Abstract: To ensure targeting of apoferritin nanocarrier with
encapsulated doxorubicin drug, we used a peptide linker based on a
protein G with N-terminus affinity towards Fc region of antibodies.
To connect the peptide to the surface of apoferritin, the C-terminus of
peptide was made of cysteine with affinity to gold. The surface of
apoferritin with encapsulated doxorubicin (APODOX) was coated
either with gold nanoparticles (APODOX-Nano) or gold(III) chloride
hydrate reduced with sodium borohydride (APODOX-HAu). The
reduction with sodium borohydride caused a loss of doxorubicin
fluorescent properties and probably accompanied with the loss of its
biological activity. Fluorescent properties of APODOX-Nano were
similar to the unmodified APODOX; therefore it was more suited for
the intended use. To evaluate the specificity of apoferritin modified
with antibodies, ELISA-like method was used with the surface of
microtitration plate wells coated by the antigen (goat anti-human IgG
antibodies). To these wells, the nanocarrier was applied. APODOX
without the modification showed 5× lower affinity to the antigen than
APODOX-Nano modified gold and targeting antibodies (human IgG
antibodies).
Abstract: Because blueberries are worldwide recognized as a
good source of beneficial components, their consumption has
increased in the past decades, and so have the scientific works about
their properties. Hence, this work was undertaken to evaluate the
effect of some production and conservation factors on the properties
of blueberries from cultivar Bluecrop. The physical and chemical
analyses were done according to established methodologies and then
all data was treated using software SPSS for assessment of the
possible differences among the factors investigated and/or the
correlations between the variables at study. The results showed that
location of production influenced some of the berries properties
(caliber, sugars, antioxidant activity, color and texture) and that the
age of the bushes was correlated with moisture, sugars and acidity, as
well as lightness. On the other hand, altitude of the farm only was
correlated to sugar content. With regards to conservation, it
influenced only anthocyanins content and DPPH antioxidant activity.
Finally, the type of extract and the order of extraction had a
pronounced influence on all the phenolic properties evaluated.
Abstract: To mitigate the urban heat island effect has become a
global issue when we are faced with the challenge of climate change.
Through literature review, plant photosynthesis can reduce the carbon
dioxide and mitigate the urban heat island effect to a degree. Because
there are not enough open space and parks, green roof has become an
important policy in Taiwan.
We selected elementary school buildings in northern New Taipei
City as research subjects since elementary schools are asked with
priority to build green roof and important educational place to promote
green roof concept. Testo175-H1 recording device was used to record
the temperature and humidity differences between roof surface and
interior space below roof with and without green roof in the long-term.
We also use questionnaires to investigate the awareness of comfort
level of green roof and sensation of teachers and students of the
elementary schools.
The results indicated that the temperature of roof without greening
was higher than that with greening by about 2°C. But sometimes
during noontime, the temperature of green roof was higher than that of
non-green roof probably because of the character of the accumulation
and dissipation of heat of greening. The temperature of the interior
space below green roof was normally lower than that without green
roof by about 1°C, showing that green roof could lower the
temperature. The humidity of the green roof was higher than the one
without greening also indicated that green roof retained water better.
Teachers liked to combine green roof concept in the curriculum,
and students wished all classes can take turns to maintain the green
roof. Teachers and students whose school had integrated green roof
concept in the curriculum were more willing to participate in the
maintenance work of green roof. Teachers and students who may have
access to and touch the green roof can be more aware of the green roof
benefit. We suggest architects to increase the accessibility and
visibility of green roof, such as use it as a part of the activity space.
This idea can be a reference to the green roof curriculum design.
