Abstract: β-Glucosidase is an important enzyme for production
of ethanol from lignocellulose. With hydrolytic activity on
cellooligosaccharides, especially cellobiose, β-glucosidase removes
product inhibitory effect on cellulases and forms fermentable sugars.
In this study, β-glucosidase encoding gene (BGL1) from traditional
starter yeast Saccharomycosis fibuligera BMQ908 was cloned and
expressed in Pichia pastoris. BGL1 of S. fibuligera BMQ 908 shared
98% nucleotide homology with the closest GenBank sequence
(M22475) but identity in amino-acid sequences of catalytic domains.
Recombinant plasmid pPICZαA/BGL1 containing the sequence
encoding BGL1 mature protein and α-factor secretion signal was
constructed and transformed into methylotrophic yeast P. pastoris by
electroporation. The recombinant strain produced single extracellular
protein with molecular weight of 120 kDa and cellobiase activity of
60 IU/ml. The optimum pH of the recombinant β-glucosidase was 5.0
and the optimum temperature was 50°C.
Abstract: The transformation of vocal characteristics aims at
modifying voice such that the intelligibility of aphonic voice is
increased or the voice characteristics of a speaker (source speaker) to
be perceived as if another speaker (target speaker) had uttered it. In
this paper, the current state-of-the-art voice characteristics
transformation methodology is reviewed. Special emphasis is placed
on voice transformation methodology and issues for improving the
transformed speech quality in intelligibility and naturalness are
discussed. In particular, it is suggested to use the modulation theory
of speech as a base for research on high quality voice transformation.
This approach allows one to separate linguistic, expressive, organic
and perspective information of speech, based on an analysis of how
they are fused when speech is produced. Therefore, this theory
provides the fundamentals not only for manipulating non-linguistic,
extra-/paralinguistic and intra-linguistic variables for voice
transformation, but also for paving the way for easily transposing the
existing voice transformation methods to emotion-related voice
quality transformation and speaking style transformation. From the
perspectives of human speech production and perception, the popular
voice transformation techniques are described and classified them
based on the underlying principles either from the speech production
or perception mechanisms or from both. In addition, the advantages
and limitations of voice transformation techniques and the
experimental manipulation of vocal cues are discussed through
examples from past and present research. Finally, a conclusion and
road map are pointed out for more natural voice transformation
algorithms in the future.
Abstract: The main goal of microarray experiments is to quantify the expression of every object on a slide as precisely as possible, with a further goal of clustering the objects. Recently, many studies have discussed clustering issues involving similar patterns of gene expression. This paper presents an application of fuzzy-type methods for clustering DNA microarray data that can be applied to typical comparisons. Clustering and analyses were performed on microarray and simulated data. The results show that fuzzy-possibility c-means clustering substantially improves the findings obtained by others.
Abstract: This study deals with Computational Fluid Dynamics
(CFD) studies of the interactions between the air flow and louvered
fins which equipped the automotive heat exchangers. 3D numerical
simulation results are obtained by using the ANSYS Fluent 13.0 code
and compared to experimental data. The paper studies the effect of
louver angle and louver pitch geometrical parameters, on overall
thermal hydraulic performances of louvered fins.
The comparison between CFD simulations and experimental data
show that established 3-D CFD model gives a good agreement. The
validation agrees, with about 7% of deviation respectively of friction
and Colburn factors to experimental results. As first, it is found that
the louver angle has a strong influence on the heat transfer rate. Then,
louver angle and louver pitch variation of the louvers and their effects
on thermal hydraulic performances are studied. In addition to this
study, it is shown that the second half of the fin takes has a
significant contribution on pressure drop increase without any
increase in heat transfer.
