Abstract: Environmental micro-organisms include a large number of taxa and some species that are generally considered nonpathogenic, but can represent a risk in certain conditions, especially for elderly people and immunocompromised individuals. Chemotaxonomic identification techniques are powerful tools for environmental micro-organisms, and cellular fatty acid methyl esters (FAME) content is a powerful fingerprinting identification technique. A system based on an unsupervised artificial neural network (ANN) was set up using the fatty acid profiles of standard bacterial strains, obtained by gas-chromatography, used as learning data. We analysed 45 certified strains belonging to Acinetobacter, Aeromonas, Alcaligenes, Aquaspirillum, Arthrobacter, Bacillus, Brevundimonas, Enterobacter, Flavobacterium, Micrococcus, Pseudomonas, Serratia, Shewanella and Vibrio genera. A set of 79 bacteria isolated from a drinking water line (AMGA, the major water supply system in Genoa) were used as an example for identification compared to standard MIDI method. The resulting ANN output map was found to be a very powerful tool to identify these fresh isolates.
Abstract: The prevalence of non organic constipation differs
from country to country and the reliability of the estimate rates is
uncertain. Moreover, the clinical relevance of subdividing the
heterogeneous functional constipation disorders into pre-defined
subgroups is largely unknown.. Aim: to estimate the prevalence of
constipation in a population-based sample and determine whether
clinical subgroups can be identified. An age and gender stratified
sample population from 5 Italian cities was evaluated using a
previously validated questionnaire. Data mining by cluster analysis
was used to determine constipation subgroups. Results: 1,500
complete interviews were obtained from 2,083 contacted households
(72%). Self-reported constipation correlated poorly with symptombased
constipation found in 496 subjects (33.1%). Cluster analysis
identified four constipation subgroups which correlated to subgroups
identified according to pre-defined symptom criteria. Significant
differences in socio-demographics and lifestyle were observed
among subgroups.