Abstract: Morphological interaction of porcine cumulus-oocyte
complexes (pCOCs) was investigated on in vitro condition using
electron microscope (SEM and TEM). The totals of 1,923 oocytes
were round in shape, surrounded by Zona pellucida with layer of
cumulus cells ranging between 59.29-202.14 μm in size. They were
classified into intact-, multi-, partial cumulus cell layer oocyte, and
completely denuded oocyte, at the percentage composition of 22.80%
32.70%, 18.60%, and 25.90 % respectively. The pCOCs classified as
intact- and multi cumulus cell layer oocytes were further culturing at
37°C with 5% CO2, 95% air atmosphere and high humidity for 44 h
in M199 with Earle’s salts supplemented with 10% HTFCS, 2.2
mg/mL NaHCO3, 1 M Hepes, 0.25 mM pyruvate, 15 μg/mL porcine
follicle-stimulating hormone, 1 μg/mL LH, 1μg/mL estradiol with
ethanol, and 50 μg/mL gentamycin sulfate. On electron microscope
study, cumulus cells were found to stick their processes to secrete
substance from the sac-shape end into Zona pellucida of the oocyte
and also communicated with the neighboring cells through their
microvilli on the beginning of incubation period. It is believed that
the cumulus cells communicate with the oocyte by inserting the
microvilli through this gap and embedded in the oocyte cytoplasm
before secreting substance, through the sac-shape end of the
microvilli, to inhibit primary oocyte development at the prophase I.
Morphological changes of the complexes were observed after
culturing for 24-44 h. One hundred percentages of the cumulus layers
were expanded and cumulus cells were peeling off from the oocyte
surface. In addition, the round-shape cumulus cells transformed
themselves into either an elongate shape or a columnar shape, and no
communication between cumulus neighboring cells. After 44 h of
incubation time, diameter of oocytes surrounded by cumulus cells
was larger than 0 h incubation. The effect of hormones in culture
medium is exerted by their receptors present in porcine oocyte. It is
likely that all morphological changes of the complexes after hormone
treatment were to allow maturation of the oocyte. This study
demonstrated that the association of hormones in M199 could
promote porcine follicle activation in 44 h in vitro condition. This
culture system should be useful for studying the regulation of early
follicular growth and development, especially because these follicles represent a large source of oocytes that could be used in vitro for cell
technology.
Abstract: Machining of hard materials is a recent technology for
direct production of work-pieces. The primary challenge in
machining these materials is selection of cutting tool inserts which
facilitates an extended tool life and high-precision machining of the
component. These materials are widely for making precision parts for
the aerospace industry. Nickel-based alloys are typically used in
extreme environment applications where a combination of strength,
corrosion resistance and oxidation resistance material characteristics
are required. The present paper reports the theoretical and
experimental investigations carried out to understand the influence of
machining parameters on the response parameters. Considering the
basic machining parameters (speed, feed and depth of cut) a study has
been conducted to observe their influence on material removal rate,
surface roughness, cutting forces and corresponding tool wear.
Experiments are designed and conducted with the help of Central
Composite Rotatable Design technique. The results reveals that for a
given range of process parameters, material removal rate is favorable
for higher depths of cut and low feed rate for cutting forces. Low feed
rates and high values of rotational speeds are suitable for better finish
and higher tool life.
Abstract: Beta-glucosidase, chitinase, leucine-aminopeptidase, acid phosphomonoesterase and acetate-esterase enzyme activities in
the soils under the impact of metallurgical industrial activity in Lori
marz (district) were investigated. The results of the study showed that
the activities of the investigated enzymes in the soils decreased with increasing distance from the Shamlugh copper mine, the Chochkan
tailings storage facility and the ore transportation road. Statistical
analysis revealed that the activities of the enzymes were positively
correlated (significant) to each other according to the observation
sites which indicated that enzyme activities were affected by the
same anthropogenic factor. The investigations showed that the soils
were polluted with heavy metals (Cu, Pb, As, Co, Ni, Zn) due to
copper mining activity in this territory. The results of Pearson
correlation analysis revealed a significant negative correlation
between heavy metal pollution degree (Nemerow integrated pollution
index) and soil enzyme activity. All of this indicated that copper
mining activity in this territory causing the heavy metal pollution of
the soils resulted in the inhabitation of the activities of the enzymes
which are considered as biological catalysts to decompose organic
materials and facilitate the cycling of nutrients.
