Differentiation between Common Tick Species Using Molecular Biology Techniques in Saudi Arabia

Protein and Esterase electrophoresis were used to genetically identify two Saudi tick species. Engorged females of the camel tick Hyalomma dromedarii (Koch) (Acari: Ixodidae) and the cattle tick Boophilus annulatus (Say) (Acari: Ixodidae) ticks collected from infested camels and cattle in the animals resting house at Hail region in KSA were used. The results showed that there are a variation in both of protein and esterase activity levels and a high polymorphism within and between the genera and species of Hyalomma and Boophilus . In conclusion, the protein and esterase electrophoretic analysis used in the present study could successfully distinguish among tick species, commonly found in Saudi Arabia.

The Effects of Food Deprivation on Hematological Indices and Blood Indicators of Liver Function in Oxyleotris marmorata

Oxyleotris marmorata is considered as undomesticated fish, and its culture occasionally faces a problem of food deprivation. The present study aims to evaluate alteration of hematological indices, blood chemical associated with liver function during 4 weeks of fasting. A non-linear relationships between fasting days and hematological parameters (red blood cell number; y = - 0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818, P>0.05 and mean corpuscular hemoglobin concentration; y = - 0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated. Significant change in hematocrit (Ht) during fasting period was observed. Ht elevated sharply increase at the first weeks of fasting period. Higher Ht also was detected during week 2-4 of fasting time. The significant reduction of hepatosomatic index was observed (y = - 0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase; y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has deleterious effects on hematological indices, liver mass and enzyme associated in liver function. The marked adverse effects occurred after the first week of fasting state.

Simulation and Validation of Spur Gear Heated by Induction using 3d Model

This paper presents the study of hardness profile of spur gear heated by induction heating process in function of the machine parameters, such as the power (kW), the heating time (s) and the generator frequency (kHz). The global work is realized by 3D finite-element simulation applied to the process by coupling and resolving the electromagnetic field and the heat transfer problems, and it was performed in three distinguished steps. First, a Comsol 3D model was built using an adequate formulation and taking into account the material properties and the machine parameters. Second, the convergence study was conducted to optimize the mesh. Then, the surface temperatures and the case depths were deeply analyzed in function of the initial current density and the heating time in medium frequency (MF) and high frequency (HF) heating modes and the edge effect were studied. Finally, the simulations results are validated using experimental tests.

Expression of Leucaena Leucocephala de Wit Chitinase in Transgenic Koshihikari Rice

The cDNA encoding the 326 amino acids of a Class I basic chitinase gene from Leucaena leucocephala de Wit (KB3, Genbank accession: AAM49597) was cloned under the control of CaMV35S promoter in pCAMBIA 1300 and transferred to Koshihikari. Calli of Koshihikari rice was transformed with agrobacterium with this construct expressing the chitinase and β- glucouronidase (GUS). The frequencies of calli 90 % has been obtained from rice seedlings cultured on NB medium. The high regeneration frequencies, 74% was obtained from calli cultured on regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at 25°C. Various factors were studied in order to establish a procedure for the transformation of Koshihikari Agrobacterium tumefaciens. Supplementation of 50 mM acetosyringone to the medium during coculivation was important to enhance the frequency to transient transformation. The 4 week-old scutellum-derived calli were excellent starting materials. Selection medium based on NB medium supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were an optimized medium for selection of transformed rice calli. The percentage of transformation 70 was obtained. Recombinant calli and regenerated rice plants were checked the expression of chitinase and gus by PCR, northern blot gel, southern blot gel, and gus assay. Chitinase and gus were expressed in all parts of recombinant rice. The rice line expressing the KB3 chiitnase was more resistant to the blast fungus Fusarium monoliforme than control line.

Towards Benchmarking English Residential Gas Consumption

The UK Government has emphasized the role of Local Authorities as a key player in its flagship residential energy efficiency strategies, by identifying and targeting areas for energy efficiency improvements. Residential energy consumption in England is characterized by significant geographical variation in energy demand, which makes centralized targeting of areas for energy efficiency intervention difficult. This paper draws on research which aims to understand how demographic, social, economic, urban form and climatic factors influence the geographical variations in English residential gas consumption. The paper reports the findings of a multiple regression model that shows how 64% of the geographical variation in residential gas consumption is accounted for by variations in these factors. Results from this study, after further refinement and validation, can be used by Local Authorities to identify areas within their boundaries that have higher than expected gas consumption, these may be prime targets for energy efficiency initiatives.

