Abstract: Raw wood vinegar was purified by both standing and
filtering methods. Toxicity tests were conducted under laboratory
conditions by the topical application method (contact poison) and
feeding method (stomach poison). Larvicidal activities of wood
vinegar at four different concentrations (10, 15, 20, 25 and 30 %)
were studied against second instar larvae of housefly (Musca
domestica L.). Four replicates were maintained for all treatments and
controls. Larval mortality was recorded up to 96 hours and compared
with the larval survivability by two methods of larvicidal bioassay.
Percent pupation and percent adult emergence were observed in
treated M. domestica. The study revealed that the feeding method
gave higher efficiency compared with the topical application method.
Larval mortality increased with increasing concentration of wood
vinegar and the duration of exposure. No mortality was found in
treated M. domestica larvae at minimum 10% concentration of wood
vinegar through the experiments. The treated larvae were maintained
up to pupa and adult emergence. At 30% maximum concentration
larval duration was extended to 11 days in M. domestica for topical
application method and 9 days for feeding method. Similarly the
pupal durations were also increased with increased concentrations
(16 and 24 days for topical application method and feeding method
respectively at 30% concentration) of the treatments.
Abstract: Thrombosis can be life threatening, necessitating therefore its instant treatment. Hydergine, a nootropic agent is used as a cognition enhancer in stroke patients but relatively little is known about its anti-thrombolytic effect. To investigate this aspect, in vivo and ex vivo experiments were designed and conducted. Three groups of rats were injected 1.5mg, 3.0mg and 4.5mg hydergine intraperitonealy with and without prior exposure to fresh plasma. Positive and negative controls were run in parallel. Animals were sacrificed after 1.5hrs and BT, CT, PT, INR, APTT, plasma calcium levels were estimated. For ex vivo analyses, each 1ml blood aspirated was exposed to 0.1mg, 0.2mg, 0.3mg dose of hydergine with parallel controls. Parameters analyzed were as above. Statistical analysis was through one-way ANOVA. Dunken-s and Tukey-s tests provided intra-group variance. BT, CT, PT, INR and APTT increased while calcium levels dropped significantly (P
Abstract: 115 samples of Labeo calbasu ranged 8.0-17.9cm
length with mean11.90±1.96 and 4.9-68.5g weight with mean
22.25±12.54 from the River Chenab, Southern Punjab, Pakistan were
analyzed to investigate length-weight relationships (LWR) of fish in
relation to condition factor (K). Standard length (SL), fork length
(FL), head length (HL) head width (HW), body girth (BG), dorsal fin
length (DFL), dorsal fin base (DFB), pectoral fin length (PcFL),
pelvic fin length (PvFL) and anal fin length (AFL) are found to be
highly correlated with increasing total length and wet body weight (r
> 0.500). Wet body weight has positive (r=0.540) and total length
has no correlation (r=0.344) with calculated Condition factor (K).
The slope “b" in the relationship is 3.27 and intercepts -2.2258.
Abstract: The effects of global warming on India vary from the
submergence of low-lying islands and coastal lands to the melting of
glaciers in the Indian Himalayas, threatening the volumetric flow rate
of many of the most important rivers of India and South Asia. In
India, such effects are projected to impact millions of lives. As a
result of ongoing climate change, the climate of India has become
increasingly volatile over the past several decades; this trend is
expected to continue.
Climate change is one of the most important global environmental
challenges, with implications for food production, water supply,
health, energy, etc. Addressing climate change requires a good
scientific understanding as well as coordinated action at national and
global level. The climate change issue is part of the larger challenge
of sustainable development. As a result, climate policies can be more
effective when consistently embedded within broader strategies
designed to make national and regional development paths more
sustainable. The impact of climate variability and change, climate
policy responses, and associated socio-economic development will
affect the ability of countries to achieve sustainable development
goals.
A very well calibrated Soil and Water Assessment Tool (R2 =
0.9968, NSE = 0.91) was exercised over the Khatra sub basin of the
Kangsabati River watershed in Bankura district of West Bengal,
India, in order to evaluate projected parameters for agricultural
activities. Evapotranspiration, Transmission Losses, Potential
Evapotranspiration and Lateral Flow to reach are evaluated from the
years 2041-2050 in order to generate a picture for sustainable
development of the river basin and its inhabitants.
