Abstract: Doxorubicin (DOX) is an anthracycline drug used to treat many cancer diseases. Similarly to other cytostatic drugs, DOX has serious side effects; the biggest obstacle is the cardiotoxicity. With the aim of lowering the negative side effects and to target the DOX into the tumor tissue, the different nanoparticles (NPs) are studied. The aim of this work was to synthetized different NPs and conjugated them with DOX and determine the binding capacity of the NPs. For this experiment, carbon nanotubes (CNTs), graphene oxide (GO), fullerene (FUL) and liposomes (LIP) were used. The highest binding capacity was observed in GO (85%). Subsequently the toxicity of NPs and NPs-DOX conjugates was analyzed in in vivo system (chicken embryos). Some NPs (GO) can increase the toxicity of DOX, whereas other NPs (LIP, CNTs) decrease DOX toxicity.
Abstract: Stress of slaughter animals starting long before until at the time of process of slaughtering which cause misery and decrease of meat quality. Meanwhile, determination of animal stress using hormonal such as cortisol is expensive and less practical so that portable stress indicator for cows based on Fourier Transform Infrared Spectroscopy (FTIR) must be provided. The aims of this research are to find out the comparison process of slaughter between Rope Casting Local (RCL) and Restraining Box Method (RBM) by measuring of cortisol and wavelength in FTIR methods. Thirty two of male Ongole crossbred cattle were used in this experiment. Blood sampling was taken from jugular vein when they were rested and repeated when slaughtered. All of blood samples were centrifuged at 3000 rpm for 20 minutes to get serum, and then divided into two parts for cortisol assayed using ELISA and for measuring the wavelength using FTIR. The serum then measured at the wavelength between 4000-400 cm-1 using MB3000 FTIR. Band data absorption in wavelength of FTIR is analyzed descriptively by using FTIR Horizon MBTM. For RCL, average of serum cortisol when the animals rested were 11.47 ± 4.88 ng/mL, when the time of slaughter were 23.27 ± 7.84 ng/mL. For RBM, level of cortisol when rested animals were 13.67 ± 3.41 ng/mL and 53.47 ± 20.25 ng/mL during the slaughter. Based on student t-Test, there were significantly different between RBM and RCL methods when beef cattle were slaughtered (P0.05). Result of FTIR with the various of wavelength such as methyl group (=CH3 ) 2986cm-1, methylene (=CH2 ) 2827 cm-1, hydroxyl (- OH) 3371 cm-1, carbonyl (ketones) (C=O) 1636 cm-1, carboxyl (COO-1) 1408 cm-1, glucosa 1057 cm-1, urea 1011 cm-1have been obtained. It can be concluded that the RCL slaughtered method is better than the RBM method based on the increase of cortisol as an indicator of stress in beef cattle (P
Abstract: This paper presented a study of three algorithms, the
equalization algorithm to equalize the transmission channel with ZF
and MMSE criteria, application of channel Bran A, and adaptive
filtering algorithms LMS and RLS to estimate the parameters of the
equalizer filter, i.e. move to the channel estimation and therefore
reflect the temporal variations of the channel, and reduce the error in
the transmitted signal. So far the performance of the algorithm
equalizer with ZF and MMSE criteria both in the case without noise,
a comparison of performance of the LMS and RLS algorithm.
Abstract: Hybrid electric vehicles can reduce pollution and
improve fuel economy. Power-split hybrid electric vehicles (HEVs)
provide two power paths between the internal combustion engine
(ICE) and energy storage system (ESS) through the gears of an
electrically variable transmission (EVT). EVT allows ICE to operate
independently from vehicle speed all the time. Therefore, the ICE can
operate in the efficient region of its characteristic brake specific fuel
consumption (BSFC) map. The two-mode powertrain can operate in
input-split or compound-split EVT modes and in four different fixed
gear configurations. Power-split architecture is advantageous because
it combines conventional series and parallel power paths. This
research focuses on input-split and compound-split modes in the
two-mode power-split powertrain. Fuzzy Logic Control (FLC) for an
internal combustion engine (ICE) and PI control for electric machines
(EMs) are derived for the urban driving cycle simulation. These
control algorithms reduce vehicle fuel consumption and improve ICE
efficiency while maintaining the state of charge (SOC) of the energy
storage system in an efficient range.