Evolutionary Techniques for Model Order Reduction of Large Scale Linear Systems

Recently, genetic algorithms (GA) and particle swarm optimization (PSO) technique have attracted considerable attention among various modern heuristic optimization techniques. The GA has been popular in academia and the industry mainly because of its intuitiveness, ease of implementation, and the ability to effectively solve highly non-linear, mixed integer optimization problems that are typical of complex engineering systems. PSO technique is a relatively recent heuristic search method whose mechanics are inspired by the swarming or collaborative behavior of biological populations. In this paper both PSO and GA optimization are employed for finding stable reduced order models of single-input- single-output large-scale linear systems. Both the techniques guarantee stability of reduced order model if the original high order model is stable. PSO method is based on the minimization of the Integral Squared Error (ISE) between the transient responses of original higher order model and the reduced order model pertaining to a unit step input. Both the methods are illustrated through numerical example from literature and the results are compared with recently published conventional model reduction technique.

Electrical Resistivity of Subsurface: Field and Laboratory Assessment

The objective of this paper is to study the electrical resistivity complexity between field and laboratory measurement, in order to improve the effectiveness of data interpretation for geophysical ground resistivity survey. The geological outcrop in Penang, Malaysia with an obvious layering contact was chosen as the study site. Two dimensional geoelectrical resistivity imaging were used in this study to maps the resistivity distribution of subsurface, whereas few subsurface sample were obtained for laboratory advance. In this study, resistivity of samples in original conditions is measured in laboratory by using time domain low-voltage technique, particularly for granite core sample and soil resistivity measuring set for soil sample. The experimentation results from both schemes are studied, analyzed, calibrated and verified, including basis and correlation, degree of tolerance and characteristics of substance. Consequently, the significant different between both schemes is explained comprehensively within this paper.

Analytical Proposal to Damage Assessment of Buried Continuous Pipelines during External Blast Loading

In this paper, transversal vibration of buried pipelines during loading induced by underground explosions is analyzed. The pipeline is modeled as an infinite beam on an elastic foundation, so that soil-structure interaction is considered by means of transverse linear springs along the pipeline. The pipeline behavior is assumed to be ideal elasto-plastic which an ultimate strain value limits the plastic behavior. The blast loading is considered as a point load, considering the affected length at some point of the pipeline, in which the magnitude decreases exponentially with time. A closed-form solution for the quasi-static problem is carried out for both elastic and elasticperfect plastic behaviors of pipe materials. At the end, a comparative study on steel and polyethylene pipes with different sizes buried in various soil conditions, affected by a predefined underground explosion is conducted, in which effect of each parameter is discussed.

An Overview of Islanding Detection Methods in Photovoltaic Systems

The issue of unintentional islanding in PV grid interconnection still remains as a challenge in grid-connected photovoltaic (PV) systems. This paper discusses the overview of popularly used anti-islanding detection methods, practically applied in PV grid-connected systems. Anti-islanding methods generally can be classified into four major groups, which include passive methods, active methods, hybrid methods and communication base methods. Active methods have been the preferred detection technique over the years due to very small non-detected zone (NDZ) in small scale distribution generation. Passive method is comparatively simpler than active method in terms of circuitry and operations. However, it suffers from large NDZ that significantly reduces its performance. Communication base methods inherit the advantages of active and passive methods with reduced drawbacks. Hybrid method which evolved from the combination of both active and passive methods has been proven to achieve accurate anti-islanding detection by many researchers. For each of the studied anti-islanding methods, the operation analysis is described while the advantages and disadvantages are compared and discussed. It is difficult to pinpoint a generic method for a specific application, because most of the methods discussed are governed by the nature of application and system dependent elements. This study concludes that the setup and operation cost is the vital factor for anti-islanding method selection in order to achieve minimal compromising between cost and system quality.