Abstract: In this paper, we present a new and effective image indexing technique that extracts features directly from DCT domain. Our proposed approach is an object-based image indexing. For each block of size 8*8 in DCT domain a feature vector is extracted. Then, feature vectors of all blocks of image using a k-means algorithm is clustered into groups. Each cluster represents a special object of the image. Then we select some clusters that have largest members after clustering. The centroids of the selected clusters are taken as image feature vectors and indexed into the database. Also, we propose an approach for using of proposed image indexing method in automatic image classification. Experimental results on a database of 800 images from 8 semantic groups in automatic image classification are reported.
Abstract: Sharing motivations of viral advertisements by
consumers and the impacts of these advertisements on the
perceptions for brand will be questioned in this study. Three
fundamental questions are answered in the study. These are
advertisement watching and sharing motivations of individuals,
criteria of liking viral advertisement and the impact of individual
attitudes for viral advertisement on brand perception respectively.
This study will be carried out via a viral advertisement which was
practiced in Turkey. The data will be collected by survey method and
the sample of the study consists of individuals who experienced the
practice of sample advertisement. Data will be collected by online
survey method and will be analyzed by using SPSS statistical
package program.
Recently traditional advertisement mind have been changing. New
advertising approaches which have significant impacts on consumers
have been argued. Viral advertising is a modernist advertisement
mind which offers significant advantages to brands apart from
traditional advertising channels such as television, radio and
magazines. Viral advertising also known as Electronic Word-of-
Mouth (eWOM) consists of free spread of convincing messages sent
by brands among interpersonal communication. When compared to
the traditional advertising, a more provocative thematic approach is
argued.
The foundation of this approach is to create advertisements that
are worth sharing with others by consumers. When that fact is taken
into consideration, in a manner of speaking it can also be stated that
viral advertising is media engineering.
The content worth sharing makes people being a volunteer
spokesman of a brand and strengthens the emotional bonds among
brand and consumer. Especially for some sectors in countries which
are having traditional advertising channel limitations, viral
advertising creates vital advantages.
Abstract: Trihalomethanes (THMs) were among the first
disinfection byproducts to be discovered in chlorinated water. The
substances form during a reaction between chlorine and organic
matter in the water. Trihalomethanes are suspected to have negative
effects on birth such as, low birth weight, intrauterine growth
retardation in term births, as well as gestational age and preterm
delivery. There are also some evidences showing these by-products to
be mutagenic and carcinogenic, the greatest amount of evidence being
related to the bladder cancer. However, there exist inconsistencies
regarding such effects of THMs as different studies have provided
different results in this regard. The aim of the present study is to
provide a review of the related researches about the above mentioned
health effects of THMs.
Abstract: This work presents a new phonetic transcription system based on a tree of hierarchical pronunciation rules expressed as context-specific grapheme-phoneme correspondences. The tree is automatically inferred from a phonetic dictionary by incrementally analyzing deeper context levels, eventually representing a minimum set of exhaustive rules that pronounce without errors all the words in the training dictionary and that can be applied to out-of-vocabulary words. The proposed approach improves upon existing rule-tree-based techniques in that it makes use of graphemes, rather than letters, as elementary orthographic units. A new linear algorithm for the segmentation of a word in graphemes is introduced to enable outof- vocabulary grapheme-based phonetic transcription. Exhaustive rule trees provide a canonical representation of the pronunciation rules of a language that can be used not only to pronounce out-of-vocabulary words, but also to analyze and compare the pronunciation rules inferred from different dictionaries. The proposed approach has been implemented in C and tested on Oxford British English and Basic English. Experimental results show that grapheme-based rule trees represent phonetically sound rules and provide better performance than letter-based rule trees.
Abstract: A transient finite element model has been developed
to study the heat transfer and fluid flow during spot Gas Tungsten
Arc Welding (GTAW) on stainless steel. Temperature field, fluid
velocity and electromagnetic fields are computed inside the cathode,
arc-plasma and anode using a unified MHD formulation. The
developed model is then used to study the influence of different
helium-argon gas mixtures on both the energy transferred to the
workpiece and the time evolution of the weld pool dimensions. It is
found that the addition of helium to argon increases the heat flux
density on the weld axis by a factor that can reach 6.5. This induces
an increase in the weld pool depth by a factor of 3. It is also found
that the addition of only 10% of argon to helium decreases
considerably the weld pool depth, which is due to the electrical
conductivity of the mixture that increases significantly when argon is
added to helium.
