Abstract: Cryo-electron microscopy (CEM) in combination with
single particle analysis (SPA) is a widely used technique for
elucidating structural details of macromolecular assemblies at closeto-
atomic resolutions. However, development of automated software
for SPA processing is still vital since thousands to millions of
individual particle images need to be processed. Here, we present our
workflow for automated particle picking. Our approach integrates
peak shape analysis to the classical correlation and an iterative
approach to separate macromolecules and background by
classification. This particle selection workflow furthermore provides
a robust means for SPA with little user interaction. Processing
simulated and experimental data assesses performance of the
presented tools.
Abstract: Recently, permeable breakwaters have been suggested to overcome the disadvantages of fully protection breakwaters. These protection structures have minor impacts on the coastal environment and neighboring beaches where they provide a more economical protection from waves and currents. For regular waves, a numerical model is used (FLOW-3D, VOF) to investigate the hydraulic performance of a permeable breakwater. The model of permeable breakwater consists of a pair of identical vertical slotted walls with an impermeable upper and lower part, where the draft is a decimal multiple of the total depth. The middle part is permeable with a porosity of 50%. The second barrier is located at distant of 0.5 and 1.5 of the water depth from the first one. The numerical model is validated by comparisons with previous laboratory data and semi-analytical results of the same model. A good agreement between the numerical results and both laboratory data and semi-analytical results has been shown and the results indicate the applicability of the numerical model to reproduce most of the important features of the interaction. Through the numerical investigation, the friction factor of the model is carefully discussed.
Abstract: A new tool path planning method for 5-axis flank
milling of a globoidal indexing cam is developed in this paper. The
globoidal indexing cam is a practical transmission mechanism due
to its high transmission speed, accuracy and dynamic performance.
Machining the cam profile is a complex and precise task. The profile
surface of the globoidal cam is generated by the conjugate contact
motion of the roller. The generated complex profile surface is usually
machined by 5-axis point-milling method. The point-milling method
is time-consuming compared with flank milling. The tool path for
5-axis flank milling of globoidal cam is developed to improve the
cutting efficiency. The flank milling tool path is globally optimized
according to the minimum zone criterion, and high accuracy is
guaranteed. The computational example and cutting simulation finally
validate the developed method.
Abstract: Cabbage seedlings grown in vitro were exposed to
excess levels of heavy metals, including Cd, Mo, and Zn. High metal
levels affected plant growth at cotyledonary stage. Seedlings under
Cd, Mo, and Zn treatments could not produce root hairs and true
leaves. Under stress conditions, seedlings accumulated a higher
amount of anthocyanins in their cotyledons than those in the control.
The pigments isolated from Cd and Zn stressed seedling cotyledons
appeared as pink, while under Mo stress, was dark pink or purple.
Moreover, excess Mo stress increased antioxidant enzyme activities
of APX, CAT, SOD. These results suggest that, under excess Mo
stress, the induced antioxidant enzyme activity of cabbage seedlings
may function as a protective mechanism to shield the plants from
toxicity and exacerbated growth.
Abstract: This study examines the mediating effects of male
dyadic adjustment on the relationships between attachment and
attributional styles, and both psychological and physical husband
violence. Based on data from 68 married violent men recruited
through community organizations that work with violent men,
regression analyses showed that husbands- dyadic adjustment
mediates the associations between avoidant attachment and
attributional style, and psychological aggression, but not physical
violence. Scientific and clinical implications are discussed
Abstract: The effect of plant growth-promoting rhizobacteria
(PGPR) on seed germination, seedling growth and yield of field
grown maize were evaluated in three experiments. In these
experiments six bacterial strains include P.putida strain R-168,
P.fluorescens strain R-93, P.fluorescens DSM 50090, P.putida
DSM291, A.lipoferum DSM 1691, A.brasilense DSM 1690 were
used. Results of first study showed seed Inoculation significantly
enhanced seed germination and seedling vigour of maize. In second
experiment, leaf and shoot dry weight and also leaf surface area
significantly were increased by bacterial inoculation in both sterile
and non-sterile soil. The results showed that inoculation with
bacterial treatments had a more stimulating effect on growth and
development of plants in nonsterile than sterile soil. In the third
experiment, Inoculation of maize seeds with all bacterial strains
significantly increased plant height, 100 seed weight, number of seed
per ear and leaf area .The results also showed significant increase in
ear and shoot dry weight of maize.
