Abstract: Mammalian genomes contain large number of
retroelements (SINEs, LINEs and LTRs) which could affect
expression of protein coding genes through associated transcription
factor binding sites (TFBS). Activity of the retroelement-associated
TFBS in many genes is confirmed experimentally but their global
functional impact remains unclear. Human SINEs (Alu repeats) and
mouse SINEs (B1 and B2 repeats) are known to be clustered in GCrich
gene rich genome segments consistent with the view that they
can contribute to regulation of gene expression. We have shown
earlier that Alu are involved in formation of cis-regulatory modules
(clusters of TFBS) in human promoters, and other authors reported
that Alu located near promoter CpG islands have an increased
frequency of CpG dinucleotides suggesting that these Alu are
undermethylated. Human Alu and mouse B1/B2 elements have an
internal bipartite promoter for RNA polymerase III containing
conserved sequence motif called B-box which can bind basal
transcription complex TFIIIC. It has been recently shown that TFIIIC
binding to B-box leads to formation of a boundary which limits
spread of repressive chromatin modifications in S. pombe. SINEassociated
B-boxes may have similar function but conservation of
TFIIIC binding sites in SINEs located near mammalian promoters
has not been studied earlier. Here we analysed abundance and
distribution of retroelements (SINEs, LINEs and LTRs) in annotated
sequences of the Database of mammalian transcription start sites
(DBTSS). Fractions of SINEs in human and mouse promoters are
slightly lower than in all genome but >40% of human and mouse
promoters contain Alu or B1/B2 elements within -1000 to +200 bp
interval relative to transcription start site (TSS). Most of these SINEs
is associated with distal segments of promoters (-1000 to -200 bp
relative to TSS) indicating that their insertion at distances >200 bp
upstream of TSS is tolerated during evolution. Distribution of SINEs
in promoters correlates negatively with the distribution of CpG
sequences. Using analysis of abundance of 12-mer motifs from the
B1 and Alu consensus sequences in genome and DBTSS it has been
confirmed that some subsegments of Alu and B1 elements are poorly
conserved which depends in part on the presence of CpG
dinucleotides. One of these CpG-containing subsegments in B1
elements overlaps with SINE-associated B-box and it shows better
conservation in DBTSS compared to genomic sequences. It has been
also studied conservation in DBTSS and genome of the B-box
containing segments of old (AluJ, AluS) and young (AluY) Alu
repeats and found that CpG sequence of the B-box of old Alu is
better conserved in DBTSS than in genome. This indicates that Bbox-
associated CpGs in promoters are better protected from
methylation and mutation than B-box-associated CpGs in genomic
SINEs. These results are consistent with the view that potential
TFIIIC binding motifs in SINEs associated with human and mouse
promoters may be functionally important. These motifs may protect
promoters from repressive histone modifications which spread from
adjacent sequences. This can potentially explain well known
clustering of SINEs in GC-rich gene rich genome compartments and
existence of unmethylated CpG islands.
Abstract: This paper regards the phenomena of intensive suburbanization and urbanization in Olomouc city and in Olomouc region in general for the period of 1986–2009. A Remote Sensing approach that involves tracking of changes in Land Cover units is proposed to quantify the urbanization state and trends in temporal and spatial aspects. It actually consisted of two approaches, Experiment 1 and Experiment 2 which implied two different image classification solutions in order to provide Land Cover maps for each 1986–2009 time split available in the Landsat image set. Experiment 1 dealt with the unsupervised classification, while Experiment 2 involved semi- supervised classification, using a combination of object-based and pixel-based classifiers. The resulting Land Cover maps were subsequently quantified for the proportion of urban area unit and its trend through time, and also for the urban area unit stability, yielding the relation of spatial and temporal development of the urban area unit. Some outcomes seem promising but there is indisputably room for improvements of source data and also processing and filtering.
