Enhance Halorespiration in Rhodopseudomonas palustris with Cytochrome P450cam System from Pseudomonas putida

To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.

Experimental and Theoretical Investigation on Notched Specimens Life Under Bending Loading

In this work, bending fatigue life of notched specimens with various notch geometries and dimensions is investigated by experiment and Manson-Caffin theoretical method. In this theoretical method, fatigue life of notched specimens is calculated using the fatigue life obtained from the experiments for plain specimens (without notch). Three notch geometries including ∪-shape, ∨-shape and C -shape notches are considered in this investigation. The experiments are conducted on a rotary bending Moore machine. The specimens are made of a low carbon steel alloy, which has wide application in industry. The stress- life curves are captured for all notched specimen by experiment. The results indicate that Manson-Caffin analytical method cannot adequately predict the fatigue life of notched specimen. However, it seems that the difference between the experiments and Manson-Caffin predictions can be compensated by a proportional factor.

Maximum Norm Analysis of a Nonmatching Grids Method for Nonlinear Elliptic Boundary Value Problem −Δu = f(u)

We provide a maximum norm analysis of a finite element Schwarz alternating method for a nonlinear elliptic boundary value problem of the form -Δu = f(u), on two overlapping sub domains with non matching grids. We consider a domain which is the union of two overlapping sub domains where each sub domain has its own independently generated grid. The two meshes being mutually independent on the overlap region, a triangle belonging to one triangulation does not necessarily belong to the other one. Under a Lipschitz assumption on the nonlinearity, we establish, on each sub domain, an optimal L∞ error estimate between the discrete Schwarz sequence and the exact solution of the boundary value problem.

Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury

Acute kidney injury (AKI) is a new worldwide public health problem. A diagnosis of this disease using creatinine is still a problem in clinical practice. Therefore, a measurement of biomarkers responsible for AKI has received much attention in the past couple years. Cytokine interleukin-18 (IL-18) was reported as one of the early biomarkers for AKI. The most commonly used method to detect this biomarker is an immunoassay. This study used a planar platform to perform an immunoassay using fluorescence for detection. In this study, anti-IL-18 antibody was immobilized onto a microscope slide using a covalent binding method. Make-up samples were diluted at the concentration between 10 to 1000 pg/ml to create a calibration curve. The precision of the system was determined using a coefficient of variability (CV), which was found to be less than 10%. The performance of this immunoassay system was compared with the measurement from ELISA.

Data Acquisition from Cell Phone using Logical Approach

Cell phone forensics to acquire and analyze data in the cellular phone is nowadays being used in a national investigation organization and a private company. In order to collect cellular phone flash memory data, we have two methods. Firstly, it is a logical method which acquires files and directories from the file system of the cell phone flash memory. Secondly, we can get all data from bit-by-bit copy of entire physical memory using a low level access method. In this paper, we describe a forensic tool to acquire cell phone flash memory data using a logical level approach. By our tool, we can get EFS file system and peek memory data with an arbitrary region from Korea CDMA cell phone.

Application of Novel Conserving Immersed Boundary Method to Moving Boundary Problem

A new conserving approach in the context of Immersed Boundary Method (IBM) is presented to simulate one dimensional, incompressible flow in a moving boundary problem. The method employs control volume scheme to simulate the flow field. The concept of ghost node is used at the boundaries to conserve the mass and momentum equations. The Present method implements the conservation laws in all cells including boundary control volumes. Application of the method is studied in a test case with moving boundary. Comparison between the results of this new method and a sharp interface (Image Point Method) IBM algorithm shows a well distinguished improvement in both pressure and velocity fields of the present method. Fluctuations in pressure field are fully resolved in this proposed method. This approach expands the IBM capability to simulate flow field for variety of problems by implementing conservation laws in a fully Cartesian grid compared to other conserving methods.

Using of Positive Psychotheraphy Narratives at School

In this study, how affects the narrative of Positive Psychothreapy which is named “The Three Small Gold Statues "the adloescent-s perception is investigated The sample included 90 adolescents who were high school students. Firstly the narrative was read. Then three questions which were about the narrative were asked. The questions were: What kind of things did you recall what kind of results did you conculde, and also how could you use this narrative in your real life problems. Responds were analyzed by content analysis method. According to research findings the narrative had a great effect for adolescent perceptions, and also the tale could be used at school counselling programs.

