Production of Milk Clotting Protease by Rhizopus Stolonifer through Optimization of Culture Conditions

The present study describes the biosynthesis of a milkclotting protease by solid state fermentation (SSF) of a locally isolated mould, Rhizopus stolonifer. The production medium was prepared using wheat bran at 50% (w/v). The production conditions are optimized by varying 7 parameters: carbon and nitrogen sources, medium moisture, temperature, pH, fermentation time and inoculum-s size. The maximum enzyme synthesis was measured after 96 h of incubation time at temperature of 28°C. The optimum pH determined was 6 and the inoculum size was 3.106spores/ml. The optimum initial moisture content is comprised between 50 to 70%. The formation of milk clotting protease is enhanced when galactose and peptone are used at 10% (w/v) and 1% (w/v) concentrations respectively. The maximum production of milk clotting protease is 120 US/ml.

In situ Observation of the State and Stability of Hemoglobin Adsorbed onto Glass Surface by Slab Optical Waveguide (SOWG) Spectroscopy

The state and stability of hemoglobin adsorbed on the glass surface was investigated using slab optical waveguide (SOWG) spectroscopy. The peak position of the absorption band of hemoglobin adsorbed on the glass surface was same as that of the hemoglobin in solution. This result suggests that no significant denaturation occurred by adsorption. The adsorption of hemoglobin is relatively strong that the hemoglobin molecules even remained adsorbed after rinsing the cell with buffer solution. The peak shift caused by the reduction of adsorbed hemoglobin was also observed.

Optimized Multiplier Based upon 6-Input Luts and Vedic Mathematics

A new approach has been used for optimized design of multipliers based upon the concepts of Vedic mathematics. The design has been targeted to state-of-the art field-programmable gate arrays (FPGAs). The multiplier generates partial products using Vedic mathematics method by employing basic 4x4 multipliers designed by exploiting 6-input LUTs and multiplexers in the same slices resulting in drastic reduction in area. The multiplier is realized on Xilinx FPGAs using devices Virtex-5 and Virtex-6.Carry Chain Adder was employed to obtain final products. The performance of the proposed multiplier was examined and compared to well-known multipliers such as Booth, Carry Save, Carry ripple, and array multipliers. It is demonstrated that the proposed multiplier is superior in terms of speed as well as power consumption.

Analysis of Genotype Size for an Evolvable Hardware System

The evolution of logic circuits, which falls under the heading of evolvable hardware, is carried out by evolutionary algorithms. These algorithms are able to automatically configure reconfigurable devices. One of main difficulties in developing evolvable hardware with the ability to design functional electrical circuits is to choose the most favourable EA features such as fitness function, chromosome representations, population size, genetic operators and individual selection. Until now several researchers from the evolvable hardware community have used and tuned these parameters and various rules on how to select the value of a particular parameter have been proposed. However, to date, no one has presented a study regarding the size of the chromosome representation (circuit layout) to be used as a platform for the evolution in order to increase the evolvability, reduce the number of generations and optimize the digital logic circuits through reducing the number of logic gates. In this paper this topic has been thoroughly investigated and the optimal parameters for these EA features have been proposed. The evolution of logic circuits has been carried out by an extrinsic evolvable hardware system which uses (1+λ) evolution strategy as the core of the evolution.

Design A Situated Learning Environment Using Mixed Reality Technology - A Case Study

Mixed Reality (MR) is one of the newest technologies explored in education. It promises the potential to promote teaching and learning and making learners- experience more “engaging". However, there still lack of research on designing a virtual learning environment using MR technology. In this paper, we describe the Mixed Reality technology, the characteristics of situated learning as instructional design for virtual environment using mixed reality technology. We also explain a case study that implemented those design and also the system overview.