Abstract: Protein-protein interactions (PPI) play a crucial role in many biological processes such as cell signalling, transcription, translation, replication, signal transduction, and drug targeting, etc. Structural information about protein-protein interaction is essential for understanding the molecular mechanisms of these processes. Structures of protein-protein complexes are still difficult to obtain by biophysical methods such as NMR and X-ray crystallography, and therefore protein-protein docking computation is considered an important approach for understanding protein-protein interactions. However, reliable prediction of the protein-protein complexes is still under way. In the past decades, several grid-based docking algorithms based on the Katchalski-Katzir scoring scheme were developed, e.g., FTDock, ZDOCK, HADDOCK, RosettaDock, HEX, etc. However, the success rate of protein-protein docking prediction is still far from ideal. In this work, we first propose a more practical measure for evaluating the success of protein-protein docking predictions,the rate of first success (RFS), which is similar to the concept of mean first passage time (MFPT). Accordingly, we have assessed the ZDOCK bound and unbound benchmarks 2.0 and 3.0. We also createda new benchmark set for protein-protein docking predictions, in which the complexes have experimentally determined binding affinity data. We performed free energy calculation based on the solution of non-linear Poisson-Boltzmann equation (nlPBE) to improve the binding mode prediction. We used the well-studied thebarnase-barstarsystem to validate the parameters for free energy calculations. Besides,thenlPBE-based free energy calculations were conducted for the badly predicted cases by ZDOCK and ZRANK. We found that direct molecular mechanics energetics cannot be used to discriminate the native binding pose from the decoys.Our results indicate that nlPBE-based calculations appeared to be one of the promising approaches for improving the success rate of binding pose predictions.
Abstract: The tensile properties of Mg-3%Al nanocrystalline
alloys were investigated at different test environment. Bulk
nanocrystalline samples of these alloy was successfully prepared by
mechanical alloying (MA) followed by cold compaction, sintering,
and hot extrusion process. The crystal size of the consolidated milled
sample was calculated by X-Ray line profile analysis. The
deformation mechanism and microstructural characteristic at
different test condition was discussed extensively. At room
temperature, relatively lower value of activation volume (AV) and
higher value of strain rate sensitivity (SRS) suggests that new rate
controlling mechanism accommodating plastic flow in the present
nanocrystalline sample. The deformation behavior and the
microstructural character of the present samples were discussed in
details.
Abstract: The paper presents a numerical investigation on the
rapid gas decompression in pure nitrogen which is made by using the
one-dimensional (1D) and three-dimensional (3D) mathematical
models of transient compressible non-isothermal fluid flow in pipes.
A 1D transient mathematical model of compressible thermal multicomponent
fluid mixture flow in pipes is presented. The set of the
mass, momentum and enthalpy conservation equations for gas phase
is solved in the model. Thermo-physical properties of multicomponent
gas mixture are calculated by solving the Equation of
State (EOS) model. The Soave-Redlich-Kwong (SRK-EOS) model is
chosen. This model is successfully validated on the experimental data
[1] and shows a good agreement with measurements. A 3D transient
mathematical model of compressible thermal single-component gas
flow in pipes, which is built by using the CFD Fluent code (ANSYS),
is presented in the paper. The set of unsteady Reynolds-averaged
conservation equations for gas phase is solved. Thermo-physical
properties of single-component gas are calculated by solving the Real
Gas Equation of State (EOS) model. The simplest case of gas
decompression in pure nitrogen is simulated using both 1D and 3D
models. The ability of both models to simulate the process of rapid
decompression with a high order of agreement with each other is
tested. Both, 1D and 3D numerical results show a good agreement
between each other. The numerical investigation shows that 3D CFD
model is very helpful in order to validate 1D simulation results if the
experimental data is absent or limited.
