In situ Observation of the State and Stability of Hemoglobin Adsorbed onto Glass Surface by Slab Optical Waveguide (SOWG) Spectroscopy
The state and stability of hemoglobin adsorbed on the
glass surface was investigated using slab optical waveguide (SOWG)
spectroscopy. The peak position of the absorption band of hemoglobin
adsorbed on the glass surface was same as that of the hemoglobin in
solution. This result suggests that no significant denaturation occurred
by adsorption. The adsorption of hemoglobin is relatively strong that
the hemoglobin molecules even remained adsorbed after rinsing the
cell with buffer solution. The peak shift caused by the reduction of
adsorbed hemoglobin was also observed.
[1] K. Ito, A. Fujishima, "An application of optical waveguides to
electrochemistry," in J. Phys. Chem. Vol. 92, 1988, pp. 7043-7045.
[2] K. Kato, A. Takatsu, N. Matsuda, R. Azumi, M. Matsumoto, "A
slab-optical-waveguide absorption spectroscopy of Langmuir-Blodgett
films with a white light excitation source," in Chem. Lett., Vol. 24, 1995,
pp. 437-438.
[3] D. R. Dunphy, S. B. Mendes, S. S. Saavedra, N. R. Armstrong, "The
electroactive integrated optical waveguide: ultrasensitive
spectroelectrochemistry of submonolayer adsorbates," in Anal. Chem.,
Vol. 69, 1997, pp. 3086-3094.
[4] K. Tsunoda, T. Umemura, H. Ueno, E. Okuno, H. Akaiwa, "Adsorption of
methylene blue onto silylated silica surfaces, studied using visible
attenuated total reflection spectroscopy with a slab optical waveguide," in
Appl. Spectrosc. Vol. 57, 2003, 1273-1277.
[5] N. Matsuda, A. Takatsu, K. Kato, "Absorption spectra of rhodamine 6G
by slab optical waveguide spectroscopy," in Chem. Lett., Vol. 25, 1996,
pp. 105-106.
[6] K. Kato, A. Takatsu, N. Matsuda, "UV-visible slab optical waveguide
spectroscopy of cytochrome c adsorbed on a liquid-solid interface," in
Chem. Lett., Vol. 28, 1999, pp. 31-32.
[7] Z.-M. Qi, N. Matsuda, A. Takatsu, K. Kato, "A kinetic study of
cytochrome c adsorption to hydrophilic glass by broad-band,
time-resolved optical waveguide spectroscopy," in J. Phys. Chem. B. Vol.
107, 2003, pp. 6873-6875.
[8] T. Yoshida, H. Asano, J.H. Santos, Z.-M. Qi, A Takatsu, K. Kato, N.
Matsuda, "Studies on adsorption behavior of hemoglobin onto
hydrophobic surface by using slab optical waveguide spectroscopy," in
Electronics and Communications in Japan, vol. J85-C, No. 12, 2003, pp.
1070-1074.
[9] Z.-M. Qi, N. Matsuda, T. Yoshida, H. Asano, A. Takatsu, K. Kato,
"Optical waveguide spectrometer based on thin-film glass plates," in Opt.
Lett. Vol. 27, No. 22, 2002, pp. 2001-2003.
[10] J.H. Santos, N. Matsuda, Z.-M. Qi, T. Yoshida, A. Takatsu, K. Kato,
"Adsorption behavior of cytochrome c, myoglobin and hemoglobin in a
quartz surface probed using slab optical waveguide (SOWG)
spectroscopy ," in Anal. Sci. Vol. 19, 2003, pp. 199-204.
[11] N. Matsuda, J.H. Santos, A. Takatsu, K. Kato, "Spectroelectrochemical
studies on surface immobilized cytochrome c on ITO electrode by slab
optical waveguide spectroscopy," in Thin Solid Films Vol. 438-439, 2003,
pp. 403-406.
[12] K. Fukuda, H. Ohno, "Electron transfer reaction of cytochrome c at the
electrode surface analyzed with non-contact optical waveguide
spectroscopy," in Electroanalysis Vol. 14, 2002, pp. 605-610.
