Abstract: Two commercial proteases from Bacillus
licheniformis (Alcalase 2.4 L FG and Alcalase 2.5 L, Type DX) were
screened for the production of Z-Ala-Phe-NH2 in batch reaction.
Alcalase 2.4 L FG was the most efficient enzyme for the C-terminal
amidation of Z-Ala-Phe-OMe using ammonium carbamate as
ammonium source. Immobilization of protease has been achieved by
the sol-gel method, using dimethyldimethoxysilane (DMDMOS) and
tetramethoxysilane (TMOS) as precursors (unpublished results). In
batch production, about 95% of Z-Ala-Phe-NH2 was obtained at
30°C after 24 hours of incubation. Reproducibility of different
batches of commercial Alcalase 2.4 L FG preparations was also
investigated by evaluating the amidation activity and the entrapment
yields in the case of immobilization. A packed-bed reactor (0.68 cm
ID, 15.0 cm long) was operated successfully for the continuous
synthesis of peptide amides. The immobilized enzyme retained the
initial activity over 10 cycles of repeated use in continuous reactor at
ambient temperature. At 0.75 mL/min flow rate of the substrate
mixture, the total conversion of Z-Ala-Phe-OMe was achieved after 5
hours of substrate recycling. The product contained about 90%
peptide amide and 10% hydrolysis byproduct.
Abstract: A boundary layer wind tunnel facility has been
adopted in order to conduct experimental measurements of the flow field around a model of the Panorama Giustinelli Building, Trieste
(Italy). Information on the main flow structures has been obtained by means of flow visualization techniques and has been compared to the
numerical predictions of the vortical structures spread on top of the roof, in order to investigate the optimal positioning for a vertical-axis
wind energy conversion system, registering a good agreement between experimental measurements and numerical predictions.
Abstract: In this paper, the direct AnsAz method is used for constructing the multi-wave solutions to the (2+1)-dimensional extension of the Korteweg de-Vries (shortly EKdV) equation. A new breather type of three-wave solutions including periodic breather type soliton solution, breather type of two-solitary solution are obtained. Some cases with specific values of the involved parameters are plotted for each of the three-wave solutions. Mechanical features of resonance interaction among the multi-wave are discussed. These results enrich the variety of the dynamics of higher-dimensional nonlinear wave field.
Abstract: The acidity of different raw Jordanian clays
containing zeolite, bentonite, red and white kaolinite and diatomite
was characterized by means of temperature programmed desorption
(TPD) of ammonia, conversion of 2-methyl-3-butyn-2-ol (MBOH),
FTIR and BET-measurements. FTIR spectra proved presence of
silanol and bridged hydroxyls on the clay surface. The number of
acidic sites was calculated from experimental TPD-profiles. We
observed the decrease of surface acidity correlates with the decrease
of Si/Al ratio except for diatomite. On the TPD-plot for zeolite two
maxima were registered due to different strength of surface acidic
sites. Values of MBOH conversion, product yields and selectivity
were calculated for the catalysis on Jordanian clays. We obtained that
all clay samples are able to convert MBOH into a major product
which is 3-methyl-3-buten-1-yne (MBYNE) catalyzed by acid
surface sites with the selectivity close to 70%. There was found a
correlation between MBOH conversion and acidity of clays
determined by TPD-NH3, i.e. the higher the acidity the higher the
conversion of MBOH. However, diatomite provided the lowest
conversion of MBOH as result of poor polarization of silanol groups.
Comparison of surface areas and conversions revealed the highest
density of active sites for red kaolinite and the lowest for zeolite and
diatomite.
Abstract: Cryptography provides the secure manner of
information transmission over the insecure channel. It authenticates
messages based on the key but not on the user. It requires a lengthy
key to encrypt and decrypt the sending and receiving the messages,
respectively. But these keys can be guessed or cracked. Moreover,
Maintaining and sharing lengthy, random keys in enciphering and
deciphering process is the critical problem in the cryptography
system. A new approach is described for generating a crypto key,
which is acquired from a person-s iris pattern. In the biometric field,
template created by the biometric algorithm can only be
authenticated with the same person. Among the biometric templates,
iris features can efficiently be distinguished with individuals and
produces less false positives in the larger population. This type of iris
code distribution provides merely less intra-class variability that aids
the cryptosystem to confidently decrypt messages with an exact
matching of iris pattern. In this proposed approach, the iris features
are extracted using multi resolution wavelets. It produces 135-bit iris
codes from each subject and is used for encrypting/decrypting the
messages. The autocorrelators are used to recall original messages
from the partially corrupted data produced by the decryption process.
