Abstract: Austenite and Martensite indicate the phases of solids undergoing phase transformation which we usually associate with materials and not with living organisms. This article provides an overview of bacterial proteins and structures that are undergoing phase transformation and suggests its probable effect on mechanical behavior. The context is mainly within the role of phase transformations occurring in the flagellum of bacteria. The current knowledge of molecular mechanism leading to phase variation in living organisms is reviewed. Since in bacteria, each flagellum is driven by a separate motor, similarity to a Differential drive in case of four-wheeled vehicles is suggested. It also suggests the application of the mechanism in which bacteria changes its direction of movement to facilitate single point turning of a multi-wheeled vehicle. Finally, examples are presented to illustrate that the motion due to phase transformation of flagella in bacteria can start a whole new research on motion mechanisms.
Abstract: To study on effect of PEG and NaCl stress on
germination and early seedling stages on two cultivar of corn, two
separated experiment were laid out at physiology laboratory, faculty
of Agriculture, Razi University, Kermanshah, Iran in 2009. This
investigation was performed as factorial experiment under Complete
Randomized Design (CRD) with three replications. Cultivar factor
contains of two varieties (sweet corn SC403 and Flint corn SC704)
and five levels of stress (0, -2, -4, -6 and -8 bar). The principal aim of
current study was to compare the two varieties of maize in relative to
the stress conditions. Results indicated that significant decrease was
observed in percentage of germination, germination rate, length of
radicle and plumule and radicle and plumule dry matter. On the basis
of the results, NaCl as compared with PEG had more effect on
germination and early seedling stage and sweet corn had more
resistant than flint corn in both stress conditions.
Abstract: Vermicomposting is the conversion of organic waste
into bio-fertilizers through the action of earthworm. This technology
is widely used for organic solid waste management. Waste corn pulp
blended with cow dung manure was vermicomposted over 30 days
using Eisenia fetida earthworms species. pH, temperature, moisture
content, and electrical conductivity were daily monitored. The
feedstock, vermicompost and vermiwash were analyzed for nutrient
composition. The average temperature and moisture content in the
vermi-reactor was 22.5°C and 42.5% respectively. The vermicompost
and vermiwash had an almost neutral pH whilst the electrical
conductivity was 21% higher in the vermicompost. The nitrogen and
potassium content was 57% and 79.6% richer in the vermicompost
respectively compared to the vermiwash. However, the vermiwash
was 84% richer in phosphorous as compared to vermicompost.
Furthermore, the vermiwash was 89.1% and 97.6% richer in Ca and
Mg respectively and was 97.8% richer in Na salts compared to the
vermicompost. The vermiwash also indicated a significantly higher
amount of micronutrients. Both bio-fertilizers were rich in nutrients
specification for fertilizers.
Abstract: Malting is usually carried out on intact barley seed,
while hull is still attached to it. In this study, oat grain with and
without hull was subjected to controlled germination to optimize its
enzymes activity, in such a way that lipase has the lowest and α-
amylase and proteinase the highest activities. Since pH has a great
impact on the activity of the enzymes, the pH of germination media
was set up to 3 to 8. In dehulled oats, lipase and α-amylase had the
lowest and highest activities in pHs 3 and 6, respectively whereas the
highest proteinase activity was evidenced at pH 7 and 4 in the oats
with and without hull respectively. While measurements indicated
that the effect of hull on the enzyme activities particularly in lipase
and amylase at each level of the pH are significantly different, the
best results were obtained in those samples in which their hull had
been removed. However, since the similar lipase activity in
germinated dehulled oat were recorded at the pHs 4 and 5, therefore
it was concluded that pH 5 in dehulled oat seed may provide the
optimum enzyme activity for all the enzymes.
Abstract: Discovering new biological knowledge from the highthroughput biological data is a major challenge to bioinformatics today. To address this challenge, we developed a new approach for protein classification. Proteins that are evolutionarily- and thereby functionally- related are said to belong to the same classification. Identifying protein classification is of fundamental importance to document the diversity of the known protein universe. It also provides a means to determine the functional roles of newly discovered protein sequences. Our goal is to predict the functional classification of novel protein sequences based on a set of features extracted from each protein sequence. The proposed technique used datasets extracted from the Structural Classification of Proteins (SCOP) database. A set of spectral domain features based on Fast Fourier Transform (FFT) is used. The proposed classifier uses multilayer back propagation (MLBP) neural network for protein classification. The maximum classification accuracy is about 91% when applying the classifier to the full four levels of the SCOP database. However, it reaches a maximum of 96% when limiting the classification to the family level. The classification results reveal that spectral domain contains information that can be used for classification with high accuracy. In addition, the results emphasize that sequence similarity measures are of great importance especially at the family level.
