Abstract: Pomegranate (Punica granatum L.) is an ancient fruit of great medical interest and rich source of antioxidants. Pesticides as dimethoate play a crucial role in the occurrence many diseases in plants, animal and human. Therefore the ability of Pomegranate (Punica granatum L.) to alleviate hepatotoxicity induced by organophosphate pesticide dimethoate was investigated. Albino male rats were divided randomly into 4 groups and kept at 7 animals per group in an environmentally controlled condition for 6 weeks. The first group was served as a control group (basal diet), the second group fed on basal diet supplemented with 5% freeze dried pomegranate seeds, the third group fed on 20 ppm dimethoate contaminated diet and the last group fed on dimethoate contaminated diet supplemented with 5% freeze dried pomegranate seeds. The results revealed that administration of dimethoate caused high significant increased in liver functions: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities as well as lipid peroxide (malonaldhyde, MDA); on the other hand high significant decreased on glutathione (GSH), glutathione peroxidase (GPx), albumin and total protein were observed. However addition of 5% freeze dried pomegranate seeds significantly improved all previously mentioned parameters. These results indicate the dimethoate induced hepatotoxicity and highlight the protective effect of pomegranate seeds as a potential protective agent against dimethoate induced hepatotoxicity. This may be attributed to the powerful antioxidants (polyphenols, total phenols, and total flavonoids) which present in high levels in pomegranate as well as improving the immunity by activation of antioxidant enzymes GSH and GPx.
Abstract: The protective effect of hesperidin was investigated in
rats exposed to liver injury induced by a single intraperitoneal
injection of cyclophosphamide (CYP) at a dose of 150 mg kg-1.
Hesperidin treatment (100 mg kg-1/day, orally) was applied for seven
days, starting five days before CYP administration. Hesperidin
significantly decreased the CYP-induced elevations of serum alanine
aminotransferase, and hepatic malondialdehyde and myeloperoxidase
activity, significantly prevented the depletion of hepatic glutathione
peroxidase activity resulted from CYP administration. Also,
hesperidin ameliorated the CYP-induced liver tissue injury observed
by histopathological examination. In addition, hesperidin decreased
the CYP-induced expression of inducible nitric oxide synthase, tumor
necrosis factor-α, cyclooxygenase-2, Fas ligand, and caspase-9 in
liver tissue. It was concluded that hesperidin may represent a
potential candidate to protect against CYP-induced hepatotoxicity.
Abstract: Bisphenol-A (BPA) is one of the highest volume chemicals produced worldwide in the plastic industry. This compound is mostly used in producing polycarbonate plastics that are often used for food and beverage storage, and BPA is also a component of epoxy resins that are used to line food and beverage containers. Studies performed in this area indicated that BPA could be extracted from such products while they are in contact with food. Therefore, BPA exposure is presumed. In this paper, the chemical structure of BPA, factors affecting BPA migration to food and beverages, effects on health, and recent regulations will be reviewed.
Abstract: Since nickel is a known toxic and carcinogenic metal, the present study was designed to evaluate the level of nickel released into the saliva of orthodontic patients. Non-stimulated saliva was collected from 18 patients attending The Orthodontic Clinic of Dental Faculty of Benghazi University. Patients were divided into two groups and level of nickel was determined by atomic absorption spectrophotometry. Nickel concentration value (mg/L) in first group prior to starting treatment was 0.097± 0.071. An increase in level of nickel was followed by decrease 4 and 8 weeks after applying the arch wire (0.208± 0.112) and (0.077±0.056 mg/L) respectively. Nickel levels in saliva of the second group were showed minimal variation and ranged from 0.061± 0.044mg/L to 0.083±0.054 throughout period of study. It may be concluded that there could be a release of nickel from the appliances used in first group but it doesn't reach toxic level in saliva.
Abstract: Research on the boron (B) toxicity problems had recently considerable relation, especially in the dry regions of the world. Development of resistant varieties to B toxicity is a high priority on these regions, where the soils have high levels of B. Thus, this study aimed to assessment the resistance of wheat genotypes to B toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. Durum were used. Boron fertilizer at the levels of 0 (-B), 30 mg B kg-1 (+B) as H3BO3 was applied to the pots. After harvest, plant dry matter yield was recorded, and total B concentrations in tops of wheat plants were determined. The results have revealed the existence of a large genotypic variation among wheat genotypes to their physiologic and agronomic susceptibility to B toxicity.
Abstract: Acute toxicity of nano SiO2, ZnO, MCM-41 (Meso
pore silica), Cu, Multi Wall Carbon Nano Tube (MWCNT), Single
Wall Carbon Nano Tube (SWCNT) , Fe (Coated) to bacteria Vibrio
fischeri using a homemade luminometer , was evaluated. The values
of the nominal effective concentrations (EC), causing 20% and 50%
inhibition of biouminescence, using two mathematical models at two
times of 5 and 30 minutes were calculated. Luminometer was
designed with Photomultiplier (PMT) detector. Luminol
chemiluminescence reaction was carried out for the calibration graph.