Abstract: Diesel Engines emit complex mixtures of inorganic
and organic compounds in the form of both solid and vapour phase
particles. Most of the particulates released are ultrafine nanoparticles
which are detrimental to human health and can easily enter the body
by respiration. The emissions standards on particulate matter release
from diesel engines are constantly upgraded within the European
Union and with future regulations based on the particles numbers
released instead of merely mass, the need for effective aftertreatment
devices will increase. Standard particulate filters in the form of wall
flow filters can have problems with high soot accumulation,
producing a large exhaust backpressure. A potential solution would
be to combine the standard filter with a flow through filter to reduce
the load on the wall flow filter. In this paper soot particle trapping has
been simulated in different continuous flow filters of monolithic
structure including the use of promoters, at laminar flow conditions.
An Euler Lagrange model, the discrete phase model in Ansys used
with user defined functions for forces acting on particles. A method
to quickly screen trapping of 5 nm and 10 nm particles in different
catalysts designs with tracers was also developed.
Simulations of square duct monoliths with promoters show that the
strength of the vortices produced are not enough to give a high
amount of particle deposition on the catalyst walls. The smallest
particles in the simulations, 5 and 10 nm particles were trapped to a
higher extent, than larger particles up to 1000 nm, in all studied
geometries with the predominant deposition mechanism being
Brownian diffusion. The comparison of the different filters designed
with a wall flow filter does show that the options for altering a design
of a flow through filter, without imposing a too large pressure drop
penalty are good.
Abstract: This paper will present the implementation of QoS
policy based system by utilizing rules on Access Control List (ACL)
over Layer 3 (L3) switch. Also presented is the architecture on that
implementation; the tools being used and the result were gathered.
The system architecture has an ability to control ACL rules which are
installed inside an external L3 switch. ACL rules used to instruct the
way of access control being executed, in order to entertain all traffics
through that particular switch. The main advantage of using this
approach is that the single point of failure could be prevented when
there are any changes on ACL rules inside L3 switches. Another
advantage is that the agent could instruct ACL rules automatically
straight away based on the changes occur on policy database without
configuring them one by one. Other than that, when QoS policy
based system was implemented in distributed environment, the
monitoring process can be synchronized easily due to the automate
process running by agent over external policy devices.
Abstract: Retrieval of the surface reflectance is important in the
remotely sensed data analysis to obtain the atmospheric reflectance or
atmospheric correction. The relationship between visible and mid
infrared reflectance over land was investigated and developed in this
study. The surface reflectances of the two visible bands were
measured using a handheld spectroradiometer collected around
Penang Island. In this study, we use the assumption that the 2.1 μm
band is not affected by aerosol and it is transparent to most aerosol
types (except dust). Therefore the satellite observed signal is the
same as the surface signal in 2.1 μm band. The correlation between
the surface reflectance measured by the spectroradiometer in the blue
and red region and the 2.1 μm observed by the satellite has been
established. We investigate five dates of Landsat TM scenes in this
study. The finding obtained by this study indicates that the surface
reflectance can be retrieved from the 2.1 μm band.
Abstract: Urban planning, in particular on protected landscape
areas, demands an increasing role of public participation within the
frame of the efficiency of sustainable planning process. The
development of urban planning actions in Protected Landscape areas,
as Sintra-Cascais Natural Park, should perform a methodological
process that is structured over distinct sequential stages, providing
the development of a continuous, interactive, integrated and
participative planning. From the start of Malveira da Serra and Janes
Plan process, several public participation actions were promoted, in
order to involve the local agents, stakeholders and the population in
the decision of specific local key issues and define the appropriate
priorities within the goals and strategies previously settled. As a
result, public participation encouraged an innovative process that
guarantees the efficiency of sustainable urban planning and promotes
a sustainable new way of living in community.
Abstract: In this article, we discuss project-based learning in the context of a wheel garden as an instructional tool in science and mathematics education. A wheel garden provides multiple opportunities to teach across the curriculum, to integrate disciplines, and to promote community involvement. Grounded in the theoretical framework of constructivism, the wheel garden provides a multidisciplined educational tool that provides a hands-on, non-traditional arena for learning. We will examine some of the cultural, art, science, and mathematics connections made with this project.
