Secondary Ion Mass Spectrometry of Proteins

The adsorption of bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on fluorinated selfassembled monolayers have been studied using time of flight secondary ion mass spectrometry (ToF-SIMS) and Spectroscopic Ellipsometry (SE). The objective of the work has to establish the utility of ToF-SIMS for the determination of the amount of protein adsorbed on the surface. Quantification of surface adsorbed proteins was carried out using SE and a good correlation between ToF-SIMS results and SE was achieved. The surface distribution of proteins were also analysed using Atomic Force Microscopy (AFM). We show that the surface distribution of proteins strongly affect the ToFSIMS results.

Traffic Signal Design and Simulation for Vulnerable Road Users Safety and Bus Preemption

Mostly, pedestrian-car accidents occurred at a signalized interaction is because pedestrians cannot across the intersection safely within the green light. From the viewpoint of pedestrian, there might have two reasons. The first one is pedestrians cannot speed up to across the intersection, such as the elders. The other reason is pedestrians do not sense that the signal phase is going to change and their right-of-way is going to lose. Developing signal logic to protect pedestrian, who is crossing an intersection is the first purpose of this study. Another purpose of this study is improving the reliability and reduce delay of public transportation service. Therefore, bus preemption is also considered in the designed signal logic. In this study, the traffic data of the intersection of Chong-Qing North Road and Min-Zu West Road, Taipei, Taiwan, is employed to calibrate and validate the signal logic by simulation. VISSIM 5.20, which is a microscopic traffic simulation software, is employed to simulate the signal logic. From the simulated results, the signal logic presented in this study can protect pedestrians crossing the intersection successfully. The design of bus preemption can reduce the average delay. However, the pedestrian safety and bus preemptive signal will influence the average delay of cars largely. Thus, whether applying the pedestrian safety and bus preemption signal logic to an isolated intersection or not should be evaluated carefully.

An Approximate Solution of the Classical Van der Pol Oscillator Coupled Gyroscopically to a Linear Oscillator Using Parameter-Expansion Method

In this article, we are dealing with a model consisting of a classical Van der Pol oscillator coupled gyroscopically to a linear oscillator. The major problem is analyzed. The regular dynamics of the system is considered using analytical methods. In this case, we provide an approximate solution for this system using parameter-expansion method. Also, we find approximate values for frequencies of the system. In parameter-expansion method the solution and unknown frequency of oscillation are expanded in a series by a bookkeeping parameter. By imposing the non-secularity condition at each order in the expansion the method provides different approximations to both the solution and the frequency of oscillation. One iteration step provides an approximate solution which is valid for the whole solution domain.

Microscopic Emission and Fuel Consumption Modeling for Light-duty Vehicles Using Portable Emission Measurement System Data

Microscopic emission and fuel consumption models have been widely recognized as an effective method to quantify real traffic emission and energy consumption when they are applied with microscopic traffic simulation models. This paper presents a framework for developing the Microscopic Emission (HC, CO, NOx, and CO2) and Fuel consumption (MEF) models for light-duty vehicles. The variable of composite acceleration is introduced into the MEF model with the purpose of capturing the effects of historical accelerations interacting with current speed on emission and fuel consumption. The MEF model is calibrated by multivariate least-squares method for two types of light-duty vehicle using on-board data collected in Beijing, China by a Portable Emission Measurement System (PEMS). The instantaneous validation results shows the MEF model performs better with lower Mean Absolute Percentage Error (MAPE) compared to other two models. Moreover, the aggregate validation results tells the MEF model produces reasonable estimations compared to actual measurements with prediction errors within 12%, 10%, 19%, and 9% for HC, CO, NOx emissions and fuel consumption, respectively.

Effect of Lubrication on the Quantity of Heat Emission of two Spur Gears in Meshing

This paper investigates the effects of lubrication on the quantity of heat emission of two spur gear. System with and without lubrication effected on the quantity of heat induced on the gear box (oil - bearings – gears). Both of lubrication and speed of motor are affected on the performance of gears. Research investigated the lubrication on the system with and without loading as well as the wear of gears and bearing's conditions. Gear box investigated includes the motor, pump, two spur gears, two shafts; speed change used pulleys and belts. Load used equal one weight ones of gear. Lubrication mechanism used jet system (upper and lower jet). Gear box we used system of jet lubrication is perpendicular direction of the contact line between two teeth. Results appeared in this work that the lubrication is the vital parameter which is affected on the performance and durability of gears and bearings. In macroscopic observation, we noted that damage of bearings happened during the absence of lubrication as well as abrasive of wear of teeth. Higher speed of motor without lubrication increased the noise, but in the presence of lubrication was decreased.