Abstract: Presently a significant portion of the Earth's
population does not have access to healthy food. Either because they
cannot afford it or because they do not know which one are they. The
aim of the VII th Framework Chance project (Nr. 266331) supported
by the European Union has been to develop relatively cheap food
with favourable nutritional value and it should have acceptable
quality for consumers. As one task of the project we manufactured
bread products as a basic food. We examined the enrichment of bread
products with four kinds of bran, with a special milling product of
grain industry (aleurone-rich flour) and with a soy-based sprouted
additive. The applied concentration of the six mentioned additives
has been optimized and the physical properties of the bread products
were monitored. The weight/density of the enriched breads increased
a bit, however the volume and height decreased slightly compared to
the corresponding data of the control bread. The optimized
composition of the final product is favourably affected by these
additives having highly preferred composition from nutritional point
of view.
Abstract: In dynamic system theory a mathematical model is
often used to describe their properties. In order to find a transfer
matrix of a dynamic system we need to calculate an inverse matrix.
The paper contains the fusion of the classical theory and the
procedures used in the theory of automated control for calculating the
inverse matrix. The final part of the paper models the given problem
by the Matlab.
Abstract: Exploration and exploitation capabilities are both
important within Operations as means for improvement when
managed separately, and for establishing dynamic improvement
capabilities when combined in balance. However, it is unclear what
exploration and exploitation capabilities imply in improvement and
development work within an Operations context. So, in order to
better understand how to develop exploration and exploitation
capabilities within Operations, the main characteristics of these
constructs needs to be identified and further understood. Thus, the
objective of this research is to increase the understanding about
exploitation and exploration characteristics, to concretize what they
translates to within the context of improvement and development
work in an Operations unit, and to identify practical challenges. A
literature review and a case study are presented. In the literature
review, different interpretations of exploration and exploitation are
portrayed, key characteristics have been identified, and a deepened
understanding of exploration and exploitation characteristics is
described. The case in the study is an Operations unit, and the aim is
to explore to what extent and in what ways exploration and
exploitation activities are part of the improvement structures and
processes. The contribution includes an identification of key
characteristics of exploitation and exploration, as well as an
interpretation of the constructs. Further, some practical challenges are
identified. For instance, exploration activities tend to be given low
priority, both in daily work as in the manufacturing strategy. Also,
the overall understanding about the concepts of exploitation and
exploration (or any similar aspect of dynamic improvement
capabilities) is very low.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: Whey is the lactose rich by-product of the dairy
industry, having good amount of nutrient reservoir. Most abundant
nutrients are lactose, soluble proteins, lipids and mineral salts.
Disposing of whey by most of milk plants which do not have proper
pre-treatment system is the major issue. As a result of which, there
can be significant loss of potential food and energy source. Thus,
whey has been explored as the substrate for the synthesis of different
value added products such as enzymes. β-galactosidase is one of the
important enzymes and has become the major focus of research due
to its ability to catalyze both hydrolytic as well as
transgalactosylation reaction simultaneously. The enzyme is widely
used in dairy industry as it catalyzes the transformation of lactose to
glucose and galactose, making it suitable for the lactose intolerant
people. The enzyme is intracellular in both bacteria and yeast,
whereas for molds, it has an extracellular location. The present work
was carried to utilize the whey for the production of β-galactosidase
enzyme using both yeast and fungal cultures. The yeast isolate
Kluyveromyces marxianus WIG2 and various fungal strains have
been used in the present study. Different disruption techniques have
also been investigated for the extraction of the enzyme produced
intracellularly from yeast cells. Among the different methods tested
for the disruption of yeast cells, SDS-chloroform showed the
maximum β-galactosidase activity. In case of the tested fungal
cultures, Aureobasidium pullulans NCIM 1050 was observed to be
the maximum extracellular enzyme producer.
Abstract: This paper introduces a video sharing platform based
on WiFi, which consists of camera, mobile phone and PC server. This
platform can receive wireless signal from the camera and show the live
video on the mobile phone captured by camera. In addition, it is able to
send commands to camera and control the camera’s holder to rotate.