Preparation of ATO Conductive Particles with Narrow Size Distribution

Antimosy-doped tin oxide (ATO) particles were prepared via chemical coprecipitation and reverse emulsion. The size and size distribution of ATO particles were obviously decreased via reverse microemulsion method. At the relatively high yield the ATO particles were nearly spherical in shape, meanwhile the crystalline structure and excellent conductivity were reserved, which could satisfy the requirement as composite fillers, such as dielectric filler of polyimide film.

The e-DELPHI Method to Test the Importance Competence and Skills: Case of the Lifelong Learning Spanish Trainers

The lifelong learning is a crucial element in the modernization of European education and training systems. The most important actors in the development process of the lifelong learning are the trainers, whose professional characteristics need new competences and skills in the current labour market. The main objective of this paper is to establish an importance ranking of the new competences, capabilities and skills that the lifelong learning Spanish trainers must possess nowadays. A wide study of secondary sources has allowed the design of a questionnaire that organizes the trainer-s skills and competences. The e-Delphi method is used for realizing a creative, individual and anonymous evaluation by experts on the importance ranking that presents the criteria, sub-criteria and indicators of the e-Delphi questionnaire. Twenty Spanish experts in the lifelong learning have participated in two rounds of the e- DELPHI method. In the first round, the analysis of the experts- evaluation has allowed to establish the ranking of the most importance criteria, sub-criteria and indicators and to eliminate the least valued. The minimum level necessary to reach the consensus among experts has been achieved in the second round.

Particle Simulation of Rarefied Gas Flows witha Superimposed Wall Surface Temperature Gradient in Microgeometries

Rarefied gas flows are often occurred in micro electro mechanical systems and classical CFD could not precisely anticipate the flow and thermal behavior due to the high Knudsen number. Therefore, the heat transfer and the fluid dynamics characteristics of rarefied gas flows in both a two-dimensional simple microchannel and geometry similar to single Knudsen compressor have been investigated with a goal of increasing performance of a actual Knudsen compressor by using a particle simulation method. Thermal transpiration and thermal creep, which are rarefied gas dynamic phenomena, that cause movement of the flow from less to higher temperature is generated by using two different longitude temperature gradients (Linear, Step) along the walls of the flow microchannel. In this study the influence of amount of temperature gradient and governing pressure in various Knudsen numbers and length-to-height ratios have been examined.

Transient Thermal Stresses of Functionally Graded Thick Hollow Cylinder under the Green-Lindsay Model

The transient thermoelastic response of thick hollow cylinder made of functionally graded material under thermal loading is studied. The generalized coupled thermoelasticity based on the Green-Lindsay model is used. The thermal and mechanical properties of the functionally graded material are assumed to be varied in the radial direction according to a power law variation as a function of the volume fractions of the constituents. The thermal and elastic governing equations are solved by using Galerkin finite element method. All the finite element calculations were done by using commercial finite element program FlexPDE. The transient temperature, radial displacement, and thermal stresses distribution through the radial direction of the cylinder are plotted.

Synthesis, Characterization and PL Properties of Cds Nanoparticles Confined within a Functionalized SBA-15 Mesoprous

A simple and dexterous in situ method was introduced to load CdS nanocrystals into organofunctionalized mesoporous, which used an ion-exchange method. The products were extensively characterized by combined spectroscopic methods. X- ray diffraction (XRD) and high-resolution transmission electron microscopy (HRTEM) demonstrated both the maintenance of pore symmetry (space group p6mm) of SBA-15 and the presence of CdS nanocrystals with uniform sizes of about 6 - 8 nm inside the functionalized SBA-15 channels. These mesoporous silica-supported CdS composites showed room temperature photoluminescence properties with a blue shift, indicating the quantum size effect of nanocrystalline CdS.

Design of Stable IIR Digital Filters with Specified Group Delay Errors

The design problem of Infinite Impulse Response (IIR) digital filters is usually expressed as the minimization problem of the complex magnitude error that includes both the magnitude and phase information. However, the group delay of the filter obtained by solving such design problem may be far from the desired group delay. In this paper, we propose a design method of stable IIR digital filters with prespecified maximum group delay errors. In the proposed method, the approximation problems of the magnitude-phase and group delay are separately defined, and these two approximation problems are alternately solved using successive projections. As a result, the proposed method can design the IIR filters that satisfy the prespecified allowable errors for not only the complex magnitude but also the group delay by alternately executing the coefficient update for the magnitude-phase and the group delay approximation. The usefulness of the proposed method is verified through some examples.