It intends to resolve the repudiation and key management problems.
Results were analyzed in both conventional iris cryptography system
(CIC) and non-repudiation iris cryptography system (NRIC). It
shows that this new approach provides considerably high
authentication in enciphering and deciphering processes.
Abstract: The large and small-scale shaking table tests, which
was conducted for investigating damage evolution of piles inside
liquefied soil, are numerically simulated and experimental verified by the3D nonlinear finite element analysis. Damage evolution of
elasto-plastic circular steel piles and reinforced concrete (RC) one with cracking and yield of reinforcement are focused on, and the failure patterns and residual damages are captured by the proposed constitutive models. The superstructure excitation behind quay wall is
reproduced as well.
Abstract: Extraction of laccase produced by L. polychrous in an
aqueous two-phase system, composed of polyethylene glycol and
phosphate salt at pH 7.0 and 250C was investigated. The effect of
PEG molecular weight, PEG concentration and phosphate
concentration was determined. Laccase preferentially partitioned to
the top phase. Good extraction of laccase to the top phase was
observed with PEG 4000. The optimum system was found in the
system containing 12% w/w PEG 4000 and 16% w/w phosphate salt
with KE of 88.3, purification factor of 3.0-fold and 99.1% yield.
Some properties of the enzyme such as thermal stability, effect of
heavy metal ions and kinetic constants were also presented in this
work. The thermal stability decreased sharply with high temperature
above 60 0C. The enzyme was inhibited by Cd2+, Pb2+, Zn2+ and
Cu2+. The Vmax and Km values of the enzyme were 74.70
μmol/min/ml and 9.066 mM respectively.
Abstract: wind catchers have been served as a cooling system, used to provide acceptable ventilation by means of renewable energy of wind. In the present study, the city of Yazd in arid climate is selected as case study. From the architecture point of view, learning about wind catchers in this study is done by means of field surveys. Research method for selection of the case is based on random form, and analytical method. Wind catcher typology and knowledge of relationship governing the wind catcher's architecture were those measures that are taken for the first time. 53 wind catchers were analyzed. The typology of the wind-catchers is done by the physical analyzing, patterns and common concepts as incorporated in them. How the architecture of wind catcher can influence their operations by analyzing thermal behavior are the archetypes of selected wind catchers. Calculating fluids dynamics science, fluent software and numerical analysis are used in this study as the most accurate analytical approach. The results obtained from these analyses show the formal specifications of wind catchers with optimum operation in Yazd. The knowledge obtained from the optimum model could be used for design and construction of wind catchers with more improved operation
Abstract: Friction-stir welding has received a huge interest in the last few years. The many advantages of this promising process have led researchers to present different theoretical and experimental explanation of the process. The way to quantitatively and qualitatively control the different parameters of the friction-stir welding process has not been paved. In this study, a refined energybased model that estimates the energy generated due to friction and plastic deformation is presented. The effect of the plastic deformation at low energy levels is significant and hence a scale factor is introduced to control its effect. The predicted heat energy and the obtained maximum temperature using our model are compared to the theoretical and experimental results available in the literature and a good agreement is obtained. The model is applied to AA6000 and AA7000 series.
Abstract: Tumor classification is a key area of research in the
field of bioinformatics. Microarray technology is commonly used in
the study of disease diagnosis using gene expression levels. The
main drawback of gene expression data is that it contains thousands
of genes and a very few samples. Feature selection methods are used
to select the informative genes from the microarray. These methods
considerably improve the classification accuracy. In the proposed
method, Genetic Algorithm (GA) is used for effective feature
selection. Informative genes are identified based on the T-Statistics,
Signal-to-Noise Ratio (SNR) and F-Test values. The initial candidate
solutions of GA are obtained from top-m informative genes. The
classification accuracy of k-Nearest Neighbor (kNN) method is used
as the fitness function for GA. In this work, kNN and Support Vector
Machine (SVM) are used as the classifiers. The experimental results
show that the proposed work is suitable for effective feature
selection. With the help of the selected genes, GA-kNN method
achieves 100% accuracy in 4 datasets and GA-SVM method
achieves in 5 out of 10 datasets. The GA with kNN and SVM
methods are demonstrated to be an accurate method for microarray
based tumor classification.