India has a significant stake in scientific advancement as well as
an international understanding to promote mitigation and adaptation.
This requires improved scientific understanding, capacity building,
networking and broad consultation processes. This paper is a
commitment towards the planning, management and development of
the water resources of the Kangsabati River by presenting detailed
future scenarios of the Kangsabati river basin, Khatra sub basin, over
the mentioned time period.
India-s economy and societal infrastructures are finely tuned to the
remarkable stability of the Indian monsoon, with the consequence
that vulnerability to small changes in monsoon rainfall is very high.
In 2002 the monsoon rains failed during July, causing profound loss
of agricultural production with a drop of over 3% in India-s GDP.
Neither the prolonged break in the monsoon nor the seasonal rainfall
deficit was predicted. While the general features of monsoon
variability and change are fairly well-documented, the causal
mechanisms and the role of regional ecosystems in modulating the
changes are still not clear. Current climate models are very poor at
modelling the Asian monsoon: this is a challenging and critical
region where the ocean, atmosphere, land surface and mountains all
interact. The impact of climate change on regional ecosystems is
likewise unknown. The potential for the monsoon to become more
volatile has major implications for India itself and for economies
worldwide. Knowledge of future variability of the monsoon system,
particularly in the context of global climate change, is of great
concern for regional water and food security.
The major findings of this paper were that of all the chosen
projected parameters, transmission losses, soil water content,
potential evapotranspiration, evapotranspiration and lateral flow to
reach, display an increasing trend over the time period of years 2041-
2050.
Abstract: The aim of the present investigation was to compare
sex differences in thyroid gland structure of rabbits. Five adult male
and five adult female (3.1-3.5 kg body weight) New Zealand white
rabbits were used in the experiment. Results showed that at light
microscopic level, there was no sex difference in microscopic
appearance of the thyroid glands. At electron microscopic level,
however, the mitochondria and the microvilli of the follicular cells
are more numerous and the Golgi complex is also more extensive in
male rabbits in comparison to females. Results obtained from
micrometric measurements showed that the volume density of the
follicles is higher in males than in females, but the differences are not
statistically significant .The volume density of epithelium and the
height of follicular cells are significantly greater in males than in
females and reverse is true about the volume density of interstitium
(p
Abstract: Nigella sativa L. is an aromatic plant belonging to the
family Ranunculaceae. It has been used traditionally, especially in the
middle East and India, for the treatment of asthma, cough, bronchitis,
headache, rheumatism, fever, influenza and eczema. Several
biological activities have been reported in Nigella sativa seeds,
including antioxidant. In this context we tried to estimate the
antioxidant activity of various extracts prepared from Nigella sativa
seeds, methanolic extract (ME), chloroformic extract (CE), hexanic
extract (HE : fixed oil), ethyl acetate extract (EAE) water extract
(WE). The Folin-Ciocalteu assay showed that CE and EAE contained
high level of phenolic compounds 81.31 and 72.43μg GAE/mg of
extract respectively. Similarly, the CE and EAE exhibited the highest
DPPH radical scavenging activity, with IC50 values of 106.56μg/ml
and 121.62μg/ml respectively. In addition, CE and HE showed the
most scavenging activity against superoxide radical generated in the
PMS-NADH-NBT system with respective IC50 values of 361.86
μg/ml and 371.80 μg/ml, which is comparable to the activity of the
standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating
capacity assay showed that WE, EAE and ME are the most active
with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition
of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene
bleaching assay, this showed a highest relative antioxidant
activity with CE and EAE (69.82% of inhibition). The antioxidant
activities of the methanolic extract and the fixed oil are confirmed by
an in vivo assay in mice, the daily oral administration of methanolic
extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day)
during 21 days, resulted in a significant enhancement of the blood
total antioxidant capacity (measured by KRL test) and the plasmatic
antioxidant capacity towards DPPH radical.