Abstract: In a 10-week (May – August, 2008) Phase I trial, 840, 1+ rainbow trout, Oncorhynchus mykiss, received a commercial oral immunomodulator, Fin Immune™, at four different dosages (0, 10, 20 and 30 mg g-1) to evaluate immune response and growth. The overall objective of was to determine an optimal dosage of this product for rainbow trout that provides enhanced immunity with maximal growth and health. Biweekly blood samples were taken from 10 randomly selected fish in each tank (30 samples per treatment) to evaluate the duration of enhanced immunity conferred by Fin-Immune™. The immunological assessment included serum white blood cell (lymphocyte, neutrophil) densities and blood hematocrit (packed cell volume %). Of these three variables, only lymphocyte density increased significantly among trout fed Fin- Immune™ at 20 and 30 mg g-1 which peaked at week 6. At week 7, all trout were switched to regular feed (lacking Fin-Immune™) and by week 10, lymphocyte levels decreased among all levels but were still greater than at week 0. There was growth impairment at the highest dose of Fin-Immune™ tested (30 mg g-1) which can be associated with a physiological compensatory mechanism due to a dose-specific threshold level. Thus, our main objective of this Phase I study was achieved, the 20 mg g-1 dose of Fin-Immune™ should be the most efficacious (of those we tested) to use for a Phase II disease challenge trial.
Abstract: This study was conducted to examine the effects of ketum extract on development of Chrysomya rufifacies and to analyze the presence of mitragynine in the larvae samples. 110 newly emerged first instar larvae of C. rufifacies were introduced on ketum extract-mixed cow liver at doses of 0, 20, 40 and 60g. Blowfly development rate was determined with 12 hour intervals and
mitragynine in larvae was extracted and quantitated. C. rufifacies in control group took about 192 hours to complete their development from first instar larvae to adult blowfly; meanwhile blowfly form from the highest dose of ketum was 264 hours. Mitragynine was detected in all groups of treatment, except for control. In conclusion, the presence of mitragynine in C. rufifacies is affected in delaying development rates of the blowfly for up to 62 hours or 3 days. Chemical analysis of mitragynine from larvae samples showed that this alkaloid present in all specimens analyzed.
Abstract: This paper focuses on systematic analysis and
controller design of the two-inertia STABILIZATION system,
considering the angular motion on a base body. This approach is
essential to the stabilization system to aim at a target under three or six
degrees of freedom base motion. Four controllers, such as
conventional PDF(Pseudo-Derivative Feedback) controller with
motor speed feedback, PDF controller with load speed feedback,
modified PDF controller with motor-load speed feedback and
feedforward controller added to modified PDF controller, are
suggested to improve reference tracking and disturbance rejection
performance. Characteristics and performance of each controller are
analyzed and validated by simulation in the case of the modified PDF
controller with and without a feedforward controller.
Abstract: This study attempts to validate the consumer-oriented
criteria list, developed by Wang et al. (2010), for selecting online
travel shopping sites. Based on a sample of 985 respondents,
confirmatory factor analysis was employed to test the factor structure
and assess the reliability and validity of the list. The results support the
list developed by Wang et al. (2010) and claim the list can be further
used to analyze, explain, and understand consumer behaviors about
online travel shopping.
Abstract: Server provisioning is one of the most attractive topics in virtualization systems. Virtualization is a method of running multiple independent virtual operating systems on a single physical computer. It is a way of maximizing physical resources to maximize the investment in hardware. Additionally, it can help to consolidate servers, improve hardware utilization and reduce the consumption of power and physical space in the data center. However, management of heterogeneous workloads, especially for resource utilization of the server, or so called provisioning becomes a challenge. In this paper, a new concept for managing workloads based on user behavior is presented. The experimental results show that user behaviors are different in each type of service workload and time. Understanding user behaviors may improve the efficiency of management in provisioning concept. This preliminary study may be an approach to improve management of data centers running heterogeneous workloads for provisioning in virtualization system.