Microscopic Analysis of Welded Dental Alloys

Microplasma welding is a less expensive alternative to laser welding in dental technology. The aim of the study was to highlight discontinuities present in the microplasma welded joints of dental base metal alloys by visual analysis. Five base metal alloys designated for fixed prostheses manufacture were selected for the experiments. Using these plates, preliminary tests were conducted by microplasma welding in butt joint configuration, without filler material, bilaterally and with filler material, proper for each base metal. Macroscopic visual inspection was performed to assess carefully the irregularities in the welds. Electron microscopy allowed detection of discontinuities that are not visible to the eye and revealing details regarding location, trajectory, morphology and size of discontinuities. Supplementing visual control with microscopic analysis allows to detect small discontinuities, which escapes the macroscopic control and to make a detailed study of the weld.

Knowledge Based Wear Particle Analysis

The paper describes a knowledge based system for analysis of microscopic wear particles. Wear particles contained in lubricating oil carry important information concerning machine condition, in particular the state of wear. Experts (Tribologists) in the field extract this information to monitor the operation of the machine and ensure safety, efficiency, quality, productivity, and economy of operation. This procedure is not always objective and it can also be expensive. The aim is to classify these particles according to their morphological attributes of size, shape, edge detail, thickness ratio, color, and texture, and by using this classification thereby predict wear failure modes in engines and other machinery. The attribute knowledge links human expertise to the devised Knowledge Based Wear Particle Analysis System (KBWPAS). The system provides an automated and systematic approach to wear particle identification which is linked directly to wear processes and modes that occur in machinery. This brings consistency in wear judgment prediction which leads to standardization and also less dependence on Tribologists.

Chaotic Properties of Hemodynamic Responsein Functional Near Infrared Spectroscopic Measurement of Brain Activity

Functional near infrared spectroscopy (fNIRS) is a practical non-invasive optical technique to detect characteristic of hemoglobin density dynamics response during functional activation of the cerebral cortex. In this paper, fNIRS measurements were made in the area of motor cortex from C4 position according to international 10-20 system. Three subjects, aged 23 - 30 years, were participated in the experiment. The aim of this paper was to evaluate the effects of different motor activation tasks of the hemoglobin density dynamics of fNIRS signal. The chaotic concept based on deterministic dynamics is an important feature in biological signal analysis. This paper employs the chaotic properties which is a novel method of nonlinear analysis, to analyze and to quantify the chaotic property in the time series of the hemoglobin dynamics of the various motor imagery tasks of fNIRS signal. Usually, hemoglobin density in the human brain cortex is found to change slowly in time. An inevitable noise caused by various factors is to be included in a signal. So, principle component analysis method (PCA) is utilized to remove high frequency component. The phase pace is reconstructed and evaluated the Lyapunov spectrum, and Lyapunov dimensions. From the experimental results, it can be conclude that the signals measured by fNIRS are chaotic.

Block Activity in Metric Neural Networks

The model of neural networks on the small-world topology, with metric (local and random connectivity) is investigated. The synaptic weights are random, driving the network towards a chaotic state for the neural activity. An ordered macroscopic neuron state is induced by a bias in the network connections. When the connections are mainly local, the network emulates a block-like structure. It is found that the topology and the bias compete to influence the network to evolve into a global or a block activity ordering, according to the initial conditions.

Application of H2 -based Sliding Mode Control for an Active Magnetic Bearing System

In this paper, application of Sliding Mode Control (SMC) technique for an Active Magnetic Bearing (AMB) system with varying rotor speed is considered. The gyroscopic effect and mass imbalance inherited in the system is proportional to rotor speed in which this nonlinearity effect causes high system instability as the rotor speed increases. Transformation of the AMB dynamic model into regular system shows that these gyroscopic effect and imbalance lie in the mismatched part of the system. A H2-based sliding surface is designed which bound the mismatched parts. The solution of the surface parameter is obtained using Linear Matrix Inequality (LMI). The performance of the controller applied to the AMB model is demonstrated through simulation works under various system conditions.

