Abstract: The cultivation of black glutinous rice rich in anthocyanins can provide great benefits to both farmers and consumers. Total anthocyanins content and yield component data of black glutinous rice cultivar (KHHK) grown with the addition of mineral elements (Ca, Mg, Cu, Cr, Fe and Se) under soilless conditions were studied. Ca application increased seed anthocyanins content by three-folds compared to controls. Cu application to rice plants obtained the highest number of grains panicle, panicle length and subsequently high panicle weight. Se application had the largest effect on leaf anthocyanins content, the number of tillers, number of panicles and 100-grain weight. These findings showed that the addition of mineral elements had a positive effect on increasing anthocyanins content in black rice plants and seeds as well as the heightened development of black glutinous rice plant growth.
Abstract: Gamma irradiation greatly reduces the potential microbiological risk of fresh fruits, resulting in improved microbial safety as well as extending their shelf life. The effects of 0.5-2 kGy gamma doses on some physicochemical, microbial and sensory properties of fresh barberry fruits (Berberis vulgaris) during refrigerated storage for 40 days were evaluated. The total anthocyanin and total phenolic contents of barberry fruits decreased in a dose-dependent manner immediately after irradiation and after subsequent storage. In general, it is recommended that, according to the effect of gamma radiation on physicochemical, microbial and sensorial characteristics, doses of 1.25-2 kGy could be used.
Abstract: Natural dyes, extracted from black carrot and bramble, were utilized as photosensitizers to prepare dye-sensitized solar cells (DSSCs). Spectrophotometric studies of the natural dyes in solution and on a titanium dioxide substrate were carried out in order to assess changes in the status of the dyes. The results show that the bathochromic shift is seen on the photo-electrode substrate. The chemical binding of the natural dyes at the surface photo-electrode were increased by the chelating effect of the Ti(IV) ions. The cyclic voltammetry results showed that all extracts are suitable to be performed in DSSCs. Finally, photochemical performance and stability of DSSCs based on natural dyes were studied. The DSSCs sensitized by black carrot extract have been reported to achieve up to Jsc=1.17 mAcm-2, Voc= 0.55 V, FF= 0.52, η=0.34%, whereas Bramble extract can obtain up to Jsc=2.24 mAcm-2, Voc= 0.54 V, FF= 0.57, η=0.71%. The power conversion efficiency was obtained from the mixed dyes in DSSCs. The power conversion efficiency of dye-sensitized solar cells using mixed Black carrot and Bramble dye is the average of the their efficiency in single DSSCs.
Abstract: In recent years, tendency to use of natural antimicrobial agents in food industry has increased. Pomegranate peels containing phenolic compounds and anti-microbial agents, are counted as valuable source for extraction of these compounds. In this study, the extraction of pomegranate peel extract was carried out at different ethanol/water ratios (40:60, 60:40, and 80:20), temperatures (25, 40, and 55 ˚C), and time durations (20, 24, and 28 h). The extraction yield, phenolic compounds, flavonoids, and anthocyanins were measured. Antimicrobial activity of pomegranate peel extracts were determined against some food-borne microorganisms such as Salmonella enteritidis, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, Aspergillus niger, and Saccharomyces cerevisiae by agar diffusion and MIC methods. Results showed that at ethanol/water ratio 60:40, 25 ˚C and 24 h maximum amount of phenolic compounds (349.518 mg gallic acid/g dried extract), flavonoids (250.124 mg rutin/g dried extract), anthocyanins (252.047 mg cyanidin3glucoside/100 g dried extract), and the strongest antimicrobial activity were obtained. All extracts’ antimicrobial activities were demonstrated against every tested microorganisms. Staphylococcus aureus showed the highest sensitivity among the tested microorganisms.
Abstract: Broccoli is considered as being a rich source of AOX like flavonoids, polyphenols, anthocyanins etc. and of major interest especially in the organic sector. However, AOX is environment dependent and often varies between cultivars. Aim of the study was to investigate the impact of cultivar and harvest date on AOX in broccoli. Activity of the AOX was determined using a Photochem®-Analyzer and a kit of reagent solutions for analysis. Results of the study showed that the lipid (ACL) and water-soluble antioxidant potential (AWC) of broccoli heads varied significantly between the four harvesting dates, but not among the different cultivars. The highest concentration of ACL was measured in broccoli heads harvested in September 2011, followed by heads harvested at the beginning of July in 2012. ACW was highest in heads harvested in October 2011. Lowest concentrations of ACW were measured in heads harvested in June 2012. Overall, the study indicated that the harvest date and thus growing conditions seem to be of high importance for final antioxidant capacity of broccoli.