Abstract: The relevance of the study of everyday life in Almaty
and Kyzylorda are associated with the emergence of the modern
trends in historiography and socializing areas of government reform.
The relevance is due to the fact that in the early twentieth century
Kyzylorda and Almaty began to develop as a city and this period has
a special place in the life of the state. An interesting aspect of the
everyday life of the inhabitants of the new city, which was built in the
era of Stalin's Five-Year Plans, can be examined through the eyes of
the Soviet people living in a specific environment, reflecting the life
of the citizens. The study of industrialization of the Soviet Union and
the attention paid to new developments in the first five years of
everyday aspects as the impact of the modernization of the 1930s was
one of the decisive factors in the lives of residents. Among these
factors, we would like to highlight the medical field, which is the
basis of all human life, specifically focusing on the state of medicine
in Alma-Ata in the first 20-30-years of the twentieth century, and
analyze the different aspects of human life, determining the quality of
medical care to the population during this period.
Abstract: COSMED K4b2 is a portable electrical device designed to test pulmonary functions. It is ideal for many applications that need the measurement of the cardio-respiratory response either in the field or in the lab is capable with the capability to delivery real time data to a sink node or a PC base station with storing data in the memory at the same time. But the actual sensor outputs and data received may contain some errors, such as impulsive noise which can be related to sensors, low batteries, environment or disturbance in data acquisition process. These abnormal outputs might cause misinterpretations of exercise or living activities to persons being monitored. In our paper we propose an effective and feasible method to detect and identify errors in applications by principal component analysis (PCA) and a back propagation (BP) neural network.
Abstract: This research paper is based upon the simulation of
gradient of mathematical functions and scalar fields using MATLAB.
Scalar fields, their gradient, contours and mesh/surfaces are
simulated using different related MATLAB tools and commands for
convenient presentation and understanding. Different mathematical
functions and scalar fields are examined here by taking their
gradient, visualizing results in 3D with different color shadings and
using other necessary relevant commands. In this way the outputs of
required functions help us to analyze and understand in a better way
as compared to just theoretical study of gradient.
Abstract: Consumer behaviour analysis represents an important
field of study in marketing. Particularly strategy development for
marketing and communications will be more focused and effective
when marketers have an understanding of the motivations, behaviour
and psychology of consumers. While materialism has been found to
be one of the important elements in consumer behaviour, compulsive
consumption represents another aspect that has recently attracted
more attention. This is because of the growing prevalence of
dysfunctional buying that has raised concern in consumer societies.
Present studies and analyses on origins and motivations of
compulsive buying have mainly focused on either individual factors
or groups of related factors and hence a need for a holistic view
exists. This paper provides a comprehensive perspective on
compulsive consumption and establishes relevant propositions
keeping the family life cycle stages as a reference for the incidence of
chronic consumer states and their influence on compulsive
consumption.
Abstract: An attempt was made to study of nitrogen
components response of corn (Zea mays L.) to drought stress. A farm
research was done in RCBD as split-plot with four replications in
Khorramabad, west Iran. Drought stress levels as irrigation regimes
after 75 (control), 100, and 120 (stress) mm cumulative evaporation
were in main plots, and four seed corn varieties include 500 (medium
maturity), 647, 700, and 704 (long maturity) were as subplots.
Soluble protein, nitrate and proline amino acid were measured in
shoot and root at flowering stage, and grain yield was measured in
harvesting stage. As the drought progressed, the amount of nitrate
and proline followed an increasing trend, but soluble protein
decreased in shoot and root. The highest amount of nitrate and
proline was observed in longer maturity varieties than shorter ones,
but decrease yield of long maturity varieties was higher than medium
maturity varieties in drought condition, because of long duration of
stress.