The platform can be applied to interactive teaching and dangerous
area’s monitoring and so on. Testing results show that the platform can
share the live video of mobile phone. Furthermore, if the system’s PC
server and the camera and many mobile phones are connected
together, it can transfer photos concurrently.
Abstract: The present study was aimed to examine the structure
of children’s adaptation during school transition and to identify a
commonality and dissimilarity at the elementary and junior high
school. 1,983 students in the 6th grade and 2,051 students in the 7th
grade were extracted by stratified two-stage random sampling and
completed the ASSESS that evaluated the school adaptation from the
view point of ‘general satisfaction’, ‘teachers’ support’, ‘friends’
support’, ‘anti-bullying relationship’, ‘prosocial skills’, and ‘academic
adaptation’. The 7th graders tend to be worse adaptation than the 6th
graders. A structural equation modeling showed the goodness of fit for
each grades. Both models were very similar but the 7th graders’ model
showed a lower coefficient at the pass from ‘teachers’ support’ to
‘friends’ support’. The role of ‘teachers’ support’ was decreased to
keep a good relation in junior high school. We also discussed how we
provide a continuous assistance for prevention of the 7th graders’ gap.
Abstract: Methicillin/multiple-resistant Staphylococcus aureus
(MRSA) are infectious bacteria that are resistant to common
antibiotics. A previous in silico study in our group has identified a
hypothetical protein SAV1226 as one of the potential drug targets. In
this study, we reported the bioinformatics characterization, as well as
cloning, expression, purification and kinetic assays of hypothetical
protein SAV1226 from methicillin/vancomycin-resistant
Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis
revealed a low degree of structural similarity with known proteins.
Kinetic assays demonstrated that hypothetical protein SAV1226 is
neither a domain of an ATP dependent dihydroxyacetone kinase nor
of a phosphotransferase system (PTS) dihydroxyacetone kinase,
suggesting that the function of hypothetical protein SAV1226 might
be misannotated on public databases such as UniProt and
InterProScan 5.
Abstract: Steady three-dimensional and two free surface waves
generated by moving bodies are presented, the flow problem to be
simulated is rich in complexity and poses many modeling challenges
because of the existence of breaking waves around the ship hull, and
because of the interaction of the two-phase flow with the turbulent
boundary layer. The results of several simulations are reported. The
first study was performed for NACA0012 of hydrofoil with different
meshes, this section is analyzed at h/c= 1, 0345 for 2D. In the second
simulation a mathematically defined Wigley hull form is used to
investigate the application of a commercial CFD code in prediction of
the total resistance and its components from tangential and normal
forces on the hull wetted surface. The computed resistance and wave
profiles are used to estimate the coefficient of the total resistance for
Wigley hull advancing in calm water under steady conditions. The
commercial CFD software FLUENT version 12 is used for the
computations in the present study. The calculated grid is established
using the code computer GAMBIT 2.3.26. The shear stress k-ωSST
model is used for turbulence modeling and the volume of fluid
technique is employed to simulate the free-surface motion. The
second order upwind scheme is used for discretizing the convection
terms in the momentum transport equations, the Modified HRIC
scheme for VOF discretization. The results obtained compare well
with the experimental data.
Abstract: The nanoindentation behaviour and phase
transformation of annealed single-crystal silicon wafers are examined.
The silicon specimens are annealed at temperatures of 250, 350 and
450ºC, respectively, for 15 minutes and are then indented to maximum
loads of 30, 50 and 70 mN. The phase changes induced in the indented
specimens are observed using transmission electron microscopy
(TEM) and micro-Raman scattering spectroscopy (RSS). For all
annealing temperatures, an elbow feature is observed in the unloading
curve following indentation to a maximum load of 30 mN. Under
higher loads of 50 mN and 70 mN, respectively, the elbow feature is
replaced by a pop-out event. The elbow feature reveals a complete
amorphous phase transformation within the indented zone, whereas
the pop-out event indicates the formation of Si XII and Si III phases.