Abstract: The key role in phenomenological modelling of cyclic
plasticity is good understanding of stress-strain behaviour of given
material. There are many models describing behaviour of materials
using numerous parameters and constants. Combination of individual
parameters in those material models significantly determines whether
observed and predicted results are in compliance. Parameter
identification techniques such as random gradient, genetic algorithm
and sensitivity analysis are used for identification of parameters using
numerical modelling and simulation. In this paper genetic algorithm
and sensitivity analysis are used to study effect of 4 parameters of
modified AbdelKarim-Ohno cyclic plasticity model. Results
predicted by Finite Element (FE) simulation are compared with
experimental data from biaxial ratcheting test with semi-elliptical
loading path.
Abstract: Properties of Portland cement mixtures with various
fractions of metakaolin were studied. 10% of Portland cement CEM I
42.5 R was replaced by different fractions of high reactivity
metakaolin with defined chemical and mineralogical properties.
Various fractions of metakaolin were prepared by jet mill classifying
system. There is a clear trend between fineness of metakaolin and
hydration heat development. Due to metakaolin presence in mixtures
the compressive strength development of mortars is rather slower for
coarser fractions but 28-day flexural strengths are improved for all
fractions of metakaoline used in mixtures compared to reference
sample of pure Portland cement. Yield point, plastic viscosity and
adhesion of fresh pastes are considerably influenced by fineness of
metakaolin used in cement pastes.
Abstract: The value co-creation has gained much attention in
sales research, but less is known about how salespeople and
customers interact in the authentic business to business (B2B) sales
meetings. The study presented in this paper empirically contributes to
existing research by presenting authentic B2B sales meetings that
were video recorded and analyzed using observation and qualitative
content analysis methods. This paper aims to study key elements of
successful sales interactions between salespeople and customers/
buyers. This study points out that salespeople are selling value rather
than the products or services themselves, which are only enablers in
realizing business benefits. Therefore, our findings suggest that
promoting and easing open discourse is an essential part of a
successful sales encounter. A better understanding of how
salespeople and customers successfully interact would help
salespeople to develop their interpersonal sales skills.
Abstract: Two finite element (FEM) models are presented in
this paper to address the random nature of the response of glued
timber structures made of wood segments with variable elastic
moduli evaluated from 3600 indentation measurements. This total
database served to create the same number of ensembles as was the
number of segments in the tested beam. Statistics of these ensembles
were then assigned to given segments of beams and the Latin
Hypercube Sampling (LHS) method was called to perform 100
simulations resulting into the ensemble of 100 deflections subjected
to statistical evaluation. Here, a detailed geometrical arrangement of
individual segments in the laminated beam was considered in the
construction of two-dimensional FEM model subjected to in fourpoint
bending to comply with the laboratory tests. Since laboratory
measurements of local elastic moduli may in general suffer from a
significant experimental error, it appears advantageous to exploit the
full scale measurements of timber beams, i.e. deflections, to improve
their prior distributions with the help of the Bayesian statistical
method. This, however, requires an efficient computational model
when simulating the laboratory tests numerically. To this end, a
simplified model based on Mindlin’s beam theory was established.
The improved posterior distributions show that the most significant
change of the Young’s modulus distribution takes place in laminae in
the most strained zones, i.e. in the top and bottom layers within the
beam center region. Posterior distributions of moduli of elasticity
were subsequently utilized in the 2D FEM model and compared with
the original simulations.
Abstract: The thermal control in many systems is widely
accomplished applying mixed convection process due to its low cost,
reliability and easy maintenance. Typical applications include the
aircraft electronic equipment, rotating-disc heat exchangers, turbo
machinery, and nuclear reactors, etc. Natural convection in an inclined
square enclosure heated via wall heater has been studied numerically.