Abstract: Environmental micro-organisms include a large number of taxa and some species that are generally considered nonpathogenic, but can represent a risk in certain conditions, especially for elderly people and immunocompromised individuals. Chemotaxonomic identification techniques are powerful tools for environmental micro-organisms, and cellular fatty acid methyl esters (FAME) content is a powerful fingerprinting identification technique. A system based on an unsupervised artificial neural network (ANN) was set up using the fatty acid profiles of standard bacterial strains, obtained by gas-chromatography, used as learning data. We analysed 45 certified strains belonging to Acinetobacter, Aeromonas, Alcaligenes, Aquaspirillum, Arthrobacter, Bacillus, Brevundimonas, Enterobacter, Flavobacterium, Micrococcus, Pseudomonas, Serratia, Shewanella and Vibrio genera. A set of 79 bacteria isolated from a drinking water line (AMGA, the major water supply system in Genoa) were used as an example for identification compared to standard MIDI method. The resulting ANN output map was found to be a very powerful tool to identify these fresh isolates.
Abstract: To model the human visual system (HVS) in the region of interest, we propose a new objective metric evaluation adapted to wavelet foveation-based image compression quality measurement, which exploits a foveation setup filter implementation technique in the DWT domain, based especially on the point and region of fixation of the human eye. This model is then used to predict the visible divergences between an original and compressed image with respect to this region field and yields an adapted and local measure error by removing all peripheral errors. The technique, which we call foveation wavelet visible difference prediction (FWVDP), is demonstrated on a number of noisy images all of which have the same local peak signal to noise ratio (PSNR), but visibly different errors. We show that the FWVDP reliably predicts the fixation areas of interest where error is masked, due to high image contrast, and the areas where the error is visible, due to low image contrast. The paper also suggests ways in which the FWVDP can be used to determine a visually optimal quantization strategy for foveation-based wavelet coefficients and to produce a quantitative local measure of image quality.
Abstract: Within the realm of e-government, the development has moved towards testing new means for democratic decisionmaking, like e-panels, electronic discussion forums, and polls. Although such new developments seem promising, they are not problem-free, and the outcomes are seldom used in the subsequent formal political procedures. Nevertheless, process models offer promising potential when it comes to structuring and supporting transparency of decision processes in order to facilitate the integration of the public into decision-making procedures in a reasonable and manageable way. Based on real-life cases of urban planning processes in Sweden, we present an outline for an integrated framework for public decision making to: a) provide tools for citizens to organize discussion and create opinions; b) enable governments, authorities, and institutions to better analyse these opinions; and c) enable governments to account for this information in planning and societal decision making by employing a process model for structured public decision making.
Abstract: Glutathione S-transferase was purified from human
erythrocytes and effects of some polyphenols were investigated on
the enzyme activity. The purification procedure was performed on
Glutathione-Agarose affinity chromatography after preparation of
erythrocytes hemolysate with a yield of 81%. The purified enzyme
showed a single band on the SDS-PAGE. The effects of some
poliphenolic compounds such as catechin, dopa, dopamine, progallol
and catechol were examined on the in vitro GST activity. Catechin
was determined to be inhibitor for the enzyme, but others were not
effective on the enzyme as inhibitors or activators. IC50 value -the
concentration of inhibitor which reduces enzyme activity by 50%-
was estimated to be 10 mM. Ki constants were also calculated as 6.38
± 0,70 mM with GSH substrate, and 3.86 ± 0,78 mM with CDNB
substrate using the equations of graphs for the inhibitor, and its
inhibition type was determined as non-competitive.
Abstract: A reduced order modeling approach for natural
gas transient flow in pipelines is presented. The Euler
equations are considered as the governing equations and
solved numerically using the implicit Steger-Warming flux
vector splitting method. Next, the linearized form of the
equations is derived and the corresponding eigensystem is
obtained. Then, a few dominant flow eigenmodes are used to
construct an efficient reduced-order model. A well-known test
case is presented to demonstrate the accuracy and the
computational efficiency of the proposed method. The results
obtained are in good agreement with those of the direct
numerical method and field data. Moreover, it is shown that
the present reduced-order model is more efficient than the
conventional numerical techniques for transient flow analysis
of natural gas in pipelines.