Contrast-Enhanced Multispectal Upconversion Fluorescence Analysis for High-Resolution in-vivo Deep Tissue Imaging

Lanthanide-doped upconversion nanoparticles which can convert near-infrared lights to visible lights have attracted growing interest because of their great potentials in fluorescence imaging. Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. However, improving the detection sensitivity and decreasing the absorption and scattering in biological tissues are as yet unresolved problems. In this present study, a novel NIR-reflected multispectral imaging system was developed for upconversion fluorescent imaging in small animals. Based on this system, we have obtained the high contrast images without the autofluorescence when biocompatible UCPs were injected near the body surface or deeply into the tissue. Furthermore, we have extracted respective spectra of the upconversion fluorescence and relatively quantify the fluorescence intensity with the multispectral analysis. To our knowledge, this is the first time to analyze and quantify the upconversion fluorescence in the small animal imaging.

Inhibitory Effect of Lactic Acid and Nisin on Bacterial Spoilage of Chilled Shrimp

Lactic acid alone and its combined application with nisin were evaluated for reducing population of naturally occurring microorganisms on chilled shrimp. Fresh shrimps were dipped in 0, 1.0% and 2.0% (v/v) lactic acid alone and their combined application with 0.04 (g/L/kg) nisin solution for 10 min. Total plate counts of aerobic bacteria (TPCs), Psychrotrophic counts, population of Pseudomonas spp., H2S producing bacteria and Lactic acid bacteria (LAB) on shrimps were determined during storage at 4 °C. The results indicated that total plate counts were 2.91 and 2.63 log CFU/g higher on untreated shrimps after 7 and 14 days of storage, respectively, than on shrimps treated with 2.0% lactic acid combined with 0.04 (g/L/kg) nisin. Both concentrations of lactic acid indicated significant reduction on Pseudomonas counts during storage, while 2.0% lactic acid combined with nisin indicated the highest reduction. In addition, H2S producing bacteria were more sensitive to high concentration of lactic acid combined with nisin during storage.

The Design of Self-evolving Artificial Immune System II for Permutation Flow-shop Problem

Artificial Immune System is adopted as a Heuristic Algorithm to solve the combinatorial problems for decades. Nevertheless, many of these applications took advantage of the benefit for applications but seldom proposed approaches for enhancing the efficiency. In this paper, we continue the previous research to develop a Self-evolving Artificial Immune System II via coordinating the T and B cell in Immune System and built a block-based artificial chromosome for speeding up the computation time and better performance for different complexities of problems. Through the design of Plasma cell and clonal selection which are relative the function of the Immune Response. The Immune Response will help the AIS have the global and local searching ability and preventing trapped in local optima. From the experimental result, the significant performance validates the SEAIS II is effective when solving the permutation flows-hop problems.

IFRS for SMEs: Challenge for Emerging Countries? Case of Czech Republic and Ukraine

Increase in globalization of capital markets brings the higher requirements on financial information provided for investors who look for a highly comparable information. Paper deals with the advantages and limitations of applying International Financial Reporting Standards (IFRS) in the Czech Republic and Ukraine. As a greatest limit for full adoption of IFRS shall be acknowledged the strong connection of continental accounting to tax system and enormous high administrative burden for IFRS appliers.

In vivo Antidiabetic and Antioxidant Potential of Pseudovaria macrophylla Extract