Abstract: Auditory hallucinations among the most invalidating
and distressing experiences reported by patients diagnosed with
schizophrenia, leading to feelings of powerlessness and helplessness
towards their illness. In more severe cases, these auditory
hallucinations can take the form of commanding voices, which are
often related to high suicidality rates in these patients. Several
authors propose that the meanings attributed to the hallucinatory
experience, rather than characteristics like form and content, can be
determinant in patients’ reactions to hallucinatory activity,
particularly in the case of voice-hearing experiences. In this study, 48
patients diagnosed with paranoid schizophrenia presenting auditory
hallucinations were studied. Multiple regression analyses were
computed to study the influence of several developmental aspects,
such as family and social dynamics, bullying, depression, and sociocognitive
variables on the auditory hallucinations, on patients’
attributions and relationships with their voices, and on the resulting
invalidation of hallucinatory experience. Overall, results showed how
relationships with voices can mirror several aspects of interpersonal
relationship with others, and how self-schemas, depression and actual
social relationships help shaping the voice-hearing experience. Early
experiences of victimization and submission help predict the
attributions of omnipotence of the voices, and increased hostility
from parents seems to increase the malevolence of the voices,
suggesting that socio-cognitive factors can significantly contribute to
the etiology and maintenance of auditory hallucinations. The
understanding of the characteristics of auditory hallucinations and the
relationships patients established with their voices can allow the
development of more promising therapeutic interventions that can be
more effective in decreasing invalidation caused by this devastating
mental illness.
Abstract: The article proposed intends to analyze the possibility
(and conditions) of a media regulation law in a democratic rule of law
in the twenty-first century. To do so, will be presented initially the
idea of the public sphere (by Jürgen Habermas), showing how it is
presented as an interface between the citizen and the state (or the
private and public) and how important is it in a deliberative
democracy. Based on this paradigm, the traditional perception of the
role of public information (such as system functional element) and on
the possibility of media regulation will be exposed, due to the public
nature of their activity. A critical argument will then be displayed
from two different perspectives: a) the formal function of the current
media information, considering that the digital age has fragmented
the information access; b) the concept of a constructive democracy,
which reduces the need for representation, changing the strategic
importance of the public sphere. The question to be addressed (based
on the comparative law) is if the regulation is justified in a
polycentric democracy, especially when it operates under the digital
age (with immediate and virtual communication). The proposal is to
be presented in the sense that even in a twenty-first century the media
in a democratic rule of law still has an extremely important role and
may be subject to regulation, but this should be on terms very
different (and narrower) from those usually defended.
Abstract: Purpose: The study aimed to assess the depressant or
antidepressant effects of several Nonsteroidal Anti-Inflammatory
Drugs (NSAIDs) in mice: the selective cyclooxygenase-2 (COX-2)
inhibitor meloxicam, and the non-selective COX-1 and COX-2
inhibitors lornoxicam, sodium metamizole, and ketorolac. The
current literature data regarding such effects of these agents are
scarce.
Materials and methods: The study was carried out on NMRI mice
weighing 20-35 g, kept in a standard laboratory environment. The
study was approved by the Ethics Committee of the University of
Medicine and Pharmacy „Carol Davila”, Bucharest. The study agents
were injected intraperitoneally, 10 mL/kg body weight (bw) 1 hour
before the assessment of the locomotor activity by cage testing (n=10
mice/ group) and 2 hours before the forced swimming tests (n=15).
The study agents were dissolved in normal saline (meloxicam,
sodium metamizole), ethanol 11.8% v/v in normal saline (ketorolac),
or water (lornoxicam), respectively. Negative and positive control
agents were also given (amitryptilline in the forced swimming test).
The cage floor used in the locomotor activity assessment was divided
into 20 equal 10 cm squares. The forced swimming test involved
partial immersion of the mice in cylinders (15/9cm height/diameter)
filled with water (10 cm depth at 28C), where they were left for 6
minutes. The cage endpoint used in the locomotor activity assessment
was the number of treaded squares. Four endpoints were used in the
forced swimming test (immobility latency for the entire 6 minutes,
and immobility, swimming, and climbing scores for the final 4
minutes of the swimming session), recorded by an observer that was
„blinded” to the experimental design. The statistical analysis used the
Levene test for variance homogeneity, ANOVA and post-hoc
analysis as appropriate, Tukey or Tamhane tests.
Results: No statistically significant increase or decrease in the
number of treaded squares was seen in the locomotor activity
assessment of any mice group. In the forced swimming test,
amitryptilline showed an antidepressant effect in each experiment, at
the 10 mg/kg bw dosage. Sodium metamizole was depressant at 100
mg/kg bw (increased the immobility score, p=0.049, Tamhane test),
but not in lower dosages as well (25 and 50 mg/kg bw). Ketorolac
showed an antidepressant effect at the intermediate dosage of 5
mg/kg bw, but not so in the dosages of 2.5 and 10 mg/kg bw,
respectively (increased the swimming score, p=0.012, Tamhane test).
