Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: In order to increase in chickpea quality and
agroecosystem sustainability, field experiments were carried out in
2007 and 2008 growing seasons. In this research the effects of
different organic, chemical and biological fertilizers were
investigated on grain yield and quality of chickpea. Experimental
units were arranged in split-split plots based on randomized complete
blocks with three replications. The highest amounts of yield and yield
components were obtained in G1×N5 interaction. Significant
increasing of N, P, K, Fe and Mg content in leaves and grains
emphasized on superiority of mentioned treatment because each one
of these nutrients has an approved role in chlorophyll synthesis and
photosynthesis ability of the crop. The combined application of
compost, farmyard manure and chemical phosphorus (N5) had the
best grain quality due to high protein, starch and total sugar contents,
low crude fiber and reduced cooking time.
Abstract: The response of growth and yield of rainfed-chickpea
to population density should be evaluated based on long-term
experiments to include the climate variability. This is achievable just
by simulation. In this simulation study, this evaluation was done by
running the CYRUS model for long-term daily weather data of five
locations in Iran. The tested population densities were 7 to 59 (with
interval of 2) stands per square meter. Various functions, including
quadratic, segmented, beta, broken linear, and dent-like functions,
were tested. Considering root mean square of deviations and linear
regression statistics [intercept (a), slope (b), and correlation
coefficient (r)] for predicted versus observed variables, the quadratic
and broken linear functions appeared to be appropriate for describing
the changes in biomass and grain yield, and in harvest index,
respectively. Results indicated that in all locations, grain yield tends
to show increasing trend with crowding the population, but
subsequently decreases. This was also true for biomass in five
locations. The harvest index appeared to have plateau state across
low population densities, but decreasing trend with more increasing
density. The turning point (optimum population density) for grain
yield was 30.68 stands per square meter in Isfahan, 30.54 in Shiraz,
31.47 in Kermanshah, 34.85 in Tabriz, and 32.00 in Mashhad. The
optimum population density for biomass ranged from 24.6 (in
Tabriz) to 35.3 stands per square meter (Mashhad). For harvest index
it varied between 35.87 and 40.12 stands per square meter.
Abstract: The aim of this study was to investigate the effects of
supplementing the diluent of roosters' semen with different levels of
olive oil on motility, viability, morphology and acrosome integrity of
chicken spermatozoa after in vitro storage for up to 72 h. Semen was
collected from 60 White Layer males (62 wk of age) kept in
separated floor pens and randomly divided into six treatment groups
(10 males in each group). Experimental groups were as follows: T1
:fresh semen, T2 : semen extended 1:1 with Al – Daraji 2 diluent
(AD2D) alone, T3 – T6 :semen samples extended 1:1 with AD2D
supplemented with 2 ml, 4 ml, 6 ml or 8 ml of olive oil / 100 ml of
diluent, respectively. Semen samples were then stored at 5 °C for 24
h, 48 h or 72 h. There was a clear influence of diluent
supplementation with olive oil on the spermatozoa motility profile;
olive oil groups (T3, T4, T5 and T6) recorded the highest scores of
mass activity and individual motility during all storage periods
compared to T1 and T2 groups. In addition, the inclusion of olive oil
into semen diluent (T3, T4, T5 and T6) gave significantly higher
percentages of viable spermatozoa, normal morphologically
spermatozoa and intact acrosomes irrespective of storage period.
These results clearly show that supplementation the diluent of
roosters' semen with olive oil can improve semen quality when
semen samples in vitro stored at 5 °C for up to 72 h.
Abstract: Hydrogenated biodiesel is one of the most promising
renewable fuels. It has many advantages over conventional biodiesel,
including higher cetane number, higher heating value, lower
viscosity, and lower corrosiveness due to its absence of oxygen.
