, which expressed in Spodoptera frugiperda 21 cells. The expression of rDPP-IV was confirmed by SDS–PAGE, Western blot analyses, LC-MS/MS and measurement of its peptidase specificity. One-step purification by Ni-NTA affinity chromatography and the total amount of rDPP-IV recovered was approximately 6.4mg per liter from infected culture medium; an equivalent amount would be produced by 1x109 infected Sf21 insect cells. Through the affinity purification led to highly stable rDPP-IV enzyme was recovered and with significant peptidase activity. The rDPP-IV exhibited classical Michaelis–Menten kinetics, with kcat/Km in the range of 10-500 mM-1×S-1 for the five synthetic substrates and optimum substrate is Ala-Pro-pNA. As expected in inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor).
Abstract: Different forms of interaction are an integral part of
modern courses. Traditional courses held on-campus might focus on
teacher-student interaction, or student-student interaction, or both.
However when these traditional on-campus courses are to be held as
distance courses there is a risk that these well-designed interactions
will be difficult or impossible to uphold. For example, studentstudent
interaction in traditional project assignments might not work
well if the students are scattered across the world. Thus, even a welldesigned
traditional on-site course cannot without modification be
turned into a distance course. Traditional on-site courses simply have
to be redesigned to become true distance courses. This paper
describes a structured approach which facilitates the redesign of a
traditional course into a distance course. The approach is based on
that the desired forms of course flexibility are identified, and
thereafter that the course activities are redesigned to facilitate
interaction in a distance course. The approach is making use of
known patterns of pedagogic interaction and existing guidelines for
distance education design. The approach is illustrated with an
example course in the field of information systems design.
Abstract: The scientific community has invested a great deal of effort in the fields of discrete wavelet transform in the last few decades. Discrete wavelet transform (DWT) associated with the vector quantization has been proved to be a very useful tool for the compression of image. However, the DWT is very computationally intensive process requiring innovative and computationally efficient method to obtain the image compression. The concurrent transformation of the image can be an important solution to this problem. This paper proposes a model of concurrent DWT for image compression. Additionally, the formal verification of the model has also been performed. Here the Symbolic Model Verifier (SMV) has been used as the formal verification tool. The system has been modeled in SMV and some properties have been verified formally.
Abstract: In the present paper, we propose numerical methods for solving the Stein equation AXC - X - D = 0 where the matrix A is large and sparse. Such problems appear in discrete-time control problems, filtering and image restoration. We consider the case where the matrix D is of full rank and the case where D is factored as a product of two matrices. The proposed methods are Krylov subspace methods based on the block Arnoldi algorithm. We give theoretical results and we report some numerical experiments.
Abstract: In recent past, the Unified Modeling Language (UML) has become the de facto industry standard for object-oriented modeling of the software systems. The syntax and semantics rich UML has encouraged industry to develop several supporting tools including those capable of generating deployable product (code) from the UML models. As a consequence, ensuring the correctness of the model/design has become challenging and extremely important task. In this paper, we present an approach for automatic verification of protocol model/design. As a case study, Session Initiation Protocol (SIP) design is verified for the property, “the CALLER will not converse with the CALLEE before the connection is established between them ". The SIP is modeled using UML statechart diagrams and the desired properties are expressed in temporal logic. Our prototype verifier “UML-SMV" is used to carry out the verification. We subjected an erroneous SIP model to the UML-SMV, the verifier could successfully detect the error (in 76.26ms) and generate the error trace.
Abstract: The study investigates the causal link between trade
openness and economic growth for four South Asian countries for
period 1972-1985 and 1986-2007 to examine the scenario before and
after the implementation of SAARC. Panel cointegration and
FMOLS techniques are employed for short run and long run
estimates. In 1972-85 short run unidirectional causality from GDP to
openness is found whereas, in 1986-2007 there exists bi-directional
causality between GDP and openness. The long run elasticity
magnitude between GDP and openness contains negative sign in
1972-85 which shows that there exists long run negative relationship.
While in time period 1986-2007 the elasticity magnitude has positive
sign that indicates positive causation between GDP and openness. So
it can be concluded that after the implementation of SAARC overall
situation of selected countries got better. Also long run coefficient of
error term suggests that short term equilibrium adjustments are driven
by adjustment back to long run equilibrium.