Finite volume method is used for solving momentum and energy
equations in the form of stream function–vorticity. The right and left
walls are kept at a constant temperature, while the other parts are
adiabatic. The range of the inclination angle covers a whole revolution.
The method is validated for a vertical cavity. A general power law
dependence of the Nusselt number with respect to the Rayleigh
number with the coefficient and exponent as functions of the
inclination angle is presented. For a fixed Rayleigh number, the
inclination angle increases or decreases is found.
Abstract: To determine the potential of a low cost Irish
engineered timber product to replace high cost solid timber for use in
bending active structures such as gridshells a single Irish engineered
timber product in the form of orientated strand board (OSB) was
selected. A comparative study of OSB and solid timber was carried
out to determine the optimum properties that make a material suitable
for use in gridshells. Three parameters were identified to be relevant
in the selection of a material for gridshells. These three parameters
are the strength to stiffness ratio, the flexural stiffness of
commercially available sections, and the variability of material and
section properties. It is shown that when comparing OSB against
solid timber, OSB is a more suitable material for use in gridshells that
are at the smaller end of the scale and that have tight radii of
curvature. Typically, for solid timber materials, stiffness is used as an
indicator for strength and engineered timber is no different. Thus, low
flexural stiffness would mean low flexural strength. However, when
it comes to bending active gridshells, OSB offers a significant
advantage. By the addition of multiple layers, an increased section
size is created, thus endowing the structure with higher stiffness and
higher strength from initial low stiffness and low strength materials
while still maintaining tight radii of curvature. This allows OSB to
compete with solid timber on large scale gridshells. Additionally, a
preliminary sustainability study using a set of sustainability indicators
was carried out to determine the relative sustainability of building a
large-scale gridshell in Ireland with a primary focus on economic
viability but a mention is also given to social and environmental
aspects. For this, the Savill garden gridshell in the UK was used as
the functional unit with the sustainability of the structural roof
skeleton constructed from UK larch solid timber being compared
with the same structure using Irish OSB. Albeit that the advantages of
using commercially available OSB in a bending active gridshell are
marginal and limited to specific gridshell applications, further study
into an optimised engineered timber product is merited.
Abstract: Most people today are aware that global climate
change is not just a scientific theory but also a fact with worldwide
consequences. Global climate change is due to rapid urbanization,
industrialization, high population growth and current vulnerability of
the climatic condition. Water is becoming scarce as a result of global
climate change. To mitigate the problem arising due to global climate
change and its drought effect, harvesting rainwater from green roofs,
an environmentally-friendly and versatile technology, is becoming
one of the best assessment criteria and gaining attention in Malaysia.
This paper addresses the sustainability of green roofs and examines
the quality of water harvested from green roofs in comparison to
rainwater. The factors that affect the quality of such water, taking
into account, for example, roofing materials, climatic conditions, the
frequency of rainfall frequency and the first flush. A green roof was
installed on the Humid Tropic Centre (HTC) is a place of the study
on monitoring program for urban Stormwater Management Manual
for Malaysia (MSMA), Eco-Hydrological Project in Kuala Lumpur,
and the rainwater was harvested and evaluated on the basis of four
parameters i.e., conductivity, dissolved oxygen (DO), pH and
temperature. These parameters were found to fall between Class I and
Class III of the Interim National Water Quality Standards (INWQS)
and the Water Quality Index (WQI). Some preliminary treatment
such as disinfection and filtration could likely to improve the value of
these parameters to class I. This review paper clearly indicates that
there is a need for more research to address other microbiological and
chemical quality parameters to ensure that the harvested water is
suitable for use potable water for domestic purposes. The change in
all physical, chemical and microbiological parameters with respect to
storage time will be a major focus of future studies in this field.