From previous work, Pd/TiO2 gave high conversion and selectivity in
hydrogenated biodiesel. In this work, the effect of biomass feedstocks
(i.e. beef fat, chicken fat, pork fat, and jatropha oil) on the production
of hydrogenated biodiesel over Pd/TiO2 has been studied. Biomass
feedstocks were analyzed by ICP-OES (inductively coupled plasma
optical emission spectrometry) to identify the content of impurities
(i.e. P, K, Ca, Na, and Mg). The deoxygenation catalyst, Pd/TiO2,
was prepared by incipient wetness impregnation (IWI) and tested in a
continuous flow packed-bed reactor at 500 psig, 325°C, H2/feed
molar ratio of 30, and LHSV of 4 h-1 for its catalytic activity and
selectivity in hydrodeoxygenation. All feedstocks gave high
selectivity in diesel specification range hydrocarbons and the main
hydrocarbons were n-pentadecane (n-C15) and n-heptadecane (n-
C17), resulting from the decarbonylation/decarboxylation reaction.
Intermediates such as oleic acid, stearic acid, palmitic acid, and esters
were also detected in minor amount. The conversion of triglycerides
in jatropha oil is higher than those of chicken fat, pork fat, and beef
fat, respectively. The higher concentration of metal impurities in
feedstock, the lower conversion of feedstock.
Abstract: The proximate composition, physical traits and
sensory properties of beef and chicken patties incorporated with
various level of dried cornsilk (Maydis stigma) were studied. The
beef and chicken patties were formulated with either 2%, 4% or 6%
of cornsilk. Both cooked beef and chicken patties incorporated with
6% cornsilk recorded the highest protein concentration at 23.3% and
28.42%, respectively. Both cooked beef and chicken patties
containing 6% cornsilk significantly recorded the lowest
concentration of fat at 11.4% and 14.60%, respectively. Beef and
chicken patties formulated with 6% cornsilk recorded the highest
cooking yield at 80.13% and 83.03% compared to other treatments.
The inclusion of cornsilk did not change the sensory properties and
consumer acceptability of cornsilk-based beef and chicken patties.
Cornsilk fibre has been effective in improving cooking yield,
moisture and fat retention of beef and chicken patties
Abstract: An experiment was conducted to determine the effect
of the rearing system on growth performance, carcass yield,
hematological parameters, and feather pecking damage of Thai
indigenous chickens. Three hundred and sixty 1-d-old chicks were
randomly assigned to 2 treatments: indoor treatment and outdoor
access treatment. In the indoor treatment, the chickens were housed
in floor pens (5 birds/m2). In the outdoor access treatment, the
chickens were housed in a similar indoor house; in addition, they also
had an outdoor grass paddock (1 bird/m2). All birds were provided
with same diet and were raised for 16 wk of age. The results showed
that growth performance and carcass yield were not different among
treatment (P>0.05). Outdoor access had no effect on hematological
parameters (P>0.05). However, the feather pecking damage of the
chickens in the outdoor access treatment was lower than that of the
chickens in the indoor treatment (P
Abstract: The main objective of this study was to demonstrate that differentiation of infected and vaccinated animals (DIVA) strategy using different ELISA tests is possible when a subunit vaccine (Haemagglutinin protein) is used to prevent Avian influenza. Special emphasis was placed on the differentiation in the serological response to different components of the AIV (Nucleoprotein, Neuraminidase, Haemagglutinin, Nucleocapsid) between chickens that were vaccinated with a whole virus kill vaccine and recombinant vaccine. Furthermore, the potential use of this DIVA strategy using ELISA assays to detect Neuraminidase 1 (N1) was analyzed as strategy in countries where the field virus is H5N1 and the vaccine used is formulated with H5N2. Detection of AIV-s antibodies to any component in serum was negative for all animals on the study days 0-13. At study day 14 the titers of antibodies against Nucleoprotein (NP) and Nucleocapsid (NC) rose in the experimental groups vaccinated with Volvac® AI KV and were negatives during all the trial in the experimental groups vaccinated with a subunit H5; significant statistically differences were observed between these groups (p < 0.05). The seroconversion either Haemagglutinin or Neuraminidase was evident after 21 days post-vaccination in the experimental groups vaccinated with the respective viral fraction. Regarding the main aim of this study and according with the results that were obtained, use a combination of different ELISA test as a DIVA strategy is feasible when the vaccination is carry out with a subunit H5 vaccine. Also is possible to use the ELISA kit to detect Neuraminidase (either N1 or N2) as a DIVA concept in countries where H5N1 is present and the vaccination programs are done with H5N2 vaccine.