Modified Levenberg-Marquardt Method for Neural Networks Training

In this paper a modification on Levenberg-Marquardt algorithm for MLP neural network learning is proposed. The proposed algorithm has good convergence. This method reduces the amount of oscillation in learning procedure. An example is given to show usefulness of this method. Finally a simulation verifies the results of proposed method.

Numerical Solution of Riccati Differential Equations by Using Hybrid Functions and Tau Method

A numerical method for Riccati equation is presented in this work. The method is based on the replacement of unknown functions through a truncated series of hybrid of block-pulse functions and Chebyshev polynomials. The operational matrices of derivative and product of hybrid functions are presented. These matrices together with the tau method are then utilized to transform the differential equation into a system of algebraic equations. Corresponding numerical examples are presented to demonstrate the accuracy of the proposed method.

Prediction of Natural Gas Viscosity using Artificial Neural Network Approach

Prediction of viscosity of natural gas is an important parameter in the energy industries such as natural gas storage and transportation. In this study viscosity of different compositions of natural gas is modeled by using an artificial neural network (ANN) based on back-propagation method. A reliable database including more than 3841 experimental data of viscosity for testing and training of ANN is used. The designed neural network can predict the natural gas viscosity using pseudo-reduced pressure and pseudo-reduced temperature with AARD% of 0.221. The accuracy of designed ANN has been compared to other published empirical models. The comparison indicates that the proposed method can provide accurate results.

An Advanced Stereo Vision Based Obstacle Detection with a Robust Shadow Removal Technique

This paper presents a robust method to detect obstacles in stereo images using shadow removal technique and color information. Stereo vision based obstacle detection is an algorithm that aims to detect and compute obstacle depth using stereo matching and disparity map. The proposed advanced method is divided into three phases, the first phase is detecting obstacles and removing shadows, the second one is matching and the last phase is depth computing. We propose a robust method for detecting obstacles in stereo images using a shadow removal technique based on color information in HIS space, at the first phase. In this paper we use Normalized Cross Correlation (NCC) function matching with a 5 × 5 window and prepare an empty matching table τ and start growing disparity components by drawing a seed s from S which is computed using canny edge detector, and adding it to τ. In this way we achieve higher performance than the previous works [2,17]. A fast stereo matching algorithm is proposed that visits only a small fraction of disparity space in order to find a semi-dense disparity map. It works by growing from a small set of correspondence seeds. The obstacle identified in phase one which appears in the disparity map of phase two enters to the third phase of depth computing. Finally, experimental results are presented to show the effectiveness of the proposed method.

DC Link Floating for Grid Connected PV Converters

Nowadays there are several grid connected converter in the grid system. These grid connected converters are generally the converters of renewable energy sources, industrial four quadrant drives and other converters with DC link. These converters are connected to the grid through a three phase bridge. The standards prescribe the maximal harmonic emission which could be easily limited with high switching frequency. The increased switching losses can be reduced to the half with the utilization of the wellknown Flat-top modulation. The suggested control method is the expansion of the Flat-top modulation with which the losses could be also reduced to the half compared to the Flat-top modulation. Comparing to traditional control these requirements can be simultaneously satisfied much better with the DLF (DC Link Floating) method.

Genetic Combined with a Simplex Algorithm as an Efficient Method for the Detection of a Depressed Ellipsoidal Flaw using the Boundary Element Method

The present work encounters the solution of the defect identification problem with the use of an evolutionary algorithm combined with a simplex method. In more details, a Matlab implementation of Genetic Algorithms is combined with a Simplex method in order to lead to the successful identification of the defect. The influence of the location and the orientation of the depressed ellipsoidal flaw was investigated as well as the use of different amount of static data in the cost function. The results were evaluated according to the ability of the simplex method to locate the global optimum in each test case. In this way, a clear impression regarding the performance of the novel combination of the optimization algorithms, and the influence of the geometrical parameters of the flaw in defect identification problems was obtained.

Mechanical Evaluation of Stainless Steel and Titanium Dynamic Hip Screws for Trochanteric Fracture

This study aimed to present the mechanical performance evaluation of the dynamic hip screw (DHS) for trochanteric fracture by means of finite element method. The analyses were performed based on stainless steel and titanium implant material definitions at various stages of bone healing and including implant removal. The assessment of the mechanical performance used two parameters, von Mises stress to evaluate the strength of bone and implant and elastic strain to evaluate fracture stability. The results show several critical aspects of dynamic hip screw for trochanteric fracture stabilization. In the initial stage of bone healing process, partial weight bearing should be applied to avoid the implant failure. In the late stage of bone healing, stainless steel implant should be removed.