European Radical Right Parties as Actors in Securitization of Migration

This study reveals that anti-immigrant policies in Europe result from a process of securitization, and that, within this process, radical right parties have been formulating discourses and approaches through a construction process by using some common security themes. These security themes can be classified as national security, economic security, cultural security and internal security. The frequency with which radical right parties use these themes may vary according to the specific historical, social and cultural characteristics of a particular country.

Data Envelopment Analysis with Partially Perfect Objects

This paper presents a simplified version of Data Envelopment Analysis (DEA) - a conventional approach to evaluating the performance and ranking of competitive objects characterized by two groups of factors acting in opposite directions: inputs and outputs. DEA with a Perfect Object (DEA PO) augments the group of actual objects with a virtual Perfect Object - the one having greatest outputs and smallest inputs. It allows for obtaining an explicit analytical solution and making a step to an absolute efficiency. This paper develops this approach further and introduces a DEA model with Partially Perfect Objects. DEA PPO consecutively eliminates the smallest relative inputs or greatest relative outputs, and applies DEA PO to the reduced collections of indicators. The partial efficiency scores are combined to get the weighted efficiency score. The computational scheme remains simple, like that of DEA PO, but the advantage of the DEA PPO is taking into account all of the inputs and outputs for each actual object. Firm evaluation is considered as an example.

A Novel Arabic Text Steganography Method Using Letter Points and Extensions

This paper presents a new steganography approach suitable for Arabic texts. It can be classified under steganography feature coding methods. The approach hides secret information bits within the letters benefiting from their inherited points. To note the specific letters holding secret bits, the scheme considers the two features, the existence of the points in the letters and the redundant Arabic extension character. We use the pointed letters with extension to hold the secret bit 'one' and the un-pointed letters with extension to hold 'zero'. This steganography technique is found attractive to other languages having similar texts to Arabic such as Persian and Urdu.

Modeling of Pulsatile Blood Flow in a Weak Magnetic Field

Blood pulse is an important human physiological signal commonly used for the understanding of the individual physical health. Current methods of non-invasive blood pulse sensing require direct contact or access to the human skin. As such, the performances of these devices tend to vary with time and are subjective to human body fluids (e.g. blood, perspiration and skin-oil) and environmental contaminants (e.g. mud, water, etc). This paper proposes a simulation model for the novel method of non-invasive acquisition of blood pulse using the disturbance created by blood flowing through a localized magnetic field. The simulation model geometry represents a blood vessel, a permanent magnet, a magnetic sensor, surrounding tissues and air in 2-dimensional. In this model, the velocity and pressure fields in the blood stream are described based on Navier-Stroke equations and the walls of the blood vessel are assumed to have no-slip condition. The blood assumes a parabolic profile considering a laminar flow for blood in major artery near the skin. And the inlet velocity follows a sinusoidal equation. This will allow the computational software to compute the interactions between the magnetic vector potential generated by the permanent magnet and the magnetic nanoparticles in the blood. These interactions are simulated based on Maxwell equations at the location where the magnetic sensor is placed. The simulated magnetic field at the sensor location is found to assume similar sinusoidal waveform characteristics as the inlet velocity of the blood. The amplitude of the simulated waveforms at the sensor location are compared with physical measurements on human subjects and found to be highly correlated.

An Evolutionary Statistical Learning Theory

Statistical learning theory was developed by Vapnik. It is a learning theory based on Vapnik-Chervonenkis dimension. It also has been used in learning models as good analytical tools. In general, a learning theory has had several problems. Some of them are local optima and over-fitting problems. As well, statistical learning theory has same problems because the kernel type, kernel parameters, and regularization constant C are determined subjectively by the art of researchers. So, we propose an evolutionary statistical learning theory to settle the problems of original statistical learning theory. Combining evolutionary computing into statistical learning theory, our theory is constructed. We verify improved performances of an evolutionary statistical learning theory using data sets from KDD cup.

X-ray Crystallographic Analysis of MinC N-Terminal Domain from Escherichia coli

MinC plays an important role in bacterial cell division system by inhibiting FtsZ assembly. However, the molecular mechanism of the action is poorly understood. E. coli MinC Nterminus domain was purified and crystallized using 1.4 M sodium citrate pH 6.5 as a precipitant. X-ray diffraction data was collected and processed to 2.3 Å from a native crystal. The crystal belonged to space group P212121, with the unit cell parameters a = 52.7, b = 54.0, c = 64.7 Å. Assuming the presence of two molecules in the asymmetric unit, the Matthews coefficient value is 1.94 Å3 Da-1, which corresponds to a solvent content of 36.5%. The overall structure of MinCN is observed as a dimer form through anti-parallel ß-strand interaction.