[13] K. Fujita, H. Ohno, "Redox reaction of PEO-modified cytochrome c
adsorbed on the electrode in ion conductive PEO matrix analyzed with
non-contact optical waveguide spectroscopy," in Polym. Adv. Technol.
Vol. 14, 2003, pp. 486-491.
[14] L. Bedouet, H. Adenier, S. Pulvin, C. Bedel-Cloutour, D. Thomas,
"Recovery of the oxidative activity of caged bovine haemoglobin after
UV photolysis," in Biochem. Biophys. Res. Commun. Vol. 320, 2004, pp.
939-944.
[15] X. Liu, L. Shang, Z. Sun, G. Li, "Direct electrochemistry of hemoglobin
in dimethyldioctadecyl ammonium bromide film and its electrocatalysis
to nitric oxide," in J. Biochem. Biophys. Methods, Vol. 62, 2005, pp.
143-151.
[16] R. Deslauriers, D. J. Moffatt, I. C. P. Smith, "Oxygen consumption in
Plasmodium berghei-infected murine red cells: a direct spectrophotomeric
assay in intact erythrocytes," in Biochimica et Biophysica Acta Vol. 886,
1986, pp. 319-326.
[17] C. Causserand Y. Kara, P. Aimar, "Protein fractionation using selective
adsorption on clay surface before filtration," in J. Membr. Sci. Vol. 186,
2001, pp. 165-181.
[18] A.-E. F. Nassar, J. F. Rusling, N. Nakashima, "Electron transfer between
electrodes and heme proteins in protein-DNA films," in J. Am. Chem. Soc.
Vol. 118, 1996, pp. 3043-3044.
[19] Y. Liu, H. Liu, N. Hu "Core-shell nanocluster films of hemoglobin and
clay nanoparticle: direct electrochemistry and electrocatalysis," in
Biophys. Chemist. Vol. 117,, 2005, pp. 27-37.
[20] Z. Dai, S. Liu, H. Ju, H. Chen, "Direct electron transfer and enzymatic
activity of hemoglobin in a hexagonal mesoporous silica matrix," in
Biosens. Bioelectron. Vol. 19, 2004, pp. 861-867.
[21] H.-Y. Gu, A.-M. Yu, H.-Y. Chen, "Direct electron transfer and
characterization of hemoglobin immobilized on a Au
colloid-cysteamine-modified gold electrode," in J. Electroanal. Chem.
Vol. 516, 2001, pp. 119-126.
[22] A. Kondo, S. Oku, F. Murakami, K. Higashitani "Conformational changes
in protein molecules upon adsorption on ultrafine particles," in Colloids
Surf. B: Biointerf. Vol. 1, 1993, pp. 197-201.
[23] A. Kondo, J. Mihara "Comparison of adsorption and conformation of
hemoglobin and myoglobin on various inorganic ultrafine particles," in J.
Colloid Interface Sci. Vol. 177, 1996, pp. 214-221.
[24] B.A. McCool, R. Cashon, G. Karles, W.J. DeSisto, "Silica encapsulated
hemoglobin and myoglobin powders prepared by an aqueous
fast-freezing technique ," in J. Non-Cryst. solids Vol. 333, 2004, pp.
143-149.
[25] J. Chen, J.D. Andrade, R.A. VanWagenen, "Oxy-and deoxyhaemoglobin
adsorption onto glass and polymer surfaces," in Biomaterials Vol. 6,
1985, pp. 231-236.
[26] R. F H. FREEMAN, "Haemoglobin in the digenetic trematode proctoeces
subtenuis (linton)," in Comp. Biochem. Physiol. Vol. 10, 1963, pp.
253-256.
[27] H-Y. Chen, Y-T. Long, "Study of biomolecules by combining
electrochemistry with UV/Vis, IR and surface enhanced Raman scattering
spectroscopy by a novel flow miccrocell," in Anal. Chem. Acta Vol. 382,
1999, pp. 171-177.
[1] K. Ito, A. Fujishima, "An application of optical waveguides to
electrochemistry," in J. Phys. Chem. Vol. 92, 1988, pp. 7043-7045.