Abstract: Maize and Indian mustard are significant crops in
semi-arid climate zones of India. Improved water management
requires precise scheduling of irrigation, which in turn requires an
accurate computation of daily crop evapotranspiration (ETc). Daily
crop evapotranspiration comes as a product of reference
evapotranspiration (ET0) and the growth stage specific crop
coefficients modified for daily variation. The first objective of
present study is to develop crop coefficients Kc for Maize and Indian
mustard. The estimated values of Kc for maize at the four crop
growth stages (initial, development, mid-season, and late season) are
0.55, 1.08, 1.25, and 0.75, respectively, and for Indian mustard the Kc
values at the four growth stages are 0.3, 0.6, 1.12, and 0.35,
respectively. The second objective of the study is to compute daily
crop evapotranspiration from ET0 and crop coefficients. Average
daily ETc of maize varied from about 2.5 mm/d in the early growing
period to > 6.5 mm/d at mid season. The peak ETc of maize is 8.3
mm/d and it occurred 64 days after sowing at the reproductive growth
stage when leaf area index was 4.54. In the case of Indian mustard,
average ETc is 1 mm/d at the initial stage, >1.8 mm/d at mid season
and achieves a peak value of 2.12 mm/d on 56 days after sowing.
Improved schedules of irrigation have been simulated based on daily
crop evapo-transpiration and field measured data. Simulation shows a
close match between modeled and field moisture status prevalent
during crop season.
Abstract: The approach proposed here is oriented in the direction of fuzzy system for the analysis and the synthesis of intelligent climate controllers, the simulation of the internal climate of the greenhouse is achieved by a linear model whose coefficients are obtained by identification. The use of fuzzy logic controllers for the regulation of climate variables represents a powerful way to minimize the energy cost. Strategies of reduction and optimization are adopted to facilitate the tuning and to reduce the complexity of the controller.
Abstract: The experiment was then conducted to investigate the
effect of cassava peel addition in the concentrate on the performance
of lactating dairy cows. Twenty four Holstein Friesian crossbred
(>87.5% Holstein Friesian) lactating dairy cows in mid lactation;
averaging 12.2+2.1 kg of milk, 119+45 days in milk, 44.1+6.2
months old and 449+33 kg live weight, were stratified for milk yield,
days in milk, age, stage of lactation and body weight, and then
randomly allocated to three treatment groups. The first, second and
third groups were fed concentrates containing the respective cassava
peel, 0, 20 and 40%. All cows were fed ad libitum corn silage and
freely access to clean water. Dry matter intake, 4%FCM, milk
composition and body weight change were affected (P
Abstract: Biological reactions of individuals of a testing animal
to toxic substance are unique and can be used as an indication of the
existing of toxic substance. However, to distinguish such phenomenon
need a very complicate system and even more complicate to analyze
data in 3 dimensional. In this paper, a system to evaluate in vitro
biological activities to acute toxicity of stochastic self-affine
non-stationary signal of 3D goldfish swimming by using fractal
analysis is introduced. Regular digital camcorders are utilized by
proposed algorithm 3DCCPC to effectively capture and construct 3D
movements of the fish. A Critical Exponent Method (CEM) has been
adopted as a fractal estimator. The hypothesis was that the swimming
of goldfish to acute toxic would show the fractal property which
related to the toxic concentration. The experimental results supported
the hypothesis by showing that the swimming of goldfish under the
different toxic concentration has fractal properties. It also shows that
the fractal dimension of the swimming related to the pH value of FD Ôëê
0.26pH + 0.05. With the proposed system, the fish is allowed to swim
freely in all direction to react to the toxic. In addition, the trajectories
are precisely evaluated by fractal analysis with critical exponent
method and hence the results exhibit with much higher degree of
confidence.
Abstract: The use of magnetic and magnetic/gold core/shell
nanoparticles in biotechnology or medicine has shown good promise
due to their hybrid nature which possesses superior magnetic and
optical properties. Some of these potential applications include
hyperthermia treatment, bio-separations, diagnostics, drug delivery
and toxin removal. Synthesis refinement to control geometric and
magnetic/optical properties, and finding functional surfactants for
biomolecular attachment, are requirements to meet application
specifics.