Effects of Drought on Microbial Activity in Rhizosphere, Soil Hydrophobicity and Leaching of Mineral Nitrogen from Arable Soil Depending on Method of Fertilization

This work presents the first results from the long-term laboratory experiment dealing with impact of drought on soil properties. Three groups of the treatment (A, B and C) with different regime of irrigation were prepared. The soil water content was maintained at 70 % of soil water holding capacity in group A, at 40 % in group B. In group C, soil water regime was maintained in the range of wilting point. Each group of the experiment was divided into three variants (A1 = B1, C1; A2 = B2, C2 etc.) with three repetitions: Variants A1 (B1, C1) were a controls without addition of another fertilizer. Variants A2 (B2, C2) were fertilized with mineral nitrogen fertilizer DAM 390 (0.140 Mg of N per ha) and variants A3 (B3, C3) contained 45 g of Cp per a pot. The significant differences (ANOVA, P

Dynamic Optimization of Industrial Servomechanisms using Motion Laws Based On Bezier Curves

The motion planning procedure described in this paper has been developed in order to eliminate or reduce the residual vibrations of electromechanical positioning systems, without augmenting the motion time (usually imposed by production requirements), nor introducing overtime for vibration damping. The proposed technique is based on a suitable choice of the motion law assigned to the servomotor that drives the mechanism. The reference profile is defined by a Bezier curve, whose shape can be easily changed by modifying some numerical parameters. By means of an optimization technique these parameters can be modified without altering the continuity conditions imposed on the displacement and on its time derivatives at the initial and final time instants.

Optimal Supplementary Damping Controller Design for TCSC Employing RCGA

Optimal supplementary damping controller design for Thyristor Controlled Series Compensator (TCSC) is presented in this paper. For the proposed controller design, a multi-objective fitness function consisting of both damping factors and real part of system electromachanical eigenvalue is used and Real- Coded Genetic Algorithm (RCGA) is employed for the optimal supplementary controller parameters. The performance of the designed supplementary TCSC-based damping controller is tested on a weakly connected power system with different disturbances and loading conditions with parameter variations. Simulation results are presented and compared with a conventional power system stabilizer and also with the TCSC-based supplementary controller when the controller parameters are not optimized to show the effectiveness and robustness of the proposed approach over a wide range of loading conditions and disturbances.

Factors Related to Behaviour Trend of Roasted Coffee Consumers

The purpose of this research was to study the behavior trend factors of consumers to roasted coffee at the petrol station on the route of Rangsit to Nakhon Nayok. The research drew upon data collected from the regular consumers of roasted coffee stands. The majority of respondents was male, 33-39 years old, and holding a bachelor degree. The majority of respondents considered themselves private business proprietors or entrepreneurs and had a monthly income of between 10,000-16,000 baht. The regular coffee consumers spent a minimum coffee expense of between 45 and 300 baht per day. These consumers also displayed good attitude and good motivation which can be ranked as very high. From the hypothesis testing of the behavior trend for the roasted coffee consumers in repurchasing coffee and recommended the coffee to others, the findings revealed that it had a significant correlation. Moreover, the overall attitude towards the marketing mix factors also had a significant correlation with the behavior trend consumers.

Microalbuminuria in Essential Hypertension

Essential hypertension (HTN) usually clusters with other cardiovascular risk factors such as age, overweight, diabetes, insulin resistance and dyslipidemia. The target organ damage (TOD) such as left ventricular hypertrophy, microalbuminuria (MA), acute coronary syndrome (ACS), stroke and cognitive dysfunction takes place early in course of hypertension. Though the prevalence of hypertension is high in India, the relationship between microalbuminuria and target organ damage in hypertension is not well studied. This study aim at detecting MA in essential hypertension and its relation to severity of HTN, duration of HTN, body mass index (BMI), age and TOD such as HTN retinopathy and acute coronary syndrome The present study was done in 100 patients of essential hypertension non diabetics admitted to B.L.D.E.University-s Sri B.M.Patil Medical College, Bijapur, from October 2008 to April 2011. The patients underwent detailed history and clinical examination. Early morning 5 ml of urine sample was collected & MA was estimated by immunoturbidometry method. The relationship of MA with the duration & severity of HTN, BMI, age, sex and TOD's like hypertensive retinopathy, ACS was assessed by univariate analysis. The prevalence of MA in this study was found to be 63 %. In that 42% were male & 21% were female. In this study a significant association between MA and the duration of hypertension (p = 0.036) & (OR =0.438). Longer the duration of hypertension, more possibility of microalbumin in urine. Also there was a significant association between severity of hypertension and MA (p=0.045) and (OR=0.093). MA was positive in 50 (79.4%) patients out of 63, whose blood pressure was >160/100 mm Hg. In this study a significant association between MA and the grades of hypertensive retinopathy (p =0.011) and acute coronary syndrome (p = 0.041) (OR =2.805). Gender and BMI did not pose high risk for MA in this study.The prevalence of MA in essential hypertension is high in this part of the community and MA will increase the risk of developing target organ damage.Early screening of patients with essential hypertension for MA and aggressive management of positive cases might reduce the burden of chronic kidney diseases and cardiovascular diseases in the community.

