Abstract: A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 - kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide α- factor, under the control of AOX1 promoter. The recombined plasmid was transformed into the host strain P. pastoris KM71H and X33 by electroporation. Both host strains could efficiently express and secret phytase. The multicopy strains were screened for over expression of phytase. All the selected multicopy strains of P. pastoris X33 were examined for phytase activity, the maximum phytase yield of 1329 IU/ml was obtained after 4 days of incubation in medium BMM. The recombinant protein with MW of 97.4 KW showed to be the only one protein secreted in the culture broth. Multicopy transformant P. pastoris X33 supposed to be potential candidate for producing the commercial preparation of phytase.
Abstract: This study presents the numerical simulation of
optimum pin-fin heat sink with air impinging cooling by using
Taguchi method. 9 L ( 4 3 ) orthogonal array is selected as a plan for
the four design-parameters with three levels. The governing
equations are discretized by using the
control-volume-based-finite-difference method with a power-law
scheme on the non-uniform staggered grid. We solved the coupling of
the velocity and the pressure terms of momentum equations using
SIMPLEC algorithm. We employ the k −ε two-equations
turbulence model to describe the turbulent behavior. The parameters
studied include fin height H (35mm-45mm), inter-fin spacing a , b ,
and c (2 mm-6.4 mm), and Reynolds number ( Re = 10000- 25000).
The objective of this study is to examine the effects of the fin
spacings and fin height on the thermal resistance and to find the
optimum group by using the Taguchi method. We found that the fin
spacings from the center to the edge of the heat sink gradually
extended, and the longer the fin’s height the better the results. The
optimum group is 3 1 2 3 H a b c . In addition, the effects of parameters are
ranked by importance as a , H , c , and b .
Abstract: Sustainability in rural production system can only be achieved if it can suitably satisfy the local requirement as well as the outside demand with the changing time. With the increased pressure from the food sector in a globalised world, the agrarian economy
needs to re-organise its cultivable land system to be compatible with new management practices as well as the multiple needs of various stakeholders and the changing resource scenario. An attempt has been made to transform this problem into a multi-objective decisionmaking problem considering various objectives, resource constraints and conditional constraints. An interactive fuzzy multi-objective
programming approach has been used for such a purpose taking a
case study in Indian context to demonstrate the validity of the method.
Abstract: Fatigue tests of specimen-s with numerous holes are
presented. The tests were made up till fatigue cracks have been
created on both sides of the hole. Their extension was stopping with
pressed plastic deformation at the mouth of the detected crack. It is
shown that the moments of occurrence of cracks on holes are
stochastically dependent. This dependence has positive and negative
correlation relations. Shown that the positive correlation is formed
across of the applied force, while negative one – along it. The
negative relationship extends over a greater distance. The
mathematical model of dependence area formation is represented as
well as the estimating of model parameters. The positive correlation
of fatigue cracks origination can be considered as an extension of one
main crack. With negative correlation the first crack locates the place
of its origin, leading to the appearance of multiple cracks; do not
merge with each other.
Abstract: Deployment of pneumatic muscles in various
industrial applications is still in its early days, considering the relative
newness of these components. The field of robotics holds particular
future potential for pneumatic muscles, especially in view of their
specific behaviour known as compliance. The paper presents and
discusses an innovative constructive solution for a gripper system
mountable on an industrial robot, based on actuation by a linear
pneumatic muscle and transmission of motion by gear and rack
mechanism. The structural, operational and constructive models of
the new gripper are presented, along with some of the experimental
results obtained subsequently to the testing of a prototype. Further
presented are two control variants of the gripper system, one by
means of a 3/2-way fast-switching solenoid valve, the other by means
of a proportional pressure regulator. Advantages and disadvantages
are discussed for both variants.
Abstract: In this study, an optimization of supersonic air-to-air ejector is carried out by a recently developed single-objective genetic algorithm based on adaption of sequence of individuals. Adaptation of sequence is based on Shape-based distance of individuals and embedded micro-genetic algorithm. The optimal sequence found defines the succession of CFD-aimed objective calculation within each generation of regular micro-genetic algorithm. A spring-based deformation mutates the computational grid starting the initial individualvia adapted population in the optimized sequence. Selection of a generation initial individual is knowledge-based. A direct comparison of the newly defined and standard micro-genetic algorithm is carried out for supersonic air-to-air ejector. The only objective is to minimize the loose of total stagnation pressure in the ejector. The result is that sequence-adopted micro-genetic algorithm can provide comparative results to standard algorithm but in significantly lower number of overall CFD iteration steps.
