Abstract: Background. Food-related allergies and intolerances are frequently occurring in dogs. Diagnosis and monitoring according ‘Golden Standard’ of elimination efficiency is, however, time consuming, expensive, and requires expert clinical setting. In order to facilitate rapid and robust, quantitative testing of intolerance, and determining the individual offending foods, a serological test is implicated for Alimentary Induced Diseases and manifestations. Method. As we developed Medisynx IgG Human Screening Test ELISA before and the dog’ immune system is most similar to humans, we were able to develop Medisynx IgG Dog Screening Test ELISA as well. In this randomized, double-blind, split-sample, retro perspective study 47 dogs suffering from Canine Atopic Dermatitis (CAD) and several secondary induced reactions were included to participate in serological Medisynx IgG Dog Screening Test ELISA (within < 0,02 % SD). Results were expressed as titers relative to the standard OD readings to diagnose alimentary induced diseases and monitoring efficacy of an individual eliminating diet in dogs. Split sample analysis was performed by independently sending 2 times 3 ml serum under two unique codes. Results. The veterinarian monitored these dogs to check dog’ results at least at 3, 7, 21, 49, 70 days and after period of 6 and 12 months on an individual negative diet and a positive challenge (retrospectively) at 6 months. Data of each dog were recorded in a screening form and reported that a complete recovery of all clinical manifestations was observed at or less than 70 days (between 50 and 70 days) in the majority of dogs (44 out of 47 dogs =93.6%). Conclusion. Challenge results showed a significant result of 100% in specificity as well as 100% positive predicted value. On the other hand, sensitivity was 95,7% and negative predictive value was 95,7%. In conclusion, an individual diet based on IgG ELISA in dogs provides a significant improvement of atopic dermatitis and pruritus including all other non-specific defined allergic skin reactions as erythema, itching, biting and gnawing at toes, as well as to several secondary manifestations like chronic diarrhoea, chronic constipation, otitis media, obesity, laziness or inactive behaviour, pain and muscular stiffness causing a movement disorders, excessive lacrimation, hyper behaviour, nervous behaviour and not possible to stay alone at home, anxiety, biting and aggressive behaviour and disobedience behaviour. Furthermore, we conclude that a relatively more severe systemic candidiasis, as shown by relatively higher titer (class 3 and 4 IgG reactions to Candida albicans), influence the duration of recovery from clinical manifestations in affected dogs. These findings are consistent with our preliminary human clinical studies.
Abstract: There is long history of konjac tubers being used as a cure for certain diseases in China and Japan. Konjac flour is prepared from konjac tubers and it contains high concentration of glucomannan. Konjac Glucomannan (KGM) is dietary fiber and the role of which has been demonstrated in weight reduction, lowering blood cholesterol and sugar level, promoting intestinal activity etc. Konjac glucomanan has a property of swelling by absorbing water, more than a hundred times its own weight. Therefore it helps increasing weight of feces, water content of feces, and promotes satiety feeling. Mode of actions of dietary fibre as laxatives agents includes holding water inside the bowel lumen, inhibition of water absorption in the colon and stimulating colonic motility. Number of fecal pellets did not effected in rats were fed on 300 and 600 mg/kg of konjac flour, as well as constipated control and Dulcolax treatment. Water content, weight of fecal pellets and gastrointestinal transit ratio were higher in rats treated with 600 mg/kg than 300 mg/kg of konjac flour. Rats were administered with Dulcolax showed the highest gastrointestinal transit ratio, followed by 600 mg/kg konjac flour. The lowest feed consumption was noted in 600 mg/kg konjac flour diet group.
Abstract: The colonic tissue is a complicated dynamic system
and the colonic activities it generates are composed of irregular
segmental waves, which are referred to as erratic fluctuations or spikes.
They are also highly irregular with subunit fractal structure. The
traditional time-frequency domain statistics like the averaged
amplitude, the motility index and the power spectrum, etc. are
insufficient to describe such fluctuations. Thus the fractal
box-counting dimension is proposed and the fractal scaling behaviors
of the human colonic pressure activities under the physiological
conditions are studied. It is shown that the dimension of the resting
activity is smaller than that of the normal one, whereas the clipped
version, which corresponds to the activity of the constipation patient,
shows with higher fractal dimension. It may indicate a practical
application to assess the colonic motility, which is often indicated by
the colonic pressure activity.
Abstract: The prevalence of non organic constipation differs
from country to country and the reliability of the estimate rates is
uncertain. Moreover, the clinical relevance of subdividing the
heterogeneous functional constipation disorders into pre-defined
subgroups is largely unknown.. Aim: to estimate the prevalence of
constipation in a population-based sample and determine whether
clinical subgroups can be identified. An age and gender stratified
sample population from 5 Italian cities was evaluated using a
previously validated questionnaire. Data mining by cluster analysis
was used to determine constipation subgroups. Results: 1,500
complete interviews were obtained from 2,083 contacted households
(72%). Self-reported constipation correlated poorly with symptombased
constipation found in 496 subjects (33.1%). Cluster analysis
identified four constipation subgroups which correlated to subgroups
identified according to pre-defined symptom criteria. Significant
differences in socio-demographics and lifestyle were observed
among subgroups.