Abstract: Chicken feathers were used as biosorbent for Pb
removal from aqueous solution. In this paper, the kinetics and
equilibrium studies at several pH, temperature, and metal
concentration values are reported. For tested conditions, the Pb
sorption capacity of this poultry waste ranged from 0.8 to 8.3 mg/g.
Optimal conditions for Pb removal by chicken feathers have been
identified. Pseudo-first order and pseudo-second order equations
were used to analyze the experimental data. In addition, the sorption
isotherms were fitted to classical Langmuir and Freundlich models.
Finally, thermodynamic parameters for the sorption process have
been determined. In summary, the results showed that chicken
feathers are an alternative and promising sorbent for the treatment of
effluents polluted by Pb ions.
Abstract: The avian phytohaemagglutinin skin test is being
proved as an in vivo system for the evaluation an avian in vivo T cell
mitogenicity. The test system was one week old Gallus domesticus
broiler Chickens. Five replicates were done for each of the whole,
1:10 dilutions of each of 0.05 IU tuberculin, tetanus immunoglobulin
and DPT vaccine as test materials. The evaluation parameters were
the skin indurations and lymphoblast percentages in bone marrow
lymphocytes.
Tuberculin indurations were 2.06 and 1.26mm for 0.05 IU
respectively while lymphoblast percent were 0.234 and 0.1
accordingly.
The skin indurations of 135mg/ml and 1.35mg/ml tetanus
immunoglobulin were 4.86 and 3.96mm while lymphoblast
percentages were 0.3 and 0.14 respectively.
The whole DPT and 1:10 concentration were with 4.5 and 3.2mm
while their lymphoblast percentages were 0.28 and 0.12 accordingly.
Thus the mitogenicity of the test materials was of dependant type.
Abstract: Clinically, chickens showed progressively developed
clinical signs represented by sever cyanosis of the comb and wattles
with hemorrhage at the shanks, depression, and ruffling feathers with
loss of appetite and high daily mortalities. The ducks showed severe
neurological signs as torticollus, seizures and inability to stand with
mild signs of diarrhea and depression. Grossly, chickens showed
hemorrhages and congestion in most of the organs particularly lung,
liver, spleen, trachea and kidney. The examined ducks showed
multiple petechial hemorrhages, multifocal hemorrhagic necrosis in
the pancreas, pulmonary edema, congestion and hemorrhage in
meninges and congestion in the skeletal muscles. Histopathology
revealed severe congestion and hemorrhages in most of the organs
particularly lung, liver and kidney. Microscopic erosive tracheitis,
sever pulmonary congestion and perivascular oedema and lymphogranulocytic
pneumonia were constant. The liver showed hepatocyts
necrosis and lympho-granulocytic infiltration. The kidney showed
renal tubular necrosis and diffuse congestion. Multifocal, neuronal
necrosis, hemorrhages, multifocal glial nodules, lympho- histiocytic
perivascular cuffing, and occasional neuronophagia were observed in
the cerebrum. Other organs showed moderate changes.