Abstract: one of the significant factors for improving the
accuracy of Land Surface Temperature (LST) retrieval is the correct
understanding of the directional anisotropy for thermal radiance. In
this paper, the multiple scattering effect between heterogeneous
non-isothermal surfaces is described rigorously according to the
concept of configuration factor, based on which a directional thermal
radiance model is built, and the directional radiant character for urban
canopy is analyzed. The model is applied to a simple urban canopy
with row structure to simulate the change of Directional Brightness
Temperature (DBT). The results show that the DBT is aggrandized
because of the multiple scattering effects, whereas the change range of
DBT is smoothed. The temperature difference, spatial distribution,
emissivity of the components can all lead to the change of DBT. The
“hot spot" phenomenon occurs when the proportion of high
temperature component in the vision field came to a head. On the other
hand, the “cool spot" phenomena occur when low temperature
proportion came to the head. The “spot" effect disappears only when
the proportion of every component keeps invariability. The model
built in this paper can be used for the study of directional effect on
emissivity, the LST retrieval over urban areas and the adjacency effect
of thermal remote sensing pixels.
Abstract: The integration between technology of remote
sensing, information from the data of digital image, and modeling
technology for the simulation of water quality will provide easiness
during the observation on the quality of water changes on the river
surface. For example, Ciliwung River which is contaminated with
non-point source pollutant from household wastes, particularly on its
downstream. This fact informed that the quality of water in this river
is getting worse. The land use for settlements and housing ranges
between 62.84% - 81.26% on the downstream of Ciliwung River,
give a significant picture in seeing factors that affected the water
quality of Ciliwung River.
Abstract: Calcite aCalcite and aragonite are the two common
polymorphs of CaCO3 observed as biominerals. It is universal that
the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM).
In vivo crystallization, Mg2+ inhibits calcite formation. For this
reason, stony corals skeleton may be formed only aragonite crystals
in the biocalcification. It is special in case of soft corals of which
formed only calcite crystal; however, this interesting phenomenon,
still uncharacterized in the marine environment, has been explored in
this study using newly purified cell-free proteins isolated from the
endoskeletal sclerites of soft coral. By recording the decline of pH in
vitro, the control of CaCO3 nucleation and crystal growth by the cellfree
proteins was revealed. Using Atomic Force Microscope, here we
find that these endoskeletal cell-free proteins significantly design the
morphological shape in the molecular-scale kinetics of crystal
formation and those proteins act as surfactants to promote ion
attachment at calcite steps.nd aragonite are the two common polymorphs of CaCO3 observed as biominerals. It is universal that the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM). In vivo crystallization, Mg2+ inhibits calcite formation. For this reason, stony corals skeleton may be formed only aragonite crystals in the biocalcification. It is special in case of soft corals of which formed only calcite crystal; however, this interesting phenomenon, still uncharacterized in the marine environment, has been explored in this study using newly purified cell-free proteins isolated from the endoskeletal sclerites of soft coral. By recording the decline of pH in vitro, the control of CaCO3 nucleation and crystal growth by the cell-free proteins was revealed. Using Atomic Force Microscope, here we find that these endoskeletal cell-free proteins significantly design the morphological shape in the molecular-scale kinetics of crystal formation and those proteins act as surfactants to promote ion attachment at calcite steps. KeywordsBiomineralization, Calcite, Cell-free protein, Soft coral
Abstract: The structural interpretation of a part of eastern Potwar
(Missa Keswal) has been carried out with available seismological,
seismic and well data. Seismological data contains both the source
parameters and fault plane solution (FPS) parameters and seismic data
contains ten seismic lines that were re-interpreted by using well data.