In the linear calibration range, the correlation coefficients and
coefficient of Variation (CV) were 0.988 and 3.21% respectively
which demonstrate the accuracy and reproducibility of the instrument
that are suitable. The important part of this research depends on how
to optimize the best condition for maximum bioluminescence. The
culture of Vibrio fischeri with optimal conditions in liquid media,
were stirring at 120 rpm at a temperature of 150C to 180C and were
incubated for 24 to 72 hours while solid medium was held at 180C
and for 48 hours. Suspension of nanoparticles ZnO, after 30 min
contact time to bacteria Vibrio fischeri, showed the highest toxicity
while SiO2 nanoparticles showed the lowest toxicity. After 5 min
exposure time, the toxicity of ZnO was the strongest and MCM-41
was the weakest toxicant component.
Abstract: Plant growth is affected by the osmotic stress as well as toxicity of salt in leaves. In order to study of salt stress effects on stomatal conductance and growth rate and relationship between them as wells osmotic and Na+-specific effects on these traits, four bread wheat genotypes differing in salt tolerance were selected. Salinity was applied when the leaf 4 was fully expanded. Sodium (Na+) concentrations in flag leaf blade at 3 salinity levels (0, 100 and 200 mM NaCl) were measured. Salt-tolerant genotypes showed higher stomatal conductance and growth rate compared to salt-sensitive ones. After 10 and 20 days exposure to salt, stomatal conductance and relative growth rate were reduced, but the reduction was greater in sensitive genotypes. Growth rate was reduced severely in the first period (1-10 days) of salt commencements and it was due to osmotic effect of salt not Na+ toxicity. In the second period (11-20 days) after salt treatment growth reduced only when salt accumulated to toxic concentrations in the leaves. A positive relationship between stomatal conductance and relative growth rate showed that stomatal conductance can be a reliable indicator of growth rate, and finally can be considered as a sensitive indicator of the osmotic stress. It seems 20 days after salinity, the major effect of salt, especially at low to moderate salinity levels on growth properties was due to the osmotic effect of salt, not to Na+-specific effects within the plant.
Abstract: The oleaginous yeasts Lipomyces starkey were grown
in the presence of dairy industry wastewaters (DIW). The yeasts were
able to degrade the organic components of DIW and to produce a
significant fraction of their biomass as triglycerides.
When using DIW from the Ricotta cheese production or residual
whey as growth medium, the L. starkey could be cultured without
dilution nor external organic supplement. On the contrary, the yeasts
could only partially degrade the DIW from the Mozzarella cheese
production, due to the accumulation of a metabolic product beyond
the threshold of toxicity. In this case, a dilution of the DIW was
required to obtain a more efficient degradation of the carbon
compounds and an higher yield in oleaginous biomass.
The fatty acid distribution of the microbial oils obtained showed a
prevalence of oleic acid, and is compatible with the production of a II
generation biodiesel offering a good resistance to oxidation as well as
an excellent cold-performance.
Abstract: Pyrite (FeS2) is a promising candidate for cathode
materials in batteries because of it`s high theoretical capacity, low
cost and non-toxicity. In this study, nano size iron disulfide thin film
was prepared on graphite substrate through a new method as battery
cathode. In this way, acetylene black and poly vinylidene fluoride
were used as electron conductor and binder, respectively. Fabricated
thin films were analyzed by XRD and SEM. These results and
electrochemical data confirm improvement of battery discharge
capacity in comparison with commercial type of pyrite.
Abstract: Sensitive and predictive DILI (Drug Induced Liver
Injury) biomarkers are needed in drug R&D to improve early
detection of hepatotoxicity. The discovery of DILI biomarkers that
demonstrate the predictive power to identify individuals at risk to
DILI would represent a major advance in the development of
personalized healthcare approaches. In this healthy volunteer
acetaminophen study (4g/day for 7 days, with 3 monitored nontreatment
days before and 4 after), 450 serum samples from 32
subjects were analyzed using protein profiling by antibody
suspension bead arrays. Multiparallel protein profiles were generated
using a DILI target protein array with 300 antibodies, where the
antibodies were selected based on previous literature findings of
putative DILI biomarkers and a screening process using pre dose
samples from the same cohort. Of the 32 subjects, 16 were found to
develop an elevated ALT value (2Xbaseline, responders). Using the
plasma profiling approach together with multivariate statistical
analysis some novel findings linked to lipid metabolism were found
and more important, endogenous protein profiles in baseline samples
(prior to treatment) with predictive power for ALT elevations were
identified.