Abstract: Because blueberries are worldwide recognized as a
good source of beneficial components, their consumption has
increased in the past decades, and so have the scientific works about
their properties. Hence, this work was undertaken to evaluate the
effect of some production and conservation factors on the properties
of blueberries from cultivar Bluecrop. The physical and chemical
analyses were done according to established methodologies and then
all data was treated using software SPSS for assessment of the
possible differences among the factors investigated and/or the
correlations between the variables at study. The results showed that
location of production influenced some of the berries properties
(caliber, sugars, antioxidant activity, color and texture) and that the
age of the bushes was correlated with moisture, sugars and acidity, as
well as lightness. On the other hand, altitude of the farm only was
correlated to sugar content. With regards to conservation, it
influenced only anthocyanins content and DPPH antioxidant activity.
Finally, the type of extract and the order of extraction had a
pronounced influence on all the phenolic properties evaluated.
Abstract: Mangosteen (Garcinia mangostana) pericarp is
considered as agricultural waste and not fully utilized in food
products. It is widely reported that mangosteen pericarp contains high
antioxidant properties. The objective of this study is to develop novel
yellow alkaline noodle (YAN) substituted with different levels of
mangosteen pericarp powder (MPP). YAN formulation was
substituted with different levels of MPP (0%, 5%, 10% and 15%).
The effect on nutritional and antioxidant properties was evaluated.
Higher substitution levels of MPP resulted in significant increase
(p
Abstract: Blueberries are widely valued for their high content in
phenolic compounds with antioxidant activity, and hence beneficial
for the human health. In this way, a study was done to determine the
phenolic composition (total phenols, anthocyanins and tannins) and
antioxidant activity of blueberries from three cultivars (Duke,
Bluecrop, and Ozarkblue) grown in two different Portuguese farms.
Initially two successive extractions were done with methanol
followed by two extractions with aqueous acetone solutions. These
extracts obtained were then used to evaluate the amount of phenolic
compounds and the antioxidant activity. The total phenols were
observed to vary from 4.9 to 8.2 mg GAE/g fresh weight, with
anthocyanin’s contents in the range 1.5-2.8 mg EMv3G/g and tannins
contents in the range 1.5- 3.8 mg/g. The results for antioxidant
activity ranged from 9.3 to 23.2 molTE/g and from 24.7 to 53.4molTE/g, when measured, respectively, by DPPH and ABTS
methods. In conclusion it was observed that, in general, the cultivar
had a visible effect on the phenols present, and furthermore, the
geographical origin showed relevance either in the phenols contents
or the antioxidant activity.
Abstract: Complexation of anthocyanins to mimic natural
copigmentation process was investigated. Cyanidin-rich extracts from
Zea mays L. ceritina Kulesh. and delphinidin-rich extracts from
Clitoria ternatea L. were used to form 4 anthocyanin complexes,
AC1, AC2, AC3 and AC4, in the presence of several polyphenols and
a trace metal. Characterizations of the ACs were conducted by UV,
FTIR, DSC/TGA and morphological observations. Bathochromic
shifts of the UV spectra of 4 formulas of ACs were observed at peak
wavelengths of about 510-620 nm by 10 nm suggesting complex
formation. FTIR spectra of the ACs indicate shifts of peaks from
1,733 cm-1 to 1,696 cm-1 indicating interactions and a decrease in the
peak areas within the wavenumber of 3,400-3,500 cm-1 indicating
changes in hydrogen bonding. Thermal analysis of all of the ACs
suggests increases in melting temperature after complexation. AC
with the highest melting temperature was morphologically observed
by SEM and TEM to be crystal-like particles within a range of 50 to
200 nm. Particle size analysis of the AC by laser diffraction gave a
range of 50-600 nm, indicating aggregation. This AC was shown to
have no cytotoxic effect on cultured HGEPp0.5 and HGF (all p>
0.05) by MTT. Therefore, complexation of anthocyanins was simple
and self-assembly process, potentially resulting in nanosized particles
of anthocyanin complex.
Abstract: Anthocyanins are natural pigments with effective UV
protection but their topical use could be limited due to their
physicochemical characteristics. An attempt to overcome such
limitations by complexation of 2 major anthocyanin-rich sources, C.
ternatea and Z. mays, has potentiated its use as topical antiinflammatory.
Cell studies indicate no cytotoxicity of the
anthocyanin complex (AC) up to 1 mg/ml tested in HaCaT and
human fore head fibroblasts by MTT. Croton oil-induced ear edema
in Wistar rats suggests an effective dose of 5 mg/cm2 of AC as a
topical anti-inflammatory in comparison to 0.5 mg/cm2 of
fluocinolone acetonide. Niosomal encapsulation of the AC
significantly prolonged the anti-inflammatory activity particularly at
8 h after topical application (p = 0.0001). The AC was not cytotoxic
and its anti-inflammatory and activity was dose-dependent and
prolonged by niosomal encapsulation. It has also shown to promote
collagen type 1 production in cell culture. Thus, AC could be a
potential candidate for topical anti-inflammatory agent from natural
resources.