[2] K. Kato, A. Takatsu, N. Matsuda, R. Azumi, M. Matsumoto, "A
slab-optical-waveguide absorption spectroscopy of Langmuir-Blodgett
films with a white light excitation source," in Chem. Lett., Vol. 24, 1995,
pp. 437-438.
[3] D. R. Dunphy, S. B. Mendes, S. S. Saavedra, N. R. Armstrong, "The
electroactive integrated optical waveguide: ultrasensitive
spectroelectrochemistry of submonolayer adsorbates," in Anal. Chem.,
Vol. 69, 1997, pp. 3086-3094.
[4] K. Tsunoda, T. Umemura, H. Ueno, E. Okuno, H. Akaiwa, "Adsorption of
methylene blue onto silylated silica surfaces, studied using visible
attenuated total reflection spectroscopy with a slab optical waveguide," in
Appl. Spectrosc. Vol. 57, 2003, 1273-1277.
[5] N. Matsuda, A. Takatsu, K. Kato, "Absorption spectra of rhodamine 6G
by slab optical waveguide spectroscopy," in Chem. Lett., Vol. 25, 1996,
pp. 105-106.
[6] K. Kato, A. Takatsu, N. Matsuda, "UV-visible slab optical waveguide
spectroscopy of cytochrome c adsorbed on a liquid-solid interface," in
Chem. Lett., Vol. 28, 1999, pp. 31-32.
[7] Z.-M. Qi, N. Matsuda, A. Takatsu, K. Kato, "A kinetic study of
cytochrome c adsorption to hydrophilic glass by broad-band,
time-resolved optical waveguide spectroscopy," in J. Phys. Chem. B. Vol.
107, 2003, pp. 6873-6875.
[8] T. Yoshida, H. Asano, J.H. Santos, Z.-M. Qi, A Takatsu, K. Kato, N.
Matsuda, "Studies on adsorption behavior of hemoglobin onto
hydrophobic surface by using slab optical waveguide spectroscopy," in
Electronics and Communications in Japan, vol. J85-C, No. 12, 2003, pp.
1070-1074.
[9] Z.-M. Qi, N. Matsuda, T. Yoshida, H. Asano, A. Takatsu, K. Kato,
"Optical waveguide spectrometer based on thin-film glass plates," in Opt.
Lett. Vol. 27, No. 22, 2002, pp. 2001-2003.
[10] J.H. Santos, N. Matsuda, Z.-M. Qi, T. Yoshida, A. Takatsu, K. Kato,
"Adsorption behavior of cytochrome c, myoglobin and hemoglobin in a
quartz surface probed using slab optical waveguide (SOWG)
spectroscopy ," in Anal. Sci. Vol. 19, 2003, pp. 199-204.
[11] N. Matsuda, J.H. Santos, A. Takatsu, K. Kato, "Spectroelectrochemical
studies on surface immobilized cytochrome c on ITO electrode by slab
optical waveguide spectroscopy," in Thin Solid Films Vol. 438-439, 2003,
pp. 403-406.
[12] K. Fukuda, H. Ohno, "Electron transfer reaction of cytochrome c at the
electrode surface analyzed with non-contact optical waveguide
spectroscopy," in Electroanalysis Vol. 14, 2002, pp. 605-610.
[13] K. Fujita, H. Ohno, "Redox reaction of PEO-modified cytochrome c
adsorbed on the electrode in ion conductive PEO matrix analyzed with
non-contact optical waveguide spectroscopy," in Polym. Adv. Technol.
Vol. 14, 2003, pp. 486-491.
[14] L. Bedouet, H. Adenier, S. Pulvin, C. Bedel-Cloutour, D. Thomas,
"Recovery of the oxidative activity of caged bovine haemoglobin after
UV photolysis," in Biochem. Biophys. Res. Commun. Vol. 320, 2004, pp.
939-944.
[15] X. Liu, L. Shang, Z. Sun, G. Li, "Direct electrochemistry of hemoglobin
in dimethyldioctadecyl ammonium bromide film and its electrocatalysis
to nitric oxide," in J. Biochem. Biophys. Methods, Vol. 62, 2005, pp.