Abstract: Nuts are part of a healthy diet such as Mediterranean diet. Benefits of nuts in reducing the risk of heart disease has been reasonably attributed to their composition of vitamins, minerals, unsaturated fatty acids, fiber and phytochemicals such as polyphenols, tocopherols, squalene and phytosterols. More than 75% of total fatty acids of nuts are unsaturated. α- tocopherol is the main tocopherol isomer present in most of the nuts. While walnuts, Brazil nut, cashew nut, peanut, pecan and pistachio nuts are rich in γ- tocopherol. β- sitosterol is dominant sterol in nuts. Pistachio and pine nut have the highest total phytosterol and Brazil nut and English walnut the lowest. Walnuts also contain large amount of phenolic compounds compared with other nuts. Nuts are rich in compounds with antioxidant properties and their consumption can offer preventing from incidence of many diseases including cardiovascular.
Abstract: MMR vaccine failure had been reported globally and
here we report that it occurs now in India. Samples were collected from clinically suspected mumps cases were subjected for anti
mumps antibodies, virus isolation, RT-PCR, sequencing and
phylogenetic tree analysis. 56 samples collected from men and women belonging to various age groups. 30 had been vaccinated and
the status of 26 patients was unknown. 28 out of 30 samples were
found to be symptomatic and positive for Mumps IgM, indicating
active mumps infection in 93.4% of the vaccinated population. A
phylogenetic tree comparison of the clinical isolate is shown to be genotype C which is distinct from vaccine strain. Our study clearly sending warning signs that MMR vaccine is a failure and it needs to be revamped for the human use by increasing its efficacy and efficiency.
Abstract: One of the major problems in genomic field is to perform sequence comparison on DNA and protein sequences. Executing sequence comparison on the DNA and protein data is a computationally intensive task. Sequence comparison is the basic step for all algorithms in protein sequences similarity. Parallel computing is an attractive solution to provide the computational power needed to speedup the lengthy process of the sequence comparison. Our main research is to enhance the protein sequence algorithm using dynamic programming method. In our approach, we parallelize the dynamic programming algorithm using multithreaded program to perform the sequence comparison and also developed a distributed protein database among many PCs using Remote Method Interface (RMI). As a result, we showed how different sizes of protein sequences data and computation of scoring matrix of these protein sequence on different number of processors affected the processing time and speed, as oppose to sequential processing.
Abstract: The estrus female Etawah cross bred goats were
synchronized estrus by controlled internal drug release (CIDR)
implants for 10 days combined with PGF2α injection, and continued
by artificial insemination (AI) within the hours of 24 period. Vaginal
epithelium was taken to determine estrus cycle of the goats without
estrus synchronization. The estrus responds (the puffy of vulva and
vaginal pH) and percentage of pregnancy were investigated. The data
were analyzed descriptively and Independent Sample T-Test. The
results showed that the puffy of vulva and vaginal pH were
significantly different in synchronized estrus goats and control goats
(2.18 ± 0.33 cm vs. 1.20 ± 0.16 cm and 8.55 ± 0.63 vs. 8.22 ± 0.22).
Percentage of pregnancy was higher in synchronized estrus goats
(73.33%) than in control (53.3%). Estrus synchronization by using
CIDR implants and PGF2, continued by AI was effective to improve
reproduction performance of Etawah cross bred goats.
Abstract: Foodborne Salmonella infections have become a
major problem world wide. Salmonellosis transmitted from fish are
quite common. Established quality control measures exist for export
oriented fish, none exists for fish consumed locally. This study aimed
at characterization of Salmonella isolated from Nile tilapia . The
study was carried out in selected beaches along L. Victoria in
Western Kenya between March and June 2007. One hundred and
twenty fish specimens were collected. Salmonella isolates were
confirmed using serotyping, biochemical testing in addition to malic
acid dehydrogenase (mdh) and fliC gene sequencing. Twenty
Salmonella isolates were confirmed by mdh gene sequencing. Nine
(9) were S. enterica serotype typhimurium, four (4) were S. enterica
Serotype, enteritidis and seven (7) were S. enterica serotype typhi.