Meloxicam and lornoxicam did not alter the forced swimming
endpoints at any dosage level.
Discussion: 1) Certain NSAIDs caused changes in the forced
swimming patterns without interfering with locomotion. 2) Sodium
metamizole showed a depressant effect, whereas ketorolac proved
antidepressant. Conclusion: NSAID-induced mood changes are not
class effects of these agents and apparently are independent of the
type of inhibited cyclooxygenase (COX-1 or COX-2).
Disclosure: This paper was co-financed from the European Social
Fund, through the Sectorial Operational Programme Human Resources Development 2007-2013, project number POSDRU /159
/1.5 /S /138907 "Excellence in scientific interdisciplinary research,
doctoral and postdoctoral, in the economic, social and medical fields
-EXCELIS", coordinator The Bucharest University of Economic
Studies.
Abstract: Phthalates are ubiquitous environmental pollutants
well known because of their endocrine disrupting activity in human
organism. The aim of our study was, by biological monitoring,
investigate exposure to phthalates of Roma ethnicity group i.e.
children and adults from 5 families (n=29, average age 11.8 ± 7.6
years) living in western Slovakia. Additionally, we analysed some
associations between anthropometric measures, questionnaire data
i.e. socio-economic status, eating and drinking habits, practise of
personal care products and household conditions in comparison with
concentrations of phthalate metabolites. We used for analysis of urine
samples high performance liquid chromatography and tandem mass
spectrometry (HPLC-MS/MS) to determine concentrations of
phthalate metabolites monoethyl phthalate (MEP), mono-n-butyl
phthalate (MnBP), mono-iso-butyl phthalate (MiBP), mono(2-ethyl-
5-hydroxyhexyl) phthalate (5OH-MEHP), mono(2-ethyl-5-oxohexyl)
phthalate (5oxo-MEHP) and mono(2-etylhexyl) phthalate (MEHP).
Our results indicate that ethnicity, lower socioeconomic status and
different housing conditions in Roma population can affect urinary
concentration of phthalate metabolites.
Abstract: Medicinal plants are now gaining attractiveness in
treatment of bacterial infections and food preservation. The objective
of this study was to assess antibacterial activity of some medicinal
plants on pathogenic bacteria. Screening of antibacterial activity of
aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage,
Thyme and Clove against Bacillus cereus, Salmonella typhimurium,
Staphylococcus aureus, Clostridium perfringens and Escherichia coli
were investigated. Antibacterial activity was performed by agar
diffusion and disc diffusion method. Jatropha, Jojoba, Clove and
Ginger extracts showed notable bacterial activity in the first
screening step then selected to be tested against Bacillus cereus
(Gram+), Staphylococcus aureus (Gram+) and Salmonella
typhimurium (Gram−) and their effect was compared using
antibiotics as control. Screening results showed potential antibacterial
activity of the tested plant extracts against the screened bacterial
strains. It was found that methanol extracts exhibited higher
antibacterial activity than aqueous extracts. Methanol extract of
Jatropha showed the highest inhibition zone against Staphylococcus
aureus (Gram+) with 24.00 mm diameter, compared to the other
plant extracts followed by clove. Meanwhile, the inhibition zones of
methanol extracts of Jojoba and Ginger were the same (12mm).The
Gram-positive bacteria were found to be more sensitive to aqueous
and methanol extracts than Gram-negative bacteria.
Abstract: The article represents the results of isolation and
component chromatographic analysis of essential oils of Polygonym
L. plants growing in Kazakhstan in commercial reserves at the
territory of Kazakhstan. The results of research of antiviral activity of
isolated substances to flu virus have been represented in this article.
The main pharmacophore groups in the structure of alkaloids have
been identified.
Abstract: One of the most famous techniques which affect the
efficiency of a production line is the assembly line balancing (ALB)
technique. This paper examines the balancing effect of a whole
production line of a real auto glass manufacturer in three steps. In the
first step, processing time of each activity in the workstations is
generated according to a practical approach. In the second step, the
whole production process is simulated and the bottleneck stations
have been identified, and finally in the third step, several
improvement scenarios are generated to optimize the system
throughput, and the best one is proposed. The main contribution of
the current research is the proposed framework which combines two
famous approaches including Assembly Line Balancing and
Optimization via Simulation technique (OvS). The results show that
the proposed framework could be applied in practical environments,
easily.