Abstract: Discharges in hydrogen, ignited by wire explosion, with current amplitude up to 1.5 MA were investigated. Channel diameter oscillations were observed on the photostreaks. Voltage and current curves correlated with the photostreaks. At initial gas pressure of 5-35 MPa the oscillation period was proportional to square root of atomic number of the initiating wire material. These oscillations were associated with aligned magnetic and gas-kinetic pressures. At initial pressure of 80-160 MPa acoustic pressure fluctuations on the discharge chamber wall were increased up to 150 MPa and there were the growth of voltage fluctuations on the discharge gap up to 3 kV simultaneously with it. In some experiments it was observed abrupt increase in the oscillation amplitude, which can be caused by the resonance of the acoustic oscillations in discharge chamber volume and the oscillations connected with alignment of the gaskinetic pressure and the magnetic pressure, as far as frequencies of these oscillations are close to each other in accordance with the estimates and the experimental data. Resonance of different type oscillations can produce energy density increasing in the discharge channel. Thus, the appropriate initial conditions in the experiment allow to increase the energy density in the discharge channel
Abstract: In this work, Experimental tie-line results and
solubility (binodal) curves were obtained for the ternary systems
(water + acetic acid + methyl isobutyl ketone (MIBK)), (water +
lactic acid+ methyl isobutyl ketone) at T = 294.15K and atmospheric
pressure. The consistency of the values of the experimental tie-lines
was determined through the Othmer-Tobias and Hands correlations.
For the extraction effectiveness of solvents, the distribution and
selectivity curves were plotted. In addition, these experimental tieline
data were also correlated with NRTL model. The interaction
parameters for the NRTL model were retrieved from the obtained
experimental results by means of a combination of the homotopy
method and the genetic algorithms.
Abstract: Hydrodesulfurization (HDS) of dibenzothiophene
(DBT) in a high pressure batch reactor was done at 320 °C on
CoMoS/Al2O3-B2O3 (4, 10, and 16 wt. % of Boria) using nhexadecane
as solvent, dimethyldisulfide (DMDS) in tetradecane as
sulfur agent, and stirring at 1000 rpm. The effects of boria were
investigated by using X-ray diffraction (XRD), Temperature
programmed desorption (TPD) of ammonia, and Brunauer-Emmet-
Teller (BET) experiments. The results showed that the catalyst
prepared with low boria content (4 wt. %) had HDS activity (in
pseudo first order kinetic constant basis) value ~1.45 times higher to
that of CoMoS/Al2O3 catalyst.
Abstract: A talking head system (THS) is presented to animate
the face of a speaking 3D avatar in such a way that it realistically
pronounces the given Korean text. The proposed system consists of
SAPI compliant text-to-speech (TTS) engine and MPEG-4 compliant
face animation generator. The input to the THS is a unicode text that is
to be spoken with synchronized lip shape. The TTS engine generates a
phoneme sequence with their duration and audio data. The TTS
applies the coarticulation rules to the phoneme sequence and sends a
mouth animation sequence to the face modeler. The proposed THS can
make more natural lip sync and facial expression by using the face
animation generator than those using the conventional visemes only.
The experimental results show that our system has great potential for
the implementation of talking head for Korean text.
Abstract: The kinetics of palm oil catalytic cracking over
aluminum containing mesoporous silica Al-MCM-41 (5% Al) was
investigated in a batch autoclave reactor at the temperatures range of
573 – 673 K. The catalyst was prepared by using sol-gel technique
and has been characterized by nitrogen adsorption and x-ray
diffraction methods. Surface area of 1276 m2/g with average pore
diameter of 2.54 nm and pore volume of 0.811 cm3/g was obtained.
The experimental catalytic cracking runs were conducted using 50 g
of oil and 1 g of catalyst. The reaction pressure was recorded at
different time intervals and the data were analyzed using Levenberg-
Marquardt (LM) algorithm using polymath software. The results
show that the reaction order was found to be -1.5 and activation
energy of 3200 J/gmol.
Abstract: The steady incompressible flow has been solved in cylindrical coordinates in both vapour region and wick structure. The governing equations in vapour region are continuity, Navier-Stokes and energy equations. These equations have been solved using SIMPLE algorithm. For study of parameters variation on heat pipe operation, a benchmark has been chosen and the effect of changing one parameter has been analyzed when the others have been fixed.
Abstract: Fuzzy logic system (FLS) is used in this study to
predict the tractive performance in terms of traction force, and
motion resistance for an intelligent air cushion track vehicle while it
operates in the swamp peat. The system is effective to control the
intelligent air –cushion system with measuring the vehicle traction
force (TF), motion resistance (MR), cushion clearance height (CH)
and cushion pressure (CP). Ultrasonic displacement sensor, pull-in
solenoid electromagnetic switch, pressure control sensor, micro
controller, and battery pH sensor are incorporated with the Fuzzy
logic system to investigate experimentally the TF, MR, CH, and CP.
In this study, a comparison for tractive performance of an intelligent
air cushion track vehicle has been performed with the results obtained
from the predicted values of FLS and experimental actual values. The
mean relative error of actual and predicted values from the FLS
model on traction force, and total motion resistance are found as 5.58
%, and 6.78 % respectively. For all parameters, the relative error of
predicted values are found to be less than the acceptable limits. The
goodness of fit of the prediction values from the FLS model on TF,
and MR are found as 0.90, and 0.98 respectively.