Is Curcumine Effect Comparable to 5- Aminosalicylic Acid or Budesonide on a Rat Model of Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid?

Inflammatory bowel disease (IBD) is a chronic relapsing-remitting condition that afflicts millions of people throughout the world and impairs their daily functions and quality of life. Treatment of IBD depends largely on 5-aminosalicylic acid (5- ASA) and corticosteroids. The present study aimed to clarify the effects of 5-aminosalicylic acid, budesonide and currcumin on 90 male albino rats against trinitrobenzene sulfonic acid (TNB) induced colitis. TNB was injected intrarectally to 50 rats. The other 40 rats served as control groups. Both 5-ASA (in a dose of 120 mg/kg) and budesonide (in a dose of 0.1 mg/kg) were administered daily for one week whereas currcumin was injected intraperitonially (in a dose of 30 mg/kg daily) for 14 days after injection of either TNB in the colitis rats (group B) or saline in control groups (group A). The study included estimation of macroscopic score index, histological examination of H&E stained sections of the colonic tissue, biochemical estimation of myeloperoxidase (MPO), nitric oxide (NO), and caspase-3 levels, in addition to studying the effect of tested drugs on colonic motility. It was found that budesonide and curcumin improved mucosal healing, reduced both NO production and caspase- 3 level. They had the best impact on the disturbed colonic motility in TNBS-model of colitis.

Influence of Cyclic Thermal Loading on Fatigue Behavior of Thermal Barrier Coatings

Thermally insulating ceramic coatings also known as thermal barrier coatings (TBCs) have been essential technologies to improve the performance and efficiency of advanced gas turbines in service at extremely high temperatures. The damage mechanisms of air-plasma sprayed YSZ thermal barrier coatings (TBC) with various microstructures were studied by microscopic techniques after thermal cycling. The typical degradation of plasma TBCs that occurs during cyclic furnace testing of an YSZ and alumina coating on a Titanium alloy are analyzed. During the present investigation the effects of topcoat thickness, bond coat oxidation, thermal cycle lengths and test temperature are investigated using thermal cycling. These results were correlated with stresses measured by a spectroscopic technique in order to understand specific damage mechanism. The failure mechanism of former bond coats was found to involve fracture initiation at the thermally grown oxide (TGO) interface and at the TGO bond coat interface. The failure mechanism of the YZ was found to involve combination of fracture along the interface between TGO and bond coat.

A Microscopic Simulation Model for Earthmoving Operations

Earthmoving operations are a major part of many construction projects. Because of the complexity and fast-changing environment of such operations, the planning and estimating are crucial on both planning and operational levels. This paper presents the framework ofa microscopic discrete-event simulation system for modeling earthmoving operations and conducting productivity estimations on an operational level.A prototype has been developed to demonstrate the applicability of the proposed framework, and this simulation system is presented via a case study based on an actual earthmoving project. The case study shows that the proposed simulation model is capable of evaluating alternative operating strategies and resource utilization at a very detailed level.

Two Dimensional Simulation of Fluid Flow and Heat Transfer in the Transition Flow Regime using a Lattice Boltzmann Approach

The significant effects of the interactions between the system boundaries and the near wall molecules in miniaturized gaseous devices lead to the formation of the Knudsen layer in which the Navier-Stokes-Fourier (NSF) equations fail to predict the correct associated phenomena. In this paper, the well-known lattice Boltzmann method (LBM) is employed to simulate the fluid flow and heat transfer processes in rarefied gaseous micro media. Persuaded by the problematic deficiency of the LBM in capturing the Knudsen layer phenomena, present study tends to concentrate on the effective molecular mean free path concept the main essence of which is to compensate the incapability of this mesoscopic method in dealing with the momentum and energy transport within the above mentioned kinetic boundary layer. The results show qualitative and quantitative accuracy comparable to the solutions of the linearized Boltzmann equation or the DSMC data for the Knudsen numbers of O (1) .

Biomechanical Properties of Hen's Eggshell: Experimental Study and Numerical Modeling

In this article, biomechanical aspects of hen-s eggshell as a natural ceramic structure are studied. The images, taken by a scanning electron microscope (SEM), are used to investigate the microscopic aspects of the egg. It is observed that eggshell has a three-layered microstructure with different morphological and structural characteristics. Studies on the eggshell membrane (ESM) as a prosperous tissue suggest that it is placed to prevent the penetration of microorganisms into the egg. Finally, numerical models of the egg are presented to study the stress distribution and its deformation under different loading conditions. The effects of two different types of loading (hydrostatic and point loadings) on two different shell models (with constant and variable thicknesses) are investigated in detail.