Abstract: Prior research has examined the relationship between religiosity, religious involvement, and involvement in secular, civic organizations. However, research has not examined the influence of religious involvement on secular, non-civic organizations (i.e. work organizations). This study examines the link between religiosity, religious involvement, and the three-component model of organizational commitment. More specifically, the author hypothesizes that individuals high in religiosity (and religious involvement) will have lower affective, continuance, and normative commitment than less religious (or non-religious) individuals. In addition, it is hypothesized that this relationship is moderated by a third factor: organizational spirituality. Further, the author hypothesizes that for organizations that are spiritual the negative relationship between religiosity and job commitment will be weakened or even negated.
Abstract: Restarted GMRES methods augmented with approximate eigenvectors are widely used for solving large sparse linear systems. Recently a new scheme of augmenting with error approximations is proposed. The main aim of this paper is to develop a restarted GMRES method augmented with the combination of harmonic Ritz vectors and error approximations. We demonstrate that the resulted combination method can gain the advantages of two approaches: (i) effectively deflate the small eigenvalues in magnitude that may hamper the convergence of the method and (ii) partially recover the global optimality lost due to restarting. The effectiveness and efficiency of the new method are demonstrated through various numerical examples.
Abstract: This research attempts to study the feasibility of
augmenting an augmented reality (AR) image card on a Quick
Response (QR) code. The authors have developed a new visual tag,
which contains a QR code and an augmented AR image card. The new
visual tag has features of reading both of the revealed data of the QR
code and the instant data from the AR image card. Furthermore, a
handheld communicating device is used to read and decode the new
visual tag, and then the concealed data of the new visual tag can be
revealed and read through its visual display. In general, the QR code is
designed to store the corresponding data or, as a key, to access the
corresponding data from the server through internet. Those reveled
data from the QR code are represented in text. Normally, the AR
image card is designed to store the corresponding data in
3-Dimensional or animation/video forms. By using QR code's
property of high fault tolerant rate, the new visual tag can access those
two different types of data by using a handheld communicating device.
The new visual tag has an advantage of carrying much more data than
independent QR code or AR image card. The major findings of this
research are: 1) the most efficient area for the designed augmented AR
card augmenting on the QR code is 9% coverage area out of the total
new visual tag-s area, and 2) the best location for the augmented AR
image card augmenting on the QR code is located in the bottom-right
corner of the new visual tag.
Abstract: In this paper a fast motion estimation method for
H.264/AVC named Triplet Search Motion Estimation (TS-ME) is
proposed. Similar to some of the traditional fast motion estimation
methods and their improved proposals which restrict the search points
only to some selected candidates to decrease the computation
complexity, proposed algorithm separate the motion search process to
several steps but with some new features. First, proposed algorithm try
to search the real motion area using proposed triplet patterns instead of
some selected search points to avoid dropping into the local minimum.
Then, in the localized motion area a novel 3-step motion search
algorithm is performed. Proposed search patterns are categorized into
three rings on the basis of the distance from the search center. These
three rings are adaptively selected by referencing the surrounding
motion vectors to early terminate the motion search process. On the
other hand, computation reduction for sub pixel motion search is also
discussed considering the appearance probability of the sub pixel
motion vector. From the simulation results, motion estimation speed
improved by a factor of up to 38 when using proposed algorithm than
that of the reference software of H.264/AVC with ignorable picture
quality loss.
Abstract: Double-diffusive natural convection in an open top
square cavity and heated from the side is studied numerically.
Constant temperatures and concentration are imposed along the right
and left walls while the heat balance at the surface is assumed to obey
Newton-s law of cooling. The finite difference method is used to
solve the dimensionless governing equations. The numerical results
are reported for the effect of Marangoni number, Biot number and
Prandtl number on the contours of streamlines, temperature and
concentration. The predicted results for the average Nusselt number
and Sherwood number are presented for various parametric
conditions. The parameters involved are as follows; the thermal
Marangoni number, 0 ≤ MaT ≤1000 , the solutal Marangoni number,
0 1000 c ≤ Ma ≤ , the Biot number, 0 ≤ Bi ≤ 6 , Grashof number,
5 Gr = 10 and aspect ratio 1. The study focused on both flows; thermal
dominated, N = 0.8 , and compositional dominated, N = 1.3 .