Various high-temperature preparative methods were used for the
synthesis of iron oxide and gold-coated iron oxide nanoparticles.
Different surface functionalities, such as 11-aminoundecanoic and
11-mercaptoundecanoic acid, were introduced on the surface of the
particles to facilitate further attachment of biomolecular functionality
and drug-like molecules. Nanoparticle thermal stability, composition,
state of aggregation, size and morphology were investigated and the
results from techniques such as Fourier Transform-Infra Red
spectroscopy (FT-IR), Ultraviolet visible spectroscopy (UV-vis),
Transmission Electron Microscopy (TEM) and thermal analysis are
discussed.
Abstract: Changes in stem diameter of orchid plants were
investigated in a control growing climate. Previous studies have
focused on stem diameter in relation to plant water on terrestrial
plants in order to schedule the irrigation. The objective of this work
was to evaluate the ability of the strain gauges to capture changes in
the epiphytes plant stem. Experiments were carried out by using the
sympodial orchid, Dendrobium Sonia in a stressed condition. From
the findings, the sensor can detect changes in the plant stem and the
result can easily be used as a reference for further studies for the
development of a proper watering system.
Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
Abstract: This research was aimed at determining the impact of conservation techniques including bench terrace, stone terrace, mulching, grass strip and intercropping on soil erosion at tobacco-based farming system at Progo Hulu subwatershed, Central Java, Indonesia. Research was conducted from September 2007 to September 2009, located at Progo Hulu subwatershed, Central Java, Indonesia. Research site divided into 27 land units, and experimental fields were grouped based on the soil type and slope, ie: 30%, 45% and 70%, with the following treatments: 1) ST0= stone terrace (control); 2) ST1= stone terrace + Setaria spacelata grass strip on a 5 cm height dike at terrace lips + tobacco stem mulch with dose of 50% (7 ton/ ha); 3) ST2= stone terrace + Setaria spacelata grass strip on a 5 cm height dike at terrace lips + tobacco stem mulch with dose of 100% (14 ton/ ha); 4) ST3= stone terrace + tobacco and red bean intercropping + tobacco stem mulch with dose of 50% (7 ton/ ha). 5) BT0= bench terrace (control); 6) BT1= bench terrace + Setaria spacelata grass strip at terrace lips + tobacco stem mulch with dose of 50% (7 ton/ ha); 7) BT2= bench terrace + Setaria spacelata grass strip at terrace lips + tobacco stem mulch with dose of 100% (14 ton/ ha); 8) BT3= bench terrace + tobacco and red bean intercropping + tobacco stem mulch with dose of 50% (7 ton/ ha). The results showed that the actual erosion rates of research site were higher than that of tolerance erosion with mean value 89.08 ton/ha/year and 33.40 ton/ha/year, respectively. These resulted in 69% of total research site (5,119.15 ha) highly degraded. Conservation technique of ST2 was the most effective in suppressing soil erosion, by 42.87%, following with BT2 as much 30.63%. Others suppressed erosion only less than 21%.
Abstract: Aurein 1.2 is a 13-residue amphipathic peptide with antibacterial and anticancer activity. Aurein1.2 and its retro analog were synthesized to study the activity of the peptides in relation to their structure. The antibacterial test result showed the retro-analog is inactive. The secondary structural analysis by CD spectra indicated that both of the peptides at TFE/Water adopt alpha-helical conformation. MD simulation was performed on aurein 1.2 and retro-analog in water and TFE in order to analyse the factors that are involved in the activity difference between retro and the native peptide. The simulation results are discussed and validated in the light of experimental data from the CD experiment. Both of the peptides showed a relatively similar pattern for their hydrophobicity, hydrophilicity, solvent accessible surfaces, and solvent accessible hydrophobic surfaces. However, they showed different in directions of dipole moment of peptides. Also, Our results further indicate that the reversion of the amino acid sequence affects flexibility .The data also showed that factors causing structural rigidity may decrease the activity. Consequently, our finding suggests that in the case of sequence-reversed peptide strategy, one has to pay attention to the role of amino acid sequence order in making flexibility and role of dipole moment direction in peptide activity. KeywordsAntimicrobial peptides, retro, molecular dynamic, circular dichroism.