Decision Support System for Suppliers

Supplier selection is a multi criteria decision-making process that comprises tangible and intangible factors. The majority of previous supplier selection techniques do not consider strategic perspective. Besides, uncertainty is one of the most important obstacles in supplier selection. For the first, time in this paper, the idea of the algorithm " Knapsack " is used to select suppliers Moreover, an attempt has to be made to take the advantage of a simple numerical method for solving model .This is an innovation to resolve any ambiguity in choosing suppliers. This model has been tried in the suppliers selected in a competitive environment and according to all desired standards of quality and quantity to show the efficiency of the model, an industry sample has been uses.

Combining Fuzzy Logic and Neural Networks in Modeling Landfill Gas Production

Heterogeneity of solid waste characteristics as well as the complex processes taking place within the landfill ecosystem motivated the implementation of soft computing methodologies such as artificial neural networks (ANN), fuzzy logic (FL), and their combination. The present work uses a hybrid ANN-FL model that employs knowledge-based FL to describe the process qualitatively and implements the learning algorithm of ANN to optimize model parameters. The model was developed to simulate and predict the landfill gas production at a given time based on operational parameters. The experimental data used were compiled from lab-scale experiment that involved various operating scenarios. The developed model was validated and statistically analyzed using F-test, linear regression between actual and predicted data, and mean squared error measures. Overall, the simulated landfill gas production rates demonstrated reasonable agreement with actual data. The discussion focused on the effect of the size of training datasets and number of training epochs.

Managing Iterations in Product Design and Development

The inherent iterative nature of product design and development poses significant challenge to reduce the product design and development time (PD). In order to shorten the time to market, organizations have adopted concurrent development where multiple specialized tasks and design activities are carried out in parallel. Iterative nature of work coupled with the overlap of activities can result in unpredictable time to completion and significant rework. Many of the products have missed the time to market window due to unanticipated or rather unplanned iteration and rework. The iterative and often overlapped processes introduce greater amounts of ambiguity in design and development, where the traditional methods and tools of project management provide less value. In this context, identifying critical metrics to understand the iteration probability is an open research area where significant contribution can be made given that iteration has been the key driver of cost and schedule risk in PD projects. Two important questions that the proposed study attempts to address are: Can we predict and identify the number of iterations in a product development flow? Can we provide managerial insights for a better control over iteration? The proposal introduces the concept of decision points and using this concept intends to develop metrics that can provide managerial insights into iteration predictability. By characterizing the product development flow as a network of decision points, the proposed research intends to delve further into iteration probability and attempts to provide more clarity.

Effect of Spray Stand-off on Hardness of Thermally Sprayed Coatings

The mechanical and tribological properties in WC-Co coatings are strongly affected by hardness and elasticity specifications. The results revealed the effect of spraying distance on microhardness and elasticity modulus of coatings. The metallurgical studies have been made on coated samples using optical microscopy, scanning electron microscopy (SEM).

Comparison of Indoor and Outdoor Air Quality in Children Homes at Prenatal Period and One Year Old

Abstract–Indoor air (VOCs) samples were collected simultaneously from variety of indoors (e.g. living rooms, baby-s rooms) and outdoor environments which were voluntarily selected from the houses in which pregnant residents live throughout Ankara. This is the first comprehensive study done in Turkey starting from prenatal period and continued till the babies had one year old. VOCs levels were measured over 76 homes. Air samples were collected in Tenax TA sorbent filled tubes with active sampling method and analyzed with Thermal Desorber and Gas Chromatography/Mass spectrometry (TD-GC/MS). At the first sampling period in the baby-s rooms maximum concentration of toluene was measured about 240.77μg.m-3 and in the living rooms maximum concentration of naphthalene was 180.24μg.m-3. At the second sampling period in the baby-s rooms maximum concentration of toluene was measured about 144.97μg.m-3 and in the living rooms maximum concentration of naphthalene was 247.89μg.m-3. Concentration of TVOCs in the first period was generally higher than the second period.