Nile tilapia have a role in transmission of Salmonellosis in the study
area, poor sanitation was a major cause of pollution at the beach
inshore waters.
Abstract: Three batches of yogurts were made with soy protein
isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of
skim milk powder (SMP). The fourth batch (control; S0) was
prepared from SPI without SMP supplementation. Lactobacillus
delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and
Streptococcus thermophilus ST 1342 (ST 1342) were used as the
starter culture. Biotransformation of the inactive forms, isoflavone
glycosides (IG) to biologically active forms, isoflavone aglycones
(IA), was determined during 28 d storage. The viability of both
microorganisms was significantly higher (P < 0.05) in S2, S4, and S6
than that in S0. The ratio of lactic acid/acetic acid in S0 was in the
range of 15.53 – 22.31 compared to 7.24 – 12.81 in S2, S4 and S6.
The biotransformation of IG to IA in S2, S4 and S6 was also
enhanced by 9.9 -13.3% compared to S0.
Abstract: Categorical data based on description of the
agricultural landscape imposed some mathematical and analytical
limitations. This problem however can be overcome by data
transformation through coding scheme and the use of non-parametric
multivariate approach. The present study describes data
transformation from qualitative to numerical descriptors. In a
collection of 103 random soil samples over a 60 hectare field,
categorical data were obtained from the following variables: levels of
nitrogen, phosphorus, potassium, pH, hue, chroma, value and data on
topography, vegetation type, and the presence of rocks. Categorical
data were coded, and Spearman-s rho correlation was then calculated
using PAST software ver. 1.78 in which Principal Component
Analysis was based. Results revealed successful data transformation,
generating 1030 quantitative descriptors. Visualization based on the
new set of descriptors showed clear differences among sites, and
amount of variation was successfully measured. Possible applications
of data transformation are discussed.
Abstract: Larval rearing and seed production of most of tetra fishes (Family: Characidae) is critical due to their small size larvae and limited numbers of spawning attempts. During the present study the effect of different live foods on growth and survival of neon tetra, Paracheirodon innesi larvae (length 3.1 ± 0.012mm, weight 0.048 ± 0.00015mg) and early fry (length = 6.44 ± 0.025mm, weight = 0.64 ± 0.003mg and 13 days old) was determined in two experiments. Experiment I was conducted for rearing the larvae by using mixed green water and Infusoria whereas, in Experiment II, early fry were fed with mixed zooplankton, decapsulated Artemia cyst and Artemia nauplii. The larvae fed on mixed green water showed significant (p
Abstract: Thailand is one of the world-s leaders of rice
producers and exporters. Farmers have to increase the rice cultivation
frequency for serving the national increasing of export-s demand. It
leads to an elimination of rice residues by open burning which is the
quickest and costless management method. The open burning of rice
residue is one of the major causes of air pollutants and greenhouse
gas (GHG) emission. Under ASEAN agreement on trans-boundary
haze, Thailand set the master plan to mitigate air pollutant emission
from open burning of agricultural residues. In this master plan,
residues incorporation is promoted as alternative management
method to open burning. However, the assessment of both options in
term of GHG emission in order to investigate their contribution to
long-term global warming is still scarce or inexistent. In this study, a
method on rice residues assessment was first developed in order to
estimate and compare GHG emissions from rice cultivation under
rice residues open burning and the case with incorporation of the
same amount of rice residues, using 2006 IPCC guidelines for
emission estimation and Life Cycle Analysis technique. The
emission from rice cultivation in different preparing area practice
was also discussed.
Abstract: In this paper, perceptions of actors on changes in
crop productivity, quantity and quality of water, and determinants of
their perception are analyzed using descriptive statistics and ordered
logit model. Data collected from 297 Ethiopian farmers and 103
agricultural professionals from December 2009 to January 2010 are
employed. Results show that the majority of the farmers and
professionals recognized decline in water resources, reasoning
climate changes and soil erosion as some of the causes. However,
there is a variation in views on changes in productivity. The
household asset, education level, age and geographical positions are
found to affect farmers- perception on changes in crop productivity.