Abstract: Prior research has not effectively investigated how the
profitability of Chinese branches affect FDIs in China [1, 2], so this
study for the first time incorporates realistic earnings information
to systematically investigate effects of innovation, imitation, and
profit factors of FDI diffusions from Taiwan to China. Our nonlinear
least square (NLS) model, which incorporates earnings factors,
forms a nonlinear ordinary differential equation (ODE) in numerical
simulation programs. The model parameters are obtained through
a genetic algorithms (GA) technique and then optimized with the
collected data for the best accuracy. Particularly, Taiwanese regulatory
FDI restrictions are also considered in our modified model to meet
the realistic conditions. To validate the model-s effectiveness, this
investigation compares the prediction accuracy of modified model
with the conventional diffusion model, which does not take account
of the profitability factors.
The results clearly demonstrate the internal influence to be positive,
as early FDI adopters- consistent praises of FDI attract potential firms
to make the same move. The former erects a behavior model for the
latter to imitate their foreign investment decision. Particularly, the
results of modified diffusion models show that the earnings from
Chinese branches are positively related to the internal influence. In
general, the imitating tendency of potential consumers is substantially
hindered by the losses in the Chinese branches, and these firms would
invest less into China. The FDI inflow extension depends on earnings
of Chinese branches, and companies will adjust their FDI strategies
based on the returns. Since this research has proved that earning is
an influential factor on FDI dynamics, our revised model explicitly
performs superior in prediction ability than conventional diffusion
model.
Abstract: This analysis investigates the distortion of flow
measurement and the increase of cavitation along orifice
flowmeter. The analysis using the numerical method (CFD)
validated the distortion of flow measurement through the inlet
velocity profile considering the convergence and grid
dependency. Realizable k-e model was selected and y+ was
about 50 in this numerical analysis. This analysis also estimated
the vulnerability of cavitation effect due to inlet velocity profile.
The investigation concludes that inclined inlet velocity profile
could vary the pressure which was measured at pressure tab
near pipe wall and it led to distort the pressure values ranged
from -3.8% to 5.3% near the orifice plate and to make the
increase of cavitation. The investigation recommends that the
fully developed inlet velocity flow is beneficial to accurate flow
measurement in orifice flowmeter.
Abstract: Carbon nanotubes (CNTs) possess unique structural,
mechanical, thermal and electronic properties, and have been
proposed to be used for applications in many fields. However, to
reach the full potential of the CNTs, many problems still need to be
solved, including the development of an easy and effective
purification procedure, since synthesized CNTs contain impurities,
such as amorphous carbon, carbon nanoparticles and metal particles.
Different purification methods yield different CNT characteristics
and may be suitable for the production of different types of CNTs. In
this study, the effect of different purification chemicals on carbon
nanotube quality was investigated. CNTs were firstly synthesized by
chemical vapor deposition (CVD) of acetylene (C2H2) on a
magnesium oxide (MgO) powder impregnated with an iron nitrate
(Fe(NO3)3·9H2O) solution. The synthesis parameters were selected
as: the synthesis temperature of 800°C, the iron content in the
precursor of 5% and the synthesis time of 30 min. The liquid phase
oxidation method was applied for the purification of the synthesized
CNT materials. Three different acid chemicals (HNO3, H2SO4, and
HCl) were used in the removal of the metal catalysts from the
synthesized CNT material to investigate the possible effects of each
acid solution to the purification step. Purification experiments were
carried out at two different temperatures (75 and 120 °C), two
different acid concentrations (3 and 6 M) and for three different time
intervals (6, 8 and 15 h). A 30% H2O2 : 3M HCl (1:1 v%) solution
was also used in the purification step to remove both the metal
catalysts and the amorphous carbon. The purifications using this
solution were performed at the temperature of 75°C for 8 hours.
Purification efficiencies at different conditions were evaluated by
thermogravimetric analysis. Thermal and electrical properties of
CNTs were also determined. It was found that the obtained electrical
conductivity values for the carbon nanotubes were typical for organic
semiconductor materials and thermal stabilities were changed
depending on the purification chemicals.
Abstract: Understanding of how and where NOx formation
occurs in industrial burner is very important for efficient and clean
operation of utility burners. Also the importance of this problem is
mainly due to its relation to the pollutants produced by more burners
used widely of gas turbine in thermal power plants and glass and steel
industry.