Abstract: This research was conducted to determine responses
of chickpeas to drought in different periods (early period, late period,
no-irrigation, two times irrigation as control). The trial was made in
“Randomized Complete Block Design" with three replications on
2010 and 2011 years in Konya-Turkey. Genotypes were consisted
from 7 lines of ICARDA, 2 certified lines and 1 local population. The
results showed that; as means of years and genotypes, early period
stress showed highest (207.47 kg da-1) seed yield and it was followed
by control (202.33 kg da-1), late period (144.64 kg da-1) and normal
(106.93 kg da-1) stress applications. The genotypes were affected too
much by drought and, the lowest seed was taken from non-irrigated
plots. As the means of years and stress applications, the highest
(196.01 kg da-1) yield was taken from genotype 22255. The reason of
yield variation could be derived from different responses of
genotypes to drought.
Abstract: Sorghum flour was supplemented with 15 and 30%
chickpea flour. Sorghum flour and the supplement were fermented at
35 oC for 0, 8, 16, and 24 h. Changes in pH, titrable acidity, total
soluble solids, protein content, in vitro protein digestibility and
amino acid composition were investigated during fermentation and/or
after supplementation of sorghum flour with chickpea. The pH of the
fermenting material decreased sharply with a concomitant increase in
the titrable acidity. The total soluble solids remained unchanged with
progressive fermentation time. The protein content of sorghum
cultivar was found to be 9.27 and that of chickpea was 22.47%. The
protein content of sorghum cultivar after supplementation with15 and
30% chickpea was significantly (P ≤ 0.05) increased to 11.78 and
14.55%, respectively. The protein digestibility also increased after
fermentation from 13.35 to 30.59 and 40.56% for the supplements,
respectively. Further increment in protein content and digestibility
was observed when supplemented and unsupplemented samples were
fermented for different periods of time. Cooking of fermented
samples was found to increase the protein content slightly and
decreased digestibility for both supplements. Amino acid content of
fermented and fermented and cooked supplements was determined.
Supplementation was found to increase the lysine and therionine
content. Cooking following fermentation decreased lysine,
isoleucine, valine and sulfur containg amino acids.
Abstract: Water is the main component of biological processes.
Water management is important to obtain higher productivity. In this
study, some of the yield components were investigated together with
different drought levels. Four chickpea genotypes (CDC Frontier,
CDC Luna, Sawyer and Sierra) were grown in pots with 3 different
irrigation levels (a dose of 17.5 ml, 35 ml and 70 ml for each pot per
day) after three weeks from sowing. In the research, flowering, pod
set, pod per plant, fertile pod, double seed/pod, stem diameter, plant
weight, seed per plant, 1000 seed weight, seed diameter, vegetation
length and weekly plant height were measured. Consequently,
significant differences were observed on all the investigated
characteristics owing to genotypes (except double seed/pod and stem
diameter), water levels (except first pod, seed weight and height on
3rd week) and genotype x water level interaction (except first pod,
double seed/pod, seed weight and height).
Abstract: Chicken fat was employed as a feedstock for
producing of biodiesel by trasesterification reaction with methanol
and alkali catalyst (KOH). In this study chicken fat biodiesel with
1.4% free fatty acid, methanol and various amount of potassium
hydroxide for 2 hour were studied. The progression of reaction and
conversion of triglycerides to methyl ester were checked by IR
spectrum method.
Abstract: Proximate (moisture, protein, total fat, total ash) and mineral (K, P, Na, Mg, Ca, Zn, Fe, Cu and Mn) composition of chicken giblets (heart, liver and gizzard) were investigated. Phosphorous content, as well as proximate composition, were determined according to recommended ISO methods. The content of all elements, except phosphorus, of the giblets tissues were determined using inductively coupled plasma-optical emission spectrometry (ICP-OES), after dry ashing mineralization. Regarding proximate composition heart was the highest in total fat content, and the lowest in protein content. Liver was the highest in protein and total ash content, while gizzard was the highest in moisture and the lowest in total fat content. Regarding mineral composition liver was the highest for K, P, Ca, Mg, Fe, Zn, Cu, and Mn, while heart was the highest for Na content. The contents of almost all investigated minerals in analysed giblets tissues of chickens from Vojvodina were similar to values reported in the literature, i.e. in national food composition databases of other countries.