Internal Surface Measurement of Nanoparticle with Polarization-interferometric Nonlinear Confocal Microscope

Polarization-interferometric nonlinear confocal microscopy is proposed for measuring a nano-sized particle with optical anisotropy. The anisotropy in the particle was spectroscopically imaged through a three-dimensional distribution of third-order nonlinear dielectric polarization photoinduced.

Influence of Different Mixing Ratios of Adhesives for Wood Bondline Quality

The research study was based on an evaluation of the ability of glued test samples to pass the criterion of sufficient bondline adhesion under the exposure conditions defined in EN 302- 1. Additionally, an infrared spectroscopic analysis of the evaluated adhesives (phenol-resorcinol-formaldehyde PRF and melamine-ureaformaldehyde MUF) with different mix ratios was carried out to evaluate the possible effects of a faulty technological process.

Asymptotic Stabilization of an Active Magnetic Bearing System using LMI-based Sliding Mode Control

In this paper, stabilization of an Active Magnetic Bearing (AMB) system with varying rotor speed using Sliding Mode Control (SMC) technique is considered. The gyroscopic effect inherited in the system is proportional to rotor speed in which this nonlinearity effect causes high system instability as the rotor speed increases. Also, transformation of the AMB dynamic model into a new class of uncertain system shows that this gyroscopic effect lies in the mismatched part of the system matrix. Moreover, the current gain parameter is allowed to be varied in a known bound as an uncertainty in the input matrix. SMC design method is proposed in which the sufficient condition that guarantees the global exponential stability of the reduced-order system is represented in Linear Matrix Inequality (LMI). Then, a new chattering-free control law is established such that the system states are driven to reach the switching surface and stay on it thereafter. The performance of the controller applied to the AMB model is demonstrated through simulation works under various system conditions.

Design, Modeling and Fabrication of a Tactile Sensor and Display System for Application in Laparoscopic Surgery

One of the major disadvantages of the minimally invasive surgery (MIS) is the lack of tactile feedback to the surgeon. In order to identify and avoid any damage to the grasped complex tissue by endoscopic graspers, it is important to measure the local softness of tissue during MIS. One way to display the measured softness to the surgeon is a graphical method. In this paper, a new tactile sensor has been reported. The tactile sensor consists of an array of four softness sensors, which are integrated into the jaws of a modified commercial endoscopic grasper. Each individual softness sensor consists of two piezoelectric polymer Polyvinylidene Fluoride (PVDF) films, which are positioned below a rigid and a compliant cylinder. The compliant cylinder is fabricated using a micro molding technique. The combination of output voltages from PVDF films is used to determine the softness of the grasped object. The theoretical analysis of the sensor is also presented. A method has been developed with the aim of reproducing the tactile softness to the surgeon by using a graphical method. In this approach, the proposed system, including the interfacing and the data acquisition card, receives signals from the array of softness sensors. After the signals are processed, the tactile information is displayed by means of a color coding method. It is shown that the degrees of softness of the grasped objects/tissues can be visually differentiated and displayed on a monitor.

Attack Detection through Image Adaptive Self Embedding Watermarking

Now a days, a significant part of commercial and governmental organisations like museums, cultural organizations, libraries, commercial enterprises, etc. invest intensively in new technologies for image digitization, digital libraries, image archiving and retrieval. Hence image authorization, authentication and security has become prime need. In this paper, we present a semi-fragile watermarking scheme for color images. The method converts the host image into YIQ color space followed by application of orthogonal dual domains of DCT and DWT transforms. The DCT helps to separate relevant from irrelevant image content to generate silent image features. DWT has excellent spatial localisation to help aid in spatial tamper characterisation. Thus image adaptive watermark is generated based of image features which allows the sharp detection of microscopic changes to locate modifications in the image. Further, the scheme utilises the multipurpose watermark consisting of soft authenticator watermark and chrominance watermark. Which has been proved fragile to some predefined processing like intentinal fabrication of the image or forgery and robust to other incidental attacks caused in the communication channel.