Structural interpretation depicts two broad types of fault sets namely,
thrust and back thrust faults. These faults together give rise to pop up
structures in the study area and also responsible for many structural
traps and seismicity. Seismic interpretation includes time and depth
contour maps of Chorgali Formation while seismological interpretation
includes focal mechanism solution (FMS), depth, frequency,
magnitude bar graphs and renewal of Seismotectonic map. The Focal
Mechanism Solutions (FMS) that surrounds the study area are
correlated with the different geological and structural maps of the area
for the determination of the nature of subsurface faults. Results of
structural interpretation from both seismic and seismological data
show good correlation. It is hoped that the present work will help in
better understanding of the variations in the subsurface structure and
can be a useful tool for earthquake prediction, planning of oil field and
reservoir monitoring.
Abstract: The study of cytokine expression in mice under the
influence of inactivated poliovirus and Imovaks polio vaccine in
combination with derivatives of chitosan shows various kinds of
processes. There is a significant increase in IL-12 in the serum of
immunized animals, which should stimulate the production of IFN-γ
NK-cells and T-cells and polarize the immune response to Th1 type.
Thus, the derivatives of chitosan can promote cell component of the
immune response, providing a full antiviral immunity.
Abstract: Mixed Reality (MR) is one of the newest technologies
explored in education. It promises the potential to promote teaching
and learning and making learners- experience more “engaging".
However, there still lack of research on designing a virtual learning
environment using MR technology. In this paper, we describe the
Mixed Reality technology, the characteristics of situated learning as
instructional design for virtual environment using mixed reality
technology. We also explain a case study that implemented those
design and also the system overview.
Abstract: The New Basel Capital Accord (Basel II) influences how financial institutions around the world, and especially European Union institutions, determine the amount of capital to reserve. However, as the recent global crisis has shown, the revision of Basel II is needed to reflect current trends, such as increased volatility and correlation, in the world financial markets. The overall objective of Basel II is to increase the safety and soundness of the international financial system. Basel II builds on three main pillars: Pillar I deals with the minimum capital requirements for credit, market and operational risk, Pillar II focuses on the supervisory review process and finally Pillar III promotes market discipline through enhanced disclosure requirements for banks. The aim of this paper is to provide the historical background, key features and impact of Basel II on financial markets. Moreover, we discuss new proposals for international bank regulation (sometimes referred to as Basel III) which include requirements for higher quality, constituency and transparency of banks' capital and risk management, regulation of OTC markets and introduction of new liquidity standards for internationally active banks.
Abstract: The Taiwan government has started to promote the “Plain Landscape Afforestation and Greening Program" since 2002. A key task of the program was the payment for environmental services (PES), entitled the “Plain Landscape Afforestation Policy" (PLAP), which was certificated by the Executive Yuan on August 31, 2001 and enacted on January 1, 2002. According to the policy, it is estimated that the total area of afforestation will be 25,100 hectares by December 31, 2007. Until the end of 2007, the policy had been enacted for six years in total and the actual area of afforestation was 8,919.18 hectares. Among them, Taiwan Sugar Corporation (TSC) was accounted for 7,960 hectares (with 2,450.83 hectares as public service area) which occupied 86.22% of the total afforestation area; the private farmland promoted by local governments was accounted for 869.18 hectares which occupied 9.75% of the total afforestation area. Based on the above, we observe that most of the afforestation area in this policy is executed by TSC, and the achievement ratio by TSC is better than by others. It implies that the success of the PLAP is seriously related to the execution of TSC. The objective of this study is to analyze the relevant policy planning of TSC's participation in the PLAP, suggest complementary measures, and draw up effective adjustment mechanisms, so as to improve the effectiveness of executing the policy. Our main conclusions and suggestions are summarized as follows: 1. The main reason for TSC’s participation in the PLAP is based on their passive cooperation with the central government or company policy. Prior to TSC’s participation in the PLAP, their lands were mainly used for growing sugarcane. 2. The main factors of TSC's consideration on the selection of tree species are based on the suitability of land and species. The largest proportion of tree species is allocated to economic forests, and the lack of technical instruction was the main problem during afforestation. Moreover, the method of improving TSC’s future development in leisure agriculture and landscape business becomes a key topic. 3. TSC has developed short and long-term plans on participating in the PLAP for the future. However, there is no great willingness or incentive on budgeting for such detailed planning. 4. Most people from TSC interviewed consider the requirements on PLAP unreasonable. Among them, an unreasonable requirement on the number of trees accounted for the greatest proportion; furthermore, most interviewees suggested that the government should continue to provide incentives even after 20 years. 5. Since the government shares the same goals as TSC, there should be sufficient cooperation and communication that support the technical instruction and reduction of afforestation cost, which will also help to improve effectiveness of the policy.