Abstract: The prevalence of non organic constipation differs
from country to country and the reliability of the estimate rates is
uncertain. Moreover, the clinical relevance of subdividing the
heterogeneous functional constipation disorders into pre-defined
subgroups is largely unknown.. Aim: to estimate the prevalence of
constipation in a population-based sample and determine whether
clinical subgroups can be identified. An age and gender stratified
sample population from 5 Italian cities was evaluated using a
previously validated questionnaire. Data mining by cluster analysis
was used to determine constipation subgroups. Results: 1,500
complete interviews were obtained from 2,083 contacted households
(72%). Self-reported constipation correlated poorly with symptombased
constipation found in 496 subjects (33.1%). Cluster analysis
identified four constipation subgroups which correlated to subgroups
identified according to pre-defined symptom criteria. Significant
differences in socio-demographics and lifestyle were observed
among subgroups.
Abstract: This paper presented a modified efficient inductive
powering link based on ASK modulator and proposed efficient class-
E power amplifier. The design presents the external part which is
located outside the body to transfer power and data to the implanted
devices such as implanted Microsystems to stimulate and monitoring
the nerves and muscles. The system operated with low band
frequency 10MHZ according to industrial- scientific – medical (ISM)
band to avoid the tissue heating. For external part, the modulation
index is 11.1% and the modulation rate 7.2% with data rate 1 Mbit/s
assuming Tbit = 1us. The system has been designed using 0.35-μm
fabricated CMOS technology. The mathematical model is given and
the design is simulated using OrCAD P Spice 16.2 software tool and
for real-time simulation, the electronic workbench MULISIM 11 has
been used.
Abstract: This paper presents a new circuit arrangement for a
current-mode Wheatstone bridge that is suitable for low-voltage
integrated circuits implementation. Compared to the other proposed
circuits, this circuit features severe reduction of the elements number,
low supply voltage (1V) and low power consumption (
Abstract: This paper deals with the helical flow of a Newtonian
fluid in an infinite circular cylinder, due to both longitudinal and
rotational shear stress. The velocity field and the resulting shear
stress are determined by means of the Laplace and finite Hankel
transforms and satisfy all imposed initial and boundary conditions.
For large times, these solutions reduce to the well-known steady-state
solutions.
Abstract: In the present work, we propose a new technique to
enhance the learning capabilities and reduce the computation
intensity of a competitive learning multi-layered neural network
using the K-means clustering algorithm. The proposed model use
multi-layered network architecture with a back propagation learning
mechanism. The K-means algorithm is first applied to the training
dataset to reduce the amount of samples to be presented to the neural
network, by automatically selecting an optimal set of samples. The
obtained results demonstrate that the proposed technique performs
exceptionally in terms of both accuracy and computation time when
applied to the KDD99 dataset compared to a standard learning
schema that use the full dataset.
Abstract: Decrease in hardware costs and advances in computer
networking technologies have led to increased interest in the use of
large-scale parallel and distributed computing systems. One of the
biggest issues in such systems is the development of effective
techniques/algorithms for the distribution of the processes/load of a
parallel program on multiple hosts to achieve goal(s) such as
minimizing execution time, minimizing communication delays,
maximizing resource utilization and maximizing throughput.
Substantive research using queuing analysis and assuming job
arrivals following a Poisson pattern, have shown that in a multi-host
system the probability of one of the hosts being idle while other host
has multiple jobs queued up can be very high. Such imbalances in
system load suggest that performance can be improved by either
transferring jobs from the currently heavily loaded hosts to the lightly
loaded ones or distributing load evenly/fairly among the hosts .The
algorithms known as load balancing algorithms, helps to achieve the
above said goal(s). These algorithms come into two basic categories -
static and dynamic. Whereas static load balancing algorithms (SLB)
take decisions regarding assignment of tasks to processors based on
the average estimated values of process execution times and
communication delays at compile time, Dynamic load balancing
algorithms (DLB) are adaptive to changing situations and take
decisions at run time.