The experimental results show that the formation of these crystalline
silicon phases increases with an increasing annealing temperature and
indentation load. The hardness and Young’s modulus both decrease as
the annealing temperature and indentation load are increased.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: The popularity of quality management system models
continues to grow despite the transitional crisis in 2008. Their
development is associated with demands of the new requirements for
entrepreneurs, such as risk analysis projects and more emphasis on
supervision of outsourced processes. In parallel, it is appropriate to
focus attention on the selection of companies aspiring to a quality
management system. This is particularly important in the automotive
supplier industry, where requirements transferred to the levels in the
supply chain should be clear, transparent and fairly satisfied. The
author has carried out a series of researches aimed at finding the
factors that allow for the effective implementation of the quality
management system in automotive companies. The research was
focused on four groups of companies: 1) manufacturing (parts and
assemblies for the purpose of sale or for vehicle manufacturers), 2)
service (repair and maintenance of the car) 3) services for the
transport of goods or people, 4) commercial (auto parts and vehicles).
The identified determinants were divided into two types of criteria:
internal and external, as well as hard and soft. The article presents the
hard – technical factors that an automotive company must meet in
order to achieve the goal of the quality management system
implementation.
Abstract: This article proposes a new method for application in
communication circuit systems that increase efficiency, PAE, output
power and gain in the circuit. The proposed method is based on a
combination of switching class-E and class-J and has been termed
class-EJ. This method was investigated using both theory and
simulation to confirm ∼72% PAE and output power of >39dBm. The
combination and design of the proposed power amplifier accrues gain
of over 15dB in the 2.9 to 3.5GHz frequency bandwidth. This circuit
was designed using MOSFET and high power transistors. The loadand
source-pull method achieved the best input and output networks
using lumped elements. The proposed technique was investigated for
fundamental and second harmonics having desirable amplitudes for
the output signal.
Abstract: A novel design technique employing CMOS Current
Feedback Operational Amplifier (CFOA) is presented. The feature of
consumption very low power in designing pseudo-OTA is used to
decreasing the total power consumption of the proposed CFOA. This
design approach applies pseudo-OTA as input stage cascaded with
buffer stage. Moreover, the DC input offset voltage and harmonic
distortion (HD) of the proposed CFOA are very low values compared
with the conventional CMOS CFOA due to the symmetrical input
stage. P-Spice simulation results are obtained using 0.18μm MIETEC
CMOS process parameters and supply voltage of ±1.2V, 50μA
biasing current. The p-spice simulation shows excellent improvement
of the proposed CFOA over existing CMOS CFOA. Some of these
performance parameters, for example, are DC gain of 62. dB, openloop
gain bandwidth product of 108 MHz, slew rate (SR+) of
+71.2V/μS, THD of -63dB and DC consumption power (PC) of
2mW.
Abstract: This paper discusses the forensic investigation of a
fatality-involved catastrophic structure collapse and the special
challenges faced when tasked with directing such an effort. While
this paper discusses the investigation’s findings and the outcome of
the event; this paper’s primary focus is on the challenges faced
directing a forensic investigation that requires coordinating with
governmental oversight while also having to accommodate multiple
parties’ investigative teams. In particular the challenges discussed
within this paper included maintaining on-site safety and operations
while accommodating outside investigator’s interests. In addition this
paper discusses unique challenges that one may face such as what to
do about unethical conduct of interested party’s investigative teams,
“off the record” sharing of information, and clandestinely transmitted
evidence.
Abstract: Development of new generation bio-tribological,
multilayer coatings opens an avenue for fabrication of future hightech
functional surfaces. In the presented work, nano-composite,
Cr/CrN+[Cr/ a-C:H implanted by metallic nanocrystals] multilayer
coatings have been developed for surface protection of medical tools.
Thin films were fabricated by a hybrid Pulsed Laser Deposition
technique. Complex microstructure analysis of nanomultilayer
coatings, subjected to mechanical and biological tests, were
performed by means of transmission electron microscopy (TEM).
Microstructure characterization revealed the layered arrangement of
Cr23C6 nanoparticles in multilayer structure. Influence of deposition
conditions on bio-tribological properties of the coatings was studied.
The bio-tests were used as a screening tool for the analyzed
nanomultilayer coatings before they could be deposited on medical
tools. Bio-medical tests were done using fibroblasts. The mechanical
properties of the coatings were investigated by means of a ball-ondisc
mechanical test. The micro hardness was done using Berkovich
indenter. The scratch adhesion test was done using Rockwell
indenter. From the bio-tribological point of view, the optimal
properties had the C106_1 material.