Abstract: Using Internet communication, new home electronics
have functions of monitoring and control from remote. However in
many case these electronics work as standalone, and old electronics
are not followed. Then, we developed the total remote system include
not only new electronics but olds. This systems node is a adapter of
electrical power plug that embed relay switch and some sensors, and
these nodes communicate with each other. the system server was build
on the Internet, and users access to this system from web browsers.
To reduce the cost to set up of this system, communication between
adapters are used ZigBee wireless network instead of wired LAN
cable[3]. From measured RSSI(received signal strength indicator)
information between each nodes, the system can estimate roughly
adapters were mounted on which room, and where in the room. So
also it reduces the cost of mapping nodes. Using this system, energy
saving and house monitoring are expected.
Abstract: On one hand, SNMP (Simple Network Management
Protocol) allows integrating different enterprise elements connected
through Internet into a standardized remote management. On the
other hand, as a consequence of the success of Intelligent Houses
they can be connected through Internet now by means of a residential
gateway according to a common standard called OSGi (Open
Services Gateway initiative). Due to the specifics of OSGi Service
Platforms and their dynamic nature, specific design criterions should
be defined to implement SNMP Agents for OSGi in order to integrate
them into the SNMP remote management. Based on the analysis of
the relation between both standards (SNMP and OSGi), this paper
shows how OSGi Service Platforms can be included into the SNMP
management of a global enterprise, giving implementation details
about an SNMP Agent solution and the definition of a new MIB
(Management Information Base) for managing OSGi platforms that
takes into account the specifics and dynamic nature of OSGi.
Abstract: Atherosclerosis is the condition in which an artery
wall thickens as the result of a build-up of fatty materials such as
cholesterol. It is a syndrome affecting arterial blood vessels, a
chronic inflammatory response in the walls of arteries, in large part
due to the accumulation of macrophage white blood cells and
promoted by low density (especially small particle) lipoproteins
(plasma proteins that carry cholesterol and triglycerides) without
adequate removal of fats and cholesterol from the macrophages by
functional high density lipoproteins (HDL). It is commonly referred
to as a hardening or furring of the arteries. It is caused by the
formation of multiple plaques within the arteries.
Abstract: We present an integration approach of a CMOS biosensor into a polymer based microfluidic environment suitable for mass production. It consists of a wafer-level-package for the silicon die and laser bonding process promoted by an intermediate hot melt foil to attach the sensor package to the microfluidic chip, without the need for dispensing of glues or underfiller. A very good condition of the sensing area was obtained after introducing a protection layer during packaging. A microfluidic flow cell was fabricated and shown to withstand pressures up to Δp = 780 kPa without leakage. The employed biosensors were electrically characterized in a dry environment.
Abstract: According to dramatic growth of internet services, an easy and prompt service deployment has been important for internet service providers to successfully maintain time-to-market. Before global service deployment, they have to pay the big cost for service evaluation to make a decision of the proper system location, system scale, service delay and so on. But, intra-Lab evaluation tends to have big gaps in the measured data compared with the realistic situation, because it is very difficult to accurately expect the local service environment, network congestion, service delay, network bandwidth and other factors. Therefore, to resolve or ease the upper problems, we propose multiple cloud based GPES Broker system and use case that helps internet service providers to alleviate the above problems in beta release phase and to make a prompt decision for their service launching. By supporting more realistic and reliable evaluation information, the proposed GPES Broker system saves the service release cost and enables internet service provider to make a prompt decision about their service launching to various remote regions.