143-151.
[16] R. Deslauriers, D. J. Moffatt, I. C. P. Smith, "Oxygen consumption in
Plasmodium berghei-infected murine red cells: a direct spectrophotomeric
assay in intact erythrocytes," in Biochimica et Biophysica Acta Vol. 886,
1986, pp. 319-326.
[17] C. Causserand Y. Kara, P. Aimar, "Protein fractionation using selective
adsorption on clay surface before filtration," in J. Membr. Sci. Vol. 186,
2001, pp. 165-181.
[18] A.-E. F. Nassar, J. F. Rusling, N. Nakashima, "Electron transfer between
electrodes and heme proteins in protein-DNA films," in J. Am. Chem. Soc.
Vol. 118, 1996, pp. 3043-3044.
[19] Y. Liu, H. Liu, N. Hu "Core-shell nanocluster films of hemoglobin and
clay nanoparticle: direct electrochemistry and electrocatalysis," in
Biophys. Chemist. Vol. 117,, 2005, pp. 27-37.
[20] Z. Dai, S. Liu, H. Ju, H. Chen, "Direct electron transfer and enzymatic
activity of hemoglobin in a hexagonal mesoporous silica matrix," in
Biosens. Bioelectron. Vol. 19, 2004, pp. 861-867.
[21] H.-Y. Gu, A.-M. Yu, H.-Y. Chen, "Direct electron transfer and
characterization of hemoglobin immobilized on a Au
colloid-cysteamine-modified gold electrode," in J. Electroanal. Chem.
Vol. 516, 2001, pp. 119-126.
[22] A. Kondo, S. Oku, F. Murakami, K. Higashitani "Conformational changes
in protein molecules upon adsorption on ultrafine particles," in Colloids
Surf. B: Biointerf. Vol. 1, 1993, pp. 197-201.
[23] A. Kondo, J. Mihara "Comparison of adsorption and conformation of
hemoglobin and myoglobin on various inorganic ultrafine particles," in J.
Colloid Interface Sci. Vol. 177, 1996, pp. 214-221.
[24] B.A. McCool, R. Cashon, G. Karles, W.J. DeSisto, "Silica encapsulated
hemoglobin and myoglobin powders prepared by an aqueous
fast-freezing technique ," in J. Non-Cryst. solids Vol. 333, 2004, pp.
143-149.
[25] J. Chen, J.D. Andrade, R.A. VanWagenen, "Oxy-and deoxyhaemoglobin
adsorption onto glass and polymer surfaces," in Biomaterials Vol. 6,
1985, pp. 231-236.
[26] R. F H. FREEMAN, "Haemoglobin in the digenetic trematode proctoeces
subtenuis (linton)," in Comp. Biochem. Physiol. Vol. 10, 1963, pp.
253-256.
[27] H-Y. Chen, Y-T. Long, "Study of biomolecules by combining
electrochemistry with UV/Vis, IR and surface enhanced Raman scattering
spectroscopy by a novel flow miccrocell," in Anal. Chem. Acta Vol. 382,
1999, pp. 171-177.
@article{"International Journal of Biological, Life and Agricultural Sciences:64168", author = "Masayoshi Matsui and Akiko Nakahara and Akiko Takatsu and Kenji Kato and Naoki Matsuda", title = "In situ Observation of the State and Stability of Hemoglobin Adsorbed onto Glass Surface by Slab Optical Waveguide (SOWG) Spectroscopy", abstract = "The state and stability of hemoglobin adsorbed on the
glass surface was investigated using slab optical waveguide (SOWG)
spectroscopy. The peak position of the absorption band of hemoglobin
adsorbed on the glass surface was same as that of the hemoglobin in
solution. This result suggests that no significant denaturation occurred
by adsorption. The adsorption of hemoglobin is relatively strong that
the hemoglobin molecules even remained adsorbed after rinsing the
cell with buffer solution. The peak shift caused by the reduction of
adsorbed hemoglobin was also observed.", keywords = "hemoglobin, reduction, slab optical waveguide
spectroscopy, solid/liquid interface.", volume = "1", number = "11", pages = "170-4", }