Abstract: Magnetic and semiconductor nanomaterials exhibit
novel magnetic and optical properties owing to their unique size and
shape-dependent effects. With shrinking the size down to nanoscale
region, various anomalous properties that normally not present in bulk
start to dominate. Ability in harnessing of these anomalous properties
for the design of various advance electronic devices is strictly
dependent on synthetic strategies. Hence, current research has focused
on developing a rational synthetic control to produce high quality
nanocrystals by using organometallic approach to tune both size and
shape of the nanomaterials. In order to elucidate the growth
mechanism, transmission electron microscopy was employed as a
powerful tool in performing real time-resolved morphologies and
structural characterization of magnetic (Fe3O4) and semiconductor
(ZnO) nanocrystals. The current synthetic approach is found able to
produce nanostructures with well-defined shapes. We have found that
oleic acid is an effective capping ligand in preparing oxide-based
nanostructures without any agglomerations, even at high temperature.
The oleate-based precursors and capping ligands are fatty acid
compounds, which are respectively originated from natural palm oil
with low toxicity. In comparison with other synthetic approaches in
producing nanostructures, current synthetic method offers an effective
route to produce oxide-based nanomaterials with well-defined shapes
and good monodispersity. The nanocystals are well-separated with
each other without any stacking effect. In addition, the as-synthesized
nanopellets are stable in terms of chemically and physically if
compared to those nanomaterials that are previous reported. Further
development and extension of current synthetic strategy are being
pursued to combine both of these materials into nanocomposite form
that will be used as “smart magnetic nanophotocatalyst" for industry
waste water treatment.
Abstract: Spherical shaped magnetite (Fe3O4) and Au@Fe3O4
nanoparticles were successfully synthesized from Fe electrodes
immersed in water with CTAB surfactant and HAuCl4 solution using
simple method-pulsed plasma in liquid, without the use of dopants or
special conditions for stabilization. Vibrating sample magnetometer
indicated ferromagnetic behavior of particles at room temperature with
coercivity and saturation magnetization of (Hc=105 Oe, Ms=6.83
emu/g) for Fe3O4 and (Hc=175, Ms=3.56emu/g) for Au@Fe3O4
nanoparticles. Structure and morphology of nanoparticles were
characterized by X-ray Diffraction analysis and HR-TEM
measurements. The cytotoxicity of nanoparticles was indicated using a
XTT assay to be very low (cell viability: 98-89% with Fe3O4 and
99-91% for Au@Fe3O4 NPs).
Abstract: The CMLP building was developed to be a model for
sustainability with strategies to reduce water, energy and pollution,
and to provide a healthy environment for the building occupants. The
aim of this paper is to investigate the environmental effects of energy
used by this building. A LCA (life cycle analysis) was led to measure
the real environmental effects produced by the use of energy. The
impact categories most affected by the energy use were found to be
the human health effects, as well as ecotoxicity. Natural gas
extraction, uranium milling for nuclear energy production, and the
blasting for mining and infrastructure construction are the processes
contributing the most to emissions in the human health effect. Data
comparing LCA results of CMLP building with a conventional
building results showed that energy used by the CMLP building has
less damage for the environment and human health than a
conventional building.
Abstract: Carbon tetrachloride (CCl4) is a well-known
hepatotoxin and exposure to this chemical is known to induce
oxidative stress and causes liver injury by the formation of free
radicals. Flacourtia indica commonly known as 'Baichi' has been
reported as an effective remedy for the treatment of a variety of
diseases. The objective of this study was to investigate the
hepatoprotective activity of aqueous extract of leaves of Flacourtia
indica against CCl4 induced hepatotoxicity. Animals were pretreated
with the aqueous extract of Flacourtia indica (250 & 500 mg/kg
body weight) for one week and then challenged with CCl4 (1.5 ml/kg
bw) in olive oil (1:1, v/v) on 7th day. Serum marker enzymes (ALP,
AST, ALT, Total Protein & Total Bilirubin) and TBARS level
(Marker for oxidative stress) were estimated in all the study groups.
Alteration in the levels of biochemical markers of hepatic damage
like AST, ALT, ALP, Total Protein, Total Bilirubin and lipid
peroxides (TBARS) were tested in both CCl4 treated and extract
treated groups. CCl4 has enhanced the AST, ALT, ALP and the
Lipid peroxides (TBARS) in liver. Treatment of aqueous extract of
Flacourtia indica leaves (250 & 500 mg/kg) exhibited a significant
protective effect by altering the serum levels of AST, ALT, ALP,
Total Protein, Total Bilirubin and liver TBARS. These biochemical
observations were supported by histopathological study of liver
sections. From this preliminary study it has been concluded that the
aqueous extract of the leaves of Flacourtia indica protects liver
against oxidative damages and could be used as an effective protector
against CCl4 induced hepatic damage. Our findings suggested that
Flacourtia indica possessed good hepatoprotective activity