Abstract: A simple adaptive voice activity detector (VAD) is
implemented using Gabor and gammatone atomic decomposition of
speech for high Gaussian noise environments. Matching pursuit is
used for atomic decomposition, and is shown to achieve optimal
speech detection capability at high data compression rates for low
signal to noise ratios. The most active dictionary elements found by
matching pursuit are used for the signal reconstruction so that the
algorithm adapts to the individual speakers dominant time-frequency
characteristics. Speech has a high peak to average ratio enabling
matching pursuit greedy heuristic of highest inner products to isolate
high energy speech components in high noise environments. Gabor
and gammatone atoms are both investigated with identical
logarithmically spaced center frequencies, and similar bandwidths.
The algorithm performs equally well for both Gabor and gammatone
atoms with no significant statistical differences. The algorithm
achieves 70% accuracy at a 0 dB SNR, 90% accuracy at a 5 dB SNR
and 98% accuracy at a 20dB SNR using 30d B SNR as a reference
for voice activity.
Abstract: Prosperity of electronic equipment in photocopying
environment not only has improved work efficiency, but also has
changed indoor air quality. Considering the number of photocopying
employed, indoor air quality might be worse than in general office
environments. Determining the contribution from any type of
equipment to indoor air pollution is a complex matter. Non-methane
hydrocarbons are known to have an important role on air quality due
to their high reactivity. The presence of hazardous pollutants in
indoor air has been detected in one photocopying shop in Novi Sad,
Serbia. Air samples were collected and analyzed for five days, during
8-hr working time in three time intervals, whereas three different
sampling points were determined. Using multiple linear regression
model and software package STATISTICA 10 the concentrations of
occupational hazards and microclimates parameters were mutually
correlated. Based on the obtained multiple coefficients of
determination (0.3751, 0.2389 and 0.1975), a weak positive
correlation between the observed variables was determined. Small
values of parameter F indicated that there was no statistically
significant difference between the concentration levels of nonmethane
hydrocarbons and microclimates parameters. The results
showed that variable could be presented by the general regression
model: y = b0 + b1xi1+ b2xi2. Obtained regression equations allow to
measure the quantitative agreement between the variables and thus
obtain more accurate knowledge of their mutual relations.
Abstract: The purpose of this study was to develop a descriptive
profile of the adapted physical activity research using single subject
experimental designs. All research articles using single subject
experimental designs published in the journal of Adapted Physical
Activity Quarterly from 1984 to 2013 were employed as the data
source. Each of the articles was coded in a subcategory of seven
categories: (a) the size of sample; (b) the age of participants; (c) the
type of disabilities; (d) the type of data analysis; (e) the type of
designs, (f) the independent variable, and (g) the dependent variable.
Frequencies, percentages, and trend inspection were used to analyze
the data and develop a profile. The profile developed characterizes a
small portion of research articles used single subject designs, in
which most researchers used a small sample size, recruited children
as subjects, emphasized learning and behavior impairments, selected
visual inspection with descriptive statistics, preferred a multiple
baseline design, focused on effects of therapy, inclusion, and
strategy, and measured desired behaviors more often, with a
decreasing trend over years.
Abstract: The article represents the results of clinical researches
of composite herbal medicinal product based on essential oils of
Sedum plants growing in Kazakhstan in commercial reserves at the
territory of Kazakhstan. The results of comparative analysis are
represented in obstetric-gynecologic practice during combined
therapy for postnatal complications, inflammatory infiltrates in the
area of surgical wounds including wounds after caesarean section.
Abstract: The article represents the results of research of
antitumor activity of different structural types of plant flavonoids
extracted by authors from Polygonum L. plants in commercial
reserves at the territory of the Republic of Kazakhstan. For the first
time ever the results comparative research of antitumor activity of
plant flavonoids of different structural groups and their synthetic
derivatives have been represented. The results of determination of
toxicity of flavonoids in single parenteral infusion conditions have
been represented. Experimental substantiation of possible
mechanisms of antiproliferative and cytotoxic action of flavonoids
has been suggested. The perspectives of usage of plant flavonoids as
medications and creation of effective dosage forms of antitumor
medicines on their basis have been substantiated.