This study has investigated the antidiabetic and antioxidant potential of Pseudovaria macrophylla bark extract on streptozotocin–nicotinamide induced type 2 diabetic rats. LCMSQTOF and NMR experiments were done to determine the chemical composition in the methanolic bark extract. For in vivo experiments, the STZ (60 mg/kg/b.w, 15 min after 120 mg/kg/1 nicotinamide, i.p.) induced diabetic rats were treated with methanolic extract of Pseuduvaria macrophylla (200 and 400 mg/kg·bw) and glibenclamide (2.5 mg/kg) as positive control respectively. Biochemical parameters were assayed in the blood samples of all groups of rats. The pro-inflammatory cytokines, antioxidant status and plasma transforming growth factor βeta-1 (TGF-β1) were evaluated. The histological study of the pancreas was examined and its expression level of insulin was observed by immunohistochemistry. In addition, the expression of glucose transporters (GLUT 1, 2 and 4) were assessed in pancreas tissue by western blot analysis. The outcomes of the study displayed that the bark methanol extract of Pseuduvaria macrophylla has potentially normalized the elevated blood glucose levels and improved serum insulin and C-peptide levels with significant increase in the antioxidant enzyme, reduced glutathione (GSH) and decrease in the level of lipid peroxidation (LPO). Additionally, the extract has markedly decreased the levels of serum pro-inflammatory cytokines and transforming growth factor beta-1 (TGF-β1). Histopathology analysis demonstrated that Pseuduvaria macrophylla has the potential to protect the pancreas of diabetic rats against peroxidation damage by downregulating oxidative stress and elevated hyperglycaemia. Furthermore, the expression of insulin protein, GLUT-1, GLUT-2 and GLUT-4 in pancreatic cells was enhanced. The findings of this study support the anti-diabetic claims of Pseudovaria macrophylla bark.

e-Collaborative Learning Circles

In this paper, we introduce an e-collaborative learning circles methodology which utilizes the information and communication technologies (ICTs) in e-educational processes. In e-collaborative learning circles methodology, the teachers and students announce their research projects on various mailing lists and discussion boards using available ICTs. The teachers & moderators and students who are already members of the e-forums, discuss the project proposals in their classrooms sent out by the potential global partner schools and return the requested feed back to the proposing school(s) about their level of the participation and contribution in the research. In general, an e-collaborative learning circle project is implemented with a small and diverse group (usually 8-10 participants) from around the world. The students meet regularly over a period of weeks/months through the ICTs during the ecollaborative learning process. When the project is completed, a project product (e-book / DVD) is prepared and sent to the circle members. In this research, when taking into account the interests and motivation of the participating students with the facilitating role of the teacher(s), the students in each circle do research to obtain new data and information, thus enabling them to have the opportunity to meet both different cultures and international understandings across the globe. However, while the participants communicate along with the members in the circle they also practice and develop their communication language skills. Finally, teachers and students find the possibility to develop their skills in using the ICTs as well.

Dynamic Performances of Tubular Linear Induction Motor for Pneumatic Capsule Pipeline System

Tubular linear induction motor (TLIM) can be used as a capsule pump in a large pneumatic capsule pipeline (PCP) system. Parametric performance evaluation of the designed 1-meter diameter PCP-TLIM system yields encouraging results for practical implementation. The capsule thrust and speed inside the TLIM pump can be calculated from the combination of the PCP fluid mechanics and the TLIM equations. The TLIM equivalent circuits derived from those of the conventional three-phase induction motor are used as a model to predict the static test results of a small-scale PCP-TLIM system. In this paper, additional dynamic tests are performed on the same small-scale PCP-TLIM system with two capsules of different diameters. The behaviors of the capsule inside the pump are observed and analyzed. The dynamic performances from the dynamic tests are compared with the theoretical predictions based on the TLIM equivalent circuit model.

The Game of Synchronized Triomineering and Synchronized Tridomineering

In synchronized games players make their moves simultaneously rather than alternately. Synchronized Triomineering and Synchronized Tridomineering are respectively the synchronized versions of Triomineering and Tridomineering, two variants of a classic two-player combinatorial game called Domineering. Experimental results for small m × n boards (with m + n ≤ 12 for Synchronized Triomineering and m + n ≤ 10 for Synchronized Tridomineering) and some theoretical results for general k×n boards (with k = 3, 4, 5 for Synchronized Triomineering and k = 3 for Synchronized Tridomineering) are presented. Future research is indicated.