Abstract: A feeding trial was conducted to investigate the effect
of periodically use of garlic on performance and carcass
characteristics in broiler chickens. 240 1-day-old Ross broiler chicks
randomly allocated into the 10 dietary treatments (A, B, C, D, E, F,
G, H, I and J) for 6 wk. Treatment A or control group, received basal
diet (based on standards of Ross management guidelines) without
supplementation of garlic powder while B, C and D dietary
treatments were basal diet supplemented with 0.5, 1 and 3% garlic
powder, respectively for the whole time of experiment (6 weeks).
Birds in group E, F and G were fed control diet supplemented with
0.5, 1 and 3% garlic powder, respectively just in their starter diet (0-
21d). Birds in three other treatments (H, I and J) received control diet
for the first 21 days and 0.5, 1 and 3% of garlic powder was added to
their finisher diets, respectively. 1 and 3% supplemented groups in
finisher period had better performance as compared with other
groups. Since present study conducted in optimum and antiseptic
conditions, it seems that better or more responses could be expected
in performance if the raising conditions would not be healthy.
Abstract: Multi-residue analysis method for penicillins was
developed and validated in bovine muscle, chicken, milk, and flatfish.
Detection was based on liquid chromatography tandem mass
spectrometry (LC/MS/MS). The developed method was validated for
specificity, precision, recovery, and linearity. The analytes were
extracted with 80% acetonitrile and clean-up by a single
reversed-phase solid-phase extraction step. Six penicillins presented
recoveries higher than 76% with the exception of Amoxicillin
(59.7%). Relative standard deviations (RSDs) were not more than
10%. LOQs values ranged from 0.1 and to 4.5 ug/kg. The method was
applied to 128 real samples. Benzylpenicillin was detected in 15
samples and Cloxacillin was detected in 7 samples. Oxacillin was
detected in 2 samples. But the detected levels were under the MRL
levels for penicillins in samples.
Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: In order to evaluation the effects of soil organic
matter and biofertilizer on chickpea quality and biological
nitrogen fixation, field experiments were carried out in 2007
and 2008 growing seasons. In this research the effects of
different strategies for soil fertilization were investigated on
grain yield and yield component, minerals, organic compounds
and cooking time of chickpea. Experimental units were
arranged in split-split plots based on randomized complete
blocks with three replications. Main plots consisted of (G1):
establishing a mixed vegetation of Vicia panunica and
Hordeum vulgare and (G2): control, as green manure levels.
Also, five strategies for obtaining the base fertilizer
requirement including (N1): 20 t.ha-1 farmyard manure; (N2):
10 t.ha-1 compost; (N3): 75 kg.ha-1 triple super phosphate;
(N4): 10 t.ha-1 farmyard manure + 5 t.ha-1 compost and (N5):
10 t.ha-1 farmyard manure + 5 t.ha-1 compost + 50 kg.ha-1
triple super phosphate were considered in sub plots.
Furthermoree four levels of biofertilizers consisted of (B1):
Bacillus lentus + Pseudomonas putida; (B2): Trichoderma
harzianum; (B3): Bacillus lentus + Pseudomonas putida +
Trichoderma harzianum; and (B4): control (without
biofertilizers) were arranged in sub-sub plots. Results showed
that integrating biofertilizers (B3) and green manure (G1)
produced the highest grain yield. The highest amounts of yield
were obtained in G1×N5 interaction. Comparison of all 2-way
and 3-way interactions showed that G1N5B3 was determined
as the superior treatment. Significant increasing of N, P2O5,
K2O, Fe and Mg content in leaves and grains emphasized on
superiority of mentioned treatment because each one of these
nutrients has an approved role in chlorophyll synthesis and
photosynthesis abilities of the crops. The combined application
of compost, farmyard manure and chemical phosphorus (N5)
in addition to having the highest yield, had the best grain
quality due to high protein, starch and total sugar contents, low
crude fiber and reduced cooking time.