Abstract: The state and stability of hemoglobin adsorbed on the
glass surface was investigated using slab optical waveguide (SOWG)
spectroscopy. The peak position of the absorption band of hemoglobin
adsorbed on the glass surface was same as that of the hemoglobin in
solution. This result suggests that no significant denaturation occurred
by adsorption. The adsorption of hemoglobin is relatively strong that
the hemoglobin molecules even remained adsorbed after rinsing the
cell with buffer solution. The peak shift caused by the reduction of
adsorbed hemoglobin was also observed.
Abstract: In situ observation of absorption spectral change of
heptil viologen cation radical (HV+.) was performed by slab optical
waveguide (SOWG) spectroscopy utilizing indium-tin-oxide (ITO)
electrodes. Synchronizing with electrochemical techniques, we
observed the adsorption process of HV+.on the ITO electrode. In this
study, we carried out the ITO-SOWG observations using KBr aqueous
solution containing different concentration of HV to investigate the
concentration dependent spectral change. A few specific absorption
bands, which indicated HV+.existed as both monomer and dimer on
ITO electrode surface with a monolayer or a few layers deposition,
were observed in UV-visible region. The change in the peak position
of the absorption spectra from adsorption species of HV+. were
correlated with the concentration of HV as well as the electrode
potential.