Efficiency of Compact Organic Rankine Cycle System with Rotary-Vane-Type Expander for Low-Temperature Waste Heat Recovery

This paper describes the experimental efficiency of a compact organic Rankine cycle (ORC) system with a compact rotary-vane-type expander. The compact ORC system can be used for power generation from low-temperature heat sources such as waste heat from various small-scale heat engines, fuel cells, electric devices, and solar thermal energy. The purpose of this study is to develop an ORC system with a low power output of less than 1 kW with a hot temperature source ranging from 60°C to 100°C and a cold temperature source ranging from 10°C to 30°C. The power output of the system is rather less due to limited heat efficiency. Therefore, the system should have an economically optimal efficiency. In order to realize such a system, an efficient and low-cost expander is indispensable. An experimental ORC system was developed using the rotary-vane-type expander which is one of possible candidates of the expander. The experimental results revealed the expander performance for various rotation speeds, expander efficiencies, and thermal efficiencies. Approximately 30 W of expander power output with 48% expander efficiency and 4% thermal efficiency with a temperature difference between the hot and cold sources of 80°C was achieved.

Influence of Surface-Treated Coarse Recycled Concrete Aggregate on Compressive Strength of Concrete

This paper reports on the influence of surface-treated coarse recycled concrete aggregate (RCA) on developing the compressive strength of concrete. The coarse RCA was initially treated by separately impregnating it in calcium metasilicate (CM) or wollastonite and nanosilica (NS) prepared at various concentrations. The effects of both treatment materials on concrete properties (e.g., slump, density and compressive strength) were evaluated. Scanning electron microscopy (SEM) analysis was performed to examine the microstructure of the resulting concrete. Results show that the effective use of treated coarse RCA significantly enhances the compressive strength of concrete. This result is supported by the SEM analysis, which indicates the formation of a dense interface between the treated coarse RCA and the cement matrix. Coarse RCA impregnated in CM solution results in better concrete strength than NS, and the optimum concentration of CM solution recommended for treated coarse RCA is 10%.

Fifth Order Variable Step Block Backward Differentiation Formulae for Solving Stiff ODEs

The implicit block methods based on the backward differentiation formulae (BDF) for the solution of stiff initial value problems (IVPs) using variable step size is derived. We construct a variable step size block methods which will store all the coefficients of the method with a simplified strategy in controlling the step size with the intention of optimizing the performance in terms of precision and computation time. The strategy involves constant, halving or increasing the step size by 1.9 times the previous step size. Decision of changing the step size is determined by the local truncation error (LTE). Numerical results are provided to support the enhancement of method applied.

Dynamic Variational Multiscale LES of Bluff Body Flows on Unstructured Grids

The effects of dynamic subgrid scale (SGS) models are investigated in variational multiscale (VMS) LES simulations of bluff body flows. The spatial discretization is based on a mixed finite element/finite volume formulation on unstructured grids. In the VMS approach used in this work, the separation between the largest and the smallest resolved scales is obtained through a variational projection operator and a finite volume cell agglomeration. The dynamic version of Smagorinsky and WALE SGS models are used to account for the effects of the unresolved scales. In the VMS approach, these effects are only modeled in the smallest resolved scales. The dynamic VMS-LES approach is applied to the simulation of the flow around a circular cylinder at Reynolds numbers 3900 and 20000 and to the flow around a square cylinder at Reynolds numbers 22000 and 175000. It is observed as in previous studies that the dynamic SGS procedure has a smaller impact on the results within the VMS approach than in LES. But improvements are demonstrated for important feature like recirculating part of the flow. The global prediction is improved for a small computational extra cost.

Improved IDR(s) Method for Gaining Very Accurate Solutions

The IDR(s) method based on an extended IDR theorem was proposed by Sonneveld and van Gijzen. The original IDR(s) method has excellent property compared with the conventional iterative methods in terms of efficiency and small amount of memory. IDR(s) method, however, has unexpected property that relative residual 2-norm stagnates at the level of less than 10-12. In this paper, an effective strategy for stagnation detection, stagnation avoidance using adaptively information of parameter s and improvement of convergence rate itself of IDR(s) method are proposed in order to gain high accuracy of the approximated solution of IDR(s) method. Through numerical experiments, effectiveness of adaptive tuning IDR(s) method is verified and demonstrated.