In this article, a numerical model of an industrial burner operating
in MILD combustion is validated with experimental data.. Then
influence of air flow rate and air temperature on combustor
temperature profiles and NOX product are investigated. In order to
modification this study reports on the effects of fuel and air dilution
(with inert gases H2O, CO2, N2), and also influence of lean-premixed
of fuel, on the temperature profiles and NOX emission.
Conservation equations of mass, momentum and energy, and
transport equations of species concentrations, turbulence, combustion
and radiation modeling in addition to NO modeling equations were
solved together to present temperature and NO distribution inside the
burner.
The results shows that dilution, cause to a reduction in value of
temperature and NOX emission, and suppresses any flame
propagation inside the furnace and made the flame inside the furnace
invisible. Dilution with H2O rather than N2 and CO2 decreases further
the value of the NOX. Also with raise of lean-premix level, local
temperature of burner and the value of NOX product are decreases
because of premixing prevents local “hot spots" within the combustor
volume that can lead to significant NOx formation. Also leanpremixing
of fuel with air cause to amount of air in reaction zone is
reach more than amount that supplied as is actually needed to burn
the fuel and this act lead to limiting NOx formation
Abstract: Physiological control of a left ventricle assist device (LVAD) is generally a complicated task due to diverse operating environments and patient variability. In this work, a tracking control algorithm based on sliding mode and feed forward control for a class of discrete-time single input single output (SISO) nonlinear uncertain systems is presented. The controller was developed to track the reference trajectory to a set operating point without inducing suction in the ventricle. The controller regulates the estimated mean pulsatile flow Qp and mean pulsatility index of pump rotational speed PIω that was generated from a model of the assist device. We recall the principle of the sliding mode control theory then we combine the feed-forward control design with the sliding mode control technique to follow the reference trajectory. The uncertainty is replaced by its upper and lower boundary. The controller was tested in a computer simulation covering two scenarios (preload and ventricular contractility). The simulation results prove the effectiveness and the robustness of the proposed controller
Abstract: A new numerical method for solving the twodimensional,
steady, incompressible, viscous flow equations on a
Curvilinear staggered grid is presented in this paper. The proposed
methodology is finite difference based, but essentially takes
advantage of the best features of two well-established numerical
formulations, the finite difference and finite volume methods. Some
weaknesses of the finite difference approach are removed by
exploiting the strengths of the finite volume method. In particular,
the issue of velocity-pressure coupling is dealt with in the proposed
finite difference formulation by developing a pressure correction
equation in a manner similar to the SIMPLE approach commonly
used in finite volume formulations. However, since this is purely a
finite difference formulation, numerical approximation of fluxes is
not required. Results obtained from the present method are based on
the first-order upwind scheme for the convective terms, but the
methodology can easily be modified to accommodate higher order
differencing schemes.
Abstract: In this paper we present a novel technique for data
hiding in binary document images. We use the concept of entropy in
order to identify document specific least distortive areas throughout
the binary document image. The document image is treated as any
other image and the proposed method utilizes the standard document
characteristics for the embedding process. Proposed method
minimizes perceptual distortion due to embedding and allows
watermark extraction without the requirement of any side information
at the decoder end.
Abstract: The problem discussed in this paper involves packing fresh fish fileet of the northern Cod into a standard square container. The fish is first cleaned and split and then collected on a belt ready to be stacked in a container. The aim of our work is to pack the fish into the container with constraints on the amount of overlap allowed for the fileets. The current focus is to design a packing cell that can be real-time and of practical use, while finding the optimal solution to the degree of overlap and minimise the unused space of the container.
Abstract: The numerical simulation of the slip effect via
vicoelastic fluid for 4:1 contraction problem is investigated with
regard to kinematic behaviors of streamlines and stress tensor by
models of the Navier-Stokes and Oldroyd-B equations. Twodimensional
spatial reference system of incompressible creeping flow
with and without slip velocity is determined and the finite element
method of a semi-implicit Taylor-Galerkin pressure-correction is
applied to compute the problem of this Cartesian coordinate system
including the schemes of velocity gradient recovery method and the
streamline-Upwind / Petrov-Galerkin procedure. The slip effect at
channel wall is added to calculate after each time step in order to
intend the alteration of flow path. The result of stress values and the
vortices are reduced by the optimum slip coefficient of 0.1 with near
the outcome of analytical solution.