Abstract: Fungal mutant strains have produced cellulase and
xylanase enzymes, and have induced high hydrolysis with enhanced
of rice straw. The mutants were obtained by exposing Penicillium
strain to UV-light treatments. Screening and selection after treatment
with UV-light were carried out using cellulolytic and xylanolytic
clear zones method to select the hypercellulolytic and
hyperxylanolytic mutants. These mutants were evaluated for their
cellulase and xylanase enzyme production as well as their abilities for
biodegradation of rice straw. The mutant 12 UV/1 produced 306.21%
and 209.91% cellulase and xylanase, respectively, as compared with
the original wild type strain. This mutant showed high capacity of
rice straw degradation. The effectiveness of tested mutant strain and
that of wild strain was compared in relation to enhancing the
composting process of rice straw and animal manures mixture. The
results obtained showed that the compost product of inoculated
mixture with mutant strain (12 UV/1) was the best compared to the
wild strain and un-inoculated mixture. Analysis of the composted
materials showed that the characteristics of the produced compost
were close to those of the high quality standard compost. The results
obtained in the present work suggest that the combination between
rice straw and animal manure could be used for enhancing the
composting process of rice straw and particularly when applied with
fungal decomposer accelerating the composting process.
Abstract: This paper presents the results and findings from a
parametric study on the water surface elevation at upstream of bridge
constriction for subcritical flow. In this study, the influence of
Manning's Roughness Coefficient of main channel (nmc) and
floodplain (nfp), and bridge opening (b) flow rate (Q), contraction
(kcon) and expansion coefficients (kexp) were investigated on
backwater level. The DECK bridge models with different span widths
and without any pier were investigated within the two stage channel
having various roughness conditions. One of the most commonly
used commercial one-dimensional HEC-RAS model was used in this
parametric study. This study showed that the effects of main channel
roughness (nmc) and flow rate (Q) on the backwater level are much
higher than those of the floodplain roughness (nfp). Bridge opening
(b) with contraction (kcon) and expansion coefficients (kexp) have very
little effect on the backwater level within this range of parameters.
Abstract: The rhizome of Java grass, Cyperus rotundus was
extracted different organic polar and non-polar solvents and
performed the in vitro antiviral and immunostimulant activities
against White Spot Syndrome Virus (WSSV) and Vibrio harveyi
respectively. Based on the initial screening the ethyl acetate extract of
C. rotundus was strong activities and further it was purified through
silica column chromatography and the fractions were screened again
for antiviral and immunostimulant activity. Among the different
fractions screened against the WSSV and V. harveyi, the fractions, FIII
to FV had strong activities. In order to study the in vivo influence
of C. rotundus, the fractions (F-III to FV) were pooled and delivered
to the F. indicus through artificial feed for 30 days. After the feeding
trail the experimental and control diet fed F. indicus were challenged
with virulent WSSV and studied the survival, molecular diagnosis,
biochemical, haematological, and immunological parameters.
Surprisingly, the pooled fractions (F-IV to FVI) incorporated diets
helped to significantly (P
Abstract: The English competence of Thai people was examined
in the context of knowledge of English in everyday life for Small and
Medium Entrepreneurs (SMEs), and also integrated with Second
language acquisition (SLA) students’ classroom. Second language
acquisition was applied to the results of the questionnaires and
interview forms. Levels of the need on English used for SME
entrepreneurs in Thailand, satisfaction on joining the street classroom
project were shown to be significantly high for some certain language
functions and satisfaction. Finding suggests that the language
functions on etiquette for professional use is essential and useful
because lesson learned can be used in the real situation for their
career. Implications for the climate of the street classroom are
discussed.
Abstract: The concept of technology as well as itself has
evolved continuously over time, such that, nowadays, this concept is
still marked by myths and realities. Even the concept of science is
frequently misunderstood as technology. In this way, this paper
presents different forms of interpretation of the concept of technology
in the course of history, as well as the social and cultural aspects
associated with it, through an analysis made by means of insights
from sociological studies of science and technology and its multiple
relations with society. Through the analysis of contents, the paper
presents a classification of how technology is interpreted in the social
sphere and search channel efforts to show how a broader
understanding can contribute to better interpretations of how
scientific and technological development influences the environment
in which we operate. The text also presents a particular point of view
for the interpretation of the concept from the analysis throughout the
whole work.