Studies on Race Car Aerodynamics at Wing in Ground Effect

Numerical studies on race car aerodynamics at wing in ground effect have been carried out using a steady 3d, double precision, pressure-based, and standard k-epsilon turbulence model. Through various parametric analytical studies we have observed that at a particular speed and ground clearance of the wings a favorable negative lift was found high at a particular angle of attack for all the physical models considered in this paper. The fact is that if the ground clearance height to chord length (h/c) is too small, the developing boundary layers from either side (the ground and the lower surface of the wing) can interact, leading to an altered variation of the aerodynamic characteristics at wing in ground effect. Therefore a suitable ground clearance must be predicted throughout the racing for a better performance of the race car, which obviously depends upon the coupled effects of the topography, wing orientation with respect to the ground, the incoming flow features and/or the race car speed. We have concluded that for the design of high performance and high speed race cars the adjustable wings capable to alter the ground clearance and the angles of attack is the best design option for any race car for racing safely with variable speeds.

Simultaneous Segmentation and Recognition of Arabic Characters in an Unconstrained On-Line Cursive Handwritten Document

The last two decades witnessed some advances in the development of an Arabic character recognition (CR) system. Arabic CR faces technical problems not encountered in any other language that make Arabic CR systems achieve relatively low accuracy and retards establishing them as market products. We propose the basic stages towards a system that attacks the problem of recognizing online Arabic cursive handwriting. Rule-based methods are used to perform simultaneous segmentation and recognition of word portions in an unconstrained cursively handwritten document using dynamic programming. The output of these stages is in the form of a ranked list of the possible decisions. A new technique for text line separation is also used.

Improved C-Fuzzy Decision Tree for Intrusion Detection

As the number of networked computers grows, intrusion detection is an essential component in keeping networks secure. Various approaches for intrusion detection are currently being in use with each one has its own merits and demerits. This paper presents our work to test and improve the performance of a new class of decision tree c-fuzzy decision tree to detect intrusion. The work also includes identifying best candidate feature sub set to build the efficient c-fuzzy decision tree based Intrusion Detection System (IDS). We investigated the usefulness of c-fuzzy decision tree for developing IDS with a data partition based on horizontal fragmentation. Empirical results indicate the usefulness of our approach in developing the efficient IDS.

Environmental Friendly Polyurethane Coatings Based On Hyperbranched Resin

Water borne polyurethane (PU) based on newly prepared hyperbranched poly (amine-ester) (HBPAE) was applied and evaluated as organic coating material. HBPAE was prepared through one-pot synthesis between trimethylol propane as a core and AB2 branched monomer which was obtained via Michal addition of methyl methacrylate (MMA) and diethanol amine (DEA). PU was prepared from HBPAE using different ratios of toluene diisocyanate (TDI) to form cured coating film. The prepared HBPAE was characterized using; GPC, FT-IR and 1H-NMR. The mechanical properties (impact, hardness, adhesion, and flexibility), thermal properties (DSC and TGA) and chemical resistance of the applied film were estimated. The results indicated 50% of TDI is the selected ratio. This formulation represents a promising candidate to be used as coating material.

Using the OWA Operator in the Minkowski Distance

We study different types of aggregation operators such as the ordered weighted averaging (OWA) operator and the generalized OWA (GOWA) operator. We analyze the use of OWA operators in the Minkowski distance. We will call these new distance aggregation operator the Minkowski ordered weighted averaging distance (MOWAD) operator. We give a general overview of this type of generalization and study some of their main properties. We also analyze a wide range of particular cases found in this generalization such as the ordered weighted averaging distance (OWAD) operator, the Euclidean ordered weighted averaging distance (EOWAD) operator, the normalized Minkowski distance, etc. Finally, we give an illustrative example of the new approach where we can see the different results obtained by using different aggregation operators.