Abstract: Utilizing solar energy in producing electricity can minimize environmental pollution generated by fossil fuel in producing electricity. Our research was base on the extraction of dye from Roystonea regia fruit by using methanol as solvent. The dye extracts were used as sensitizers in Dye-sensitized solar cell (DSSCs). Study was done on the electrical properties from the extracts of Roystonea regia fruit as Dye-sensitized solar cell (DSSCs). The absorptions of the extracts and extracts with dye were determined at different wavelengths (350-1000nm). Absorption peak was observed at 1.339 at wavelength 400nm. The obtained values for methanol extract Roystonea regia extract are, Imp = 0.015mA, Vmp = 12.0mV, fill factor = 0.763, Isc= 0.018 mA and Voc = 13.1 mV and efficiency of 0.32%. .The phytochemical screening was taken and it was observed that Roystonea regia extract contained less of anthocyanin compared to flavonoids. The nanostructured dye sensitized solar cell (DSSC) will provide economically credible alternative to present day silicon p–n junction photovoltaic.
Abstract: This study was conducted to evaluate the response of almond genotypes to osmotic stress in vitro in order to screen drought tolerance. Explants subjected to polyethyleneglycol osmotic stress (0, 3.5, and 7.0% WV) on the MS medium. Concentrations of photosynthesis pigments, anthocyanins, and carothenoids were significantly reduced under osmotic stress. Under osmotic stress, leaf water content, cellular membrane stability and pigments concentrations were significantly higher in the leaves of drought tolerant genotypes. The results revealed that carotenoids and anthocyanins may act as photoprotectant compounds in almond leaves and involved in drought tolerance system of the plant.
Abstract: Cabbage seedlings grown in vitro were exposed to
excess levels of heavy metals, including Cd, Mo, and Zn. High metal
levels affected plant growth at cotyledonary stage. Seedlings under
Cd, Mo, and Zn treatments could not produce root hairs and true
leaves. Under stress conditions, seedlings accumulated a higher
amount of anthocyanins in their cotyledons than those in the control.
The pigments isolated from Cd and Zn stressed seedling cotyledons
appeared as pink, while under Mo stress, was dark pink or purple.
Moreover, excess Mo stress increased antioxidant enzyme activities
of APX, CAT, SOD. These results suggest that, under excess Mo
stress, the induced antioxidant enzyme activity of cabbage seedlings
may function as a protective mechanism to shield the plants from
toxicity and exacerbated growth.
Abstract: The strawberry jam is rich in bioactive compounds. It
is economically and commercially important and widely consumed.
Different strawberries cultivars can be used for its preparation,
however, a careful selection should be performed to guarantee the
preservation of bioactive compounds during jam storage. Two
strawberry cultivars (Camarosa and American 13) were analyzed by
HPLC, three anthocyanins: cyanidin-3-glucoside, pelargonidin-3-
glucoside and pelargonidin-3-rutinoside were quantified. Camarosa
strawberries presented significantly higher concentration of
anthocyanins (p
Abstract: Many of the compounds present in potato are important because of their beneficial effects on health, therefore, are highly desirable in the human diet. Potato tubers contain significant amounts of anthocyanins. The aim of this research was to determine the content of anthocyanins and its relationship with the colour of organically and conventionally cultivated potato varieties. In the research eight potato samples of three potato varieties were analyzed on anthocyanins, dry matter content and color. Obtained results show that there was no significant influence on amount of anthocyanins between different cultivation environments (p>0.05) while between varieties – significant difference (p
Abstract: To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.
Abstract: The agricultural organic farming is different from
conventional farming in a way that is aimed at providing a balanced
and constructive action in agricultural systems. With the increase in
intensive agriculture, undesirable changes were being observed in
ecosystems with irreparable damage being caused to the natural
equilibrium. This is the reason for the increasing interest in organic
farming as an environment friendly agricultural production method.
In the present work three red fruits produced in organic farming were
analyzed, namely raspberry, gooseberry and blueberry. The samples
were harvested in a local farm when at plain maturation. The results
obtained allowed to conclude that the blueberry contained higher
amounts of phenolic compounds, total tannins and total anthocyanins
than raspberry and gooseberry. Furthermore, the HPLC analysis
allowed to identify monomeric anthocyanins and phenolic acids in
the three fruits studied.
Abstract: Sweet cherries (Prunus avium L.) contain various
phenolic compounds which contribute to total antioxidant activity.
Total polyphenols, tannins, flavonoids and anthocyanins, and
antioxidant capacity in a fruits of a number of selected sweet cherry
genotypes were investigated. Total polyphenols content ranged from
4.12 to 8.34 mg gallic acid equivantents/g dry fruit weight and total
tannins content ranged from 0.19 to 1.95 mg gallic acid equivalent/g
dry fruit weight. Total flavonoids were within the range 0.42-1.56 mg
of rutin equivalents/g dry fruit weight and total anthocyanins content
were between 0.35 and 0.69 mg cyanidin 3-glucoside equivalent/ g
dry fruit weight. Although sweet cherry fruits are a significant source
of different phenolic compounds, antioxidant activity of sweet
cherries is not related only with the total polyphenolics, flavonoids or
anthocyanins.
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.