Abstract: The management of Helicobacter pylori (H. pylori)
eradication is still a matter of discussion, full effectiveness is rarely
achieved, and it has many adverse effects. The use of probiotics may
be associated with better eradication rates and possibly prevention of
adverse events due to antibiotic therapy. The present clinical study
was undertaken to evaluate the efficacy of a specially designed
fermented milk product, containing Bifidobacterium lactis B420, on
the eradication of H. pylori infection in a prospective, randomized,
double-blind, controlled study in humans. Four test fermented milks
(FM) were specially designed in which counts of viable cells in all
products were 10^10 Log CFU. 100 mL-1 for Bifidobacterium lactis -
Bifidobacterium species 420. 190 subjects infected with H. pylori,
with previous diagnosis of functional dyspepsia according to Rome
III criteria entered the study. Bifidobacterium lactis B420,
administered twice a day for 90 days was not able to eradicate H.
pylori in Brazilian patients with functional dyspepsia.
Abstract: Vehicular Adhoc Network (VANET) is a new
technology which aims to ensure intelligent inter-vehicle
communications, seamless internet connectivity leading to improved
road safety, essential alerts, and access to comfort and entertainment.
VANET operations are hindered by mobile node’s (vehicles)
uncertain mobility. Routing algorithms use metrics to evaluate which
path is best for packets to travel. Metrics like path length (hop count),
delay, reliability, bandwidth, and load determine optimal route. The
proposed scheme exploits link quality, traffic density, and
intersections as routing metrics to determine next hop. This study
enhances Geographical Routing Protocol (GRP) using fuzzy
controllers while rules are optimized with Bee Swarm Optimization
(BSO). Simulations results are compared to conventional GRP.
Abstract: In this paper, two options of anodic alumina barrier
layer thinning have been demonstrated. The approaches varied with
the duration of the voltage step. It was found that too long step of the
barrier layer thinning process leads to chemical etching of the
nanopores on their top. At the bottoms pores are not fully opened
what is disadvantageous for further applications in nanofabrication.
On the other hand, while the duration of the voltage step is controlled
by the current density (value of the current density cannot exceed
75% of the value recorded during previous voltage step) the pores are
fully opened. However, pores at the bottom obtained with this
procedure have smaller diameter, nevertheless this procedure
provides electric contact between the bare aluminum (substrate) and
electrolyte, what is suitable for template assisted electrodeposition,
one of the most cost-efficient synthesis method in nanotechnology.
Abstract: This paper presents an adaptive thermal comfort
model study in the tropical country of Malaysia. A number of
researchers have been interested in applying the adaptive thermal
comfort model to different climates throughout the world, but so far
no study has been performed in Malaysia. For the use as a thermal
comfort model, which better applies to hot and humid climates, the
adaptive thermal comfort model was developed as part of this
research by using the collected results from a large field study in six
lecture halls with 178 students. The relationship between the
operative temperature and behavioral adaptations was determined. In
the developed adaptive model, the acceptable indoor neutral
temperatures lay within the range of 23.9-26.0C, with outdoor
temperatures ranging between 27.0-34.6C. The most comfortable
temperature for students in lecture hall was 25.7C.
Abstract: Ocimum americanum L (Lamiaceae) is an annual herb
that is native to tropical Africa. The in vitro and in vivo antioxidant
activity of its aqueous extract was carefully investigated by assessing
the DPPH radical scavenging activity, ABTS radical scavenging
activity and hydrogen peroxide radical scavenging activity. The
reducing power, total phenol, total flavonoids and flavonols content
of the extract were also evaluated. The data obtained revealed that the
extract is rich in polyphenolic compounds and scavenged the radicals
in a concentration dependent manner. This was done in comparison
with the standard antioxidants such as BHT and Vitamin C. Also, the
induction of oxidative damage with paracetamol (2000 mg/kg)
resulted in the elevation of lipid peroxides and significant (P < 0.05)
decrease in activities of superoxide dismutase, glutathione
peroxidase, glutathione reductase and catalase in the liver and kidney
of rats. However, the pretreatment of rats with aqueous extract of O.
americanum leaves (200 and 400 mg/kg) and silymarin (100 mg/kg)
caused a significant (P < 0.05) reduction in the values of lipid
peroxides and restored the levels of antioxidant parameters in these
organs. These findings suggest that the leaves of O. americanum have
potent antioxidant properties which may be responsible for its
acclaimed folkloric uses.