Direct Numerical Simulation of Subcooled Nucleate Pool Boiling

With the long-term objective of Critical Heat Flux (CHF) prediction, a Direct Numerical Simulation (DNS) framework for simulation of subcooled and saturated nucleate pool boiling is developed. One case of saturated, and three cases of subcooled boiling at different subcooling levels are simulated. Grid refinement study is also reported. Both boiling and condensation phenomena can be computed simultaneously in the proposed numerical framework. Computed bubble detachment diameters of the saturated nucleate pool boiling cases agree well with the experiment. The flow structures around the growing bubble are presented and the accompanying physics is described. The relation between heat flux evolution from the heated wall and the bubble growth is studied, along with investigations of temperature distribution and flow field evolutions.

Experimental Study of Fuel Tank Filling

The refueling of a transparent rectangular fuel tank fitted with a standard filler pipe and roll-over valve was experimentally studied. A fuel-conditioning cart, capable of handling fuels of different Reid vapor pressure at a constant temperature, was used to dispense fuel at the desired rate. The experimental protocol included transient recording of the tank and filler tube pressures while video recording the flow patterns in the filler tube and tank during the refueling process. This information was used to determine the effect of changes in the vent tube diameter, fuel-dispense flow rate and fuel Reid vapor pressure on the pressure-time characteristics and the occurrence of premature fuel filling shut-off and fuel spill-back. Pressure-time curves for the case of normal shut-off demonstrated the classic, three-phase characteristic noted in the literature. The variation of the maximum values of tank dome and filler tube pressures are analyzed in relation to the occurrence of premature shut-off.

A Method under Uncertain Information for the Selection of Students in Interdisciplinary Studies

We present a method for the selection of students in interdisciplinary studies based on the hybrid averaging operator. We assume that the available information given in the problem is uncertain so it is necessary to use interval numbers. Therefore, we suggest a new type of hybrid aggregation called uncertain induced generalized hybrid averaging (UIGHA) operator. It is an aggregation operator that considers the weighted average (WA) and the ordered weighted averaging (OWA) operator in the same formulation. Therefore, we are able to consider the degree of optimism of the decision maker and grades of importance in the same approach. By using interval numbers, we are able to represent the information considering the best and worst possible results so the decision maker gets a more complete view of the decision problem. We develop an illustrative example of the proposed scheme in the selection of students in interdisciplinary studies. We see that with the use of the UIGHA operator we get a more complete representation of the selection problem. Then, the decision maker is able to consider a wide range of alternatives depending on his interests. We also show other potential applications that could be used by using the UIGHA operator in educational problems about selection of different types of resources such as students, professors, etc.

Design and Performance of Adaptive Polarized MIMO MC-SS-CDMA System for Downlink Mobile Communications

In this paper, an adaptive polarized Multiple-Input Multiple-Output (MIMO) Multicarrier Spread Spectrum Code Division Multiple Access (MC-SS-CDMA) system is designed for downlink mobile communications. The proposed system will be examined in Frequency Division Duplex (FDD) mode for both macro urban and suburban environments. For the same transmission bandwidth, a performance comparison between both nonoverlapped and orthogonal Frequency Division Multiplexing (FDM) schemes will be presented. Also, the proposed system will be compared with both the closed loop vertical MIMO MC-SS-CDMA system and the synchronous vertical STBC-MIMO MC-SS-CDMA system. As will be shown, the proposed system introduces a significant performance gain as well as reducing the spatial dimensions of the MIMO system and simplifying the receiver implementation. The effect of the polarization diversity characteristics on the BER performance will be discussed. Also, the impact of excluding the cross-polarization MCSS- CDMA blocks in the base station will be investigated. In addition, the system performance will be evaluated under different Feedback Information (FBI) rates for slowly-varying channels. Finally, a performance comparison for vehicular and pedestrian environments will be presented

A Constrained Clustering Algorithm for the Classification of Industrial Ores

In this paper a Pattern Recognition algorithm based on a constrained version of the k-means clustering algorithm will be presented. The proposed algorithm is a non parametric supervised statistical pattern recognition algorithm, i.e. it works under very mild assumptions on the dataset. The performance of the algorithm will be tested, togheter with a feature extraction technique that captures the information on the closed two-dimensional contour of an image, on images of industrial mineral ores.