Abstract: Transcription factor p53 has a powerful tumor
suppressing function that is associated with many cancers. However,
p53 of the molecular weight was higher make the limitation across to
skin or cell membrane. Thymidine dinucleotide (pTT), an
oligonucleotide, can activate the p53 transcription factor. pTT is a
hydrophilic and negative charge oligonucleotide, which delivery in to
cell membrane need an appropriate carrier. The aim of this study was
to improve the bioavailability of the nucleotide fragment, thymidine
dinucleotide (pTT), using elasic liposome carriers to deliver the drug
into the skin. The study demonstrate that dioleoylphosphocholine
(DOPC) incorporated with sodium cholate at molar ratio 1:1 can
archived the particle size about 220 nm. This elastic liposome could
penetration through skin from stratum corneum to whole epidermis by
confocal laser scanning microscopy (CLSM). Moreover, we observed
the the slight increase in generation of p53 by western blot.
Abstract: The field research was carried out at the Látókép AGTC KIT research area of the University of Debrecen in Eastern-Hungary, on the area of the aeolain loess of the Hajdúság. We examined the effects of the sowing time on the phytopathogenic characteristics and yield production by applying various fertilizer treatments on two different sunflower genotypes (NK Ferti, PR64H42) in 2012 and 2013. We applied three different sowing times (early, optimal, late) and two different treatment levels of fungicides (control = no fungicides applied, double fungicide protection).
During our investigations, the studied cropyears were of different sowing time optimum in terms of yield amount (2012: early, 2013: average). By Pearson’s correlation analysis, we have found that delaying the sowing time pronouncedly decreased the extent of infection in both crop years (Diaporthe: r=0.663**, r=0.681**, Sclerotinia: r=0.465**, r=0.622**). The fungicide treatment not only decreased the extent of infection, but had yield increasing effect too (2012: r=0.498**, 2013: r=0.603**). In 2012, delaying of the sowing time increased (r=0.600**), but in 2013, it decreased (r= 0.356*) the yield amount.
Abstract: The Kumamoto area, Kyushu, Japan has 1,041km2 in
area and about 1milion in population. This area is a greatest area in Japan which depends on groundwater for all of drinking water. Quantity of this local groundwater use is about 200MCM during the
year. It is understood that the main recharging area of groundwater exist in the rice field zone which have high infiltrate height ahead of
100mm/ day of the irrigated water located in the middle area of the Shira-River Basin. However, by decrease of the paddy-rice planting
area by urbanization and an acreage reduction policy, the groundwater income and expenditure turned worse. Then Kumamoto city and four
companies expended financial support to increase recharging water to
underground by ponded water in the field from 2004.
In this paper, the author reported the situation of recovery of groundwater by recharge and estimates the efficiency of recharge by
statistical method.
Abstract: To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.
Abstract: This paper analysed the food security situation among
Nigerian rural farmers. Data collected on 202 rural farmers from
Benue State were analysed using descriptive and inferential statistics.
The study revealed that majority of the respondents (60.83%) had
medium dietary diversity. Furthermore, household daily calorie
requirement for the food secure households was 10,723 and the
household daily calorie consumption was 12,598, with a surplus
index of 0.04. The food security index was 1.16. The Household
daily per capita calorie consumption was 3,221.2. For the food
insecure households, the household daily calorie requirement was
20,213 and the household daily calorie consumption was 17,393. The
shortfall index was 0.14. The food security index was 0.88. The
Household daily per capita calorie consumption was 2,432.8. The
most commonly used coping strategies during food stress included
intercropping (99.2%), reliance on less preferred food (98.1%),
limiting portion size at meal times (85.8%) and crop diversification
(70.8%).
Abstract: Atlantic herring (Clupea harengus) is an important
commercial fish and shows to be more and more demanded for
human consumption. Therefore, it is very important to find good
methods for monitoring the freshness of the fish in order to keep it in
the best quality for human consumption. In this study, the fish was
stored in ice up to 2 weeks. Quality changes during storage were
assessed by the Quality Index Method (QIM), quantitative
descriptive analysis (QDA) and Torry scheme, by texture
measurements: puncture tests and Texture Profile Analysis (TPA)
tests on texture analyzer TA.XT2i, and by electronic nose (e-nose)
measurements using FreshSense instrument. Storage time of herring
in ice could be estimated by QIM with ± 2 days using 5 herring per
lot. No correlation between instrumental texture parameters and
storage time or between sensory and instrumental texture variables
was found. E-nose measurements could be use to detect the onset of
spoilage.