But, the study underlines that there is no evidence that farmers-
economic status, age, or education level affects recognition of
degradation of water resources. Thus, more focus shall be given on
providing them different coping mechanisms and alternative
resource conserving technologies than educating about the
problems.
Abstract: This paper is to present context-aware sensor grid
framework for agriculture and its design challenges. Use of sensor
networks in the domain of agriculture is not new. However, due to
the unavailability of any common framework, solutions that are
developed in this domain are location, environment and problem
dependent. Keeping the need of common framework for agriculture,
Context-Aware Sensor Grid Framework is proposed. It will be
helpful in developing solutions for majority of the problems related
to irrigation, pesticides spray, use of fertilizers, regular monitoring of
plot and yield etc. due to the capability of adjusting according to
location and environment. The proposed framework is composed of
three layer architecture including context-aware application layer,
grid middleware layer and sensor network layer.
Abstract: The role of the pollen grain, with to the reproductive
process of higher plants, is to deliver the spermatic cells to the
embryo sac for egg fertilization. The aim of this project was study
the effect of electromagnetic fields on structure and pollen grains
development in Chenopodium album. Anthers of Chenopodium
album L. were collected at different stages of development from
control (without electromagnetic field) and plants grown at 10m from
the field sources. Structure and development of pollen grains were
studied and compared. The studying pollen structure by Light and
Scanning electron microscopy showed that electromagnetic fields
reduction of pollen grains number and male sterility, thus , in some
anthers, pollen grains were attached together and deformed compared
to control ones. The data presented suggest that prolonged exposures
of plants to magnetic field may cause different biological effects at
the cellular tissue and organ levels.
Abstract: The effect of varying holding temperature on hatching success, occurrence of deformities and mortality rates were investigated for goldlined seabream eggs. Wild broodstock (600 g) were stocked at a 2:1 male-female ratio in a 2 m3 fiberglass tank supplied with filtered seawater (37 g L-1 salinity, temp. range 24±0.5 oC [day] and 22±1 oC [night], DO2 in excess of 5.0mg L-1). Females were injected with 200 IU kg-1 HCG between 08.00 and 10.00 h and returned to tanks to spawn following which eggs were collected by hand using a 100μm net. Fertilized eggs at the gastrulation stage (120 L-1) were randomly placed into one of 12 experimental 6 L aerated (DO2 5 mg L-1) plastic containers with water temperatures maintained at 24±0.5 oC (ambient), 26±0.5 oC, 28± 0.5 oC and 30±0.5 oC using thermostats. Each treatment was undertaken in triplicate using a 12:12 photophase:scotophase photoperiod. No differences were recorded between eggs reared at 24 and 26 oC with respect to viability, deformity, mortality or unhatched egg rates. Increasing temperature reduced the number of viable eggs with those at 30 oC returning poorest performance (P < 0.05). Mortality levels were lowest for eggs incubated at 24 and 26 oC. The greatest level of deformities recorded was that for eggs reared at 28 oC.
Abstract: Silicon is a beneficial element for plant growth. It
helps plants to overcome multiple stresses, alleviates metal toxicity
and improves nutrient imbalance. Field experiment was conducted as
split-split plot arranged in a randomized complete block design with
four replications. Irrigation system include continues flooding and
deficit as main plots and nitrogen rates N0, N46, N92, and N138 kg/ha
as sub plots and silicon rates Si0 & Si500 kg/ha as sub-subplots.
Results indicate that grain yield had not significant difference
between irrigation systems. Flooding irrigation had higher biological
yield than deficit irrigation whereas, no significant difference in grain
and straw yield. Nitrogen application increased grain, biological and
straw yield. Silicon application increased grain, biological and straw
yield but, decreased harvest index. Flooding irrigation had higher
number of total tillers / hill than deficit irrigation, but deficit
irrigation had higher number of fertile tillers / hill than flooding
irrigation. Silicon increased number of filled spikelet and decreased
blank spikelet. With high nitrogen application decreased 1000-grain
weight. It can be concluded that if the nitrogen application was high
and water supplied was available we could have silicon application
until increase grain yield.