Abstract: In this study, the precision heading process of
spur gears has been investigated by means of numerical
analysis. The effect of some parameters such as teeth number
and module on the forming force and material flow were
presented. The simulation works were performed rigid-plastic
finite element method using DEFORM 3D software. In order
to validate the estimated numerical results, they were
compared with those obtained experimentally during heading
of spur gear using lead as a model material. Results showed
that the optimum number of gear teeth is between 10 to 20,
that is because of being the specific pressure in its minimum
value.
Abstract: Tumour suppressors are key participants in the
prevention of cancer. Regulation of their expression through
miRNAs is important for comprehensive translation inhibition of
tumour suppressors and elucidation of carcinogenesis mechanisms.
We studies the possibility of 1521 miRNAs to bind with 873 mRNAs
of human tumour suppressors using RNAHybrid 2.1 and ERNAhybrid
programmes. Only 978 miRNAs were found to be
translational regulators of 812 mRNAs, and 61 mRNAs did not have
any miRNA binding sites. Additionally, 45.9% of all miRNA binding
sites were located in coding sequences (CDSs), 33.8% were located
in 3' untranslated region (UTR), and 20.3% were located in the
5'UTR. MiRNAs binding with more than 50 target mRNAs and
mRNAs binding with several miRNAs were selected. Hsa-miR-5096
had 15 perfectly complementary binding sites with mRNAs of 14
tumour suppressors. These newly indentified miRNA binding sites
can be used in the development of medicines (anti-sense therapies)
for cancer treatment.
Abstract: This article investigates a contribution of synthesized visual speech. Synthesis of visual speech expressed by a computer consists in an animation in particular movements of lips. Visual speech is also necessary part of the non-manual component of a sign language. Appropriate methodology is proposed to determine the quality and the accuracy of synthesized visual speech. Proposed methodology is inspected on Czech speech. Hence, this article presents a procedure of recording of speech data in order to set a synthesis system as well as to evaluate synthesized speech. Furthermore, one option of the evaluation process is elaborated in the form of a perceptual test. This test procedure is verified on the measured data with two settings of the synthesis system. The results of the perceptual test are presented as a statistically significant increase of intelligibility evoked by real and synthesized visual speech. Now, the aim is to show one part of evaluation process which leads to more comprehensive evaluation of the sign speech synthesis system.
Abstract: An actual power plant, which is the power plant of Iron and Steel Factory at Misurata city in Libya , has been modeled using Matlab in order to compare its results to the actual results of the actual cycle. This paper concentrates on two factors:
a- The comparison between exergy losses in the actual cycle and the modeled cycle.
b- The effect of extracting pressure on temperature water at boiler inlet.
Closed heat exchangers used in this plant have been substituted by open heat exchangers in the current study of the modeled power plant and the required changes in the pressure have been considered. In the following investigation the two points mentioned above are taken in consideration.
Abstract: Accounts of language acquisition differ significantly in their treatment of the role of prediction in language learning. In particular, nativist accounts posit that probabilistic learning about words and word sequences has little to do with how children come to use language. The accuracy of this claim was examined by testing whether distributional probabilities and frequency contributed to how well 3-4 year olds repeat simple word chunks. Corresponding chunks were the same length, expressed similar content, and were all grammatically acceptable, yet the results of the study showed marked differences in performance when overall distributional frequency varied. It was found that a distributional model of language predicted the empirical findings better than a number of other models, replicating earlier findings and showing that children attend to distributional probabilities in an adult corpus. This suggested that language is more prediction-and-error based, rather than on abstract rules which nativist camps suggest.
Abstract: Intradiscal and intervertebral pressure transducers
were developed. They were used to map the pressures in the nucleus
and within the annulus of the human spinal segments. Their stressrelaxation
were recorded over a period of time for nucleus
pressure, applied load, and peripherial strain against time. The
results show that for normal discs, pressures in the nucleus are
viscoelastic in nature with the applied compressive load.
Mechanical strains which develop around the periphery of the
vertebral body are also viscoelastic with the applied compressive
load. Applied compressive load against time also shows viscoelastic
behavior. However, annulus does not respond viscoelastically with
the applied load. It showed a linear response to compressive loading.
Abstract: In a state-of-the-art industrial production line of
photovoltaic products the handling and automation processes are of
particular importance and implication. While processing a fully
functional crystalline solar cell an as-cut photovoltaic wafer is subject
to numerous repeated handling steps. With respect to stronger
requirements in productivity and decreasing rejections due to defects
the mechanical stress on the thin wafers has to be reduced to a
minimum as the fragility increases by decreasing wafer thicknesses.
In relation to the increasing wafer fragility, researches at the
Fraunhofer Institutes IPA and CSP showed a negative correlation
between multiple handling processes and the wafer integrity. Recent
work therefore focused on the analysis and optimization of the dry
wafer stack separation process with compressed air. The achievement
of a wafer sensitive process capability and a high production
throughput rate is the basic motivation in this research.