The Effects of Sodium Chloride in the Formation of Size and Shape of Gold (Au)Nanoparticles by Microwave-Polyol Method for Mercury Adsorption

Mercury is a natural occurring element and present in various concentrations in the environment. Due to its toxic effects, it is desirable to research mercury sensitive materials to adsorb mercury. This paper describes the preparation of Au nanoparticles for mercury adsorption by using a microwave (MW)-polyol method in the presence of three different Sodium Chloride (NaCl) concentrations (10, 20 and 30 mM). Mixtures of spherical, triangular, octahedral, decahedral particles and 1-D product were obtained using this rapid method. Sizes and shapes was found strongly depend on the concentrations of NaCl. Without NaCl concentration, spherical, triangular plates, octahedral, decahedral nanoparticles and 1D product were produced. At the lower NaCl concentration (10 mM), spherical, octahedral and decahedral nanoparticles were present, while spherical and decahedral nanoparticles were preferentially form by using 20 mM of NaCl concentration. Spherical, triangular plates, octahedral and decahedral nanoparticles were obtained at the highest NaCl concentration (30 mM). The amount of mercury adsorbed using 20 ppm mercury solution is the highest (67.5 %) for NaCl concentration of 30 mM. The high yield of polygonal particles will increase the mercury adsorption. In addition, the adsorption of mercury is also due to the sizes of the particles. The sizes of particles become smaller with increasing NaCl concentrations (size ranges, 5- 16 nm) than those synthesized without addition of NaCl (size ranges 11-32 nm). It is concluded that NaCl concentrations affects the formation of sizes and shapes of Au nanoparticles thus affects the mercury adsorption.

The Solar Wall in the Italian Climates

Passive systems were born with the purpose of the greatest exploitation of solar energy in cold climates and high altitudes. They spread themselves until the 80-s all over the world without any attention to the specific climate and the summer behavior; this caused the deactivation of the systems due to a series of problems connected to the summer overheating, the complex management and the rising of the dust. Until today the European regulation limits only the winter consumptions without any attention to the summer behavior but, the recent European EN 15251 underlines the relevance of the indoor comfort, and the necessity of the analytic studies validation by monitoring case studies. In the porpose paper we demonstrate that the solar wall is an efficient system both from thermal comfort and energy saving point of view and it is the most suitable for our temperate climates because it can be used as a passive cooling sistem too. In particular the paper present an experimental and numerical analisys carried out on a case study with nine different solar passive systems in Ancona, Italy. We carried out a detailed study of the lodging provided by the solar wall by the monitoring and the evaluation of the indoor conditions. Analyzing the monitored data, on the base of recognized models of comfort (ISO, ASHRAE, Givoni-s BBCC), is emerged that the solar wall has an optimal behavior in the middle seasons. In winter phase this passive system gives more advantages in terms of energy consumptions than the other systems, because it gives greater heat gain and therefore smaller consumptions. In summer, when outside air temperature return in the mean seasonal value, the indoor comfort is optimal thanks to an efficient transversal ventilation activated from the same wall.

Hybrid Energy Supply with Dominantly Renewable Option for Small Industrial Complex

The deficit of power for electricity demand reaches almost 30% for consumers in the last few years. This reflects with continually increasing the price of electricity, and today the price for small industry is almost 110Euro/MWh. The high price is additional problem for the owners in the economy crisis which is reflected with higher price of the goods. The paper gives analyses of the energy needs for real agro complex in Macedonia, private vinery with capacity of over 2 million liters in a year and with self grapes and fruits fields. The existing power supply is from grid with 10/04 kV transformer. The geographical and meteorological condition of the vinery location gives opportunity for including renewable as a power supply option for the vinery complex. After observation of the monthly energy needs for the vinery, the base scenario is the existing power supply from the distribution grid. The electricity bill in small industry has three factors: electricity in high and low tariffs in kWh and the power engaged for the technological process of production in kW. These three factors make the total electricity bill and it is over 110 Euro/MWh which is the price near competitive for renewable option. On the other side investments in renewable (especially photovoltaic (PV)) has tendency of decreasing with price of near 1,5 Euro/W. This means that renewable with PV can be real option for power supply for small industry capacities (under 500kW installed power). Therefore, the other scenarios give the option with PV and the last one includes wind option. The paper presents some scenarios for power supply of the vinery as the followings: • Base scenario of existing conventional power supply from the grid • Scenario with implementation of renewable of Photovoltaic • Scenario with implementation of renewable of Photovoltaic and Wind power The total power installed in a vinery is near 570 kW, but the maximum needs are around 250kW. At the end of the full paper some of the results from scenarios will be presented. The paper also includes the environmental impacts of the renewable scenarios, as well as financial needs for investments and revenues from renewable.