The objective of this paper work is to identify qualitative
parameters for the comparison of above said algorithms. In future this
work can be extended to develop an experimental environment to
study these Load balancing algorithms based on comparative
parameters quantitatively.
Abstract: The element of justice or al-‘adl in the context of
Islamic critical thinking deals with the notion of justice in a thinking
process which critically rationalizes the truth in a fair and objective
manner with no irrelevant interference that can jeopardize a sound
judgment. This Islamic axiological element is vital in technological
decision making as it addresses the issues of religious values and
ethics that are primarily set to fulfill the purpose of human life on
earth. The main objective of this study was to examine and analyze
the perception of Muslim engineering students in Malaysian higher
education institutions towards the concept of al-‘adl as an essential
element of Islamic critical thinking. The study employed mixed
methods approach that comprises data collection from the
questionnaire survey and the interview responses. A total of 557
Muslim engineering undergraduates from six Malaysian universities
participated in the study. The study generally indicated that Muslim
engineering undergraduates in the higher institutions have rather
good comprehension and consciousness for al-‘adl with a slight
awareness on the importance of objective thinking. Nonetheless there
were a few items on the concept that have implied a comparatively
low perception on the rational justice in Islam as the means to grasp
the ultimate truth.
Abstract: Ultrathin (UTD) and Nanoscale (NSD) SOI-MOSFET devices, sharing a similar W/L but with a channel thickness of 46nm and 1.6nm respectively, were fabricated using a selective “gate recessed” process on the same silicon wafer. The electrical transport characterization at room temperature has shown a large difference between the two kinds of devices and has been interpreted in terms of a huge unexpected series resistance. Electrical characteristics of the Nanoscale device, taken in the linear region, can be analytically derived from the ultrathin device ones. A comparison of the structure and composition of the layers, using advanced techniques such as Focused Ion Beam (FIB) and High Resolution TEM (HRTEM) coupled with Energy Dispersive X-ray Spectroscopy (EDS), contributes an explanation as to the difference of transport between the devices.
Abstract: A method and apparatus for noninvasive measurement
of blood glucose concentration based on transilluminated laser beam
via the Index Finger has been reported in this paper. This method
depends on atomic gas (He-Ne) laser operating at 632.8nm
wavelength. During measurement, the index finger is inserted into the
glucose sensing unit, the transilluminated optical signal is converted
into an electrical signal, compared with the reference electrical
signal, and the obtained difference signal is processed by signal
processing unit which presents the results in the form of blood
glucose concentration. This method would enable the monitoring
blood glucose level of the diabetic patient continuously, safely and
noninvasively.
Abstract: In this communication an expression for mean
velocity of waste flow via an open channel is proposed which
is an improvement over Manning formula. The discharges,
storages and depths are computed at all locations of the Lyari river
by utilizing proposed expression. The results attained through
proposed expression are in good agreement with the observed data
and better than those acquired using Manning formula.
Abstract: In this paper, by exploiting a single semiconductor
optical amplifier-Mach Zehnder Interferometer (SOA-MZI), an
integratable all-optical flip-flop (AOFF) is proposed. It is composed
of a SOA-MZI with a bidirectional coupler at the output. Output
signals of both bar and crossbar of the SOA-MZI is fed back to SOAs
located in the arms of the Mach-Zehnder Interferometer (MZI). The
injected photon-rates to the SOAs are modulated by feedback signals
in order to form optical flip-flop. According to numerical analysis,
Gaussian optical pulses with the energy of 15.2 fJ and 20 ps duration
with the full width at half-maximum criterion, can switch the states of
the SR-AOFF. Also simulation results show that the SR-AOFF has
the contrast ratio of 8.5 dB between two states with the transition
time of nearly 20 ps.