Abstract: Bio-electrical responses obtained from freshwater
sediments by employing microbial fuel cell (MFC) technology were
investigated in this experimental study. During the electricity
generation, organic matter in the sediment was microbially oxidized
under anaerobic conditions with an electrode serving as a terminal
electron acceptor. It was found that the sediment organic matter
(SOM) associated with electrochemically-active electrodes became
more humified, aromatic, and polydispersed, and had a higher average
molecular weight, together with the decrease in the quantity of SOM.
The alteration of characteristics of the SOM was analogous to that
commonly observed in the early stage of SOM diagenetic process (i.e.,
humification). These findings including an elevation of the sediment
redox potential present a possibility of the MFC technology as a new
soil/sediment remediation technique based on its potential benefits:
non-destructive electricity generation and bioremediation.
Abstract: Palm methyl ester (PME) is one of the alternative
biomass fuels to liquid fossil fuels. To investigate the combustion
characteristics of PME as an alternative fuel for gas turbines, combustion experiments using two types of burners under atmospheric
pressure were performed. One of the burners has a configuration
making strong non-premixed flame, whereas the other has a
configuration promoting prevaporization of fuel droplets. The results
show that the NOx emissions can be reduced by employing the latter burner without accumulation of soot when PME is used as a fuel. A
burner configuration promoting prevaporzation of fuel droplets is
recommended for PME.
Abstract: Social-economic variables influence transportation
demand largely. Analyses of discrete choice model consider
social-economic variables to study traveler-s mode choice and
demand. However, to calibrate the discrete choice model needs to have
plenty of questionnaire survey. Also, an aggregative model is
proposed. The historical data of passenger volumes for high speed rail
and domestic civil aviation are employed to calibrate and validate the
model. In this study, models with different social-economic variables,
which are oil price, GDP per capita, CPI and economic growth rate,
are compared. From the results, the model with the oil price is better
than models with the other social-economic variables.
Abstract: Inflammatory bowel disease (IBD) is a chronic
relapsing-remitting condition that afflicts millions of people
throughout the world and impairs their daily functions and quality of
life. Treatment of IBD depends largely on 5-aminosalicylic acid (5-
ASA) and corticosteroids. The present study aimed to clarify the
effects of 5-aminosalicylic acid, budesonide and currcumin on 90
male albino rats against trinitrobenzene sulfonic acid (TNB) induced
colitis. TNB was injected intrarectally to 50 rats. The other 40 rats
served as control groups. Both 5-ASA (in a dose of 120 mg/kg) and
budesonide (in a dose of 0.1 mg/kg) were administered daily for one
week whereas currcumin was injected intraperitonially (in a dose of
30 mg/kg daily) for 14 days after injection of either TNB in the
colitis rats (group B) or saline in control groups (group A). The study
included estimation of macroscopic score index, histological
examination of H&E stained sections of the colonic tissue,
biochemical estimation of myeloperoxidase (MPO), nitric oxide
(NO), and caspase-3 levels, in addition to studying the effect of tested
drugs on colonic motility. It was found that budesonide and curcumin
improved mucosal healing, reduced both NO production and caspase-
3 level. They had the best impact on the disturbed colonic motility in
TNBS-model of colitis.
Abstract: Homogeneous composites of alumina and zirconia
with a small amount of MgO (99%) were obtained for ZTA ceramic containing 0.05 wt% MgO in
1500 °C.
Abstract: Viral influenza A subtypes H5N1 and pandemic
H1N1 (pH1N1) have worldwide emerged and transmitted. The most
common anti-influenza drug for treatment of both seasonal and
pandemic influenza viruses is oseltamivir that nowadays becomes
resistance to influenza neuraminidase. The novel long-acting drug,
laninamivir, was discovered for treatment of the patients infected
with influenza B and influenza A viruses. In the present study,
laninamivir complexed with wild-type strain of both H5N1 and
pH1N1 viruses were comparatively determined the structures and
drug-target interactions by means of molecular dynamics (MD)
simulations. The results show that the hydrogen bonding interactions
formed between laninamivir and its binding residues are likely
similar for the two systems. Additionally, the presence of
intermolecular interactions from laninamivir to the residues in the
binding pocket is established through their side chains in accordance
with hydrogen bond interactions.