2D Spherical Spaces for Face Relighting under Harsh Illumination

In this paper, we propose a robust face relighting technique by using spherical space properties. The proposed method is done for reducing the illumination effects on face recognition. Given a single 2D face image, we relight the face object by extracting the nine spherical harmonic bases and the face spherical illumination coefficients. First, an internal training illumination database is generated by computing face albedo and face normal from 2D images under different lighting conditions. Based on the generated database, we analyze the target face pixels and compare them with the training bootstrap by using pre-generated tiles. In this work, practical real time processing speed and small image size were considered when designing the framework. In contrast to other works, our technique requires no 3D face models for the training process and takes a single 2D image as an input. Experimental results on publicly available databases show that the proposed technique works well under severe lighting conditions with significant improvements on the face recognition rates.

Identification of Factors Influencing Company's Competitiveness

Fast development of technologies, economic globalization and many other external circumstances stimulate company’s competitiveness. One of the major trends in today’s business is the shift to the exploitation of the Internet and electronic environment for entrepreneurial needs. Latest researches confirm that e-environment provides a range of possibilities and opportunities for companies, especially for micro-, small- and medium-sized companies, which have limited resources. The usage of e-tools raises the effectiveness and the profitability of an organization, as well as its competitiveness. In the electronic market, as in the classic one, there are factors, such as globalization, development of new technology, price sensitive consumers, Internet, new distribution and communication channels that influence entrepreneurship. As a result of eenvironment development, e-commerce and e-marketing grow as well. Objective of the paper: To describe and identify factors influencing company’s competitiveness in e-environment. Research methodology: The authors employ well-established quantitative and qualitative methods of research: grouping, analysis, statistics method, factor analysis in SPSS 20 environment, etc. The theoretical and methodological background of the research is formed by using scientific researches and publications, such as that from mass media and professional literature; statistical information from legal institutions as well as information collected by the authors during the surveying process. Research result: The authors detected and classified factors influencing competitiveness in e-environment.  In this paper, the authors presented their findings based on theoretical, scientific, and field research. Authors have conducted a research on e-environment utilization among Latvian enterprises. 

Image Retrieval: Techniques, Challenge, and Trend

This paper attempts to discuss the evolution of the retrieval techniques focusing on development, challenges and trends of the image retrieval. It highlights both the already addressed and outstanding issues. The explosive growth of image data leads to the need of research and development of Image Retrieval. However, Image retrieval researches are moving from keyword, to low level features and to semantic features. Drive towards semantic features is due to the problem of the keywords which can be very subjective and time consuming while low level features cannot always describe high level concepts in the users- mind.

Development of Rotational Smart Lighting Control System for Plant Factory

Rotational Smart Lighting Control System can supply the quantity of lighting which is required to run plants by rotating few LED and Fluorescent instead of that are used in the existing plant factories.The initial installation of the existing plants factory is expensive, so in order to solve the problem with smart lighting control system was developed. The beam required intensity for the growth of crops, Photosynthetic Photon Flux Density(PPFD)is calculated; and the number of LED, are installed on the blades, set; using the Lighting Simulation Program.Relux, it is able to confirm that the difference of the beam intensity between the center and the outer of lighting system when the lighting device is rotating.