Abstract: This work assesses the cortical and the sub-cortical
neural activity recorded from rodents using entropy and mutual
information based approaches to study how hypothermia affects neural
activity. By applying the multi-scale entropy and Shannon entropy, we
quantify the degree of the regularity embedded in the cortical and
sub-cortical neurons and characterize the dependency of entropy of
these regions on temperature. We study also the degree of the mutual
information on thalamocortical pathway depending on temperature.
The latter is most likely an indicator of coupling between these highly
connected structures in response to temperature manipulation leading
to arousal after global cerebral ischemia.
Abstract: Pt/γ-Al2O3 membrane catalysts were prepared via an
evaporative-crystallization deposition method. The obtained Pt/γ-
Al2O3 catalyst activity was tested after characterization (SEM-EDAX
observation, BET measurement, permeability assessment) in the
catalytic oxidation of selected volatile organic compound (VOC) i.e.
propane, fed in mixture of oxygen. The VOC conversion (nearly
90%) obtained by varying the operating temperature showed that
flow-through membrane reactor might do better in the abatement of
VOCs.
Abstract: This work is the first dowel in a rather wide research
activity in collaboration with Euro Mediterranean Center for Climate
Changes, aimed at introducing scalable approaches in Ocean
Circulation Models. We discuss designing and implementation of
a parallel algorithm for solving the Variational Data Assimilation
(DA) problem on Graphics Processing Units (GPUs). The algorithm
is based on the fully scalable 3DVar DA model, previously proposed
by the authors, which uses a Domain Decomposition approach
(we refer to this model as the DD-DA model). We proceed with
an incremental porting process consisting of 3 distinct stages:
requirements and source code analysis, incremental development of
CUDA kernels, testing and optimization. Experiments confirm the
theoretic performance analysis based on the so-called scale up factor
demonstrating that the DD-DA model can be suitably mapped on
GPU architectures.
Abstract: The potential neuroprotective effect of Phyllantus
nuriri against Fe2+ and sodium nitroprusside (SNP) induced oxidative
stress in mitochondria of rats brain was evaluated. Cellular viability
was assessed by MTT reduction, reactive oxygen species (ROS)
generation was measured using the probe 2,7-dichlorofluoresce
indiacetate (DCFH-DA). Glutathione content was measured using
dithionitrobenzoic acid (DTNB). Fe2+ (10μM) and SNP (5μM)
significantly decreased mitochondrial activity, assessed by MTT
reduction assay, in a dose-dependent manner, this occurred in parallel
with increased glutathione oxidation, ROS production and lipid
peroxidation end-products (thiobarbituric acid reactive substances,
TBARS). The co-incubation with methanolic extract of Phyllantus
nuriri (10-200 μg/ml) reduced the disruption of mitochondrial
activity, gluthathione oxidation, ROS production as well as the
increase in TBARS levels caused by both Fe2+ and SNP in a dose
dependent manner. HPLC analysis of the extract revealed the
presence of gallic acid (20.540.01), caffeic acid (7.930.02), rutin
(25.310.05), quercetin (31.280.03) and kaemferol (14.360.01).
This result suggests that these phytochemicals account for the
protective actions of P. niruri against Fe2+ and SNP -induced
oxidative stress. Our results show that P. nuriri consist important
bioactive molecules in the search for an improved therapy against the
deleterious effects of Fe2+, an intrinsic producer of reactive oxygen
species (ROS), that leads to neuronal oxidative stress and
neurodegeneration.
Abstract: The effect of N2 pretreatment on the catalytic activity
of tungsten-based catalysts was investigated in the metathesis of
ethylene and trans-2-butene at 450oC and atmospheric pressure. The
presence of tungsten active species was confirmed by UV-Vis and
Raman spectroscopy. Compared to the WO3-based catalysts treated
in air, higher amount of WO4 2-tetrahedral species and lower amount
of WO3 crystalline species were observed on the N2-treated ones.
These contribute to the higher conversion of 2-butene and propylene
selectivity during 10 h time-on-stream. Moreover, N2 treatment led to
lower amount of coke formation as revealed by TPO of the spent
catalysts.